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Plasmid#208942PurposeThe CE1 construct, expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-hISG15
Plasmid#12447DepositorInsertISG15 fragment (ISG15 Human)
UseTagsExpressionMammalianMutationISG15 up to LRLRGGPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pETM6
Plasmid#49795PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneUseTagsS-tagExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CreERT2
Plasmid#14797PurposeMammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)DepositorInsertCre-ERT2 fusion protein
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2007AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXA2
Plasmid#153110PurposeExpression of Flag tagged human FOXA2 in mammalian cellsDepositorInserthuman FOXA2 (FOXA2 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
Halo-TOMM20-N-10
Plasmid#123284PurposeLocalization: Outer Mitochondrial MembraneDepositorInsertTOMM20 (TOMM20 Human)
UseTagsHaloTag7ExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
MP1
Plasmid#69627PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 umuD' umuC recA730
UseTagsExpressionBacterialMutationPromoterE. coli PBADAvailable sinceSept. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSB700
Plasmid#64046PurposeLentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent proteinDepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6, CAGGSAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-3XHA-EGFP-PEX26
Plasmid#139054PurposeHA-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.DepositorInsert3XHA-EGFP-PEX26
UseLentiviralTagsHAExpressionMutationPromoterUBCAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-SUMO-MarathonRT
Plasmid#109029PurposeGroup II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”DepositorInsertERE_01490
UseTags6xHis-SUMO tagExpressionBacterialMutationPromoterT7Available sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ub-G76V-GFP
Plasmid#11941DepositorInsertUb-G76V-GFP
UseTagsExpressionMammalianMutationUbiquitin fused to N-terminus of GFP. Glycine 76 …PromoterAvailable sinceJune 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
hLRP1-pEZT-BM
Plasmid#177985PurposeExpression the full-length ectodomain of human LRP1 [residue 1-4414] in mammalian cellsDepositorInsertLDL receptor related protein 1 (LRP1 Human)
UseTagsNoExpressionMammalianMutationPromoterCMVAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nb-gp41-GFP (MoonTag-Nb-GFP)
Plasmid#128602PurposeExpresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).DepositorInsertNanobody_gp41
UseLentiviralTagsGB1 and sfGFPExpressionMutationPromoterSFFVAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
SaKKH-ABE8e
Plasmid#138502PurposeA-to-G base editorDepositorInsertecTadA(8e)-nSaCas9 (KKH)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S5E2-dTom-nlsdTom
Plasmid#135630PurposeAAV vector to drive the expression of dTomato in PV cortical interneurons under the control of the E2 regulatory elementDepositorHas ServiceAAV PHP.eB, AAV1, and AAV9InsertdTom-nlsdTom
UseAAVTagsExpressionMutationNonePromoterAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP8f
Plasmid#162373PurposeMammalian expression of ultrafast protein calcium sensorDepositorInsertjGCaMP8f
UseTags6xHisExpressionMammalianMutationQ315LPromoterCMVAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDY1243-Fluorescent RADARS
Plasmid#182540PurposeExpress Fluorescent RADARS in bicistronic vector with mCherry as control geneDepositorInsertmNeon
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
p5154
Plasmid#175390PurposeHigh Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts <15kb) into dual site mouse strains (Bxb-GT/GA)DepositorTypeEmpty backboneUseMouse Targeting; Cloning vector, to be used to de…TagsExpressionMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby
Plasmid#71760PurposemGFP and Synaptophysin-mRuby expression from Cre-expressing neuronsDepositorHas ServiceAAV1InsertsmGFP
Synaptophysin
UseAAV and Cre/LoxTagsPalmitoylation signal and mRubyExpressionMutationPromoterAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
mPiezo1-IRES-eGFP
Plasmid#80925Purposeexpresses mouse Piezo1 with GFPDepositorInsertmouse Piezo1 CDS (Piezo1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2016AvailabilityAcademic Institutions and Nonprofits only