Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

164,169 results

Showing: 5601 - 5650 of 164169 results

  8. Human SAM library in lentiSAMv2 backbone

    Pooled Library
    #1000000078
    Purpose
    Genome-wide CRISPR gRNA pooled library for activation of human genes
    Depositor
    Feng Zhang
    Available since
    June 27, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. HTR2C-Tango

    Plasmid
    #66411
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    HTR2C (HTR2C Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66411-plasmid-map-sequence-id-139337

    Enlarge

  15. pAAV-hSynapsin1-FLEx-axon-GCaMP6s (AAV9)

    Viral Prep
    #112010-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSynapsin1-FLEx-axon-GCaMP6s (#112010). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-FLEx-axon-GCaMP6s plasmid DNA. Synapsin-driven, cre-dependent axon-targeted GCaMP6s calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Lin Tian
    Article
    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.
    Promoter
    Syn
    Tags
    None
    Available since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only

  16. pAAV-Ef1a-fDIO EYFP (AAV Retrograde)

    Viral Prep
    #55641-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-Ef1a-fDIO EYFP (#55641). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO EYFP plasmid DNA. EF1a-driven, Flp-dependent expression of EYFP. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    EF1a
    Tags
    EYFP (Flp-dependent)
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  22. pCutamp

    Plasmid
    #140632
    Purpose
    Plasmid-curing in Escherichia coli by targeting the AmpR promoter
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102
    Insert
    SpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140632-plasmid-map-sequence-id-306006

    Enlarge

  23. 3xnls-mScarlet-I

    Plasmid
    #98816
    Purpose
    In vivo visualization of the nucleus (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    3xNLS
    Use
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    98816-plasmid-map-sequence-id-190172

    Enlarge

  27. pAAV-EF1a-fDIO-Cre (AAV Retrograde)

    Viral Prep
    #121675-AAVrg
    Purpose
    Ready-to-use AAV Retrograde particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Esteban Engel, Alexander Nectow
    Article
    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30
    Promoter
    EF1a
    Tags
    None
    Available since
    May 31, 2019
    Availability
    Academic Institutions and Nonprofits only

  31. pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1

    Plasmid
    #198240
    Purpose
    For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sites
    Depositor
    Didier Stainier
    Article
    Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.
    Inserts
    Zebrafish fabp10a promoter:floxedBFP, Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
    Zebrafish cryaa promoter:mcherry fluorescent protein coding sequence (cryaa Zebrafish)
    Use
    Zebrafish transgenesis
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    198240-plasmid-map-sequence-id-393627

    Enlarge

  36. pAAV.Syn.GCaMP6m.WPRE.SV40 (AAV1)

    Viral Prep
    #100841-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.Syn.GCaMP6m.WPRE.SV40 (#100841). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6m.WPRE.SV40 plasmid DNA. Syn-driven GCaMP6m calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project, Douglas Kim
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Promoter
    Syn
    Tags
    None
    Available since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only

  37. GreenGate

    Plasmid Kit
    #1000000036
    Purpose
    Golden Gate cloning system for rapid assembly of plant transformation constructs; Uses 6 types of pre-cloned modules: promoter, N- and C-terminal tags, coding sequence, terminator, resistance cassette
    Depositor
    Jan Lohmann
    Available since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only

  39. pAAV.CMV.PI.EGFP.WPRE.bGH (AAV1)

    Viral Prep
    #105530-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA. CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    CMV
    Tags
    EGFP
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only

  40. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid
    #216246
    Purpose
    Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration
    Depositor
    Elina Ikonen
    Article
    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:
    Insert
    Cas9 and sgAAVS1.5
    Use
    CRISPR; Human safe harbor locus (a avs1) site-spe…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216246-plasmid-map-sequence-id-426842

    Enlarge

  47. pAC-LYC

    Plasmid
    #53270
    Purpose
    Contains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coli
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Plant Cell. 1994 Aug;6(8):1107-21.
    Insert
    crtE, crtI, crtB
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    endogenous promoters
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53270-plasmid-map-sequence-id-82990

    Enlarge

  49. gCH130 (crCD55-4_crB2M-1_crKIT-2_crCD81-1)

    Plasmid
    #217340
    Purpose
    crRNA array targeting CD81, B2M, KIT, CD55
    Depositor
    Luke Gilbert
    Article
    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
    crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
    crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
    crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hU6
    Available since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    217340-plasmid-map-sequence-id-426478

    Enlarge

  50. NG-ABE9

    Plasmid
    #240625
    Purpose
    Adenine base editor with narrowed editing window, recognizing NG PAM
    Depositor
    Tingting Jiang
    Insert
    TadA8e (N108Q/L145T)-linker-nCas9 (D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    TadA8e (N108Q/L145T), Cas9 (D10A, L1111R, D1135V,…
    Promoter
    Available since
    July 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    240625-plasmid-map-sequence-id-479197

    Enlarge

Showing: 5601 - 5650 of 164169 results