We narrowed to 1,052 results for: FER

...formulation buffer is phosphate-buffered saline containing 1 mM sodium azide. This buffer is not compatible...columns. Formulation Buffer After affinity chromatography, antibodies undergo a buffer exchange using centrifugal... cells and will interfere with conjugation. For applications such as these, the buffer can be removed ...webpage. Antibody preparations developed at IPI may differ from Addgene antibodies in “Production & Usage”...extracted from cells, separated by SDS-PAGE, transferred to a membrane, and labeled with the antibody ...

...Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression with luciferase expression. Scott ...and transfer plasmids. Viral... Viral Plasmids Gamma-Retroviral Plasmids Gamma-Retroviral Plasmids Transfer Plasmids... been split into multiple plasmids for safety: Transfer plasmid — contains transgene, sgRNA, or shRNA ...collection highlights popular gamma-retroviral transfer, packaging, and envelope plasmids. Read our Gamma-retroviral...Gamma-Retroviral Vector Guide Popular Gamma-Retroviral Transfer Plasmids Want a retroviral plasmid with your gene...of interest? Search our database for retroviral transfer plasmids that have inserts. ID Plasmid 5'LTR Description...

... CRISPR Interfere CRISPR Plasmids: Interfere Mammalian Bacteria Plant Yeast Resources...knock down gene expression by interfering with transcription. This interference is most efficient when the...Overview of CRISPR interference. Browse, sort, or search the tables below for CRISPR interference plasmids. To... To learn more about CRISPR interference and other CRISPR topics, read our CRISPR Guide . Mammalian ID...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...

... mCherry Luciferase Lentiviral dual reporter vector expressing mCherry and firefly luciferase to simultaneously...105621 Lenti-luciferase-P2A-Neo Lentiviral reporter vector expressing firefly luciferase with neomycin...firefly luciferase and ZsGreen. Alejandro Balazs 170674 pBOBI-FLuc Express firefly luciferase. Used in...David Nemazee 170575 pCMV-FLuc Express firefly luciferase. Used in MLV-based SARS-CoV-2 pseudovirus assay...-GFP-Luc Lentiviral vector expressing GFP and luciferase for use as a reporter in pseudovirus production...

...for a different analyte, a sensor for gene-specific activity, or for popular biosensors in different expression...protein (cpFP) or fluorescence resonance energy transfer (FRET) efficiency of a pair of fluorescent proteins...the articles linked below include variants with different binding affinities or negative-control constructs...environmental factors and the sensor behavior may differ from the original characterization (e.g. in vitro...the plasmids associated with the article. We also offer ready-to-use AAV preparations of select plasmids...cGMP (cyclic GMP) FlincG GFP-based cGMP sensor Differential patterning of cGMP in vascular smooth muscle...chloride sensitivity, photostability and reduced ph interference demonstrates augmented transmembrane chloride...

...service gives you even more options. AAV Transfer Plasmids Transfer plasmids encode the genetic information...Browse AAV transfer, packaging, and helper plasmids in Addgene's repository. Viral...Plasmids Adeno-Associated Viral (AAV) Plasmids AAV Transfer Plasmids AAV Packaging Plasmids AAV Helper Plasmids...Plasmids Adeno-associated viral (AAV) vectors offer precise delivery of genetic material across a wide ...vectors, researchers need to use three plasmids: A transfer plasmid (or cis plasmid) containing the transgene...This collection provides a curated selection of transfer, packaging, and helper plasmids that can be used... This table contains a general list of all the transfer plasmids in Addgene's repository. You can search...

...formulated in one of the following three buffers. All buffers are supplemented with 0.001% Poloxamer 188... or 0.014% Tween 20. Phosphate Buffered Saline (PBS) A universal buffer suitable for any application Balanced...as blood, vitreous, CSF, etc. Not suitable as a buffer for vectors delivered directly to the brain parenchyma...Shannon Boye’s lab at the University of Florida. References for each serotype can be found below under Citations...is available in each of the above serotypes and buffers. Please click on the plasmid name for ordering ...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

... to target different cell populations in many brain regions. See More iPSC Differentiation Factors Find...Collection CRISPR Tools AAV Viral Preps iPSC Differentiation Factors Antibodies Fluorescent Proteins Other...Amyotrophic lateral sclerosis (ALS), sometimes referred to as Lou Gehrig’s disease, is a progressive disease...are prone to misfolding and aggregation and can interfere with protein-protein interactions and nerve cell...of these are due to a mutation in one of three different inherited genes: Presenilin 1, Presenilin 2, and...expression constructs: pLVX-UbC-rtTA-Ngn2:2A:EGFP (Schafer et al. Nat Neurosci. 2019) pLVX-UbC-rtTA-Ngn2:2A...progression of Huntington’s disease. The foundation offers curated information on tools and reagents (Link...

