Addgene

We narrowed to 13,155 results for: Mpl;

Showing: 3961 - 4000 of 13155 results

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pMET7-GAG-HRAS

Plasmid

#80604

Purpose

Mammalian expression vector that expresses the HIV-1 GAG protein with HRAS fused to it

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

HIV-1 p55 GAG

Use

Tags

Expression

Mammalian

Mutation

Promoter

SRalpha

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-ULK1 (836-1050)-MBP

Plasmid

#203562

Purpose

Mammalian expression of ULK1 (836-1050) with MBP tag

Depositor

James Hurley

Article

Chen et al bioRxiv 2023.06.01.543278

Insert

ULK1 (ULK1 Human)

Use

Tags

MBP-10His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAd-Track SIRT1

Plasmid

#8440

Depositor

Pere Puigserver

Article

Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

Insert

SIRT1 (Sirt1 Mouse)

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only

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Actin-53a-ires-EGFP

Plasmid

#25460

Depositor

Jerry Crabtree

Article

Wu et al Neuron. 2007 Oct 4. 56(1):94-108.

Insert

BAF53a (Actl6a Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 14, 2010

Availability

Academic Institutions and Nonprofits only

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pCFJ2359 - intron GG2 - 250bp

Plasmid

#159878

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG2 - 250bp

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLV CDHblast Rev-EGFP-V5

Plasmid

#175164

Purpose

Lentiviral expression of Rev-EGFP-V5

Depositor

Larry Gerace

Article

Hammond et al J Mol Biol. 2018 Feb 16;430(4):537-553.

Insert

rev

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ2369 - intron GG1 - 250bp

Plasmid

#159877

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG1 - 250bp

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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HA-hAtg13

Plasmid

#31967

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

Atg13 (ATG13 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV, SP6

Available since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
pJH645

Plasmid

#172169

Purpose

Reporter plasmid for pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Pem7 luxCDA(B.1TAG)E; Plpp pylS; PproK pylT

Use

Tags

Expression

Bacterial

Mutation

Inserted amber codon immediately after initiator …

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCFJ1149 - intron 3 smu-2 PATCs

Plasmid

#161516

Purpose

PATC-rich intron number three from smu-2 to stimulate germline expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron 3 from smu-2

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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YSBE 524 : pNEO_mCherry

Plasmid

#92084

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; mCherry tag, Neo selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

mCherry

Use

tagging proteins in cryptococcus neoforman

Tags

Expression

Mutation

Promoter

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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STAGR_gRNAScaffold_h7SK

Plasmid

#102842

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

gRNAScaffold_h7SKpromoter

Use

As pcr template

Tags

Expression

Mutation

Promoter

h7SK

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-NPM NLS1/2D

Plasmid

#13287

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of aa134-aa142, aa150-aa155

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
Flag-p50(1-435)

Plasmid

#44747

Depositor

Gourisankar Ghosh

Article

Savinova et al Mol Cell. 2009 Jun 12;34(5):591-602. doi

Insert

p50 (Nfkb1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

AA 1-435

Promoter

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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GL2 shRNA

Plasmid

#86083

Purpose

A lentiviral construct for building stable cell lines expressing GL2 shRNA via puromycin selection.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

control shRNA

Use

Tags

Expression

Mutation

Promoter

U6

Available since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBrain-GFP-shGL2

Plasmid

#60004

Purpose

Co-expresses control GL2 shRNA and GFP.

Depositor

Stephen Royle

Article

Booth et al EMBO J. 2011 Mar 2;30(5):906-19. doi: 10

Insert

EGFP

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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GFP-NPM NESM-NLS1D

Plasmid

#13288

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

L100A, L102A, deletion of aa134-aa142

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pLKO.1 Blast NT shRNA

Plasmid

#123306

Purpose

Expression of a non-targeting shRNA

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

Non-targeting shRNA

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPR_DMS3g24

Plasmid

#89244

Purpose

Plasmid encodes a synthetic CRISPR containing 7 copies of a single spacer, which targets a region (gene 24) in the genome of bacteriophage DMS3

Depositor

Blake Wiedenheft

Article

Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

Insert

CRISPR

Use

Tags

Expression

Mutation

Promoter

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pJZC518

Plasmid

#62277

Purpose

Yeast dCas9 expression plasmid

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

dCas9

Use

Tags

Expression

Yeast

Mutation

Has mutations in the RuvC1 and HNH domains to ren…

Promoter

pTdh3

Available since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA-DEST EIF2C4

Plasmid

#19890

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Oct 31. ():.

