167,601 results

1. pCM184

   Plasmid

   #46012

   Depositor

   Mary Lidstrom

   Article

   Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

   Type

   Empty backbone

   Use

   Cre/Lox

   Promoter

   na

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS

   Plasmid

   #71396

   Purpose

   2Gi2R assay

   Depositor

   Fred Gage

   Article

   Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

   Inserts

   2xGFP:NES
   2xRFP:NLS
   

   Use

   Lentiviral

   Tags

   IRES

   Expression

   Mammalian

   Promoter

   EF1alpha and IRES

   Available Since

   Dec. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pWH1266-Apra-sgRNA

   Plasmid

   #218372

   Purpose

   sgRNA expression plasmid for CRISPR-intereference

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Insert

   sgRNA expression cassette
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. eMagBF-TgRFPT

   Plasmid

   #162253

   Purpose

   Blue-light dependent heterodimerization system

   Depositor

   Pietro De Camilli

   Article

   Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

   Insert

   eMagBF-TgRFPT
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C1 NFAT3

   Plasmid

   #10961

   Depositor

   Toren Finkel

   Article

   Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

   Insert

   NFAT3 (NFATC4 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Flag-IDH1

   Plasmid

   #62906

   Purpose

   Expresses wildtype IDH1 in mammalian cells

   Depositor

   Yue Xiong

   Article

   Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

   Insert

   isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti CMV TetR Blast (716-1)

   Plasmid

   #17492

   Purpose

   3rd gen lentiviral Tetracycline repressor expression vector, CMV promoter, Blast 

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Insert

   Tetracycline repressor
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMpGE011

   Plasmid

   #71537

   Purpose

   For CRISPR/Cas9 genome editing in Marchantia polymorpha

   Depositor

   Takayuki Kohchi

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-GFP-NMHC II-A

   Plasmid

   #11347

   Depositor

   Robert Adelstein

   Article

   Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

   Insert

   NMHC II-A (MYH9 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. shCD44-2 pRRL

    Plasmid

    #19123

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Insert

    small hairpin RNA against CD44 (CD44 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.Syn.GCaMP6m.WPRE.SV40 (AAV5)

    Viral Prep

    #100841-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV.Syn.GCaMP6m.WPRE.SV40 (#100841). In addition to the viral particles, you will also receive purified pAAV.Syn.GCaMP6m.WPRE.SV40 plasmid DNA. Syn-driven GCaMP6m calcium sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Promoter

    Syn

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

12. VPS13A^mCherry

    Plasmid

    #118758

    Purpose

    VPS13A internally tagged with mCherry at residue 1372.

    Depositor

    Pietro De Camilli

    Article

    Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

    Insert

    VPS13A (VPS13A Human)
    

    Tags

    mCherry inserted at position 1372

    Expression

    Mammalian

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT2/HB

    Plasmid

    #26557

    Depositor

    Perry Hackett

    Insert

    pT2/HB
    

    Expression

    Bacterial

    Available Since

    Dec. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5FRT/Flag-KIAA1267

    Plasmid

    #29758

    Depositor

    Joan Conaway

    Article

    Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

    Insert

    KIAA1267 (KANSL1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Jan. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-PGK-Venus-Fluc (puro)

    Plasmid

    #140328

    Purpose

    Lentivirus for expression of Venus-Fluc (firefly luciferase) fusion protein

    Depositor

    Roland Friedel

    Article

    Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

    Insert

    Venus-Firefly Luciferase (luc2)
    

    Use

    Lentiviral

    Promoter

    human PGK promoter

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGP-CMV-GCaMP6m

    Plasmid

    #40754

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    GENIE Project, Douglas Kim

    Article

    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

    Insert

    GCaMP6m
    

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-mDlx-jGCaMP8m-WPRE (AAV9)

    Viral Prep

    #176754-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from AAV-mDlx-jGCaMP8m-WPRE (#176754). In addition to the viral particles, you will also receive purified AAV-mDlx-jGCaMP8m-WPRE plasmid DNA. mDlx enhancer-driven expression of calcium sensor GCaMP8m in GABA-ergic interneurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Promoter

    mDlx

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

18. pAAV-CaMKIIa-hChR2(H134R)-mCherry (AAV5)

    Viral Prep

    #26975-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-CaMKIIa-hChR2(H134R)-mCherry (#26975). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-hChR2(H134R)-mCherry plasmid DNA. CaMKIIa-driven, humanized channelrhodopsin H134R mutant fused to mCherry for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    CaMKII

    Tags

    mCherry

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

19. pLVpuro-CMV-N-mCherry

    Plasmid

    #123221

    Purpose

    N-terminal mCherry tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBBR1k-con-GFPuv

    Plasmid

    #106386

    Purpose

    Constitutive trc promoter expressing gfpuv on BBR1 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    GFPuv
    

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pET_RTX

    Plasmid

    #102787

    Purpose

    Expresses the Reverse Transcriptase gene

    Depositor

    Andrew Ellington

    Article

    Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

    Insert

    reverse transcription xenopolymerase
    

    Expression

    Bacterial

    Promoter

    T7 promoter + lac operator

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCI-EGFP-NR2a wt

    Plasmid

    #45445

    Depositor

    Andres Barria, Robert Malinow

    Article

    Barria et al Neuron. 2002 Jul 18;35(2):345-53.

