We narrowed to 15,118 results for: pET

1. 3521 pET15b Bid p15

   Plasmid

   #8782

   Depositor

   Stanley Korsmeyer

   Article

   Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

   Insert

   Bid p15 (Bid Mouse)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   July 6, 2005

   Availability

   Academic Institutions and Nonprofits only

2. pET28-Nsp14-TEV-6His

   Plasmid

   #154952

   Purpose

   Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli

   Depositor

   Justin Kollman

   Insert

   Nsp14-TEV-6His (ORF1ab SARS-CoV-2)
   

   Tags

   TEV-6His

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pET-Octo_VirD2_NLS-Cas9-6xHis

   Plasmid

   #232296

   Purpose

   Expresses Cas9 protein fused with octopine-type VirD2-originating NLS

   Depositor

   Hee-Sik Kim

   Article

   Le et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

   Insert

   Octo_VirD2_NLS-Cas9-6xHis
   

   Use

   CRISPR

   Tags

   6xHis and Octopine-type VirD2-derived NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-SUMO-AsCas12f-2NLS

   Plasmid

   #171613

   Purpose

   NLS-AsCas12f1-NLS heterologous expression plasmid for RNP

   Depositor

   Quanjiang Ji

   Article

   Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

   Insert

   NLS-AsCas12f1-NLS
   

   Expression

   Bacterial

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET21b-Caspase-3(D3A,V266E)

   Plasmid

   #90091

   Purpose

   Expresses caspase D3A and V266E mutant in bacterial cells

   Depositor

   Clay Clark

   Article

   Pop et al Biochemistry. 2003 Oct 28;42(42):12311-2

   Insert

   Caspase 3 (CASP3 Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   D175A D28A D9A V266E

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-his6-HA3-SNAPf

   Plasmid

   #188941

   Purpose

   T7 expression vector for making his6- and HA3-tagged SNAP protein

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   his6-HA3-SNAPf
   

   Expression

   Bacterial

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET23b-Casp2-Delta Card

   Plasmid

   #11810

   Depositor

   Guy Salvesen

   Insert

   Caspase-2 Delta Card (CASP2 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Delta Card

   Available Since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pETM11-6xHis-TEV-hORF1p_K3AK4A

   Plasmid

   #202585

   Purpose

   Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   ORF1p
   

   Tags

   6x His and TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   Changed ORF1p Lysine 3 to Alanine, changed ORF1p …

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET22-His-TEV-DOT1L

   Plasmid

   #89610

   Purpose

   Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L residues 1-416

   Depositor

   Leemor Joshua-Tor

   Article

   Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

   Insert

   Dot1L (DOT1L Human)
   

   Tags

   His-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a(+) 6xHis-TEV-IFIT1

    Plasmid

    #53557

    Purpose

    bacterial expression of IFIT1

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT1 (IFIT1 Human)
    

    Tags

    6xHIS and TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 28, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pET28_6xHis-PARP1-CAT-L713F

    Plasmid

    #173944

    Purpose

    Bacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutation

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-CAT-L713F (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    L713F, V762A

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pet28a-ClbB-NRPS-NHis

    Plasmid

    #49217

    Purpose

    NRPS module of ClbB containing CAT domains with N-terminal 6His tag

    Depositor

    Emily Balskus

    Article

    Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

    Insert

    ClbB-NRPS
    

    Tags

    6x His tag and T7 tag

    Expression

    Bacterial

    Mutation

    contains aa4-1080 of reference sequence NP_754362…

    Promoter

    T7

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. VV020: WT Arch-3 - eGFP in pET28b

    Plasmid

    #58488

    Purpose

    Expresses WT Archaerhodopsin-3 fused to eGFP (with 6x C-terminal His tag) in E. coli

    Depositor

    Adam Cohen

    Article

    Maclaurin et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

    Insert

    Archaerhodopsin-3
    

    Tags

    eGFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-SENP6 (catalytic domain)

    Plasmid

    #16359

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP6 catalytic domain (SENP6 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 628 to 1112 catalytic domain of human …

    Available Since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pET-45b-HP1a-mCherry

    Plasmid

    #185012

    Purpose

    Bacterial Expression of 6xHis CBX5 (HP1a) mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    CBX5 (HP1a) (Cbx5 Mouse)
    

    Expression

    Bacterial

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHS0663 pET45b(+) KraIscB-1 TwinStrep

    Plasmid

    #176536

    Purpose

    Bacterial expression of KraIscB-1-TwinStrep

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    KraIscB-1
    

    Tags

    TwinStrep

    Expression

    Bacterial

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. His-SUMO-flag-hDDI2_pET11a

    Plasmid

    #169121

    Purpose

    expression of protein

    Depositor

    Barbara Dirac-Svejstrup

    Article

    Dirac-Svejstrup et al Mol Cell. 2020 Jul 16;79(2):332-341.e7.

