We narrowed to 10,323 results for: Coli
-
Plasmid#127829PurposeProduction of recombinant human ribosomal protein S10DepositorAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only
-
pNP1830
Plasmid#222728PurposepNP561 - T5 Target with T7 Inducible IS621 RecombinaseDepositorInsertIS621 Recombinase
ExpressionBacterialAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
hDHFR
Plasmid#184707Purposebacterial expression of human DHFRDepositorAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-PsppA-mCherry
Plasmid#225428PurposePlasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherryDepositorInsertmCherry, Cas9, SgRNA
UseCRISPRExpressionBacterialPromoterPsppA upstream of mCherry and Cas9, P3 upstream o…Available SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
J23100_GB
Plasmid#65983PurposeMoClo Basic Part: Constitutive promoter - Anderson series - high strength [G:J23100:B]DepositorInsertConstitutive promoter
UseSynthetic BiologyAvailable SinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSFDUET-1_VNp-anti-6xHis mAb
Plasmid#214952PurposeBacterial expression of anti-6xHis mAb light and heavy chain VNp fusions.DepositorInsertsVNp-anti-6xHis mAb heavy chain
VNp-anti-6xHis mAb light chain
TagsVNp6ExpressionBacterialPromoterT7Available SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNP566
Plasmid#222724PurposepNP561 - IS621 WT Target with T7 Inducible IS621 RecombinaseDepositorInsertIS621 Recombinase
ExpressionBacterialAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-PheRS
Plasmid#124116PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
prCAG-MS2
Plasmid#191121PurposeTranscription of 47xCAG and 12xMS2 repeats from a tetracycline-inducible promoterDepositorInsertsrCAG
MS2
UseSynthetic BiologyPromoterTetAvailable SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15bpATnp
Plasmid#121137PurposeThis vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.DepositorInsertProtein A-Tnp
TagshisExpressionBacterialPromoterT7Available SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-GlyRS-His
Plasmid#124110PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
placZα-tdMCP
Plasmid#191132PurposeExpression of tdMCP from a lacZα inducible promoterDepositorInsertdMCP_1
UseSynthetic BiologyPromoterlac-inducible promoterAvailable SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 FLIPE-1m
Plasmid#13544DepositorInsertFLIP-E A207W
TagsECFP and VenusExpressionMammalianMutationA207W in binding proteinAvailable SinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLG011 (DRT type 3)
Plasmid#157889PurposeExpresses the DRT type 3 defense systemDepositorInsertDRT type 3 (RT + RT)
ExpressionBacterialAvailable SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBA1802
Plasmid#246067PurposeBacterial expression of mutant T4 Acb1 (H44A,H113A) under control of the pJex promoterDepositorInsertAnti CBASS 1
ExpressionBacterialMutationH44A,H113APromoterpJEX promoterAvailable SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDF-His_TEV_MsbA
Plasmid#191476PurposeExpresses MsbA with a TEV protease cleavable N terminal His TagDepositorInsertMsbA
TagsHisExpressionBacterialPromoterT7Available SinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLG012 (DRT type 4)
Plasmid#157890PurposeExpresses the DRT type 4 defense systemDepositorInsertDRT type 4
ExpressionBacterialAvailable SinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMBA329
Plasmid#214749PurposeHigh copy glmS-nadE plasmid containing mCherry and no antibiotic resistance gene (ARG)DepositorInsertBBa_J23119-RBS(22821)-mcherry
ExpressionBacterialAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-MevT(CO)-MBIS(CO, ispA)
Plasmid#35151DepositorInsertAtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA
UseSynthetic BiologyExpressionBacterialMutationR364S in Mevalonate KinasePromoterLacUV5,Available SinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-β-Lactamase-SpyCatcher
Plasmid#52656PurposeExpresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.DepositorInsertTEM-1 β-Lactamase
TagsSpyCatcher and SpyTagExpressionBacterialPromoterT7 promoterAvailable SinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLP2
Plasmid#209989PurposeEntry vector to store Level 0 resistance cassette parts and E. coli shuttle part (containing no E. coli ori)DepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSLC-217
