We narrowed to 24,878 results for: Spr

1. Lenti-Cas9-gRNA-TagBFP2

   Plasmid

   #124774

   Purpose

   3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-Cas9-2A-GFP-sgRNA

   Plasmid

   #124889

   Purpose

   Contains spCas9-2A-GFP and guide RNA scaffold in single retroviral vector

   Depositor

   Harvey Lodish

   Article

   Li et al Haematologica. 2016 Jun;101(6):e216-9. d

   Insert

   Cas9
   

   Use

   Retroviral

   Tags

   2A-GFP

   Promoter

   MSCV

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpCas9 (PX165)

   Plasmid

   #48137

   Purpose

   Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti_gRNA-Puro

   Plasmid

   #84752

   Purpose

   Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV hUbC-dCas9 KRAB-T2A-GFP

   Plasmid

   #67620

   Purpose

   Co-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized dead Cas9 KRAB T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Mutation

   D10A and H840A

   Available Since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJH001

   Plasmid

   #67641

   Purpose

   High copy Cas9 expression vector with LEU2 marker for yeast transformation

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Inserts

   Cas9
   Empty
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCI108-SiT-Cas12a-[Cond]

   Plasmid

   #128407

   Purpose

   Conditionally express both AsCas12a and CRISPR arrays on single transcripts

   Depositor

   Randall Platt

   Article

   Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

   Insert

   AsCas12a-Triplex
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR_TRE3G-p300-dCas9-P2A-mCherry

   Plasmid

   #138456

   Purpose

   2nd Generation Lentiviral vector. Expresses an N-terminal p300-dCas9 fusion protein and mCherry

   Depositor

   Jian Xu

   Article

   Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

   Insert

   p300 core
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330A-FBL/PITCh

   Plasmid

   #63671

   Purpose

   Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pHR_TRE3G-LSD1-dCas9-P2A-mCherry

    Plasmid

    #138462

    Purpose

    2nd Generation Lentiviral vector. Expresses an N-terminal LSD1-dCas9 fusion protein and mCherry

    Depositor

    Jian Xu

    Article

    Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

    Insert

    LSD1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP

    Plasmid

    #50946

    Purpose

    An empty gRNA expression vector

    Depositor

    Kosuke Yusa

    Article

    Koike-Yusa et al Nat Biotechnol. 2014 Mar;32(3):267-73. d

    Insert

    None
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. p414-TEF1p-Cas9-CYC1t

    Plasmid

    #43802

    Purpose

    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    Human Optimized S. pyogenes Cas9
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    TEF1 promoter

    Available Since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS SK mCherryROSAegfp

    Plasmid

    #54322

    Purpose

    Encodes mCherry fused with an out-of-frame EGFP through a linker containing a target for TALEN/CRISPR. Can be used as a surrogate target to test the specificity of TALENs and CRISPR.

    Depositor

    Silvestro Conticello

    Article

    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

    Insert

    mCherry and EGFP
    

    Use

    CRISPR, Mouse Targeting, and TALEN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. p415-GalL-Cas9-CYC1t

    Plasmid

    #43804

    Purpose

    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    Human Optimized S. pyogenes Cas9
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    GalL

    Available Since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCasAb-apr

    Plasmid

    #121998

    Purpose

    Bacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumannii

    Depositor

    Quanjiang Ji

    Article

    Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLSC-5

    Plasmid

    #62889

    Purpose

    Lenti vector for expression of inducible split Cas9

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Inserts

    SpCas9(574-1368)
    SpCas9(2-573)
    

    Use

    CRISPR and Lentiviral

    Tags

    FFBP12, FRB, NES PTK2, NLS SV40, and P2A

    Expression

    Mammalian

    Mutation

    *see comment below

    Promoter

    EFS

    Available Since

    March 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC0052-REPAIR non-targeting guide clone into pC0043

    Plasmid

    #103868

    Purpose

    Non-targeting guide RNA for use with PspCas13b. Useful as a control for RNA editing and RNA knockdown experiments.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    non-targeting guide RNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBECAb-apr

    Plasmid

    #122001

    Purpose

    A cytidine deaminase-mediated base-editing plasmid in Acinetobacter baumannii

    Depositor

    Quanjiang Ji

    Article

    Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRU294

    Plasmid

    #167689

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::Cas9i
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSQT1601

    Plasmid

    #53369

    Purpose

    human codon optimized Csy4 and FokI-dCas9 expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    hCsy4-T2A-NLS-hFokI-dCas9-NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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