...transcriptional and proliferative state of each cell in a population. Proliferation history is monitored...express both components in the same relative ratio. Different generations of transactivators and promoters are...new window) for comparison of Tet-On systems in different applications. Use of Tetracycline or a Derivative...Doxycycline, a derivative of tetracycline, is a preferred effector for tet-responsive systems. Dox binds...pSBtet-GP Sleeping Beauty transposon system with luciferase in cloning site. See article ( Kowarz et al.,...172115 PB-TO-hNGN2 Piggybac Tet-On plasmid for differentiating iPSCs into glutamatergic neurons via human ...NGN2 into AAVS1 safe harbor site, for iPSC differentiation into cortical neurons Michael Ward 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA...

...more about the different types of viral vectors and find viral production and transfer plasmids for a ...Gamma-retroviral Plasmids Gamma-retroviral vectors, often referred to as just retroviral vectors, have a relatively...Viral Vector Type Each viral vector type exhibits different properties and are suited for specific research...goals. For example, AAV vectors are typically preferred for in vivo studies because of their low immunogenicity...containing the genetic information to be delivered (transfer or shuttle plasmid) and one or more plasmids containing...Specific modifications to these plasmids, such as different promoters or tags, can even direct delivery of...

... mutations in methyl-CpG-binding protein 2 confer different severity in Rett syndrome. Neurology . 70,...system for generating Rett syndrome-specific differentiated cells that can be used to understand the mechanisms...immortalized pre-neuronal cell line that can be differentiated into neurons Coriell Institute: Patient-specific... editing of mutations in the MECP2 gene. Please refer to Addgene's CRISPR Guide for a general introduction...microarray data, ISH image data, and anatomic reference atlases of prenatal and adult human brain (Link...(Link opens in a new window) Rettsyndrome.org References Amir et al. 1999. Rett syndrome is caused by ...MeCP2 levels display tissue- and cell-specific differences and correlate with neuronal maturation. Hum Mol...

... genomic engineering Luciferase Reporter pGL3 Basic Vector - Firefly luciferase vector for investigating... to create a luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase ...an insert using viral vectors, are studying a different genetic element, or want to express shRNA. Choose...investigating regions controlling transcription See our Luciferase collection of plasmid backbones into which you...target genes can be inserted into this firefly luciferase reporter to test for their effects on protein...lentiCRISPRv2 hygro - Variant of lentiCRISPR v2 that confers hygromycin resistance pRGEB32 - Express sgRNA/PTG...

...transcription factors. Addgene Blog Luciferase Technologies Enabled by NanoLuc® Luciferase Fluorescent Biosensors ...replication in many gram-negative bacteria with different purification tag combinations, including PelB ... Protein Interactions Förster resonance energy transfer (FRET) and bimolecular fluorescence complementation...that when a donor fluorophore is excited, it can transfer its energy to an acceptor fluorophore if they ...of small molecules, light, and temperature, in different bacterial systems. ID Plasmid Promoter Inducer...easily measurable reporter genes (e.g., LacZ, luciferase, or fluorescent proteins) under the control of...FFluc Promoter activity Luminescence (firefly luciferase) Mycobacterium sp. Brian Robertson , Siouxsie...

...of the applicable transfer plasmid. We will help you gain legal usage of the transfer plasmid from the ...handled at the lowest biosafety protection levels. We offer 1,077 in-stock viral vector preps, delivering high...service lets scientists choose from over 4,000 transfer plasmids to be packaged in their choice of seven...chemogenetic, optogenetic, recombinase, and biosensor transfer plasmids in our repository are eligible for this...several important steps: MTA facilitation for the transfer plasmid, including legal approval and an implementation...

