We narrowed to 30,718 results for: Ide

1. LentiGuide-puro-NLS-mCherry

   Plasmid

   #185474

   Purpose

   Mammalian Expression, Lentiviral vector expressing mCherry for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. LentiGuide Puro-P2A-EGFP

   Plasmid

   #137729

   Purpose

   For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).

   Depositor

   Fredrik Wermeling

   Article

   Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   EGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDY0209 ACTB Nicking guide +48

   Plasmid

   #179109

   Purpose

   ACTB N-term Nicking guide 1 +48 guide

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   ACTB N-term Nicking guide 1 +48 guide
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiGuide-puro-NLS-GFP

   Plasmid

   #185473

   Purpose

   Mammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 1, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. Lenti-guide-puro mAtp6v1a-1

   Plasmid

   #198477

   Purpose

   lentiviral stable expression of mAtp6v1a gRNA 1

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mouse ATPv1a (Atp6v1a Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-guide-puro mAtp6v1a-2

   Plasmid

   #198478

   Purpose

   lentiviral stable expression of mAtp6v1a gRNA 2

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mouse ATPv1a (Atp6v1a Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiGuide-10X-CS1-3'-puro

   Plasmid

   #212011

   Purpose

   Deliver gRNA through lentivirus (compatible with 10x capture Sequence 1)

   Depositor

   Danwei Huangfu

   Insert

   gRNA scaffold
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Circular guide cloning vector

   Plasmid

   #180184

   Purpose

   Cloning vector for expressing circular RNA from a U6 promoter

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. LentiGuide-Puro-P2A-EGFP_mRFPstuf

   Plasmid

   #137730

   Purpose

   Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA

   Depositor

   Fredrik Wermeling

   Article

   Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, Mouse Targeting, and Syntheti…

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Level 1 P4 TaU6 guide acceptor

    Plasmid

    #165600

    Purpose

    GoldenGate (MoClo) Level 1 Position 4 TaU6 guide acceptor plasmid

    Depositor

    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

    Article

    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

    Insert

    TaU6 promoter::LacZ::sgRNA scaffold
    

    Use

    CRISPR and Synthetic Biology; Sgrna acceptor with…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Level 1 P3 TaU6 guide acceptor

    Plasmid

    #165599

    Purpose

    GoldenGate (MoClo) Level 1 Position 3 TaU6 guide acceptor plasmid

    Depositor

    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing

    Article

    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

    Insert

    TaU6 promoter::LacZ::sgRNA scaffold
    

    Use

    CRISPR and Synthetic Biology; Sgrna acceptor with…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. NPY/Neuropeptide Y scFv [L115/13]

    Plasmid

    #190505

    Purpose

    Mammalian Expression of NPY/Neuropeptide Y scFV. Derived from hybridoma L115/13.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    NPY/Neuropeptide Y (Homo sapiens) recombinant scFV (NPY Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. p3E-U6a-polg guide

    Plasmid

    #107598

    Purpose

    An entry vector with U6a promoter driving polg guide RNA expression

    Depositor

    Qing Deng

    Article

    Zhou et al Dis Model Mech. 2018 Mar 28;11(3). pii:

    Insert

    polg guide RNA sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiGuide-Puro PRMT5-CRISPRi

    Plasmid

    #164637

    Purpose

    lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2

    Depositor

    Günter Schneider

    Insert

    target guide sequence PRMT5 inhibition (PRMT5 Human)
    

    Use

    CRISPR and Lentiviral; Interference

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-guide-puro mMyh9 - 2

    Plasmid

    #198492

    Purpose

    lentiviral stable expression of mMyh9 gRNA 2

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mMyh9 (Myh9 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-guide-puro mMyh9 - 1

    Plasmid

    #198491

    Purpose

    lentiviral stable expression of mMyh9 gRNA 1

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mMyh9 (Myh9 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. AP1muA single guide RNA

    Plasmid

    #230030

    Purpose

    guide RNA for AP1muA tagging at the C-terminus in pX459

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al J Cell Sci. 2024 Aug 15;137(16):jcs26195

    Insert

    AP1M1 guide (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLQ5135: pCAG-BFP-GuideArray

    Plasmid

    #210606

    Purpose

    Guide array for hyperdCas12a-based transcriptional activator with AAAT synSeparator

    Depositor

    Stanley Qi

    Article

    Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

    Insert

    pCAG-BFP-GuideArray
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available Since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiGuide-Puro-GFPg1 (BB09)

    Plasmid

    #139461

    Purpose

    Lentiviral vector with gRNA targeting GFP; includes puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    GFP-targeting sgRNA inserted; resistance gene: puroR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. lenti-sgRNA(MS2)_zeo_hCYP3A4_enhancer_guide1

    Plasmid

    #176839

    Purpose

    To recruit SAM complex to human CYP3A4 proximal enhancer.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

    Insert

    Human CYP3A4 proximal enhancer sgRNA for activation
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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