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pgRNA-bacteria

Plasmid

#44251

Purpose

Expression of customizable guide RNA (gRNA) for bacterial gene knockdown

Depositor

Stanley Qi

Publication

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

guide RNA (bacteria)

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 (SpeI)

Available since

April 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

73 Articles

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pdCas9-bacteria

Plasmid

#44249

Purpose

aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown

Depositor

Stanley Qi

Publication

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

dCas9 (bacteria)

Use

CRISPR

Expression

Bacterial

Mutation

D10A H840A (catalytically inactive)

Promoter

pLtetO-1

Available since

April 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

82 Articles

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pCMV-PEmax

Plasmid

#174820

Purpose

Mammalian expression of SpCas9 PEmax prime editor

Depositor

David Liu

Publication

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PEmax

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

27 Articles

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piRFP670-N1

Plasmid

#45457

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

iRFP670

Expression

Mammalian

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

58 Articles

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pEF-GFP

Plasmid

#11154

Purpose

Mammalian expression vector for expression of GFP (EF1a promoter)

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EF1 alpha promoter (EEF1A1 Human)

Tags

EGFP

Expression

Mammalian

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

53 Articles

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dCas9-KRAB-MeCP2

Plasmid

#110821

Purpose

Improved dCas9 repressor-dCas9-KRAB-MeCP2

Depositor

Alejandro Chavez, George Church

Publication

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9-KRAB-MeCP2

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CMV

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

43 Articles

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pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#71237

Purpose

Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

Depositor

Charles Gersbach

Publication

Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

Inserts

humanized dCas9-KRAB T2A GFP
sgRNA

Use

CRISPR and Lentiviral

Tags

Flag

Mutation

D10A and H840A

Available since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

69 Articles

Enlarge
pgRNA-bacteria

Plasmid

#44251

Purpose

Expression of customizable guide RNA (gRNA) for bacterial gene knockdown

Depositor

Stanley Qi

Publication

Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

Insert

guide RNA (bacteria)

Use

CRISPR

Expression

Bacterial

Promoter

BBa_J23119 (SpeI)

Available since

April 5, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

73 Articles

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pcDNA3-S33Y Beta-catenin

Plasmid

#19286

Purpose

Mammalian expression of human beta-catenin with Flag tag and S33Y mutation

Depositor

Eric Fearon

Publication

Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

Insert

beta-catenin (CTNNB1 Human)

Tags

Flag

Expression

Mammalian

Mutation

S33Y (presumptive GSK3β phosphorylation site)

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

52 Articles

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pSico

Plasmid

#11578

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression.

Depositor

Tyler Jacks

Publication

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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pCAG-mGFP

Plasmid

#14757

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

membrane bound form of EGFP

Tags

Palmitoylation signal

Expression

Mammalian

Promoter

pCAG

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

57 Articles

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pEF-GFP

Plasmid

#11154

Purpose

Mammalian expression vector for expression of GFP (EF1a promoter)

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EF1 alpha promoter (EEF1A1 Human)

Tags

EGFP

Expression

Mammalian

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

53 Articles

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HRE-luciferase

Plasmid

#26731

Purpose

Luciferase reporter construct containing three hypoxia response elements (24-mers) from the Pgk-1 gene.

Depositor

Navdeep Chandel

Publication

Emerling et al Proc Natl Acad Sci U S A. 2008 Feb 19. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Hypoxia Responsive Elements (3)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

90 Articles

Enlarge
pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#71237

Purpose

Express sgRNA and dCas9-KRAB from 3rd generation lentiviral vector

Depositor

Charles Gersbach

Publication

Thakore et al Nat Methods. 2015 Dec;12(12):1143-9. doi

Inserts

humanized dCas9-KRAB T2A GFP
sgRNA

Use

CRISPR and Lentiviral

Tags

Flag

Mutation

D10A and H840A

Available since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

69 Articles

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myc-Rictor corrected

Plasmid

#11367

Purpose

Mammalian expression of myc tagged human RICTOR

Depositor

David Sabatini

Publication

Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

Insert

Rictor (RICTOR Human)

Tags

Myc

Expression

Mammalian

Available since

April 27, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

37 Articles

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pLJC5-Tmem192-3xHA

Plasmid

#102930

Purpose

Lentiviral expression of a lysosomal tag (Tmem192-HA)

Depositor

David Sabatini

Publication

Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

Insert

Tmem192 (TMEM192 Human)

Use

Lentiviral

Tags

HA

Promoter

ubc

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

69 Articles

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HA GSK3 beta S9A pcDNA3

Plasmid

#14754

Purpose

Mammalian expression of human GSK3 beta constitutively active mutant with HA tag

Depositor

Jim Woodgett

Publication

Stambolic et al Biochem J. 1994 Nov 1;303 ( Pt 3)(Pt 3):

Insert

GSK3 beta S9A (GSK3B Human)

Tags

HA

Expression

Mammalian

Mutation

S9A. Constitutively active mutant.

Available since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

68 Articles

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pSico

Plasmid

#11578

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression.

Depositor

Tyler Jacks

Publication

Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

Enlarge
pCAG-mGFP

Plasmid

#14757

Depositor

Connie Cepko

Publication

Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

membrane bound form of EGFP

Tags

Palmitoylation signal

Expression

Mammalian

Promoter

pCAG

Available since

April 20, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

57 Articles

Enlarge
HRE-luciferase

Plasmid

#26731

Purpose

Luciferase reporter construct containing three hypoxia response elements (24-mers) from the Pgk-1 gene.

Depositor

Navdeep Chandel

Publication

Emerling et al Proc Natl Acad Sci U S A. 2008 Feb 19. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Hypoxia Responsive Elements (3)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

90 Articles

Enlarge