    Insert

    NR2A (Grin2a Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EGFP inserted after the predicted signal peptide …

    Promoter

    CMV

    Available Since

    June 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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23. pDEST17-Cdc34a

    Plasmid

    #18674

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Cdc34A (CDC34 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

24. pQE-60 roGFP2-Orp1-His

    Plasmid

    #64976

    Purpose

    Bacterial expression of His-tagged roGFP2-Orp1

    Depositor

    Tobias Dick

    Article

    Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Tags

    His and roGFP2

    Expression

    Bacterial

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

    Plasmid

    #105842

    Purpose

    Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment

    Depositor

    Michael Ward

    Article

    Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

    Inserts

    Neurogenin 2 (NEUROG2 Human)
    ISL LIM Homeobox 1 (ISL1 Human)
    LIM Homeobox 3 (LHX3 Human)
    Optimized mApple
    rtTA3G
    SBP Delta-LNGFR
    

    Use

    CRISPR and TALEN

    Promoter

    CAG, EF-1alpha, and TRE3G

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. WT FL USP7

    Plasmid

    #227214

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Promoter

    T7-promoter

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AAVS1-Bxb1-LP-v2-TC

    Plasmid

    #194326

    Purpose

    AAVS1 targeting construct with the Bxb1 landing pad version 2 cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Stem Cell Res. 2023 Feb;66:102991. doi:

    Insert

    loxP-EBFP2-attP-BleoR-lox251
    

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pcDNA3.1_AGIA-AirID

    Plasmid

    #182210

    Purpose

    Express AirID in mammalian cells.

    Depositor

    Tatsuya Sawasaki, Sohei Ito, Akira Nozawa, Shogo Nakano

    Article

    Kido et al Elife. 2020 May 11;9. pii: 54983. doi: 1

    Insert

    AGIA-AirID
    

    Tags

    AGIA-tag

    Expression

    Mammalian

    Available Since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. pmCherry Paxillin

    Plasmid

    #50526

    Depositor

    Kenneth Yamada

    Insert

    PXN paxillin (PXN Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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30. SpCas9 TadCBEd

    Plasmid

    #193835

    Purpose

    Express TadCBEd (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDd-SpCas9 D10A-2xUGI
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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31. pAdTrack

    Plasmid

    #16404

    Purpose

    Shuttle vector for us in AdEasy System. Used for expression of transgenes under a chosen (non-CMV) promoter when a GFP tracer is desired.

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Type

    Empty backbone

    Use

    Adenoviral

    Expression

    Mammalian

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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32. pJMP1356

    Plasmid

    #119276

    Purpose

    for cloning new sgRNAs

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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33. pBK2044-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2

    Plasmid

    #223164

    Purpose

    2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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34. pATetO 6XHis T5 Exonuclease

    Plasmid

    #206209

    Purpose

    Inducible expression of T5 exonuclease

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2018 Aug 15;13(8):e0201681. do

    Insert

    T5 exonuclease
    

    Expression

    Bacterial

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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35. pLEX307-APN-blast

    Plasmid

    #158455

    Purpose

    Constitutive lentiviral expression of human APN.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    APN (ANPEP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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36. pSNAPf-N1

    Plasmid

    #58187

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    SNAP tag

    Expression

    Mammalian

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pBeloBac11loxP2272

    Plasmid

    #60342

    Purpose

    This is a BAC vector that contains both wt and mutant loxP sites.

    Depositor

    Jon Coren

    Article

    Coren et al BMC Res Notes. 2017 Jul 28;10(1):344. do

    Type

    Empty backbone

    Use

    Cre/Lox

    Expression

    Bacterial

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PJEBAN6

    Plasmid

    #203684

    Purpose

    Expresses red fluorescent marker DsRed-Express in Listeria monocytogenes

    Depositor

    Tine Rask Licht

    Article

    Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

    Insert

    DsRed-Express
    

    Expression

    Bacterial

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET His6 MBP TEV LIC cloning vector (1M)

    Plasmid

    #29656

    Depositor

    Scott Gradia

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    6xHis, MBP, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Raptor_2 shRNA

    Plasmid

    #1858

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    Raptor (RPTOR Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge