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173,387 results

Showing: 5641 - 5680 of 173387 results

  2. pZac2.1-GfaABC1D-lck-jGCaMP8m (AAV9)

    Viral Prep
    #176760-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pZac2.1-GfaABC1D-lck-jGCaMP8m (#176760). In addition to the viral particles, you will also receive purified pZac2.1-GfaABC1D-lck-jGCaMP8m plasmid DNA. GfaABC1D-driven expression of membrane-bound calcium sensor GCaMP8m. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Loren Looger
    Promoter
    GfaABC1D
    Available Since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only

  3. pSIMcpf1

    Plasmid
    #153034
    Purpose
    For CRISPR-Cas12a genome editing in E. coli. Contains; constituitively expressed Ascpf1 gene, a heat-inducible lambda red recombination system, an arabinose-inducible CRISPR array targeting pMB1.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    Ascpf1
    Expression
    Bacterial
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153034-plasmid-map-sequence-id-320039

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  7. pBAD24-sfGFPx2

    Plasmid
    #51559
    Purpose
    Superfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)
    Depositor
    Sankar Adhya, Francisco Malagon
    Article
    Malagon et al RNA. 2013 Sep;19(9):1200-7. doi: 10.1261
    Insert
    superfolder GFP
    Tags
    GLESTCRHASLAVLADERRFSA and MARARA
    Expression
    Bacterial
    Mutation
    Contains additonal aa in the N and C terminus (in…
    Available Since
    March 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    51559-plasmid-map-sequence-id-78831

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  9. pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (AAV9)

    Viral Prep
    #104492-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA. Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Article
    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi
    Promoter
    Syn
    Available Since
    Aug. 6, 2018
    Availability
    Academic Institutions and Nonprofits only

  14. TFORF0006

    Plasmid
    #142526
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TULP4 (TULP4 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    142526-plasmid-map-sequence-id-323917

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  20. AiP11851-pAAV-hDLX-minBG-iCre-4X2C-WPRE3-BGHpA (Alias: CN1851) (AAV PHP.eB)

    Viral Prep
    #164450-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from AiP11851-pAAV-hDLX-minBG-iCre-4X2C-WPRE3-BGHpA (Alias: CN1851) (#164450). In addition to the viral particles, you will also receive purified AiP11851-pAAV-hDLX-minBG-iCre-4X2C-WPRE3-BGHpA (Alias: CN1851) plasmid DNA. minBetaGlobin-driven expression of Cre in forebrain GABAergic interneurons. For use in combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Allen Institute for Brain Science, Jonathan Ting
    Article
    Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)
    Promoter
    minBetaGlobin
    Available Since
    Sept. 29, 2022
    Availability
    Academic Institutions and Nonprofits only

  22. pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine (AAV5)

    Viral Prep
    #50472-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine (#50472). In addition to the viral particles, you will also receive purified pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine plasmid DNA. GFAP-driven HA-tagged rM3D(Gs) and bicistronic mCitrine expression, for chemogenetic excitation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    GFAP
    Tags
    mCitrine
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only

  27. lenti sgRNA(MS2)_puro backbone

    Plasmid
    #73795
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73795-plasmid-map-sequence-id-181202

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  28. RP4-8

    Plasmid
    #79813
    Purpose
    Modified broad host range plasmid RP4. Gentamicin resistant. Helper plasmid for conjugation
    Depositor
    Michael Hynes
    Article
    Quandt et al Plasmid. 2004 Jul;52(1):1-12.
    Insert
    Gentamicin resistance gene
    Use
    Helper bhr plasmid for mobilizing other plasmids …
    Promoter
    Own promoter
    Available Since
    Aug. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  30. mDsRed-Golgi-7

    Plasmid
    #55832
    Purpose
    Localization: Golgi, Excitation: 557, Emission: 585
    Depositor
    Michael Davidson
    Insert
    Golgi (B4GALT1 Human)
    Tags
    mDsRed
    Expression
    Mammalian
    Mutation
    aa 1-82 of NM_001497.3
    Promoter
    CMV
    Available Since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    55832-plasmid-map-sequence-id-494617

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  32. RGB-S reporter

    Plasmid
    #207841
    Purpose
    A three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coli
    Depositor
    Christof Niemeyer
    Article
    Zoheir et al NPJ Biofilms Microbiomes. 2023 Aug 21;9(
    Inserts
    RpoH sensing construct
    SOS sensing construct
    RpoS sensing construct
    Expression
    Bacterial
    Promoter
    PosmY, PsulA, and grpE
    Available Since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    207841-plasmid-map-sequence-id-426759

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  34. DEXsynth:ccdb-eGFP + RUBY

    Plasmid
    #233317
    Purpose
    GATEWAY compatible vector with a dexamethasone (DEX)-inducible promoter driving in frame epitope fusion of eGFP paired with ccdb selection toxin and RUBY marker
    Depositor
    Thomas Baum
    Insert
    DEXsynth:ccdb-eGFP + RUBY
    Use
    Gateway destination
    Tags
    eGFP
    Available Since
    March 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    233317-plasmid-map-sequence-id-464455

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  36. MSCV-Cre

    Plasmid
    #24064
    Purpose
    Retroviral coexpression of Cre and EGFP
    Depositor
    Dan Littman
    Insert
    Cre
    Use
    Cre/Lox and Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    MESV 5' LTR
    Available Since
    Oct. 27, 2010
    Availability
    Academic Institutions and Nonprofits only
    24064-plasmid-map-sequence-id-322907

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  38. pCS2+ H2B-TagRFP-T

    Plasmid
    #99271
    Purpose
    Contains a fusion of human histone H2B to fluorescent protein TagRFP-T in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.
    Depositor
    Philipp Keller
    Insert
    TagRFP-T
    Tags
    H2B
    Expression
    Mammalian
    Promoter
    SP6
    Available Since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    99271-plasmid-map-sequence-id-192973

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Showing: 5641 - 5680 of 173387 results