Addgene

We narrowed to 9,690 results for: ada

Showing: 5601 - 5640 of 9690 results

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pET28a-DONSON-Δ24

Plasmid

#208801

Purpose

Xenopus laevis His6-Myc-DONSON (1-24aa removed)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

Δ24, deletion of 1-24aa

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-DONSON-DEW

Plasmid

#208802

Purpose

Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A)

Depositor

Aga Gambus

Article

Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

Insert

DONSON (donson.L Frog)

Use

Tags

His and Myc

Expression

Bacterial

Mutation

D374A; E377A; W381A

Promoter

T7

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Solo-sTagRFP-TUBA1B

Plasmid

#207765

Purpose

Donor template for sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

TUBA1B Homology Arms flanking a sTagRFP Tag (TUBA1B Human)

Use

CRISPR; Donor template

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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GFP Cav2.2 W391A pRK5

Plasmid

#206084

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

Depositor

Annette Dolphin

Article

Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

GFP

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pHD22y/PfPRMT5-PTP

Plasmid

#203160

Purpose

for tagging PTP to the 5' end of PfPRMT5 gene by single crossover

Depositor

Liwang Cui

Article

Lucky et al Commun Biol. 2023 Jun 22;6(1):659. doi:

Insert

PfPRMT5 fragment, PTP tag and pDT 3' UTR

Use

Tags

3' UTR of P. berghei dihydrofolate reductase…

Expression

Mammalian

Mutation

Promoter

No

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA W391A pcDNA3

Plasmid

#206071

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2

Depositor

Annette Dolphin

Article

Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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iCAP-BI-49-KanR

Plasmid

#203534

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

Insert

AAV-BI49 Cap

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMRX-IRES-puro-3xFLAG

Plasmid

#204545

Purpose

Expresses 3xFLAG tag in mammalian cells

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

3xFlag

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1-MVP

Plasmid

#204541

Purpose

Expresses GST-tagged MVP in E.coli

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

MVP (MVP Human)

Use

Tags

Expression

Bacterial

Mutation

None

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1_AdoLightG

Plasmid

#187465

Purpose

Expresses green adenosine indicator AdoLightG in neuronal cells

Depositor

Tommaso Patriarchi

Article

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Insert

AdoLightG

Use

AAV

Tags

Flag tag

Expression

Mutation

Promoter

human Synapsin-1

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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iCAP-BI-65-KanR

Plasmid

#203536

Purpose

RepCap for AAV production

Depositor

Ben Deverman

Article

Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

Insert

AAV-BI65 Cap

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pSC5-toxic3

Plasmid

#188625

Purpose

pSC5GGv2 with HindII Gene Insert

Depositor

Bogumil Karas

Article

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Insert

HindII

Use

Synthetic Biology; Diatom expression

Tags

Expression

Bacterial and Yeast

Mutation

ACT1 intron insert

Promoter

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pNIC28-HiBIT-WDR5

Plasmid

#200883

Purpose

Bacterial expression of N-terminal HiBIT-tagged human WDR5 protein

Depositor

Stefan Knapp

Article

Schwalm et al Cell Chem Biol. 2023 Jun 20:S2451-9456(2

Insert

HiBIT-WDR5 (WDR5 Human)

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-mut4/1_2_VB

Plasmid

#186444

Purpose

Encodes Cas4(K81A)/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1 and Cas2

Use

CRISPR

Tags

Expression

Mutation

Cas4 domain (K81A)

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsEDC4_532-979_V

Plasmid

#147783

Purpose

Mammalian Expression of HsEDC4_532-979

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

HsEDC4_532-979 (EDC4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsEDC4_974-1265_V

Plasmid

#147784

Purpose

Mammalian Expression of HsEDC4_974-1265

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

HsEDC4_974-1265 (EDC4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsEDC4_1266-1401_V

Plasmid

#147785

Purpose

Mammalian Expression of HsEDC4_1266-1401

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

HsEDC4_1266-1401 (EDC4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsTNRC6A-M2WA-CIM1-2mut-siRNAres_S

Plasmid

#147517

Purpose

Insect Expression of HsTNRC6A-M2WA-CIM1-2mut-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A-M2WA-CIM1-2mut-siRNAres (TNRC6A Human)

Use

Tags

Expression

Insect

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsTNRC6A-M2WA-F1359A-siRNAres_S

Plasmid

#147518

Purpose

Mammalian Expression of HsTNRC6A-M2WA-F1359A-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A-M2WA-F1359A-siRNAres (TNRC6A Human)

Use

Tags

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1-HsTNRC6A_1209-1709-CIM1-2mut_S

Plasmid

#147521

Purpose

Bacterial Expression of HsTNRC6A_1209-1709-CIM1-2mut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A_1209-1709-CIM1-2mut (TNRC6A Human)

Use

Tags

Expression

Bacterial

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1-HsTNRC6A_1209-1709-CIM1-2mut-F1359A_S