...Optogenetics Ofer Yizhar 125712 pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE CamKII Inhibitor Optogenetics Ofer Yizhar...retrograde serotype from the Alla Karpova and David Schaffer laboratories. These viral vector preparations ...titering by SYBR green qPCR , confirmation of the transfer plasmid with PCR, purity assessment by silver ...pAAV-CKIIa-stGtACR2-FusionRed CamKII Inhibitor Optogenetics Ofer Yizhar 105677 pAAV_hSyn1-SIO-stGtACR2-FusionRed ...viral project team in collaboration with David Schaffer (University of California, Berkeley). Don’t See...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

...popular plasmids for gene editing in plants. Please refer to Addgene's Plant CRISPR Resources for a full selection... selection of plasmids expressing different functional variants of Cas9. ID Plasmid Description PI Genome...Resources for a selection of plasmids expressing different functional variants of Cas9. A CRISPR/Cas9 toolkit...the expression of microRNAs (miRNAs) or small interfering RNAs (siRNAs) to achieve gene silencing in plants...Complementation (rBiFC) and Förster Resonance Energy Transfer (FRET). Gateway Christopher Grefen 1000000222 .../Cas9 CRISPR-mediated Plant Base Editors RNA Interference in Plant Biology: New Tools for an Old Favorite...

...enzyme is present. Read on to learn about the different ways that Cre-lox and other site-specific recombinases...C-terminal fragments and placed under the control of different promoters. Expression of both fragments in the...the recombinase to align with your organism's preferred codon usage will improve expression. For example...of the orientation specificity of Cre and the different types of target sites available, both mutant and...such as Drosophila and mouse, particularly in proliferating cells. Expression may cause repetitive recombination...Use this AAV CRISPR knockout library expressing luciferase and Cre to interrogate tumorigenesis in vivo ...

...GSG-P2A-GCaMP6f-WPRE-pA Syn GCaMP6f mRuby2 Constitutive 1 Tobias Bonhoeffer , Mark Huebener , Tobias Rose 51083 AAV-EF1a-...-pA CAG GCaMP6f mRuby2 Cre dependent 1 Tobias Bonhoeffer , Mark Huebener , Tobias Rose 83899 pAAV-mDlx-GCaMP6f-Fishell...GSG-P2A-GCaMP6s-WPRE-pA Syn GCaMP6s mRuby2 Constitutive 1 Tobias Bonhoeffer , Mark Huebener , Tobias Rose 51082 AAV-EF1a-...-pA CAG GCaMP6s mRuby2 Cre dependent 1 Tobias Bonhoeffer , Mark Huebener , Tobias Rose 68720 pAAV-hSyn1...-pA Syn GCaMP6s mRuby2 Cre dependent 1 Tobias Bonhoeffer , Mark Huebener , Tobias Rose 100842 pAAV.CAG.Flex.GCaMP6s.WPRE.SV40... AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . ...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

...channelrhodopsin. When the luciferase enzyme oxidizes its substrate (luciferin), it generates light that...luminescent opsins: fusion proteins of a light-emitting luciferase and a light-sensing optogenetic element such ...neuron. LMO can be activated either by adding the luciferin substrate or by external illumination, making ...or other terms. Looking for viral preps? We also offer ready-to-use AAV preparations of many chemogenetics...

...reasons: Transfer plasmids with sequence from the 5′ to the 3′ ITR exceeding 4.7 kb Transfer plasmids ... Terms of Purchase and the applicable material transfer agreements (MTAs) and limited use label licenses...guaranteed here or by email, Addgene will contact you to offer some options for moving forward with a helpful solution...described here or by email, Addgene will contact you to offer some options for moving forward with a helpful solution...

...Plasmids Supplemental Information New England Biolabs offers non-toxic protein labeling systems based on SNAP...constructs are compatible with multiple applications (different fluorophores, biotin, bead purification, blocking.... For a comprehensive comparison to GFP, please refer to NEB's comparison of SNAP-tag, CLIP-tag, and GFP... is based on the human O6-alkylguanine-DNA-alkyltransferase (hAGT), a DNA repair protein, and is suitable...described previously in Stachelhaus et al. (1998) References George N, Pick H, Vogel H, Johnsson N, Johnsson...

... regulator controlling cell growth and proliferation. mTOR, a serine/threonine kinase, is found ...conditions needed for uncontrolled cell growth and proliferation, including an abundant supply of macromolecules...energy, as well as the presence of both pro-proliferative and anti-apoptotic signals. mTORC2, which appears...upstream of Akt, also promotes cell survival and proliferation. mTOR Pathway Plasmids Click on a name to find...Screening Kit (from David Sabatini & Kris Wood) References mTOR signaling in growth control and disease....

... Material Transfer Agreements Most Addgene orders must be accompanied by a material transfer agreement...agreement (MTA), including transfers to for-profit entities. The MTA is a legal agreement between the recipient... the depositing institution(s). It governs the transfer of biological materials and intellectual property...Please see the Sample Agreements on our Technology Transfer Information page for more information. To learn...

