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We narrowed to 63,771 results for: cloning

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Showing: 181 - 200 of 63771 results

  1. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid
    #73797
    Purpose
    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Publication
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73797-plasmid-map-sequence-id-196924

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  4. mEmerald-C1

    Plasmid
    #53975
    Purpose
    Localization: C1 Cloning Vector, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mEmerald
    Expression
    Mammalian
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    53975-plasmid-map-sequence-id-162690

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  5. eSpCas9(1.1)

    Plasmid
    #71814
    Purpose
    Expresses high specificity SpCas9. Px330-like plasmid.
    Depositor
    Feng Zhang
    Publication
    Slaymaker et al Science. 2015 Dec 1. pii: aad5227.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    enhanced specificity Cas9 (1.1)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    K848A, K1003A, & R1060A
    Promoter
    CBh
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71814-plasmid-map-sequence-id-177023

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  9. pCBL101.1

    Plasmid
    #199724
    Purpose
    A pVS1-based T-DNA binary vector with NPTII as a plant selectable marker gene. BsaI site was removed.
    Depositor
    Kan Wang
    Publication
    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:
    Insert
    Multiple Cloning Site, NPTII
    Expression
    Plant
    Mutation
    "GAGACC" to "GATACC"
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    199724-plasmid-map-sequence-id-391213

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  14. tdTomato-C1

    Plasmid
    #54653
    Purpose
    Localization: C1 Cloning Vector, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    tdTomato
    Expression
    Mammalian
    Available since
    June 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    54653-plasmid-map-sequence-id-450928

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  15. pCMV-dR8.2 dvpr

    Plasmid
    #8455
    Purpose
    2nd* generation lentiviral packaging plasmid. (*See comments section.) Can be used with 2nd and 3rd generation transfer vectors. Use in conjunction with an envelope plasmid such as pCMV-VSV-G.
    Depositor
    Bob Weinberg
    Publication
    Stewart et al RNA 2003 Apr;9(4):493-501.
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Available since
    June 20, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8455-plasmid-map-sequence-id-370240

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Showing: 181 - 200 of 63771 results