We narrowed to 25,955 results for: Vit;

1. pCAT.015

   Plasmid

   #119558

   Purpose

   Level T acceptor vector for chromosomal integration with lacZ compatible with blue/white screening. Modified pUC19 vector.

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5 FRT/TO Plk4 KD-mCherry

   Plasmid

   #80270

   Purpose

   mammalian expression plasmid for kinase dead Plk4

   Depositor

   Andrew Holland

   Article

   Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

   Insert

   Plk4 (PLK4 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   D154A kinase dead

   Promoter

   CMV

   Available since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-BE4-RrA3F

   Plasmid

   #138340

   Purpose

   Mammalian expression plasmid for BE4-RrA3F

   Depositor

   Nicole Gaudelli

   Article

   Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

   Insert

   BE4-RrA3F
   

   Use

   Tags

   BP-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. AL13Rb-GAPC2

   Plasmid

   #161009

   Purpose

   PP7-tagged reporter driven by the Arabidopsis GAPC2 promoter

   Depositor

   Krishna Niyogi

   Article

   Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

   Insert

   PP7 and H2B-mScarlet
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-HA-mIFITM1

   Plasmid

   #58415

   Purpose

   Expresses murine IFITM1 with an N-terminal HA tag in mammalian cells.

   Depositor

   Jacob Yount

   Article

   Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

   Insert

   IFITM1 (Ifitm1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE

   Available since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pCAG-GBP1-10gly-lexADBD

   Plasmid

   #50795

   Purpose

   Expression of a GFP-dependent transcription factor component in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

   Insert

   GBP1-lexA DNA binding domain fusion protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-SV40-Galpha-i1 ONE-GO

   Plasmid

   #204328

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Insert

   SV40 Galpha-i1 ONE-GO
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-Tag2B Flag BimS

   Plasmid

   #23283

   Depositor

   Roger Davis

   Article

   Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

   Insert

   pCMV Tag2b Bim S (Bcl2l11 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pAAV-fCR-GFP

   Plasmid

   #22911

   Depositor

   Edward Callaway

   Article

   Nathanson et al Front Neural Circuits. 2009 . 3():19.

   Insert

   fugu calretinin promoter driving GFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pGL4-Gateway-SCP1

    Plasmid

    #71510

    Purpose

    humanSTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the SCP1 core promoter

    Depositor

    Alexander Stark

    Article

    Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

    Type

    Empty backbone

    Use

    Humanstarr-seq validation vector

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

11. cacnb1b (rat) in pMT2 vector

    Plasmid

    #107423

    Purpose

    Expresses Calcium channel beta1b auxillary subunit in mammalian cells.

    Depositor

    Annette Dolphin

    Article

    Page et al J Neurosci. 1997 Feb 15;17(4):1330-8.

    Insert

    calcium channel beta1b auxillary subunit
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ad MLP

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMCSG7-D16A-NmeCas9

    Plasmid

    #71476

    Purpose

    bacterial pMCSG7 expression vector expressing Nme cas9 nickase with a D16A mutation (RuvC domain) with T7 promoter, N-terminal His tag and TEV site

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

    Insert

    pMCSG7-D16A-NmeCas9
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    D16A

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMCSG7-H588A-NmeCas9

    Plasmid

    #71477

    Purpose

    bacterial pMCSG7 expression vector expressing Nme cas9 nickase with H588A mutation (HNH domain) with T7 promoter, N-terminal His tag and TEV site

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

    Insert

    pMCSG7-H588A-NmeCas9
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    H588A

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRS416gT-GalL-Cas9

    Plasmid

    #79903

    Purpose

    pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expression

    Depositor

    Ronald Davis

    Article

    Fu et al Nucleic Acids Res. 2016 May 19. pii: gkw

    Inserts

    Cas9
    guide RNA (gRNA)
    Tetracycline Repressor
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPM1 promoter, GalL, and RPR1 promoter with TetO …

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pORE303N_CEN12RRFuzzy3MYB

    Plasmid

    #194441

    Purpose

    CRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12, RR, and Fuzzy3MYB

    Depositor

    Chung-Jui Tsai

    Article

    Ortega et al New Phytol. 2022 Nov 16. doi: 10.1111/np

    Insert

    gRNA array targeting CEN1, RR, Fuzzy3MYB
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    MtU6

    Available since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pT7-th-sgRNA

    Plasmid

    #65565

    Purpose

    in vitro trancription of sgRNA targeting the zebrafish th locus

    Depositor

    Jiu-lin Du

    Article

    Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

    Insert

    th sgRNA (th Zebrafish)
    

    Use

    In vitro rna transcription

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRN3P_T3_BE3_IVT

    Plasmid

    #177015

    Purpose

    Plasmid to be used as DNA template for in-vitro RNA transcription of the BE3 base editor (C to T) by T3 RNA polymerase

    Depositor

    Timo Otonkoski

    Article

    Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

    Insert

    BE3
    

    Use

    CRISPR; Vector for in-vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T3 promoter

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP.N3-SLC35A2-GFP

    Plasmid

    #186284

    Purpose

    transient expression of C-terminal GFP tagged SLC35A2 isoform c/UGT1 in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Li et al Glycobiology. 2019 Jun 1;29(6):490-503.

    Insert

    SLC35A2 (SLC35A2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NpT7-5-ERK1

    Plasmid

    #39229

    Depositor

    Melanie Cobb

    Article

    Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

    Insert

    ERK1 (MAPK3 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pGEX-2T FRB S2035I

    Plasmid

    #26608

    Depositor

    Jie Chen

    Article

    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    FRB domain only (aa 2015-2114); S2035I

    Promoter

    Available since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

21. pM115: CasRx control presgRNA

    Plasmid

    #166867

    Purpose

    U6-driven expression of control (non-targeting) presgRNA compatible with CasRx.

