We narrowed to 10,326 results for: ada
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Plasmid#85444PurposeExpresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR
UseMinimal backbone fragment for low-copy bacterial …ExpressionMammalianAvailable SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNH605-PACE-YFP
Plasmid#172002PurposeFluorescent reporter for Rpn4 activityDepositorInsert4xPACE-YFP
UseVector for genomic integration into the yeast gen…ExpressionYeastPromoterCYC1 core promotor with 4 tandem repeats of Rpn4 …Available SinceNov. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28aAviHisCD72cCTLD
Plasmid#129066Purposegeneration of biotinylated mouse CD72c CTLD protein using bacteriaDepositorInsertCD72c (Cd72 Mouse)
ExpressionBacterialAvailable SinceMay 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
px458_2A_GFP_sgRNA_G3BP1_G1
Plasmid#127114DepositorInsertgRNA G3BP1 (G3BP1 Human)
UseCRISPRAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT2 Beta1b GFP
Plasmid#89893PurposeMammalian expression of Beta1b GFPDepositorInsertCacnb1 (Cacnb1 Rat)
TagsGFPExpressionMammalianMutationGFP sequence inserted just before the Stop codonPromoteradenovirus major late promoterAvailable SinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330-RAB7A
Plasmid#227297PurposeExpresses SpCas9 and a sgRNA targeting the N-terminus of RAB7A for knock-in.DepositorInsertsgRNA Targeting N-terminus of RAB7A (RAB7A Human)
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Zeo-H2BC11
Plasmid#207759PurposeDonor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorInsertH2BC11 Homology Arms flanking a moxGFP-Zeo Cassette (H2BC11 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMT2 Beta1b mCherry
Plasmid#89892PurposeMammalian expression of Beta1b mCherryDepositorInsertCacnb1 (Cacnb1 Rat)
TagsmCherryExpressionMammalianMutationmCherry sequence inserted just before the Stop co…Promoteradenovirus major late promoterAvailable SinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMonAID_nCas9-PmCDA_Hyg_ALS
Plasmid#91693PurposeMonocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAlsDepositorInsertSpCas9
TagsPmCDA1ExpressionPlantMutationD10A for nickase Cas9Promoter2x35SAvailable SinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
Mini-Ii-Str_SBP-EGFP-LIMP2
Plasmid#222324PurposeSynchronize the trafficking of LIMP2 from the ER.DepositorInsertStreptavidin-KDEL and LIMP2 fused to SBP-EGFP (SCARB2 Human)
ExpressionMammalianPromoterCMVAvailable SinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-APOBEC1-UdgX-nCas9
Plasmid#163555PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-APOBEC1-UdgX-nCas9
UseCRISPRExpressionMammalianMutationnCas9 (D10A)Available SinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
aav-tnt-actn2-gfp
Plasmid#165034PurposeAAV-based delivery of ACTN2-GFP into cardiomyocytes in vivoDepositorAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-APOBEC1-UdgX-HF-nCas9
Plasmid#163559PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-APOBEC1-UdgX-HF-nCas9
UseCRISPRExpressionMammalianMutationnCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)Available SinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262B-ABE8e
Plasmid#161524PurposeGateway entry clone (attL1 & attR5) for CRISPR-zSpRYCas9 ABE8e mediated A-G base editingDepositorInsertTadA8e-zSpRYCas9(D10A)
UseCRISPR; Gateway compatible tada8e-zsprycas9(d10a)…ExpressionPlantAvailable SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO5d.SFFV.eGFP-miR30n
Plasmid#90333PurposemiR30 based shMir knockdownDepositorInsertseGFP
na
SFFV
miR30n
UseLentiviralAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcsII-pb2-flag
Plasmid#86766PurposeC-terminal flag-tagged protein expression in mammalian cells, lentiviral vectorDepositorInsertproteasome beta subunit 2 (PSMB2 Human)
UseLentiviralTagsflagExpressionMammalianPromoterEF-1aAvailable SinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_RRM2
Plasmid#157866Purposeexpress His tagged hnRNPA2 RRM2DepositorAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
XIAP (pBS0852)
Plasmid#185282PurposeFor the mammalian expression of the human protein XIAP. This is the positive control we used in our apoptosis assay.DepositorInsertXIAP (XIAP Human)
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
scFv-GCN4-DNMT3a-DNMT3l
Plasmid#154140PurposeExpresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.DepositorInsertscFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
TagsHA and sfGFPExpressionMammalianPromoterSFFVAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNAD1
Plasmid#154347PurposepUC19 backbone with editing template for homologous recombination mediated deletion of Nitrate reductase gene of N. oceanica and selection based on zeocin resistanceDepositorInsertShBle
UseMicroalgaeExpressionBacterialMutationnonePromoterViolaxanthin Chlorophyll a Binding Protein Precur…Available SinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mRuby3-6xFLAG
Plasmid#161613PurposeCell morphology marker construct. Contains a red fluorescent protein mRuby3 and six FLAG tags.DepositorInsertmRuby3-6xFLAG
