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pET-30-His6-SUMO-AEY-CfaN

Plasmid

#122949

Purpose

Bacterial expression of N-intein

Depositor

Thomas Muir

Article

Stevens et al J Am Chem Soc. 2016 Feb 24;138(7):2162-5

Insert

CfaN

Expression

Bacterial

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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XE240 Fz1-ER-GFP

Plasmid

#16815

Depositor

Randall Moon

Article

Kaykas et al Nat Cell Biol. 2004 Jan . 6(1):52-8.

Insert

Fz1-ER-GFP

Use

Xenopus expression

Tags

gfp

Mutation

F36M in FKBP

Available Since

May 19, 2008

Availability

Academic Institutions and Nonprofits only
pTEF1_MS2

Plasmid

#220625

Purpose

Source for TEF1 MS2 coat fusion protein fusion

Depositor

Marvin Wickens

Article

Preston et al Nat Methods. 2019 May;16(5):437-445. doi

Type

Empty backbone

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-mCherry

Plasmid

#32636

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

mCherry

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-DsRed2

Plasmid

#32638

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

DsRed2

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only

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FIRES-PBCS-E670

Plasmid

#228699

Purpose

Sensor cassette FIRE2, FIRE fluorescent ratiometric sensor

Depositor

Carolyn Sangokoya

Article

Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

Insert

FIRE2 SENSOR

Use

Synthetic Biology and Unspecified

Expression

Mammalian

Promoter

hpgk

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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FIREC-PBCS-MR3

Plasmid

#228700

Purpose

Control cassette FIRE2, FIRE fluorescent ratiometric sensor

Depositor

Carolyn Sangokoya

Article

Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

Insert

FIRE2 CONTROL

Use

Synthetic Biology and Unspecified

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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MyrPalm-CKAR

Plasmid

#14862

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

C kinase activity reporter

Tags

CFP, Myr/Palm, and YFP

Expression

Mammalian

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:GFP-CAAX

Plasmid

#184813

Purpose

Used to generate the Tg(QUAS:GFP-CAAX)c591 transgenic line

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:GFP-CAAX-pA

Use

Zebrafish tol2 transgenesis

Tags

GFP-CAAX

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-GFP

Plasmid

#58867

Purpose

AAV expression of GFP under the Syn promoter

Depositor

Edward Boyden

Article

Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Insert

GFP

Use

AAV

Expression

Mammalian

Promoter

Syn

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pBabe 12S E1A

Plasmid

#18742

Depositor

Scott Lowe

Article

Samuelson et al Proc Natl Acad Sci U S A. 1997 Oct 28. 9

Insert

12S E1A

Use

Retroviral

Expression

Mammalian

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pNX24-RFP-MS2SL

Plasmid

#227819

Purpose

Expresses RFP via CMV promoter. The mRNA is tagged with 24x MS2 stem-loops (version 3) at the 3'UTR. Also encodes Neo resistance gene

Depositor

Jeffrey Gerst

Article

Haimovich et al bioRxiv 2024.11.06.622258

Inserts

3xHA-RFP-24xMS2SL
Neo resistance

Expression

Mammalian

Promoter

CMV and SV40

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pSB233

Plasmid

#164128

Purpose

PP7-CFP and PhlF-RFP expression plasmid

Depositor

Christopher Voigt

Article

Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

Insert

PP7-CFP

Expression

Bacterial

Promoter

Ptet

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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FCK-ChR2-GFP

Plasmid

#15814

Purpose

Lentiviral expression of ChR2-GFP for neuronal excitation

Depositor

Edward Boyden

Article

Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Insert

ChR2

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CaMKII

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pQCXI Puro DsRed-LC3-GFP

Plasmid

#31182

Depositor

David Sabatini

Article

Sheen et al Cancer Cell. 2011 May 17. 19(5):613-28.

Insert

DsRed-LC3-GFP

Use

Retroviral

Expression

Mammalian

Available Since

June 17, 2011

Availability

Academic Institutions and Nonprofits only

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LV-Camk2-Ace-7aa-mScarlet

Plasmid

#129701

Purpose

Expresses Ace-7aa-mScarlet in mammalian cells

Depositor

Yiyang Gong

Article

Beck et al Sci Rep. 2019 Nov 4;9(1):15878. doi: 10.

Insert

Ace-7aa-mScarlet

Use

Lentiviral

Expression

Mammalian

Promoter

Camk2

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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mCitrine-C1

Plasmid

#54587

Purpose

Localization: C1 Cloning Vector, Excitation: 516, Emission: 529

Depositor

Robert Campbell, Michael Davidson, Oliver Griesbeck, Roger Tsien

Article

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Type

Empty backbone

Tags

mCitrine

Expression

Mammalian

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pL-RPB1

Plasmid

#10981

Depositor

Jeff Strathern

Article

Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Wild type RPB1 gene

Available Since

Dec. 16, 2005

Availability

Academic Institutions and Nonprofits only

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pME-NLS-tdMCP-eGFP

Plasmid

#86243

Purpose

Middle entry vector for inserting promotors for tissue specific expression of NLS-tdMCP-eGFP reporter

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Type

Empty backbone

Use

Unspecified

Tags

NLS-tdMCP-eGFP

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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ssFv_lenti_mutATMSPARK2

Plasmid

#195250

Purpose

express Thr to Ala mutant ATMSPARK2 in a lenti vector

Depositor

Xiaokun Shu

Article

Li et al Sci Adv. 2023 Mar;9(9):eade3760. doi: 10

Insert

Thr to Ala mutant ATMSPARK2

Use

Lentiviral

Expression

Mammalian

Promoter

ssfv

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pYOY152

Plasmid

#13987

Depositor

Linda Wordeman

Article

Ovechkina et al J Cell Biol. 2002 Nov 25. 159(4):557-62.

