We narrowed to 6,990 results for: Cad

1. pFUW-TetO-tdTomato-cNLS

   Plasmid

   #138532

   Purpose

   Lentiviral inducible expression of tdTomato with nuclear localization signal

   Depositor

   Christopher Grigsby, Ana Teixeira

   Article

   Taylor-Weiner et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

   Insert

   tdTomato
   

   Use

   Lentiviral

   Tags

   3x SV40 cNLS

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-CMV

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Per2:iLuc

   Plasmid

   #160293

   Purpose

   target Per2 gene with a color-switching luciferase cassette in mouse genome and the transfection of the recombinant DNA will lead genomic recombination and make a knock-in allele in mouse ES cells.

   Depositor

   Joseph Takahashi

   Article

   Shan et al Neuron. 2020 Aug 4. pii: S0896-6273(20)3

   Insert

   Per2LUC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   no promotor

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 Flag mHMGB2

   Plasmid

   #31610

   Depositor

   Yasuhiko Kawakami

   Article

   Taniguchi et al Proc Natl Acad Sci U S A. 2009 Sep 29. 1

   Insert

   HMGB2 (Hmgb2 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pCEP4-myc-HLA-A1

   Plasmid

   #135503

   Purpose

   Mammalian expression of myc-tagged HLA-A*01:01

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-A*01:01 (HLA-A Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV.PGK.mLmx1a

   Plasmid

   #33013

   Depositor

   Malin Parmar

   Article

   Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

   Insert

   LIM homeobox transcription factor 1 alpha (Lmx1a Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLux01

   Plasmid

   #78281

   Purpose

   mRFP1 expressed behind an AHL-inducible construct

   Depositor

   Tom Ellis

   Article

   Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

   Insert

   AHL inducible mRFP1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. MBC2-null

   Plasmid

   #162749

   Purpose

   MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2) for expression of TCR genes

   Depositor

   Maki Nakayama

   Article

   Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

   Insert

   murine TCR-alpha signal peptide and murine TCR-beta2 constant region (TRBC2)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. MBC1-null

   Plasmid

   #162748

   Purpose

   MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genes

   Depositor

   Maki Nakayama

   Article

   Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

   Insert

   murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-CRY2-5ptaseOCRL(D523G)

   Plasmid

   #66837

   Purpose

   Expression of inositol 5-phosphatase domain of OCRL(D523G - catalytically inactive mutant). Binds to CIBN upon blue light illumination.

   Depositor

   Pietro De Camilli

   Article

   Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

   Insert

   5ptaseOCRL (OCRL Human)
   

   Use

   Tags

   mCherry-CRY2

   Expression

   Mammalian

   Mutation

   D523G

   Promoter

   CMV

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWPI-IRES-eGFP-MEFV

    Plasmid

    #92103

    Purpose

    Expression in eukaryotic cells and generating stable cell lines

    Depositor

    Feng Shao

    Article

    Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

    Insert

    MEFV (MEFV Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

11. pLenti-PGK-Hygro-amp-sgRNA-resistant PBRM1

    Plasmid

    #107407

    Purpose

    Lentiviral expression of gRNA resistant PBRM1

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    sg resistant PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    sgRNA resistant

    Promoter

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJR021

    Plasmid

    #198814

    Purpose

    Variant of yellow fluorescent protein for transient gene expression

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    15xUAS::destabilizedYFP::let-838 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    YFP with destabilized signal from mouse ornithine…

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMVhPOP1_3Flag

    Plasmid

    #53968

    Purpose

    Mammalian expression vector for human POP1 with c-terminal 3xFLAG epitopes.

    Depositor

    Martin Dorf

    Article

    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

    Insert

    POP1 (POP1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-N90-beta-catenin

    Plasmid

    #31787

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Entry vector

    Tags

    Myc

    Expression

    Mutation

    Deletion of aa 1-90- Constitutively Active

    Promoter

    Available since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA4-Beclin1-(151-241)

    Plasmid

    #24393

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Amino acids 151-241 are deleted. Delta CC mutant.