UseAAVExpressionMammalianMutationN/APromoterHuman synapsin 1 promoterAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Raichu-OsRac1 WT
Plasmid#133262PurposeBiosensor for monitoring activation for rice small GTPase OsRac1DepositorAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-flex-Clover
Plasmid#135177PurposeAAV expression of TRE(TRE3G)-driven, Cre-dependent, Clover expression for fluorescent lablingDepositorInsertsClover
TRE3G
UseAAVExpressionMammalianMutationa variant of GFPPromoterTRE3GAvailable SinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSW646 - pML1>>
Plasmid#115995Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpML1:B-dummy:GR-LhG4:D-dummy:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
TagsHDEL and SP(ER)ExpressionPlantAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Fireworks GFP[PTC+] (AVA2600)
Plasmid#85446PurposeExpresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
UseMinimal backbone fragment for low-copy bacterial …ExpressionMammalianAvailable SinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
Circular 200,100 with interspersed loops (FANCC)
Plasmid#180192PurposeAAV vector carrying a guide RNA targeting the human FANCC mRNADepositorInsertCircular 200,100 guide RNA with interspersed loops
UseAAVExpressionMammalianPromoterHuman U6Available SinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
Circular 200,100 with interspersed loops (CTNNB1)
Plasmid#180193PurposeAAV vector carrying a guide RNA targeting the human CTNNB1 mRNADepositorInsertCircular 200,100 guide RNA with interspersed loops
UseAAVExpressionMammalianPromoterHuman U6Available SinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV.CBA.YFP.miR-E_shControl
Plasmid#180386PurposeProducing AAV that encodes negative control shRNA with miR-E backboneDepositorInsertshControl
UseAAV and RNAiPromoterCBAAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDicAID_nCas9-PmCDA-2A-NptII_ETR
Plasmid#91695PurposeDicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1-2A-NptII with sgRNA targeting SlEtrDepositorInsertSpCas9
TagsPmCDA1ExpressionPlantMutationD10A for nickase Cas9PromoterPcUbiAvailable SinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_RRM1
Plasmid#157865Purposeexpress His tagged hnRNPA2 RRM1DepositorAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIYN3
Plasmid#197099PurposeTo express the variant of M. jannaschii TyrRS specific for halogenated tyrosines and Nap3 amber suppressor tRNA and allow UAG codon to be translated into these derivatives.DepositorInsertTyrRS variant, Nap3 tRNA
ExpressionBacterialMutationAla70, Thr158, Ser159, Arg286 (TyrRS)Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-ChroME2f-ST-P2A-NLS-mRuby3
Plasmid#170175PurposeCation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vectorDepositorInsertChroME2f-ST-P2A-NLS-mRuby3
UseAAVExpressionMammalianAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET48 AtGUN1-PS
Plasmid#136358PurposeBacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 918)DepositorInsertcDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 918 (GUN1 Mustard Weed)
TagsTRX-HisExpressionBacterialPromoterT7Available SinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-VCPIP1-Strep-HA
Plasmid#113482PurposeExpression of human VCPIP1 with C-terminal strep-HA tagDepositorInsertVCPIP1 (VCPIP1 Human)
Tags2xstrep-HAExpressionMammalianMutationwildtype without stop codonPromoterCMVAvailable SinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MCS-eDHFR(69K6)-EGFP
Plasmid#172101PurposeMammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFPDepositorInsert22aa-eDHFR(69K6)-EGFP
ExpressionMammalianMutationeDHFR(69K6): Hexalysine (K6) sequence is inserted…PromoterCMVAvailable SinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAY19 (OsTIR1(F74A) mAID-EGFP-NLS)
Plasmid#140535PurposeOsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2DepositorInsertmAID-EGFP-NLS TOL2
ExpressionMammalianAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-PRAS40 WT
Plasmid#86758PurposeN-terminal HA-tagged protein expression in mammalian cellsDepositorAvailable SinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-FNTA-FLAG
Plasmid#86858PurposeTo express FNTA with a FLAG tag at C terminus.DepositorInsertFNTA (FNTA Human)
TagsFLAGExpressionMammalianMutationThe sequence of the insert is isoform 2, which di…PromoterCMVAvailable SinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2R3a-3AT
Plasmid#71273PurposeEntry clone containing 3AT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertT3A ribulose-1,5-bisphosphate carboxylase 3A subunit terminator
UseGatewayAvailable SinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-MYO1C
Plasmid#227306PurposeDonor template for mStayGold insertion into the N-terminus of the MYO1C locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MYO1C (Addgene #227305)DepositorInsertMYO1C Homology Arms flanking a mStayGold Tag (MYO1C Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only