...migration, survival, adhesion, growth, and differentiation. Schematic of Ras signaling cascade that starts...ultimately turn on genes involved in cell growth, differentiation and survival. Ras is essential for regulating... related tyrosine kinase 3 FNT FNTA FNTB Farnesyltransferase, CAAX box FOS FBJ murine osteosarcoma viral...protein 2 ICMT Isoprenylcysteine carboxyl methyltransferase INSR Insulin receptor INSRR Insulin receptor-related...Initiative - at the National Cancer Institute (NCI) References Ras oncogenes and their downstream targets. Rajalingam...

... 5, 8 James M. Wilson 105532 pAAV.CMV.ffLuciferase.SV40 CMV ffLuciferase Constitutive 8 James M. Wilson...These AAV can be used to compare the activity of different serotypes. Serotype AAV1 AAV2 AAV5 AAV6 AAV8 AAV9... AAV retro helper plasmid from the Karpova and Schaffer labs. See the Retrograde AAV Inventory Page . ...re Looking For? Our Packaged on Request service offers you even more options. Select from a vast catalog...

...Phosphoprotein Reagents Phosphoprotein Synthesis Reagents References & Protocols Additional Resources Figure 1: SepRS...performing phosphoproteomic experiments in need of reference spectra or phosphopeptide standards for quantitative...The Mode #2 phosphosite library contains four different phosphobinding domains: 14-3-3β, 14-3-3σ, the ...phosphorylation positional isomers are allocated to different subpools, allowing precise knowledge of phosphorylated... SepOTSα SepRS/EF-Sep ( aka. pKD-SepRS-EFSep) References & Protocols Iterative Synthetically Phosphorylated...

...express nucleases in different cell types. Zinc finger arrays can be transferred in-frame to these vectors...plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays created using ZFC...either OPEN or modular assembly can be easily transferred in-frame to any of these vectors using unique...

... EGFP under control of human synapsin1 promotor. Ofer Yizhar 198513-AAV5 pAAV_hSyn-PdCO-EGFP-WPRE Expresses... EGFP under control of human synapsin1 promotor. Ofer Yizhar 198516-AAV1 pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE...Ef1a promoter after Cre-dependent recombination. Ofer Yizhar 198516-AAV5 pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE...Ef1a promoter after Cre-dependent recombination. Ofer Yizhar 202199-AAV1 pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE...synapsin1 promotor after Cre-dependent recombination Ofer Yizhar 202199-AAV5 pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE...synapsin1 promotor after Cre-dependent recombination Ofer Yizhar Antibodies Addgene distributes ready-to-use...

... it is the actual material being referenced in the Material Transfer Agreement (MTA) and its inclusion... Viral Vector Packaging Service Addgene offers flexible packaging options so you can easily access... vectors from the Penn Vector Core have been transferred to Addgene for distribution. Look up the IDs ...

...spectra) of different types of opsins and optical switches available in these tables, refer to Addgene'...dimerization, localization or activity of many different types of proteins. For a full description (including...or other terms. Looking for viral preps? We also offer ready-to-use AAV preparations of many optogenetics...

...Zebrafish Tools in our Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction...Ac/Ds transgenic system enabling CRISPR/dCas9-interference (CRISPRi) modulation of enhancers and non-coding...previously resistant cell types. CreLite - George Eisenhoffer Lab. An optogenetically controlled Cre/loxP system... Zebrafish Genome Project - Access the latest reference sequence assembly for the zebrafish genome. Zebrafish...

...Plasmid Collection INTRSECT Implementation Plasmids References The development of powerful molecular reagents...pAAV-Ef1a-Con/Fon/Von-GCaMP6M Cre AND Flp AND Vcre No References Fenno LE, Mattis J, Ramakrishnan C, Hyun M, Lee...Conzelmann KK, Staiger JF. 2019. Mapping Brain-Wide Afferent Inputs of Parvalbumin-Expressing GABAergic Neurons...Link opens in a new window) Poulin JF, Caronia G, Hofer C, Cui Q, Helm B, Ramakrishnan C, Chan CS, Dombeck...

...diverse roles in processes such as cell proliferation, differentiation, inflammation, metabolism, fibrosis...pages with information on available plasmids, references, and links. KLF1 KLF2 KLF4 KLF5 KLF6 KLF Plasmids...

