We narrowed to 24,746 results for: CHI

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pEGFP-C1-6xHis

Plasmid

#87343

Purpose

To express EGFP-6xHis in mammalian cell

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

6xHis

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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PX852

Plasmid

#62884

Purpose

C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa536-1368)

Use

CRISPR

Tags

FKBP12, NLS SV40, and NLS nucleoplasmin

Expression

Mammalian

Promoter

CBh

Available Since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-ermGFP

Plasmid

#27172

Depositor

Michela Lizier

Article

Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

Insert

erythromycin ribosomal methylase promoter region fused with modified green fluorescent protein 5

Expression

Bacterial

Mutation

N/A

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-BarcodeV5

Plasmid

#78629

Purpose

GESTALT V5 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V5

Use

Lentiviral

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-Myo1b

Plasmid

#187362

Purpose

Expresses rat myosin 1b isoform b labelled with EGFP

Depositor

Evelyne Coudrier

Article

Raposo et al Mol Biol Cell. 1999 May . 10(5):1477-94.

Insert

myosin 1b isoform b (Myo1b Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pTKDP-dhfr

Plasmid

#71323

Purpose

Donor plasmid - trimethoprim resistant donor cassette

Depositor

Thomas Kuhlman

Article

Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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PX854

Plasmid

#62886

Purpose

C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa573-1368)

Use

CRISPR

Tags

FKBP12, NLS SV40, and NLS nucleoplasmin

Expression

Mammalian

Promoter

CBh

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only

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pCAGGS-myrGFP

Plasmid

#115502

Purpose

expression of myristoylated, plasma membrane targeted GFP

Depositor

Thomas Biederer

Article

Stagi et al Proc Natl Acad Sci U S A. 2010 Apr 20;10

Insert

myristoylated GFP

Expression

Mammalian

Promoter

chicken beta-actin

Available Since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only
pmNeonGreenHO-3xFLAG-G

Plasmid

#127914

Purpose

a mammalian expression backbone vector for humanized mNeonGreen-3xFlag

Depositor

Isei Tanida

Article

Tanida-Miyake et al PLoS One. 2018 Jan 17;13(1):e0191108. do

Insert

humanized mNeonGreen

Tags

mNeonGreen-3xFlag

Expression

Mammalian

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pccGFPG83I

Plasmid

#73650

Purpose

Fluorescent reporter for transmembrane protein dimerization negative control.

Depositor

Alessandro Senes

Article

Armstrong et al Biochim Biophys Acta. 2016 Jul 21. pii:

Insert

glycophorin A- G83I

Expression

Bacterial

Mutation

glycine 83 to isoleucine

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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p104_gRNA_Sp_CD3e

Plasmid

#213780

Purpose

Expresses CRISPRi gRNA for CD3e promoter and GFP. gRNA driven by mU6.

Depositor

Howard Chang

Article

Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

Sp gRNA

Use

CRISPR and Lentiviral

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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Cyto_K0

Plasmid

#133864

Purpose

Expression of cytoplasmic Ribosome non-stalling reporter

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

Cyto_K0

Tags

EGFP and RFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET-mKelly1

Plasmid

#119782

Purpose

Expresses mKelly1 tagged N-terminally with an MHHHHHHGSG tag

Depositor

Stephen Mayo

Article

Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

Insert

mKelly1

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pET-mGinger1

Plasmid

#119784

Purpose

Expresses mGinger1 tagged N-terminally with an MHHHHHHGSG tag

Depositor

Stephen Mayo

Article

Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

Insert

mGinger1

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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ER_K0

Plasmid

#133863

Purpose

Expression of ER-targeted Ribosome non-stalling reporter

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

ER_K0

Tags

EGFP and RFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-Rab9

Plasmid

#31736

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab9 (Rab9 Fly)

Tags

GFP

Expression

Insect

Promoter

Actin

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pCWori-A13AMO-aaCPRct

Plasmid

#20117

Depositor

Jay Keasling

Article

Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

Insert

Amorphadiene oxidase and p450 reductase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only

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GESTALT_pX330-v1

Plasmid

#103061

Purpose

plasmid px330 with guide targeting GESTALT v1 through V5 constructs

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

integration of Cas9 with guide targeting GESTALT barcodes V1 to V5

Use

Lentiviral

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pJMP3666

Plasmid

#222352

Purpose

Expression vector with GFP expressed under PabstBR promoter, aprR marker, and replicates in A. baumannii and E. coli.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Insert

sfGFP

Expression

Bacterial

Promoter

PabstBR

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-mSlug

Plasmid

#34584

Depositor

Celeste Nelson

Article

Lee et al EMBO J. 2011 May 24;30(13):2662-74. doi:

Insert

Slug (Snai2 Mouse)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only
p3xFLAG-mSnail

Plasmid

#34583

Depositor

Celeste Nelson

Article

Lee et al EMBO J. 2011 May 24;30(13):2662-74. doi:

Insert

Snail (Snai1 Mouse)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2012

Availability

Academic Institutions and Nonprofits only
pMUT2-pCadBA-mCherry

Plasmid

#192865

Purpose

pMUT2 based vector for sensing pH in E. coli Nissle 1917

Depositor

Morten Sommer

Article

Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

Insert

pH sensitive promoter

Expression

Bacterial

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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Lyn-YFP-FRB1N

Plasmid

#162431

Purpose

for use in CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

Lyn-YFP-FRB1N

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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PECAM-eGFP

Plasmid

#14688

Depositor

Victoria Bautch

Article

Kearney et al Blood. 2004 Jun 15. 103(12):4527-35.