Plasmid#73163PurposeTemplate plasmid which encodes a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) to be used as a negative selection marker.DepositorAvailable SinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKSI-1
Plasmid#51725Purposecloning backboneDepositorTypeEmpty backboneExpressionBacterialPromoterT7Available SinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
aav-CAG-tdNfsB(F124W)-mCherry (eNTR)
Plasmid#113761PurposeAAV-mediated expression of bacterial tdNfsB-mCherry (eNTR) under the CAG promoterDepositorInserttdNfsB(F124W)-mCherry
UseAAVTagsmCherryExpressionMammalianMutationF124WPromoterCAGAvailable SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSFDUET-1_VNp-anti-GFP mAb
Plasmid#214955PurposeBacterial expression of anti-GFP mAb light and heavy chain VNp fusions.DepositorInsertsVNp-anti-GFP mAb heavy chain
VNp-anti-GFP mAb light chain
TagsVNp6ExpressionBacterialPromoterT7Available SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_mNeongreen
Plasmid#182413PurposeBacterial expression of mNeongreenDepositorInsertmNeongreen
ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPBCAG-rtTAM2-IH
Plasmid#140827PurposeExpresses rtTAM2-IRES-HygR in mammalian cellsDepositorInsertsrtTAM2
HygR
ExpressionMammalianAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
P3U2C7T2-v1-1 (Pl-lacO1-deGFP) (134p)
Plasmid#70210Purpose1 Part of 4-part switchDepositorInsertdeGFP
Available SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b T7pCONS TIR-2 sfGFP
Plasmid#154468PurposepET15b with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
TagsHis-thrombinExpressionBacterialPromoterT7Available SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSFDUET-1_VNp-anti-Lysozyme fAb
Plasmid#214951PurposeBacterial expression of anti-chicken lysozyme (folded) Fab light and heavy chain VNp fusionsDepositorInsertsVNp-anti-Lysozyme Fab heavy chain fusion
VNp-anti-Lysozyme Fab light chain fusion
TagsVNpExpressionBacterialPromoterT7Available SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSNA
Plasmid#135192PurposeArabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassetteDepositorInsertI-SceI lamba Red
UseSynthetic BiologyExpressionBacterialMutationNonePromoteraraBADAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP1
Plasmid#71465PurposeEncodes human full length SENP1 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 1 (SENP1 Human)
TagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET30a-GlmU-NahK-ET64
Plasmid#234663PurposeExpression an ELP-tagged fusion enzyme of GlmU and NahKDepositorInsertsN-acetylglucosamine- 1-phosphate uridyltransferase
N-acetylhexosamine 1-kinase
TagsELPExpressionBacterialAvailable SinceJuly 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a T7pCONS TIR-1 sfGFP
Plasmid#154463PurposepET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
TagsHis-thrombinExpressionBacterialPromoterT7Available SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast
Plasmid#213117PurposeExpression of a faster-running MinE mutant in mammalian cellsDepositorInsertMinE(L4E)-EGFP (minE Escherichia coli)
UseLentiviralExpressionMammalianMutationL4EPromoterEF1aAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-FRB-mCherry-MinD-IRES-Puro
Plasmid#213116PurposeExpression of FRB-mCherry-MinD in mammalian cells for payload deliveryDepositorAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
plYJP022-int7
Plasmid#202287PurposePlasmid containing integrase for LPattB7 siteDepositorInsertint7
ExpressionBacterialAvailable SinceSept. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAT253
Plasmid#40943DepositorInsertGFP-LacI**
TagsGFP(S65T, V163A) and SV40 NLSExpressionYeastMutationLacI**/LacI2G(4 mutations) (see comment below); l…PromoterHis3 promoter fragmentAvailable SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HsSENP2
Plasmid#71466PurposeEncodes human full length SENP2 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).DepositorInsertSUMO1/sentrin specific peptidase 2 (SENP2 Human)
TagsHexahistidineExpressionBacterialMutationGene optimised for E. coli expression (Genscript)PromoterT7Available SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJM260 (miniCTX1-rhaSR-PrhaBAD-stRBS-aacC1)
Plasmid#110572PurposeIntegration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insertDepositorInsertrhaSR-PrhaBAD-stRBS-aacC1
ExpressionBacterialPromoterrhaSR-PrhaBADAvailable SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only