Plasmid

#147522

Purpose

Bacterial Expression of HsTNRC6A_1209-1709-CIM1-2mut-F1359A

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A_1209-1709-CIM1-2mut-F1359A (TNRC6A Human)

Use

Tags

Expression

Bacterial

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1-HsTNRC6A_1209-1709-M2WA-CIM1-2mut_S

Plasmid

#147527

Purpose

Bacterial Expression of HsTNRC6A_1209-1709-M2W1-CIM1-2mut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A_1209-1709-M2W1-CIM1-2mut (TNRC6A Human)

Use

Tags

Expression

Bacterial

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-6P-1-HsTNRC6A_1209-1709-M2WA-F1359A_S

Plasmid

#147528

Purpose

Bacterial Expression of HsTNRC6A_1209-1709-M2W1-F1359A

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A_1209-1709-M2W1-F1359A (TNRC6A Human)

Use

Tags

Expression

Bacterial

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsTNRC6A-CIM1-2mut-siRNAres_R

Plasmid

#147412

Purpose

Insect Expression of HsTNRC6A-CIM1-2mut-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A-CIM1-2mut-siRNAres (TNRC6A Human)

Use

Tags

Expression

Insect

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690_R

Plasmid

#147393

Purpose

Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-HsTNRC6C_1207-1690 (TNRC6C Nematode, Human)

Use

Tags

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1mut_R

Plasmid

#147395

Purpose

Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1mut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1mut (TNRC6C Nematode, Human)

Use

Tags

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-F1389A_R

Plasmid

#147398

Purpose

Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-F1389A

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-HsTNRC6C_1207-1690-F1389A (TNRC6C Nematode, Human)

Use

Tags

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-P-GL-mut_R

Plasmid

#147399

Purpose

Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-P-GL-mut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-HsTNRC6C_1207-1690-P-GL-mut (TNRC6C Nematode, Human)

Use

Tags

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCIneo-lambdaN-HA-HsTNRC6A-CIM1-2mut-F1359A-siRNAres_R

Plasmid

#147411

Purpose

Insect Expression of HsTNRC6A-CIM1-2mut-F1359A-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A-CIM1-2mut-F1359A-siRNAres (TNRC6A Human)

Use

Tags

Expression

Insect

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-F961A_R

Plasmid

#147386

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384-F961A

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384-F961A (gw Fly, Nematode)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM-60-HsNot3_656-753_P

Plasmid

#147223

Purpose

Bacterial Expression of HsNot3_656-753

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

HsNot3_656-753 (CNOT3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpHM-HsNot2_429-527_P

Plasmid

#147224

Purpose

Bacterial Expression of HsNot2_429-527

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

HsNot2_429-527 (CNOT2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pnYC-NpHM-HsNot2_429-540_P

Plasmid

#147225

Purpose

Bacterial Expression of HsNot2_429-540

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

HsNot2_429-540 (CNOT2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1A-DmXRN1-Q1346AQ1350A-dsRNAres-V5His6_P

Plasmid

#147211

Purpose

Insect Expression of DmXRN1-Q1346AQ1350A-dsRNAres

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1-Q1346AQ1350A-dsRNAres (pcm Fly)

Use

Tags

Expression

Insect

Mutation

one silent mtuation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1A-DmXRN1-Q1346P-dsRNAres-V5His6_P

Plasmid

#147212

Purpose

Insect Expression of DmXRN1-Q1346P-dsRNAres

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1-Q1346P-dsRNAres (pcm Fly)

Use

Tags

Expression

Insect

Mutation

one silent mtuation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1A-DmXRN1-W1345A-dsRNAres-V5His6_P

Plasmid

#147213

Purpose

Insect Expression of DmXRN1-W1345A-dsRNAres

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1-W1345A-dsRNAres (pcm Fly)

Use

Tags

Expression

Insect

Mutation

one silent mtuation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmGW182_539-830_O

Plasmid

#147150

Purpose

Insect Expression of DmGW182_539-830

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182_539-830 (gw Fly)

Use

Tags

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmGW182_539-1115_O

Plasmid

#147151

Purpose

Insect Expression of DmGW182_539-1115

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182_539-1115 (gw Fly)

Use

Tags

Expression

Insect

Mutation

two non silent mutations V704A and N799S, and one…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmGW182-SD6Wmut_O

Plasmid

#147155

Purpose

Insect Expression of DmGW182-SD6Wmut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182-SD6Wmut (gw Fly)

Use

Tags

Expression

Insect

Mutation

Two non silent mutations V704A and N799S and one …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1A-DmXRN1_3xPmut-dsRNAres-V5His6_P

Plasmid

#147205

Purpose

Insect Expression of DmXRN1-3xPmut-dsRNAres

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1-3xPmut-dsRNAres (pcm Fly)

Use

Tags

Expression

Insect

Mutation

one silent mtuation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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