    Insert

    hDDI (DDI2 Human)
    

    Expression

    Bacterial

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28-MBP-TEV-AICD

    Plasmid

    #69927

    Purpose

    bacterial expression of MBP-TEV-AICD

    Depositor

    Zita Balklava, Thomas Wassmer

    Article

    Currinn et al Cell Mol Life Sci. 2015 Jul 28.

    Insert

    AICD (APP Human)
    

    Tags

    MBP-TEV

    Expression

    Bacterial

    Mutation

    47 aa long

    Promoter

    T7

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-SENP8 (full length))

    Plasmid

    #16361

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP8 full length (SENP8 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    human SENP8 full length, see Depositor Comments

    Available Since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET24a HAB1 (172-511)

    Plasmid

    #177844

    Purpose

    Bacterial Expression of HAB1

    Depositor

    Karsten Melcher

    Article

    Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

    Insert

    Protein phosphatase 2C 16
    

    Tags

    6xHis-GST

    Expression

    Bacterial

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. PET-B-eSpCas9-NLS-6xHis

    Plasmid

    #207383

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-eSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A, amino acids 1005-1013 repl…

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET27b-ecDHFR-N23PP/L28F

    Plasmid

    #118584

    Purpose

    Bacterial expression of E.coli DHFR N23PP and L28F mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Insert

    DHFR
    

    Expression

    Bacterial

    Mutation

    L28 mutated to F28, N23 mutated to P23, a proline…

    Promoter

    T7

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET28a-SdrG_N2N3-HIS-ybbr

    Plasmid

    #101238

    Purpose

    SdrG N2 and N3 domains with C-terminal ybbr tag for covalent immobilization

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    SdrG_N2N3
    

    Tags

    H3V 3C, HIS, and ybbr

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pETDuet RagA(D130N)-RagC

    Plasmid

    #99642

    Purpose

    Bacterial expression of RagA(D130N)-RagC

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D130N

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET28-His6-Ubc9-K14R

    Plasmid

    #185759

    Purpose

    Bacterial expression of His6-Ubc9-K14R

    Depositor

    Jeffrey Bode

    Article

    Akimoto et al ACS Cent Sci. 2022 Feb 23;8(2):275-281.

    Insert

    SUMO-conjugating enzyme UBC9 (UBE2I Human)
    

    Tags

    Thrombin-cleavable His6-tag

    Expression

    Bacterial

    Mutation

    K14R

    Promoter

    T7

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. pET28a-TGFα-GSY-SPM

    Plasmid

    #163615

    Purpose

    Expresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.

    Depositor

    Mark Howarth

    Article

    Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

    Insert

    TGFa-GSY-SPM (TGFA Synthetic, Human)
    

    Tags

    C-tag and His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET-KIX (586-672)

    Plasmid

    #99336

    Purpose

    Bacterial expression of mouse CBP KIX domain

    Depositor

    Peter Wright

    Article

    Radhakrishnan et al J Mol Biol. 1999 Apr 16;287(5):859-65.

    Insert

    CBP KIX (586-672) (Crebbp Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PET28a-cpLumiLuc-Rapamycin sensor

    Plasmid

    #186292

    Purpose

    Expression Rapamycin sensor in e coli

    Depositor

    Kirill Alexandrov

    Article

    Guo et al Nat Commun. 2022 Feb 10;13(1):789. doi:

    Insert

    cpLumiLuc-Rapamycin sensor
    

    Tags

    HisTag

    Expression

    Bacterial

    Available Since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pETDuet RagA(T21N)-RagC

    Plasmid

    #99644

    Purpose

    Bacterial expression of RagA(T21N)-RagC

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    T21N

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-TRE-YPet-WPRE

    Plasmid

    #193342

    Purpose

    AAV-mediated expression of YPet under the TRE promoter in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

    Insert

    YPet
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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