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Ornithine transcarbamylase (OTC)

Plasmid

#71877

Purpose

For mitochondrial unfolded protein response research

Depositor

Nicholas Hoogenraad

Article

Zhao et al EMBO J. 2002 Sep 2;21(17):4411-9.

Insert

OTC (Otc Rat)

Tags

None

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti SpBsmBI sgRNA Puro

Plasmid

#62207

Purpose

An empty sgRNA expression vector for expression of sgRNA for Sp Cas9 (3rd generation lentiviral vector)

Depositor

Rene Maehr

Article

Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-RPA194

Plasmid

#17660

Depositor

Tom Misteli

Article

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Insert

RPA194 (Polr1a Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only
pCAG-mtagBFP

Plasmid

#89685

Purpose

pCAG promoter driving mtagBFP

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mtagBFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FRB-LgBiT

Plasmid

#182650

Purpose

FRB-LgBiT

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

FRB-LgBiT fusion

Tags

LgBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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p11L1w

Plasmid

#37330

Depositor

John Schiller

Article

Mossadegh et al Virology. 2004 Aug 15;326(1):57-66.

Insert

HPV11 L1

Expression

Mammalian

Promoter

EF1a

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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ECFP-TevS-YPET

Plasmid

#100097

Purpose

FRET-based ECFP-YPET reporter that is cleaved by TEV

Depositor

Jim Wells

Article

Gray et al Cell. 2010 Aug 20;142(4):637-46. doi: 10

Insert

ECFP-TevS-YPET

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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ancBE4-SpRY

Plasmid

#226855

Purpose

Expresses ancBE4max-SpRY base editor in mammalian cells with eGFP marker

Depositor

Alexis Komor

Article

Osgood et al Methods in Enzymology, February 5, 2025

Insert

ancBE4max-SpRY

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCiES

Plasmid

#60010

Purpose

Foamy virus helper plasmid expressing Env, driven by a CMV promoter. Ampicillin resistant.

Depositor

David Russell

Article

Trobridge et al Mol Ther. 2002 Sep;6(3):321-8.

Insert

Env (foamy virus)

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_CMV_MeltTEVp-40-mCherry

Plasmid

#234064

Purpose

Encodes a TEV protease controlled by a Melt variant with a switching temperature of 40°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

MeltTEV-40-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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p3s-Cas9-HN

Plasmid

#104171

Purpose

Cas9-WT mammalian expression

Depositor

Jin-Soo Kim

Article

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Insert

Cas9-WT

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR4-V5 (w71-3)

Plasmid

#17425

Purpose

Gateway entry vector for N-term V5 tag, frame A

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

V5

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pHCMV_DUGV_GP

Plasmid

#207268

Purpose

Mammalian expression of Dugbe virus GP protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

DUGV_GP

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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DECOR

Plasmid

#219545

Purpose

Expression of CMV-TadA8e-NLS-dCas13d-NLS in mammalian cells (DECOR expression)

Depositor

Weixin Tang

Article

Yan et al Nat Chem Biol. 2024 Jul 5. doi: 10.1038/

Insert

DECOR

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (+) -myc-OGA(GS-544)C181

Plasmid

#193996

Purpose

plasmid encoding myc-tagged OGA(GS-544)C181

Depositor

Christina Woo

Article

Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

Insert

myc-OGA(GS-544)C181

Tags

myc

Expression

Mammalian

Available Since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pL-RPB1

Plasmid

#10981

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Wild type RPB1 gene

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only

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mCherry-mEGFP

Plasmid

#127813

Purpose

Hetero-dimer mCherry-mEGFP expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

mCherry-mEGFP

Expression

Mammalian

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCS4092

Plasmid

#131741

Purpose

Theophylline switch Theo-CAUAA

Depositor

Christina Smolke

Article

Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

Insert

Theophylline switch Theo-CAUAA

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-iRFP720

Plasmid

#89687

Purpose

pCAG promoter driving iRFP720

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

iRFP720

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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Myo1E-pmAppleC1

Plasmid

#27698

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Myo1E (Myo1e Mouse)

Tags

mApple

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT miniAID-EGFP

Plasmid

#101713

Purpose

For amplification of a miniAID-EGFP for C-terminal tagging

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

miniAID-EGFP

Tags

miniAID-EGFP

Expression

Mammalian

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pHCMV_FLUCV_HE

Plasmid

#207275

Purpose

Mammalian expression of Influenza C virus HE protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

FLUCV_HE

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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PB-U6insert

Plasmid

#104536

Purpose

A piggybac vector with a U6 promoter that allows cloning of guide RNAs

Depositor

George Church

Article

Kalhor et al Science. 2018 Aug 9. pii: science.aat980

Type

Empty backbone

Use

CRISPR, Mouse Targeting, and Synthetic Biology ; …

Expression

Mammalian

Promoter

U6

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pHCMV_DHOV_GP

Plasmid

#207267

Purpose

Mammalian expression of Dhori virus GP protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

DHOV_GP

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_ISFV_G

Plasmid

#207282

Purpose

Mammalian expression of Isfahan virus G protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

ISFV_G

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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FUGAW

Plasmid

#129473

Purpose

3rd-generation lentiviral vector for hUbC-driven expression of GFP-aequorin

Depositor

Robert Gross

Article

Tung et al Neurophotonics. 2016 Apr;3(2):025001. do

Insert

GFP-aequorin

Use

Lentiviral

Expression

Mammalian

Promoter

human ubiquitin C

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCI-OR51E1

Plasmid

#22323

Depositor

Hiroaki Matsunami

Article

Saito et al Sci Signal. 2009 . 2(60):ra9.

