We narrowed to 11,219 results for: NSI
-
-
pFUGW-ZipACR-EYFP
Plasmid#88844PurposeFast anion channelrhodopsin (current half-decay time 2-4 ms depending on voltage) for time-resolved inhibition of neuronal spikingDepositorInsertZipACR-NotI-EYFP
UseLentiviralTagsEYFPExpressionMutationPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
H2B-mScarlet- hRec8(297-506)-mNeonGreen
Plasmid#174717PurposeStable expression of fluorescent Separase cleavage sensor with human Rec8DepositorInsertRec8 (REC8 Human)
UseRetroviralTagsH2B-mScarlet and mNeonGreenExpressionMammalianMutation297-506PromoterAvailable sinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-FRB:Akt2
Plasmid#72899PurposeRecruitable Akt2 (LocaTOR2)DepositorInsertSerine/threonine protein kinase Bβ (AKT2 Human)
UseLentiviralTagsFRB(T2098L)ExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
TDP43 NOTAG6
Plasmid#28209DepositorInsertTar DNA binding protein (TARDBP Human)
UseTagsExpressionMammalianMutationaa1-314 onlyPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
GW1-mito-mCherryEA
Plasmid#207868PurposeMitochondria-targeted mCherry(I158E/Q160A) mutant as a red fluorescent pH sensorDepositorInsertmito-mCherryEA
UseTags4xCox8 mitochondrial targetingExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-Myo 1d
Plasmid#134906PurposeExpression of rat Myo 1d (Myr 4) in mammalian cells.DepositorInsertMyo 1d (rat) with 3' UTR (Myo1d Rat)
UseTagsEGFPExpressionMammalianMutationPromoterCMV IEAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-CenpB 1-166
Plasmid#108475Purposeexpression of GFP-CenpB 1-166DepositorInsertCenpB 1-166 AA (CENPB Human)
UseTagsEGFPExpressionMammalianMutationencodes only the DNA-binding domain of CENP-BPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_RGG-RFP-RGG
Plasmid#124934PurposeExpression of RGG-RFP-RGGDepositorInsertRGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-ExRai-AKAR-NES
Plasmid#118407PurposeCytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.DepositorInsertExRai-AKAR-NES
UseTagsNuclear export signal (NES)ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SNX18-1/1
Plasmid#91369PurposeProtein expression and purification of human SH3 domain construct SNX18-1/1DepositorInsertSNX18-1/1 (SNX18 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_TNK2-1/1
Plasmid#91377PurposeProtein expression and purification of human SH3 domain construct TNK2-1/1DepositorInsertTNK2-1/1 (TNK2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109AsAmp30E
Plasmid#123144PurposeArsenic sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, ParsR, PhrpLEAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
IFI16-(192-393)-HinA (pcDNA3-FLAG)
Plasmid#35062DepositorInsertIFI16-(192-393)-HinA (IFI16 Human)
UseTagsFLAG and HisExpressionMammalianMutationContains HinA domain: aa 192-393PromoterCMVAvailable sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
RIPR-bio-His
Plasmid#50824PurposeExpresses enzymatically monobiotinylated full length RIPRDepositorInsertRIPR
UseTagsratCD4d3+4,biotinylation sequence, 6xHisExpressionMammalianMutationall potential N-linked glycosylation sites (N-X-S…PromoterCMVAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
trPAGmEos3.2
Plasmid#203752PurposeEncodes the transmembrane domain from PAG/CSK, including its 2 palmitoylation sites, with mEos3.2 fused on the C terminus. Used as a fluorescent plasma membrane probe.DepositorInserttrPAG (PAG1 Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_TGON2
Plasmid#111677PurposeMAC-tagged gene expressionDepositorInsertTGON2 (TGOLN2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
IFI16-(515-710)-HinB (pcDNA3-FLAG)
Plasmid#35063DepositorInsertIFI16-(515-710)-HinB (IFI16 Human)
UseTagsFLAGExpressionMammalianMutationContains HinB domain: aa 515-710PromoterCMVAvailable sinceApril 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-36β
Plasmid#214344PurposeProtein overexpression in bacteriaDepositorInsertIL36B (IL36B Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
EK0423 SFFV-mTagBFP2-SGc(s70587)cSG-mCherry (FLP-IN)
Plasmid#191162PurposeExpression of a sensor for synthetic sequence 1 (EK0208) in mammalian cells; mTagBFP2 marker, mCherry outputDepositorInsertmTagBFP:s70587:mCherry