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pCMV10-Flag-Fbxl21

    Plasmid

    #48752

    Purpose

    Expression of FLAG-tagged Fbxl21

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

    Insert

    Fbxl21 (Fbxl21 Mouse)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18

    Plasmid

    #172316

    Purpose

    CRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFastBac HT JS-Rab10wt

    Plasmid

    #135557

    Purpose

    Express mouse Rab10wt in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Rab10wt.

    Depositor

    Jingshi Shen

    Article

    Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

    Insert

    Rab10 (Rab10 Mouse)
    

    Use

    Tags

    6xHis tag, TEV site

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBabe human FOXC2

    Plasmid

    #15535

    Depositor

    Bob Weinberg

    Article

    Mani et al Proc Natl Acad Sci U S A. 2007 Jun 12. 1

    Insert

    FOXC2 (FOXC2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

20. GFP-WT (AF41)

    Plasmid

    #21367

    Depositor

    Gregory Germino

    Article

    Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Use

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    wild-type sequence*

    Promoter

    Available since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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21. PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9

    Plasmid

    #186699

    Purpose

    Plasmid encoding PRDM9dC-Cas9 fusion under CMV promoter

    Depositor

    Jennifer Doudna

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

    Insert

    PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9 (PRDM9 Human)
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCI syn iGluu

    Plasmid

    #106122

    Purpose

    Fast indicator for synaptic glutamate imaging (synapsin promoter)

    Depositor

    Katalin Torok

    Article

    Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

    Insert

    iGluSnFR S72T variant
    

    Use

    Tags

    Myc and PDGFR

    Expression

    Mammalian

    Mutation

    Changed Ser 72 to Thr

    Promoter

    synapsin

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. Ad-Flag-XBP1u

    Plasmid

    #63677

    Purpose

    Adenoviral vector for XBP1u expression

    Depositor

    Qingbo Xu, Lingfang Zeng

    Article

    Zeng et al Proc Natl Acad Sci U S A. 2009 May 19;10

    Insert

    XBP1 (XBP1 Human)
    

    Use

    Adenoviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    unspliced

    Promoter

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

24. pCMV5-rat ERK2-WT

    Plasmid

    #40812

    Depositor

    Natalie Ahn

    Article

    Emrick et al Proc Natl Acad Sci U S A. 2006 Nov 28;10

    Insert

    MAPK1 (Mapk1 Rat)
    

    Use

    Tags

    His6

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pBabe HA-Runx1-neo

    Plasmid

    #45815

    Depositor

    Kevin Janes

    Article

    Wang et al Proc Natl Acad Sci U S A. 2011 Oct 4;108

    Insert

    Runx1 (Runx1 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

26. pCMV3Tag8li-hDDX50-HA

    Plasmid

    #165465

    Purpose

    Human DDX50 mammalian expression vector with C-terminal HA tag.

    Depositor

    Martin Dorf

    Article

    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

    Insert

    DExD-box helicase 50 (DDX50 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJS145

    Plasmid

    #179159

    Purpose

    Encodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.

    Depositor

    Iva Greenwald

    Article

    Shaffer et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

    Insert

    rps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Flexon replacing 2nd intron of 2xNLS::GFP

    Promoter

    rps-27p

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. E42_gRunx1_gRNA2_dTet_NGFR

    Plasmid

    #189801

    Purpose

    Retroviral delivery of guide RNA against mouse Runx1

    Depositor

    Ellen Rothenberg

    Article

    Shin et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    gRunx1_gRNA2 (Runx1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. E42_gRunx1_gRNA1_dTet_NGFR

    Plasmid

    #189802

    Purpose

    Retroviral delivery of guide RNA against mouse Runx1

    Depositor

    Ellen Rothenberg

    Article

    Shin et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    gRunx1_gRNA1 (Runx1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pT2-7xTcf-NLS-CNL

    Plasmid

    #65712

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, CNL
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge