We narrowed to 147,257 results for: TRI

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pLVX-IRES-hygro-EGFP-DFCP1

Plasmid

#174651

Purpose

Vector for overexpression of EGFP-tagged DFCP1 with IRES for hygromycin selection.

Depositor

Lukas Kapitein

Article

Janssen et al J Cell Sci. 2021 Oct 1;134(19). pii: 271

Insert

EGFP-DFCP1 (ZFYVE1 Human)

Use

Lentiviral

Tags

EGFP

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AU1-mTOR-E2419K

Plasmid

#19994

Depositor

Fuyuhiko Tamanoi

Article

Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

mammalian target of rapamycin-E2419K (Mtor Rat)

Tags

AU1

Expression

Mammalian

Mutation

Changed Glutamine 2419 to Lysine

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pMXS-hs-3xHA-LIN-41

Plasmid

#52717

Purpose

retroviral expression of human LIN-41 / TRIM71 with 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pRS416Gal TDP43 WT YFP

Plasmid

#27447

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Tags

YFP

Expression

Yeast

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
myc-mTOR (1750-2549 aa)

Plasmid

#21747

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

mTOR (1750-2549) (Mtor Rat)

Tags

myc

Expression

Mammalian

Mutation

amino acids 1750-2549

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-fPV-GFP

Plasmid

#22914

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu parvalbumin promoter driving GFP

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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BK beta1

Plasmid

#113565

Purpose

mouse BK beta1 subunit in mammalian expression vector

Depositor

Robert Brenner

Article

Wang et al J Gen Physiol. 2006 Dec;128(6):731-44. d

Insert

Kcnmb1 (Kcnmb1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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SETD2(SET)-Cas9

Plasmid

#186700

Purpose

Plasmid encoding SETD2-Cas9 fusion under CMV promoter

Depositor

Jennifer Doudna

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

Insert

SETD2(SET)-Cas9

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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BII-TOP-tdT

Plasmid

#133395

Purpose

Piggybac vector for canonical Wnt signaling reporter

Depositor

Blair Madison

Article

Strubberg et al Stem Cell Reports. 2018 Aug 14;11(2):410

Insert

tdTomato-NLS

Expression

Mammalian

Mutation

N/A

Promoter

SuperTOPFlash

Available Since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pTn7xTS_SpyCatcher003:mNG

Plasmid

#225190

Purpose

Used for inserting SpyCatcher003 fused to mNeonGreen at the bacterial Tn7 site

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

SpyCatcher003:mNeonGreen

Tags

SpyCatcher

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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myc-mTOR (1271-2008 aa)

Plasmid

#21746

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

mTOR (1271-2008) (Mtor Rat)

Tags

myc

Expression

Mammalian

Mutation

amino acids 1271-2008

Available Since

Aug. 18, 2009

Availability

Academic Institutions and Nonprofits only
pLL3.7_hsa-miR-34a

Plasmid

#25791

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

hsa-miR-34a (TP53 Human)

Use

Lentiviral and RNAi

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only

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GST-Hsp90 C(626-732)

Plasmid

#22483

Depositor

William Sessa

Article

Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

Insert

Hsp90alpha C domain (HSP90AA2P Human)

Tags

GST

Expression

Bacterial

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pQdC12a

Plasmid

#191356

Purpose

Clostridium CRISPRi plasmid with TetR-repressible dCas12a (LbCpf1) expression

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Inserts

PGusA2-TetO2/1-dCas12a (LbCpf1)
miniPthl-tetR

Expression

Bacterial

Promoter

PGus2A-TetO2/1 and miniPthl

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-FLPo-T2A-GFP

Plasmid

#161766

Purpose

express FLPo and GFP under the control of human synapsin promoter

Depositor

Baoji Xu

Article

An et al Nat Commun. 2020 Apr 7;11(1):1729. doi:

Inserts

FLPo
T2AEGFP

Use

AAV

Expression

Mammalian

Promoter

hSynapsin1

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-SypHluorin 2x (S2x)

Plasmid

#37004

Depositor

Stephen Heinemann, Yongling Zhu

Article

Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

Insert

SypHluorin 2x (Syp Rat)

