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Showing: 5601 - 5650 of 160192 results

  3. loxP-G418-LoxP-TurboID-Rab11 HR

    Plasmid
    #230026
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    Turbo-ID, V5
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    230026-plasmid-map-sequence-id-457126

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  4. pDONR221-SLC1A4_STOP

    Plasmid
    #161128
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC1A4 (SLC1A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161128-plasmid-map-sequence-id-334669

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  6. TDP-REGv2(mScarlet-FLAG)#11 in pTwist-CMV backbone

    Plasmid
    #216155
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Very bright and decent dynamic range (>10-fold), slightly leaky. It uses a single intron. [Code 'B12']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic splice site and C-terminal FLAG tag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216155-plasmid-map-sequence-id-437763

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  8. pGTM_YR375_SUMO_Protease_001

    Plasmid
    #190063
    Purpose
    Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion
    Depositor
    Gaetano Montelione
    Article
    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi
    Insert
    ULP1_YEAST (ULP1 Budding Yeast)
    Use
    Tags
    8X His-MBP (Maltose Binding Protein)
    Expression
    Bacterial
    Mutation
    Construct contains residues 347 – 621
    Promoter
    T7 lac
    Available since
    Jan. 30, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    190063-plasmid-map-sequence-id-370664

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  9. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV2)

    Viral Prep
    #105545-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    HI
    Tags
    EGFP
    Available since
    Oct. 5, 2018
    Availability
    Academic Institutions and Nonprofits only

  19. pcDNA3.1_FASN

    Plasmid
    #107138
    Purpose
    His tagged human FASN cloned in pcDNA3.1 vector for expression in mammalian cells
    Depositor
    Brijesh Kumar Singh
    Insert
    Fatty acid synthase (FASN Human)
    Use
    Tags
    His
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107138-plasmid-map-sequence-id-423159

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  24. pAAV-syn-FLEX-splitTVA-EGFP-tTA (AAV1)

    Viral Prep
    #100798-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-syn-FLEX-splitTVA-EGFP-tTA (#100798). In addition to the viral particles, you will also receive purified pAAV-syn-FLEX-splitTVA-EGFP-tTA plasmid DNA. Helper virus for monosynaptic tracing with rabies virus; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene#100799). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ian Wickersham
    Article
    Liu et al Nature. 2017 Aug 31;548(7669):582-587. d
    Promoter
    Syn
    Tags
    EGFP (Cre-dependent)
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only

  31. GFP-itis pBE-t

    Plasmid
    #195342
    Purpose
    Base editor plasmid expressing ABE8e-NG-Cas9n under Theophylline Riboswitch and constituitively expressed gRNA targeting EGFP A110V
    Depositor
    Alexis Komor
    Article
    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris
    Inserts
    ecTadA(8e)-SpCas9-NG
    gRNA ctctacgcgggtcttgtagt
    Use
    CRISPR
    Tags
    Expression
    Mutation
    SpCas9n-NG: D10A, L1111R, D1135V, G1218R, E1219F,…
    Promoter
    Lac and ProC
    Available since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    195342-plasmid-map-sequence-id-383223

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  32. pLEX_306

    Plasmid
    #41391
    Purpose
    Constitutive lentiviral expression, SV40-puro; PGK-gateway-V5 tag
    Depositor
    David Root
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available since
    Nov. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    41391-plasmid-map-sequence-id-229869

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  36. AAVS1-tdTomato

    Plasmid
    #159275
    Purpose
    Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.
    Depositor
    Rudolf Jaenisch
    Article
    Ma et al Proc Natl Acad Sci U S A. 2018 Apr 10;11
    Insert
    tdTomato
    Use
    AAV; S1 knockin donor vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGGS
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    159275-plasmid-map-sequence-id-312908

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  37. TFORF3195

    Plasmid
    #144671
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ONECUT1 (ONECUT1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144671-plasmid-map-sequence-id-350517

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  41. pC0053-CMV-dPspCas13b-GS-ADAR2DD(E488Q)-delta-984-1090

    Plasmid
    #103869
    Purpose
    dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Inserts
    PspCas13b
    huADAR2DD (ADARB1 Human)
    Use
    CRISPR
    Tags
    GS and HIV NES
    Expression
    Mammalian
    Mutation
    Changed histidne 133 to alanine and histidine 105…
    Promoter
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103869-plasmid-map-sequence-id-199177

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  43. GoldenBraid 2.0 Kit

    Plasmid Kit
    #1000000076
    Purpose
    Modular cloning of multigenic DNA constructs in Plant Synthetic Biology applications. 95 GoldenBraid cloning compatible plasmids, containing standard basic parts and transcriptional units
    Depositor
    Diego Orzaez
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only

  44. pMKO.1 GFP

    Plasmid
    #10676
    Purpose
    Retroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.
    Depositor
    William Hahn
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    RNAi and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    10676-plasmid-map-sequence-id-139182

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  46. EGF-bio-His

    Plasmid
    #53340
    Purpose
    Expresses full-length Pro-epidermal growth factor precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    EGF (EGF Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53340-plasmid-map-sequence-id-83070

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  50. AAV-CAG-jGCaMP8m-WPRE (AAV9)

    Viral Prep
    #179255-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from AAV-CAG-jGCaMP8m-WPRE (#179255). In addition to the viral particles, you will also receive purified AAV-CAG-jGCaMP8m-WPRE plasmid DNA. CAG-driven expression of calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Promoter
    CAG
    Tags
    None
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
Showing: 5601 - 5650 of 160192 results