...CRISPR Plasmids: Plants Activate Base Edit Cut Interfere Nick Prime Edit Resources The following CRISPR...Selectable Marker PI Publication Return to top Interfere ID Plasmid Gene/Insert Promoter Selectable Marker...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...

...CRISPR Plasmids: Yeast Activate Base Edit Cut Interfere Nick Purify Resources The following CRISPR plasmids...Selectable Marker PI Publication Return to top Interfere ID Plasmid Gene/Insert Promoter Selectable Marker...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...

...CRISPR Plasmids: Bacteria Activate Base Edit Cut Interfere Nick Prime Edit Purify RNA Targeting Resources.../Insert Promoter PI Publication Return to top Interfere ID Plasmid Gene/Insert Promoter PI Publication...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...

...resistance. Protocols & References A transcription factor atlas of directed differentiation. Joung J, Ma S, Tay...The Human TFome Library Stem Cells Protocols & References MORF Plasmids MORF Pooled Library The MORF library...

...Expression Activate Base Edit Cut Epigenetics Interfere Nick Prime Edit Purify RNA Editing RNA Knockdown...Selectable Marker PI Publication Return to top Interfere ID Plasmid Gene/Insert Promoter Selectable Marker...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...

...the simplicity of gRNA design, programmable for different genomic loci, capable of detecting multiple genomic...fluorescence in situ hybridization (FISH), CRISPR imaging offers a unique method for detecting the chromatin dynamics...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...

...Service Details Plasmids Available FAQ Addgene is offering Cloning Grade DNA (cgDNA) for over 200 plasmids...Volume 20 µl Concentration 100 ng/µl (± 5 ng/µl) Buffer 10 mM Tris-Cl, pH 8.5 Price Please log in to your... methods of quantification may yield slightly different DNA concentrations. Can I directly transfect cloning...

...instructors teaching different types of courses, at any type of institution, and at different points in their...regional hubs, access a teaching mentor, and more. References for teachers Using Bioinformatics and Molecular...

...Microtubules 101786 ST6GAL1-mEGFP AICSDP-26 mEGFP Sialyltransferase 1 Golgi 109122 NPM1-mEGFP AICSDP-50 mEGFP ...CTCF-binding sites 170818 PCNA-mEGFP AICSDP-116 mEGFP Proliferating cell nuclear antigen (PCNA) DNA replication ... human iPS cells (Link opens in a new window) References Roberts, B., Haupt, A., Tucker, A., Grancharova...

...Background p53 Pathway Plasmids p53 Gene List References Background p53 is a transcription factor and ...HPV, the human papillomavirus). These mutations interfere with p53’s ability to activate transcription, ...sclerosis 2 TSP1 Thrombospondin 1 Return to top References Unravelling mechanisms of p53-mediated tumour...

.... By using different ligand-dye conjugates, you can label one fusion protein with different colors as ...Explore empty plasmid backbones with different fluorescent tags to create fusion proteins with your gene...Michael Davidson Fluorescent Protein Collection Luciferase Plasmid Collection Promega Plasmid Collection...

...Plasmids GTPase Plasmids GAP and GEF Plasmids References The ARF (ADP-ribosylation factor) family of regulatory...roles). A compatible GEF, GTPase, and GAP set is referred to as a minimal triad, with each component involved...was generated with the help of Richard Kahn . References Klinger, C. M., Spang, A., Dacks, J. B., & Ettema...

... pseudotyped virus. It also lists several luciferase and fluorescent reporter plasmids that have been...LitCovid (NCBI/PubMed) (Link opens in a new window) References Andersen, K. G., Rambaut, A., Lipkin, W. I., ..., S. (2014). TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry...

...Prime Edit Transcriptional Regulation Activate Interfere Epigenetics RNA Targeting RNA Targeting RNA Editing...throughput over traditional tagging methods. Several different tagging techniques, as well as the plasmids and...Note that the principle of the PITCh system is different from the conventional HDR-mediated gene knock-...

...the genome based on their differential chromatin accessibility across different cell types and cloned into...plasmids to target a variety of cell populations in different brain regions. Labeled cell populations were tested...

...with endogenously-tagged gene variants or to differentiate human iPSCs into other cell types, as noted ...CARD) (Link opens in a new window) at the NIH. Reference Ramos et al. 2021. Tackling neurodegenerative ...

...heritable in dividing cells. CRISPR activation and interference technologies also indirectly modify local chromatin...list of CRISPR resources by visiting our CRISPR reference page . CRISPR Guide Viral Preps CRISPR Protocols...