Insert

Mitotic Centromere Associated Kinesin

Tags

GFP and His

Expression

Mammalian

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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pCMV-Rev(NL4.3)

Plasmid

#115776

Purpose

pCMV-RevNL4.3 allows the expression of Rev protein.

Depositor

Damian Purcell

Article

Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

Insert

HIV-1 Rev protein (NL4.3)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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FOP-GFP

Plasmid

#35490

Depositor

Ramesh Shivdasani

Article

Horst et al Cancer Res. 2012 Feb 8.

Insert

7xFOP-GFP

Use

Lentiviral

Expression

Mammalian

Promoter

mutated 7xTCF/LEF

Available Since

April 10, 2012

Availability

Academic Institutions and Nonprofits only

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pCS2 xenopus sFRP2

Plasmid

#24696

Depositor

Edward De Robertis

Article

Pera et al Mech Dev. 2000 Sep . 96(2):183-95.

Insert

secreted frizzled-related sequence protein 2 (sfrp2 Frog)

Expression

Mammalian

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pET28a-PFO

Plasmid

#92109

Purpose

Recombinant expression in E.Coli

Depositor

Feng Shao

Article

Ding et al Nature. 2016 Jul 7;535(7610):111-6. Epub

Insert

PFO

Tags

6xHis

Expression

Bacterial

Mutation

Delta27aa

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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Sirius-N1

Plasmid

#54506

Purpose

Localization: N1 Cloning Vector, Excitation: 355, Emission: 424

Depositor

Michael Davidson

Article

Tomosugi et al Nat Methods. 2009 May;6(5):351-3. doi: 1

Type

Empty backbone

Tags

Sirius

Expression

Mammalian

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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Fubi-ChR2-GFP

Plasmid

#22051

Purpose

Viral expression of ChR2-GFP for neuronal excitation

Depositor

Edward Boyden

Article

Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

Insert

ChR2-GFP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

N/A

Promoter

UbC

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pET28a human Tankyrase2 full length Y920E

Plasmid

#132639

Purpose

expresses human Tankyrase2 full length Y920E in E. coli

Depositor

Wenqing Xu

Article

DaRosa et al Protein Sci. 2016 Sep;25(9):1744-52. doi

Insert

Tankyrase2 Y920E

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pEZY19

Plasmid

#18668

Purpose

pET19b converted Gateway Destination vector for expressing His tagged fusion proteins in bacteria. See note in comments section below regarding the mutated enterokinase site.

Depositor

Yu-Zhu Zhang

Article

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Use

Gateway destination vector

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

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BKAR

Plasmid

#14875

Depositor

Alexandra Newton

Article

Kunkel et al J Biol Chem. 2005 Feb 18. 280(7):5581-7.

Insert

B kinase activity reporter

Tags

CFP and YFP

Expression

Mammalian

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pCS2+8NmCherry-ABCB1a

Plasmid

#34939

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Insert

ABCB1a

Use

Sea urchin, zebrafish, xenopus

Tags

mCherry

Expression

Mammalian

Promoter

SP6

Available Since

Jan. 7, 2013

Availability

Academic Institutions and Nonprofits only
CMV::ratSyGCaMP2

Plasmid

#26124

Depositor

Leon Lagnado

Article

Dreosti et al Nat Methods. 2009 Dec . 6(12):883-9.

Insert

Synaptophysin GCaMP2 (Syp Rat)

Expression

Mammalian

Available Since

Nov. 11, 2010

Availability

Academic Institutions and Nonprofits only

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Citrine-C1

Plasmid

#54715

Purpose

Localization: C1 Cloning Vector, Excitation: 516, Emission: 529

Depositor

Robert Campbell, Michael Davidson, Oliver Griesbeck, Roger Tsien

Article

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Type

Empty backbone

Tags

Citrine

Expression

Mammalian

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pQLinkHD-SYNZIP2

Plasmid

#80658

Purpose

Encodes the synthetic coiled coil peptide SYNZIP2

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP2

Tags

6xHis

Expression

Bacterial

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pQLinkHD-SYNZIP1

Plasmid

#80647

Purpose

Encodes the synthetic coiled coil peptide SYNZIP1

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP1

Tags

6xHis

Expression

Bacterial

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG504-frFAST

Plasmid

#160055

Purpose

Expresses frFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Li et al Angew Chem Int Ed Engl. 2020 Jun 22. doi

Insert

frFAST

Expression

Mammalian

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSADdeltaG-BFP

Plasmid

#32639

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

BFP

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pBD-0001

Plasmid

#12373

Depositor

Axel Brunger

Article

DeLaBarre et al Mol Cell. 2006 May 19. 22(4):451-62.

Insert

VCP (Vcp Mouse)

Tags

His6

Expression

Bacterial

Available Since

Aug. 24, 2006

Availability

Academic Institutions and Nonprofits only

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pFLuc190UGA

Plasmid

#41046

Depositor

James Inglese

Article

Auld et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Firefly luciferase 190UGA

Expression

Mammalian

Mutation

in-frame UGA stop codon was created at codon 190

Promoter

CMV

Available Since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
pBSDT-AII

Plasmid

#27179

Depositor

Yoh Wada

Article

Aoyama et al Nucleic Acids Res. 2005 . 33(5):e52.

Insert

MC1 promoter diphtheria toxin-A fragment

Use

Mouse Targeting

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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