Insert

Eukaryotic translation initiation factor 2C, 4 (AGO4 Human)

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pCS2+MT-hToca-1-W518K

Plasmid

#33031

Depositor

Marc Kirschner

Article

Ho et al Cell. 2004 Jul 23;118(2):203-16.

Insert

Toca-1 (FNBP1L Human)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

W518K

Promoter

simian CMV IE94

Available since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pMaCTag-P25

Plasmid

#120036

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with YFP-LID

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

YFP-LID

Use

Pcr template

Tags

YFP-LID

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFJ2370 - intron GG3 - 250bp

Plasmid

#159879

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG3 - 250bp

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPC1/GgVcl 1-258 (aka VD1)

Plasmid

#46270

Depositor

Susan Craig

Article

Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

Insert

Vcl 1-258 (aka VD1) (VCL Chicken)

Use

Tags

EGFP

Expression

Mammalian

Mutation

1-258 (VD1 domain)

Promoter

CMV

Available since

July 11, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21c-IF3mt

Plasmid

#31078

Depositor

Linda Spremulli

Article

Koc et al J Biol Chem. 2002 Sep 20. 277(38):35541-

Insert

Mature form human mitochondrial translational initiation factor 3 (MTIF3 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Includes coding sequence for the mature form. Ami…

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pEBB-II-I-GFP alpha isoform

Plasmid

#22146

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pEGFPC1/GgVcl 1-851 (Vh)

Plasmid

#46268

Depositor

Susan Craig

Article

Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

Insert

Vcl 1-851 (VCL Chicken)

Use

Tags

EGFP

Expression

Mammalian

Mutation

1-851 (Vh) head domain

Promoter

CMV

Available since

July 11, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V3)

Plasmid

#55223

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

SNAPf and TwinStrepII

Expression

Insect

Mutation

Promoter

Available since

July 9, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pSplit-OC-U5-40K

Plasmid

#51741

Purpose

Express U5-40K-LucC fusion protein in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

U5-40K (SNRNP40 Human)

Use

Tags

FLAG and Luciferase (394-550aa)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2014

Availability

Academic Institutions and Nonprofits only
pMaCTag-P02

Plasmid

#120013

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TagBFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

TagBFP

Use

Pcr template

Tags

TagBFP

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-NPM NESD

Plasmid

#13283

Depositor

Xin Wang

Article

Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

Insert

NPM (NPM1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Deletion of aa94-aa102

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pMaCTag-Z11

Plasmid

#120054

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet-i

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet-i

Use

Pcr template

Tags

mScarlet-i

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP/WT SP M85

Plasmid

#89317

Purpose

Expression of mouse GFP/WT spastin M85 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2008 Feb 27;28(9):2147-57. d

Insert

SPAST M85 (Spast Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLNHA-C1-HsUPF2-siRNAres_I

Plasmid

#146567

Purpose

Mammalian Expression of HsUPF2-siRNAres

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsUPF2-siRNAres (UPF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBG-TFII-I gamma

Plasmid

#22156

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Use

Tags

GST and His

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 9, 2009

Availability

Academic Institutions and Nonprofits only
pMaCTag-Z09

Plasmid

#120052

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mVenus

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mVenus

Use

Pcr template

Tags

mVenus

Expression

Mutation

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-ELYS-emerin-EGFP-polyA

Plasmid

#59745

Purpose

Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

ELYS-emerin-EGFP

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-YL1

Plasmid

#15333

Depositor

Joan Conaway, Ronald Conaway

Article

Cai et al J Biol Chem. 2005 Apr 8. 280(14):13665-7

Insert

transcription factor-like 1 (VPS72 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pEBB-II-I-GFP gamma isoform

Plasmid

#22148

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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