Tags

HA

Expression

Mammalian

Mutation

Two copies of pHluorin inserted at site 184-185

Promoter

CMV

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pBabe neo TGFBR3-HA

Plasmid

#83095

Purpose

Retroviral vector for constitutive TGFBR3 expression with C-terminal HA tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

TGFBR3 (TGFBR3 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
ABHD16A-mNG

Plasmid

#195157

Purpose

mammalian expression of ABHD16A tagged with mNeonGreen

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

ABHD16A (ABHD16A Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaTdT-39

Plasmid

#228980

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-39

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pREP3-TEV-NLS

Plasmid

#21146

Depositor

Stephen Kearsey

Article

Yang et al BMC Mol Biol. 2005 Jun 7;6:13. doi: 10.1

Insert

TEV protease (TEVgp1 tobacco etch virus)

Use

S. pombe

Tags

2xNLS, NLS, and myc

Expression

Yeast

Available Since

July 6, 2009

Availability

Academic Institutions and Nonprofits only

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HA.PKD.K/W

Plasmid

#10809

Depositor

Alex Toker

Article

Storz et al J Biol Chem. 2003 May 16. 278(20):17969-

Insert

PKD (PRKD1 Human)

Tags

HA and Myc (inserted between amino acids 33&3…

Expression

Mammalian

Mutation

Kinase dead mutant: K612W (Lys 612 to Trp) (bp183…

Promoter

CMV

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pMXs-hs-LIN-41

Plasmid

#52716

Purpose

retroviral expression of human LIN-41 / TRIM71

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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MSGV Mouse Acceptor PGK NGFR

Plasmid

#128482

Purpose

A TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR expressing NGFR ref Biotechniques. 2015 Mar 1;58(3):135-9

Depositor

David Ott

Article

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Promoter

MSCV

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-TMCC1

Plasmid

#120932

Purpose

Expresses human TMCC1 in mammalian cells

Depositor

Gia Voeltz

Article

Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

Insert

Trans membrane and coiled coil domain containing protein 1 (TMCC1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTn7xTS_SnoopCatcher:mNG

Plasmid

#225191

Purpose

Used for inserting SnoopCatcher fused to mNeonGreen at the bacterial Tn7 site

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

SnoopCatcher:mNeonGreen

Tags

SnoopCatcher

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS416 Gal TDP43 WT

Plasmid

#27458

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Expression

Yeast

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only
pTK47_mCherry-LGN-hardened

Plasmid

#46347

Purpose

rescues the phenotypes caused by LGN RNAi

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

LGN (GPSM2 Human)

Tags

mCherry

Expression

Mammalian

Mutation

siRNA resistant, gaGTtGacTgcTcgTTtA; deletion of …

Promoter

CMV

Available Since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-Cep83

Plasmid

#128874

Purpose

For mammalian expression of mouse Cep83

Depositor

Aimin Liu

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

Insert

Cep83 (Ccdc41 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Xl-VSP1 R152Q

Plasmid

#51881

Purpose

expression of Xl-VSP1 mutant in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP1 R152Q (tpte2 Frog)

Use

Xenopus oocyte expression

Expression

Mammalian

Mutation

R152Q: near the extracellular side of the fourth …

Promoter

CMV

Available Since

May 22, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
p5M-hPR-B

Plasmid

#89114

Purpose

mammalian expression of full-length human progesterone receptor-B in pCMV5 with 5 restriction sites deleted in polylinker

Depositor

Elizabeth Wilson

Article

Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

Insert

human progesterone receptor-B (PGR Human)

Expression

Mammalian

Mutation

5 restriction sites deleted in polylinker

Promoter

CMV

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK23

Plasmid

#37405

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

Insert

Neuromodulin (GAP43 Human)

Tags

S-tag, TEV, and mCherry

Expression

Mammalian

Mutation

Contains the first 20aa of GAP43 (neuromodulin)

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
gRNA-leu-HYB

Plasmid

#64332

Purpose

leu2 deletion gRNA cassette carried by pRS42H

Depositor

Yong-Su Jin

Article

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

gBlock product of leu2 deletion gRNA cassette

Expression

Yeast

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pTn7xTS_SpyCatcher:mKate2

Plasmid

#225188

Purpose

Used for inserting SpyCatcher fused to mKate2 at the bacterial Tn7 site

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

SpyCatcher:mKate2

Tags

SpyCatcher

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
3XFlag-TMCC1

Plasmid

#121045

Purpose

Expresses human TMCC1 in mammalian cells

Depositor

Gia Voeltz

Article

Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

Insert

Trans membrane and coiled coil domain containing protein 1 (TMCC1 Human)

