We narrowed to 15,732 results for: LEA

1. NET31 mRFP-N2 (490)

   Plasmid

   #61984

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Malik et al PLoS One. 2014 Nov 11;9(11):e111851. doi

   Insert

   NET31 (TMEM209 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pCL1161

   Plasmid

   #217964

   Purpose

   For RAPID-release of H3-APEX-eGFP in the Flp-in T-Rex system (ThermoFisher).

   Depositor

   Andrew Bowman

   Article

   Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

   Insert

   Histone H3.1 (H3C1 Human)
   

   Tags

   eGFP, APEX

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pREDIA

   Plasmid

   #51625

   Purpose

   E. coli genome modification

   Depositor

   Sheng Yang

   Article

   Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

   Insert

   I-SceI (SCEI Budding Yeast)
   

   Use

   Red recombinase and i-scei endonuclease

   Expression

   Bacterial

   Promoter

   pRha

   Available Since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRFP-NLS

   Plasmid

   #105296

   Purpose

   To visualize nuclear dynamics in living cells

   Depositor

   Kenneth Yamada

   Article

   Petrie et al Science. 2014 Aug 29;345(6200):1062-5. d

   Insert

   Rac1 (RAC1 Human)
   

   Tags

   RFP

   Expression

   Mammalian

   Mutation

   aa encoding 172-192

   Available Since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. NET33 pEGFP-N2 (597)

   Plasmid

   #61989

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

   Insert

   NET33 (SCARA5 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDN34

   Plasmid

   #61343

   Purpose

   mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)

   Depositor

   Barbara Di Ventura, Roland Eils

   Article

   Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

   Insert

   mCherry
   

   Tags

   GS linker-AsLov2-biNLS2 and PKIt NES

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCL1324

   Plasmid

   #217965

   Purpose

   Bacterial expression of Importin-5 tagged on its N-terminus with GST. Cleavable with 3C protease.

   Depositor

   Andrew Bowman

   Article

   Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

   Insert

   Imp5 (IPO5 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   plac

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AviTag-SpyTag003-MBP

   Plasmid

   #184227

   Purpose

   Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.

   Depositor

   Mark Howarth

   Article

   Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

   Insert

   AviTag-SpyTag003-MBP
   

   Tags

   AviTag, His6, SpyTag003, and Thrombin cleavage si…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pREDKI

   Plasmid

   #51626

   Purpose

   E coli Genetic Modification

   Depositor

   Sheng Yang

   Article

   Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

   Insert

   I-SceI (SCEI Budding Yeast)
   

   Use

   Red recombinase and i-scei endonuclease

   Expression

   Bacterial

   Promoter

   pRha

   Available Since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. WN10151

    Plasmid

    #80441

    Purpose

    Expresses human codon-optimized dead (832A) LbCpf1

    Depositor

    Ervin Welker

    Article

    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

    Insert

    dead (D832A) LbCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    D832A

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-mCherry-CyrCR1

    Plasmid

    #128768

    Purpose

    Expresses mCherry in mammalian cells. ~200 nt of Cyrano inserted in 3'UTR with miR-7 site replaced by miR-17 site.

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    Cyrano conserved region (CR1) with intact miR-7 site (OIP5-AS1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPSup_35Senh(fwd)_35Spr+synJ

    Plasmid

    #149422

    Purpose

    Plasmid for STARR-seq in tobacco leaves. The GFP reporter gene is under the control of a 35S minimal promoter and an upstream 35S core enhancer.

    Depositor

    Stan Fields

    Article

    Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

    Insert

    35S core enhancer
    

    Expression

    Plant

    Available Since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTriEx-mCherry::LANS4

    Plasmid

    #60785

    Purpose

    Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

    Insert

    LANS4
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. NET29A.Hs pEGFP-N2 (1181)

    Plasmid

    #62040

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET29A (TMEM120A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Splice variant

    Promoter

    CMV

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO

    Plasmid

    #128755

    Purpose

    Expresses CYRANO gRNA for CRISPRi

    Depositor

    David Bartel

    Article

    Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

    Insert

    CYRANO sgRNA (OIP5-AS1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPAP_BGL1

    Plasmid

    #183735

    Purpose

    Expresses Aspergillus aculeatus β-glucosidase (BGL1)

    Depositor

    Jun Ishii

    Article

    Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

    Insert

    β-glucosidase (AaBGL1)
    

    Expression

    Yeast

    Promoter

    AOX1 promoter

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-HA-TRAIL-R1

    Plasmid

    #61382

    Purpose

    Expresses HA tagged TRAIL receptor 1

    Depositor

    Francis Chan

    Article

    Clancy et al Proc Natl Acad Sci U S A. 2005 Dec 13;10

    Insert

    TRAIL receptor 1
    

    Tags

    HA and TNF receptor leader peptide

    Expression

    Mammalian

    Mutation

    TRAIL-R1 leader peptide (aa1-23) replaced with TN…

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pMM0646

    Plasmid

    #129007

    Purpose

    Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker

    Depositor

    Megan McClean

    Article

    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

    Insert

    KanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
    

    Expression

    Yeast

    Available Since

    Oct. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCVL.SFFV.Kozak.HA.NLS.Y2AniK227M.IRES.BFP

    Plasmid

    #45579

    Purpose

    Expresses K227M nickase variant of I-Ani I nuclease in mammalian cells with BFP tracker

    Depositor

    Andrew Scharenberg

    Article

    Kuhar et al Nucleic Acids Res. 2013 Oct 10.

    Insert

    coY2 Ani K227M
    

    Use

    Lentiviral

    Tags

    HA, IRES BFP, and NLS

    Expression

    Mammalian

    Mutation

    co indicates codon optimization for mammalian exp…

    Promoter

    SFFV

    Available Since

    Nov. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDB22

    Plasmid

    #61342

    Purpose

    Mammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)

    Depositor

    Barbara Di Ventura, Roland Eils

    Article

    Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

    Insert

    mCherry
    

    Tags

    GS linker-AsLov2-cMyc1A NLS and PKIt NES

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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