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHES941
Plasmid#87942PurposeConstitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Gal4 binding sites.DepositorInsertGAL4 DBD - hPR LBD - MSN2 AD (PGR Human, Synthetic, Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-IP2.0
Plasmid#109217PurposeExpress IP2.0 in E. coliDepositorInsertIP2.0 (Inverse Pericam 2.0)
UseTagsHis-tagExpressionBacterialMutationPromoterAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB088i
Plasmid#79216PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-TnCAD-F3
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1); Ppro1Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
As2
Plasmid#123160PurposeArsenic sensor used in the sensor cell array. pSB4A3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, ParsR-ABS84, PhrpLEAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
HM3C NaVAe1_p pET24b
Plasmid#127854PurposeBacterial expression vector for NaVAe1 bacterial sodium channel residues 143-288. It expresses HIStag-MBP-3C-channel protein.DepositorInsertNaVAe1p
UseTags3C Cleavage site, His, and MBPExpressionBacterialMutationPromoterAvailable sinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAB085c
Plasmid#79208PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-FL
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1); PtetAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pACE-GIRK4-S
Plasmid#172425PurposeExpression of full-length, human GIRK4 with C-terminal StrepTag IIDepositorInsertGIRK4 (KCNJ5 Human)
UseTagsStreptag IIExpressionInsectMutationPromoterAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal TDP43 WT
Plasmid#27458DepositorInsertTDP-43 (TARDBP Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGL-p21UTR
Plasmid#20014DepositorInsertp21 3'UTR (Cdkn1a Mouse)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
uORF1-Mreinit- 36xMashTag-BFP-3xStop-24xPP7 (uORF-MashTag reporter)
Plasmid#128609PurposeExpresses uORF1 -36xMashTag with MoonTag as readout for reinitiation, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.DepositorInsertuORF1-36xMashTag
UseTagsExpressionMammalianMutationPromoterPcmvAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RUSC1-1/1
Plasmid#91379PurposeProtein expression and purification of human SH3 domain construct RUSC1-1/1DepositorInsertRUSC1-1/1 (RUSC1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-sapphireAKAR-NLS
Plasmid#118467Purposecp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.DepositorInsertsapphireAKAR-NLS
UseTagsNuclear localization signal (NLS)ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
N1-mSin1.5-GFP
Plasmid#72909PurposeGFP-tagged mSin1 (isoform 5)DepositorInsertTarget of rapamycin complex 2 subunit MAPKAP1, isoform 5 (MAPKAP1 Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109HgAmp30E
Plasmid#123149PurposeMercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ109, PmerT, PhrpLEAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-KRaION1
Plasmid#177799PurposeBacterial expression of green fluorescent potassium indicator KRaION1DepositorInsertKRaION1
UseTagsExpressionBacterialMutationPromoteraraBAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-UTRN-mini
Plasmid#158749PurposeExpresses a shorter version of Utrophin (amino acid 35-136) with amino terminus eGFP in mammalian cellsDepositorInsertUTRN-mini
UseTagseGFPExpressionMammalianMutationPromoterCMV IE94Available sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 RL3Sc
Plasmid#109365PurposeHuman rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tagDepositorInsertUseTags1D4ExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKD280.7
Plasmid#173172PurposeExpresses SO_4387DepositorInsertSO_4387
UseTagsExpressionBacterialMutationPromoterJ23107Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
EK0325 SFFV-mCherry-SG(EGFP.s2)SG-Cre (FLP-IN)
Plasmid#191160PurposeExpression of a sensor for EGFP CDS in mammalian cells; mCherry marker, Cre outputDepositorInsertmCherry:EGFP.s2:Cre
UseTagsExpressionMammalianMutationWTPromoterSFFVAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only