Tags

3x-Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCA24N-ligase-cTF

Plasmid

#87743

Purpose

IPTG-inducible expression of ligase-cTF fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Tags

6xHis and cTF (chimeric transcription factor base…

Expression

Bacterial

Promoter

T5-lac

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MC177VSG221RNAi

Plasmid

#59730

Purpose

For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

Depositor

Gloria Rudenko

Article

Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

Insert

VSG221 RNAi fragment

Use

RNAi

Promoter

opposing T7 promoters

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV4a-SIRT6-H133Y-FLAG

Plasmid

#102324

Purpose

Human SIRT6 H133Y with FLAG tag for mammalian expression

Depositor

Hening Lin

Article

Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

Insert

SIRT6 (SIRT6 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed histidine 133 to tyrosine

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS2387-Tier1-PhCMV-p2a_mTagBFP2

Plasmid

#169524

Purpose

Tier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven P2A-fused monomeric Tag blue fluorescent protein 2

Expression

Mammalian

Promoter

PhCMV

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1-HsRCK_E

Plasmid

#146210

Purpose

Bacterial Expression of HsRck

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

Insert

HsRck (DDX6 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
gRNA-his-HYB

Plasmid

#64333

Purpose

his3 deletion gRNA cassette carried by pRS42H

Depositor

Yong-Su Jin

Article

Zhang et al Appl Environ Microbiol. 2014 Dec;80(24):

Insert

gBlock product of his3 deletion gRNA cassette

Expression

Yeast

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
L2_UBE2:eG-Lt-CsA

Plasmid

#136134

Purpose

Plasmid to transform Mp with constitutive promoter marking cell outline with eGFP. HygR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-35S:HygR p5-MpUBE2:eGFP-Lti6b

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTS2395-Tier1-PhCMV-Igk_mRuby2_KDEL

Plasmid

#169533

Purpose

Tier-1 vector encoding PhCMV-driven red fluorescent protein mRuby2 localized to the ER (PhCMV-Igk-mRuby2-KDEL-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven ER-localized monomeric red fluorescent protein mRuby2

Expression

Mammalian

Promoter

PhCMV

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-AU1-mTOR-L1460P

Plasmid

#19995

Depositor

Fuyuhiko Tamanoi

Article

Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

mammalian target of rapamycin-L1460P (Mtor Rat)

Tags

AU1

Expression

Mammalian

Mutation

Changed Leucine 1460 to Proline

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pJB38-NWMN2930

Plasmid

#84457

Purpose

pJB38 plasmid containing the S.aureus Newman genetic region between genes 29 and 30

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

Staphylococcus aureus NWMN genomic region NWMN29-30

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-GST-2

Plasmid

#228984

Purpose

For bacterial expression of anti-GST nanobody GST-2, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-2

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-GST-1

Plasmid

#228983

Purpose

For bacterial expression of anti-GST nanobody GST-1, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-1

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
ECFP(W66A)-FRB-MoA

Plasmid

#67904

Purpose

Expresses a Mitochondria located FRB domain in mammalian cells (the ECFP is not fluorescent)

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

ECFP(W66A)-FRB-MoA

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-FLAG-Bbs4

Plasmid

#128868

Purpose

For mammalian expression of mouse Bbs4

Depositor

Aimin Liu

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

Insert

Bbs4 (Bbs4 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
myc-BioID-Rab7

Plasmid

#121049

Purpose

Expresses myc tagged BioID and human Rab7a in mammalian cells

Depositor

Gia Voeltz

Article

Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

Inserts

BioID
Rab7A

Tags

myc

Expression

Mammalian

Mutation

R118G (highly promiscuous form)

Promoter

CMV

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-TMCC1

Plasmid

#120931

Purpose

Expresses human TMCC1 in mammalian cells

Depositor

Gia Voeltz

Article

Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

Insert

Trans membrane and coiled coil domain containing protein 1 (TMCC1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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