Insert

OR51E1 (OR51E1 Human)

Tags

Rho

Expression

Mammalian

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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AAVS1_sgRNA

Plasmid

#100554

Purpose

Expresses AAVS1 sgRNA. Target sequence: GGGGCCACTAGGGACAGGAT

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

AAVS1 sgRNA

Promoter

U6

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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YF-Tiam1

Plasmid

#20154

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP-Tiam1 (TIAM1 Human)

Expression

Mammalian

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pCS4083

Plasmid

#131739

Purpose

Two Color Control Assay bicistronic BFP-mCherry-sTRSVctl

Depositor

Christina Smolke

Article

Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

Insert

sTRSV

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5/FRT EGFP-miniAID

Plasmid

#101714

Purpose

For amplification of a EGFP-miniAID for N-terminal tagging

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

EGFP-miniAID

Tags

EGFP-miniAID

Expression

Mammalian

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSVBneoT

Plasmid

#22883

Depositor

James Pipas

Article

Srinivasan et al Mol Cell Biol. 1997 Aug . 17(8):4761-73.

Insert

Early region SV40

Expression

Mammalian

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS4102

Plasmid

#131742

Purpose

Xanthine switch Xan-ACGAG

Depositor

Christina Smolke

Article

Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

Insert

Xanthine switch Xan-ACGAG

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
mSc-sspB

Plasmid

#114421

Purpose

Translocating to iLID domain

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRXTN

Plasmid

#47916

Purpose

pRXTN is a modified version of pRX-tight Puro (part of the two plasmid Tet-ON system from Clontech) encoding NAT instead of PAC.

Depositor

James Iglehart

Article

Kochupurakkal et al PLoS One. 2013 Jul 4;8(7):e68509. doi: 1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

Tet

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCMV_HCV_E2

Plasmid

#207280

Purpose

Mammalian expression of Hepatitis C virus E2 protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

HCV_E2

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCMV_HCV_E1

Plasmid

#207278

Purpose

Mammalian expression of Hepatitis C virus E1 protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

HCV_E1

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF-I-GFP GX

Plasmid

#45443

Depositor

John Brigande

Article

Gubbels et al Nature. 2008 Sep 25. 455(7212):537-41.

Type

Empty backbone

Tags

IRES EGFP

Expression

Mammalian

Promoter

human EF1α

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
GFP-RPA43

Plasmid

#17659

Depositor

Tom Misteli

Article

Dundr et al Science. 2002 Nov 22. 298(5598):1623-6.

Insert

RPA43 (Twistnb Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only
pHCMV_HTNV_GP

Plasmid

#207281

Purpose

Mammalian expression of Hantaan virus GP protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

HTNV_GP

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
cmv 16 E7

Plasmid

#13686

Depositor

Karl Munger

Article

Gonzalez et al J Virol. 2001 Aug . 75(16):7583-91.

Insert

HPV 16 E7 (E7 Human)

Expression

Mammalian

Available Since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
3xmEGFP

Plasmid

#127800

Purpose

Homo-trimer mEGFP-mEGFP-mEGFP expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

mEGFP-mEGFP-mEGFP

Expression

Mammalian

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Myr.PKCalpha.FLAG

Plasmid

#10807

Depositor

Alex Toker

Article

Hodges et al Adv Exp Med Biol. 2002 . 506(Pt A):237-

Insert

PKC alpha (PRKCA Bovine)

Tags

FLAG and Myr

Expression

Mammalian

Available Since

Oct. 6, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA-tdMCP-12xGCN4

Plasmid

#205158

Purpose

express tdMCP-12xGCN4

Depositor

Weirui Ma

Article

Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

Insert

tdMCP-12xGCN4

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry2-mEGFP

Plasmid

#127814

Purpose

Hetero-dimer mCherry2-mEGFP expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

mCherry2-mEGFP

Expression

Mammalian

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCD-H2B-Cherry

Plasmid

#226228

Purpose

Express H2B-Cherry

Depositor

Michael Elowitz

Article

Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

Insert

H2B-Cherry

Expression

Mammalian

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MXS_chaining vector

Plasmid

#62394

Purpose

Empty Backbone

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Wnk1

Plasmid

#38780

Depositor

Melanie Cobb

Article

Xu et al J Biol Chem. 2000 Jun 2;275(22):16795-80

Insert

Wnk1 (Wnk1 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
XLone-Puro NFIL3 P2A eGFP NLS

Plasmid

#140026

Purpose

Tunable and temporal expression control of NFIL3 and nuclear eGFP

Depositor

Xiaoping Bao

Insert

NFIL3 and enhanced Green Fluorescent Protein (NFIL3 Synthetic, Human)

Use

Synthetic Biology

Tags

eGFP

Expression

Mammalian

Promoter

TRE3G

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-mRuby2

Plasmid

#89686

Purpose

pCAG promoter driving mRuby2

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

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