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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 66,850 results for: nin

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Showing: 241 - 260 of 66850 results

  4. Inducible Caspex expression

    Plasmid
    #97421
    Purpose
    For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics
    Depositor
    Steven Carr, Samuel Myers
    Publication
    Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Caspex
    T2A-Gfp
    Use
    CRISPR
    Tags
    3xFLAG and V5
    Expression
    Mammalian
    Mutation
    dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …
    Promoter
    TRE3G
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    97421-plasmid-map-sequence-id-466895

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  5. pCRIS-PITChv2-dTAG-Puro (BRD4)

    Plasmid
    #91796
    Purpose
    PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.
    Depositor
    James Bradner, Behnam Nabet
    Publication
    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FKBP_F36V-2xHA-P2A-Puro
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Phenylalanine 36 to valine in FKBP12
    Promoter
    Promotorless
    Available since
    March 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    91796-plasmid-map-sequence-id-334366

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  10. pB-CAGGS-dCas9-KRAB-MeCP2

    Plasmid
    #110824
    Purpose
    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2
    Depositor
    Alejandro Chavez, George Church
    Publication
    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-KRAB-MeCP2
    Expression
    Mammalian
    Mutation
    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…
    Promoter
    CAGGS
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    110824-plasmid-map-sequence-id-324008

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  12. pLV-dCas9-p300-P2A-PuroR

    Plasmid
    #83889
    Purpose
    Lentiviral Sp dCas9-p300-P2A-PuroR
    Depositor
    Charles Gersbach
    Publication
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    pac from Streptomyces alboniger
    Use
    CRISPR and Lentiviral
    Tags
    Flag and nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83889-plasmid-map-sequence-id-297764

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  14. pCMV-PEmax-P2A-GFP

    Plasmid
    #180020
    Purpose
    Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PEmax-P2A-EGFP
    Tags
    P2A-EGFP, SV40 bpNLS, and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    CMV
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    180020-plasmid-map-sequence-id-352752

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  16. LSL-Cas9-Rosa26TV

    Plasmid
    #61408
    Purpose
    Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    EGFP
    Use
    CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    3xFLAG and P2A
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    CAG
    Available since
    Jan. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61408-plasmid-map-sequence-id-333613

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Showing: 241 - 260 of 66850 results