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Plasmid#200732PurposeBacterial expression of GST-Tagged codon optimized human pS43 METTL3DepositorInsertpS43 METTL3 (METTL3 Human)
UseTagsGST from backbone plasmidExpressionBacterialMutationCodon optimized for expression in E. coli, UAG co…PromoterAvailable sinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
MET gRNA (BRDN0001147257)
Plasmid#76063Purpose3rd generation lentiviral gRNA plasmid targeting human METDepositorInsertMET (MET Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pF713-pEGFP-N1-Hs-FL-METTL20 wt
Plasmid#85114Purposetransfection of HeLa cell for transient expression of human full-length METTL20-EGFP fusionDepositorInserthuman full-length METTL20 (METTL20 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
MET gRNA (BRDN0001145767)
Plasmid#76060Purpose3rd generation lentiviral gRNA plasmid targeting human METDepositorInsertMET (MET Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MET gRNA (BRDN0001147542)
Plasmid#76062Purpose3rd generation lentiviral gRNA plasmid targeting human METDepositorInsertMET (MET Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK5-Myc-metap2
Plasmid#100516PurposeExpresse Myc tagged metap2 in mammalian cellsDepositorInsertMetap2 (METAP2 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
MET gRNA (BRDN0001147560)
Plasmid#76061Purpose3rd generation lentiviral gRNA plasmid targeting human METDepositorInsertMET (MET Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2+methylated-reporter
Plasmid#164491PurposeReporter to test methylated mRNA stability, for mRNA injectionDepositorInsertA-less CDS, HA tag, 12x m6A motif 3'UTR
UseProduction of mrna for zebrafish injection.TagsHA tagExpressionMutationPromoterSP6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
px335 Mettl14 sgRNA #1
Plasmid#61515Purposeencodes sgRNA sequence for targeting mouse Mettl14 locus (Cas9-Nickase strategy)DepositorInsertgcggcagctcctagctcagc
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSCV-PIG-METTL14-WT
Plasmid#141118PurposeRetroviral overexpression of WT METTL14 in mammalian cells.DepositorInsertMETTL14 (METTL14 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRK5 GST MetaP2
Plasmid#112750Purposeexpress HA-GST-FRB-MetaP2DepositorInsertHA-GST-FRB-MetaP2 (METAP2 Human)
UseTagsGSTExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
R777-E131 Hs.MET
Plasmid#70415PurposeGateway ORF clone of human MET [NM_000245.2] with stop codon (for native or N-terminal fusions)DepositorInsertMET (MET Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-cpVenus-Metallothionein-3
Plasmid#58176PurposeLocalization: Cytosolic FRET, Excitation: , Emission:DepositorTypeEmpty backboneUseTagscpVenus-Metallothionein and mCeruleanExpressionMammalianMutationPromoterAvailable sinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICE LYS2-MET17
Plasmid#122889PurposeLYS2-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIp ADE2-MET17
Plasmid#122906PurposeADE2-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-mEtfdh-AAA4
Plasmid#232316PurposeExpression of mouse mutant Etfdh (AAA4)DepositorInsertEtfdh (Etfdh Mouse)
UseLentiviralTagsExpressionMutationPAM Sequence Mutations: (G36C; T39C, C42T; C72T, …PromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-MET25pr-NFAPoptim
Plasmid#221101PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-MET25pr-CFAPoptim
Plasmid#221102PurposeFAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-MET25pr-NFAPoptim
Plasmid#221104PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-MET25pr-CFAPoptim
Plasmid#221105PurposeFAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only