Insert

PECAM promoter/enhancer (Pecam1 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

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p105_gRNA_Sa_CD40

Plasmid

#213781

Purpose

Expresses CRISPRa gRNA for CD40 promoter and mScarlet. gRNA driven by mU6.

Depositor

Howard Chang

Article

Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

Sa gRNA

Use

CRISPR and Lentiviral

Promoter

mU6

Available Since

May 24, 2024

Availability

Academic Institutions and Nonprofits only

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p006-GFP-weak

Plasmid

#108314

Purpose

GFP from jellyfish Aquorea sp. under weak synthetic constitutive promoter pFAB4282; kanamycin resistant.

Depositor

Jaroslaw Bryk

Article

Siddall et al bioRxiv 2021.06.20.449138

Insert

Green fluorescent protein

Expression

Bacterial

Promoter

pFAB4282

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRF-BetI

Plasmid

#49357

Depositor

Christopher Voigt

Article

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

BetI repressor

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGL0_24 [pBBR1-UP ORI]

Plasmid

#198937

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBBR1-UP ORI

Use

Synthetic Biology

Mutation

S100L (C299T)

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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PX458_RAD21_1

Plasmid

#64057

Purpose

Expresses gRNA against human RAD21 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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PX458_CREB1_1

Plasmid

#64939

Purpose

Expresses gRNA against human CREB1 along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xF-NHP2

Plasmid

#126871

Purpose

Expresses 3xFlag NHP2

Depositor

Kathleen Collins

Article

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

NHP2 (NHP2 Human)

Tags

3xFlag

Expression

Mammalian

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pWS931

Plasmid

#123053

Purpose

TetR bacterial repressor

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

TetR-NLS [3a]

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pWS1806

Plasmid

#123047

Purpose

Melatonin GPCR

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

MTNR1A [3]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo-UL32

Plasmid

#162664

Purpose

Lentiviral vector for stable expression of UL32 in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

UL32

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS625

Plasmid

#123046

Purpose

Adenosine GPCR

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

A2BR [3]

Expression

Yeast

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ301

Plasmid

#102690

Purpose

S. pombe gRNA entry vector with Cas9 - Sp lys9 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-T4uvsW

Plasmid

#228201

Purpose

Expression in BL21(DE3) of T4 3' to 5' helicase/single-strand DNA annealase

Depositor

Stephen Benkovic

Article

Nelson et al J Biol Chem. 2007 Jan 5;282(1):407-16. d

Insert

uvsW

Tags

hexahistidine and 10 amino acid linker

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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5HT6-GCaMP5G

Plasmid

#48340

Purpose

Fluorescent reporter for Ca2+ inside primary cilia

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

GCaMP5G

Tags

5HT6

Expression

Mammalian

Available Since

Nov. 26, 2013

Availability

Academic Institutions and Nonprofits only
pJYP1

Plasmid

#202406

Purpose

Backbone vector to clone toxic or unstable DNA using dual-control (transcriptional control via naringenin inducible pfdeA promoter - translational control via B12 riboswitch)

Depositor

Remy Loris

Article

Vandierendonck et al Toxins (Basel). 2023 Aug 18;15(8):508. d

Type

Empty backbone

Expression

Bacterial

Promoter

fdeA

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLRD13

Plasmid

#208265

Purpose

Production of ent-cassa-12,15-diene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
ptmT2
osKSL7

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLRD05

Plasmid

#208259

Purpose

Production of isopimara-8,15-diene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
cldB
sare_1287

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLRD15

Plasmid

#208267

Purpose

Production of ent-sandaracopimaradiene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
ptmT2
osKSL10

Expression

Bacterial

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGC4593

Plasmid

#110206

Purpose

Accessory plasmid with inducible TEV protease and unnatural tRNA system (AcF)

Depositor

Adam Arkin, Guillaume Cambray

Article

Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

Inserts

TEV protease
aaRS

Expression

Bacterial

Promoter

Pbad and Ptet

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP12049

Plasmid

#222355

Purpose

Tn7 integrative vector with GFP expressed under PabstBR promoter and aprR marker on Tn7 transposon.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Insert

sfGFP

Expression

Bacterial

Promoter

PabstBR

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ294

Plasmid

#102688

Purpose

S. pombe gRNA entry vector with Cas9 - KanMX marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ300

Plasmid

#102689

Purpose

S. pombe gRNA entry vector with Cas9 - Sp his3 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-mCherry-C

Plasmid

#203326

Purpose

Express N-terminal mCherry-fused protein in mammalian cell

Depositor

Chiaki Murakami

Article

Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

Type

Empty backbone

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p(bhsp68-Cas9)

Plasmid

#65959

Purpose

Tribolium basal heat shock promoter driving Cas9

Depositor

Michalis Averof

Article

Gilles et al Development. 2015 Jul 9. pii: dev.125054

Insert

Cas9

Use

CRISPR

Tags

nuclear localization sequence

Expression

Insect

Promoter

bhsp

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-CAG-T-V5-GR-2A-H2BmCherry-IP

Plasmid

#171500

Purpose

Overexpression of T(Brachyury)-GR

Depositor

Hans Schöler

Article

Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

Insert

T

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pYZ293

Plasmid

#102687

Purpose

S. pombe gRNA entry vector with Cas9 - Sp leu1 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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