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Insert

    non-targeting presgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. SENP2M497A

    Plasmid

    #61088

    Purpose

    cleave the SUMO off when use PETSUMO vector for express fusion protein

    Depositor

    Cynthia Wolberger

    Article

    Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

    Insert

    SENP2M497A catalytic domain (SENP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M497A; Catalytic domain is residues 364-589

    Promoter

    T7

    Available since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

23. p3E-mCherry-NS-MCS

    Plasmid

    #75179

    Purpose

    mCherry (red) fluorophore with multiple cloning site for use with inducible N-terminal fusions.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    mCherry-NS-MCS
    

    Use

    Three prime entry vector for multisite gateway th…

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CBP-HAT-pET

    Plasmid

    #21091

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

    Insert

    CBP (Crebbp Mouse)
    

    Use

    Tags

    His and T7 tag

    Expression

    Bacterial

    Mutation

    HAT domain aa 1171-1621

    Promoter

    Available since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

25. pJM126

    Plasmid

    #49339

    Purpose

    bacterial expression of RFA1, RFA2, and RFA3

    Depositor

    Steven Brill

    Article

    He et al J Biol Chem. 1996 Nov 8;271(45):28243-9.

    Inserts

    RFA3 (RFA3 Budding Yeast)
    RFA2 (RFA2 Budding Yeast)
    RFA1 (RFA1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pCX-2A-2A

    Plasmid

    #74670

    Purpose

    based on pCX-C1 with F2A1/F2A2 inserted

    Depositor

    Roberto Giovannoni

    Article

    De Giorgi et al Plasmid. 2015 May;79:22-9. doi: 10.1016/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA5 FRT/TO MycLAP-STIL 5A

    Plasmid

    #80267

    Purpose

    mammalian expression plasmid for MycLAP-STIL 5A

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    STIL (STIL Human)
    

    Use

    Tags

    Myc-GFP

    Expression

    Mammalian

    Mutation

    S1103A, S1108A, S1111A, S1116A, T1119A

    Promoter

    CMV

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGC629

    Plasmid

    #71364

    Purpose

    proximal somatic gonad expression of DAF-16 in C.elegans

    Depositor

    Jane Hubbard

    Article

    Qin et al Nat Commun. 2015 May 11;6:7107. doi: 10.

    Insert

    daf-16 (daf-16 Nematode)
    

    Use

    Tags

    GFP(65C)

    Expression

    Worm

    Mutation

    Promoter

    fos1a promoter

    Available since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pegRNA_HEK4_G-T

    Plasmid

    #201975

    Purpose

    Express a pegRNA used for G to T conversion at HEK4 site

    Depositor

    Scot Wolfe

    Article

    Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

    Insert

    pegRNA_HEK4_G-T
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pegRNA_HEK4_1bp-deletion

    Plasmid

    #201976

    Purpose

    Express a pegRNA used for 1bp deletion at HEK4 site

    Depositor

    Scot Wolfe

    Article

    Liang et al Nat Methods. 2023 Jun;20(6):898-907. doi

    Insert

    pegRNA_HEK4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. SlugBE4max

    Plasmid

    #163799

    Purpose

    Expresses AncBE4max and nSlugCas9

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    SlugCas9 (D10A)

    Promoter

    Available since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Flag TSC2 2A(S939A + S981A)

    Plasmid

    #12131

    Depositor

    Cheryl Walker

    Article

    Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

    Insert

    TSC2 S939A S981A (TSC2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S939A. S981A.

    Promoter

    Available since

    July 6, 2006

    Availability

    Academic Institutions and Nonprofits only

33. UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2

    Plasmid

    #87644

    Purpose

    For creating drosophila transgenics expressing UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2

    Depositor

    Gregory Jefferis

    Article

    Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

    Insert

    myr::SNAPf>myr::CLIPf>myr::Halo2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pET28a-pro-sumo3

    Plasmid

    #25103

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    pro-SUMO3 (SUMO3 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    changed Serine 2 to Alanine

    Promoter

    Available since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

35. pCMV-Tag2B BimEL (3SA)

    Plasmid

    #23091

    Depositor

    Roger Davis

    Article

    Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

    Insert

    Bim EL (3SA) (Bcl2l11 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed Serine 55/65/73 to alanine

    Promoter

    Available since

    Feb. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

36. pLentiGuide_GFP_sgEPAS1#1

    Plasmid

    #183119

    Purpose

    pLentiGuide modified to express GFP and an sgRNa targeting EPAS1

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    sgEPAS1-1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLentiGuide_GFP_sgEPAS1#2

    Plasmid

    #183120

    Purpose

    pLentiGuide modified to express GFP and an sgRNA targeting EPAS1

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    EPAS1-2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCAFNF-tdTomato

    Plasmid

    #125575

    Purpose

    Flp-dependent expression of tdTomato in mammalian cells.

    Depositor

    Takeshi Imai

    Article

    Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

    Insert

    tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. RCAS-myc

    Plasmid

    #11548

    Depositor

    Sandra Orsulic

    Article

    Orsulic et al Cancer Cell. 2002 Feb . 1(1):53-62.

    Insert

    c-myc (MYC Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

40. pCAG-Gdf9

    Plasmid

    #163599

    Purpose

    Expresses GDF-9 in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Gdf9 (Gdf9 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge