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AB.pCCL.sin.cPPT.GFP.miR-126-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85908

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-126-3p Sensor (MIR126 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTK-Slug

Plasmid

#36986

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

slug (Snai2 Mouse)

Use

Lentiviral

Available Since

May 31, 2012

Availability

Academic Institutions and Nonprofits only

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pRSET FLII12Pglu-700uDelta6

Plasmid

#13568

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

FLIPglu F16A Y12S-ECFP-D13R, 6aa linker

Tags

Citrine

Expression

Bacterial

Mutation

F16A, ECFP insert in mglB

Available Since

Feb. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pRN3P_AID-Dam-only

Plasmid

#136065

Purpose

in vitro transcription for mRNA

Depositor

Maria-Elena Torres-Padilla

Article

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

AID-Dam only

Use

In vitro transcription

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pmVenus-C2-MmMKL2

Plasmid

#67894

Purpose

Expresses a fusion of mVenus with MKL2 (MRTF-B) in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mVenus-MKL2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Mef2A-shRNA

Plasmid

#32899

Depositor

Michael Greenberg

Article

Flavell et al Science. 2006 Feb 17;311(5763):1008-12.

Insert

Mef2a (Mef2a Rat)

Use

RNAi

Expression

Mammalian

Promoter

H1-RNA

Available Since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-GST-AGO1

Plasmid

#24316

Depositor

Carl Novina

Article

Wang et al Nat Struct Mol Biol. 2009 Dec . 16(12):1

Insert

human AGO1 (AGO1 Human)

Tags

GST

Expression

Bacterial

Available Since

April 7, 2010

Availability

Academic Institutions and Nonprofits only
pDSAR-Cre-F2A-I-SceI

Plasmid

#62302

Purpose

Anopheles docking site transgenesis plasmid expressing Cre recombinase and I-SceI nuclease, vasa promoter, DsRed marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Insert

Cre recombinase, I-SceI nuclease

Expression

Insect

Promoter

vasa

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 sgIREB2_1

Plasmid

#169894

Purpose

Disrupt IREB2

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

sgRNA targeting IREB2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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H6hSDHA

Plasmid

#113045

Purpose

Hetero expression of His-tagged human SDHA

Depositor

Edward A. Berry

Article

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

H6hSDHA (SDHA Human)

Tags

His6 (MGSSHHHHHHSQDP-)

Expression

Bacterial

Mutation

Insert begins at amino acid 43

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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HA-DN-14-3-3 zeta (R56A/R60A)

Plasmid

#116889

Purpose

Expresses HA-tagged human dominant-negative 14-3-3 zeta (R56A/R60A) in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Article

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

DN-14-3-3 zeta (R56A/R60A) (YWHAZ Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pYX233

Plasmid

#163762

Purpose

Empty vector for Gal-inducible yeast expression

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Type

Empty backbone

Tags

Hemagglutinin (HA) tag

Expression

Yeast

Promoter

GAL1

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Bik

Plasmid

#10952

Depositor

Bingliang Fang

Article

Kagawa et al Cancer Res. 2001 Apr 15. 61(8):3330-8.

Insert

human Bik gene (BIK Human)

Tags

GFP

Expression

Mammalian

Mutation

NA

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3

Plasmid

#112007

Purpose

For co-expression of GCaMP6s with mRuby3

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV9

Insert

GCaMP6s-P2A-mRuby3

Use

AAV

Expression

Mammalian

Promoter

hSynapsin1

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-Sgo1 N61I

Plasmid

#108496

Purpose

expression of vsv-Sgo1 N61I (no PP2A binding)

Depositor

Susanne Lens

Article

Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

Insert

Shugoshin 1 (SGO1 Human)

Tags

VSV

Expression

Mammalian

Mutation

no PP2A binding N61I

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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ECFP(W66A)-FRB-MoA

Plasmid

#67904

Purpose

Expresses a Mitochondria located FRB domain in mammalian cells (the ECFP is not fluorescent)

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

ECFP(W66A)-FRB-MoA

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCW-LIC-N8C_Fab4a

Plasmid

#203690

Purpose

Expresses N8C_Fab4a

Depositor

Brenda Schulman

Article

Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

Insert

N8C_Fab4a

Expression

Bacterial

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-iCre

Plasmid

#125577

Purpose

Expresses iCre under the CAG promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

iCre

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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MARCKS(1-40)-paGFP

Plasmid

#18695

Depositor

Karel Svoboda

Article

Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

Insert

MARCKS(1-40) (MARCKS Human)

Tags

photoactivatable GFP

Expression

Mammalian

Mutation

A206K mutation in paGFP

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pCAG-p65AD-GBP6

Plasmid

#49440

Purpose

Expression of a GFP-dependent transcription factor component in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

GBP6-p65 transactivation domain fusion protein

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-14-GINIP-Nluc

Plasmid

#223556

Purpose

Plasmid expressing GINIP-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Luebbers et al Sci Signal. 2025 Mar 25;18(879):eadp6457

Insert

human GINIP - linker(GGGS) - Nluc

Tags

3xFLAG

Expression

Mammalian

Available Since

March 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-Tag2B Flag BimEL

Plasmid

#23090

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (Bcl2l11 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
pLJM60-S6K1(wt) barcoded

Plasmid

#48801

Purpose

expresses wt S6K1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

S6K1 (Rps6kb1 Rat)

Use

Lentiviral

Tags

FLAG and barcode: GGATCC

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only
mNeonGreen-IETD-NanoLuc

Plasmid

#98288

Purpose

caspase-8 BRET Sensor

Depositor

Maarten Merkx

Article

den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

Insert

mNeonGreen-LEHD-NanoLuc

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Gdf2

Plasmid

#163595

Purpose

Expresses GDF-2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf2 (Gdf2 Mouse)

Expression

Mammalian

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-KB2123-Nluc

Plasmid

#204325

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

KB2123-Nluc

Expression

Mammalian

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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p3E-mTagBFP

Plasmid

#75175

Purpose

TagBFP (blue) fluorophore with stop codon for C-terminal fusions

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

TagBFP

Use

Three prime entry vector for multisite gateway th…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiGuide_mCherry_sgEPAS1#1

Plasmid

#183122

Purpose

pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

sgEPAS1-1

Use

Lentiviral

Promoter

U6

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pPyCAG-tagBFP-3xNLS-IRES-Hph

Plasmid

#183608

Purpose

Mammalian expression vector for tagBFP-3xNLS from the CAG promoter. Confers hygromycin B resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

tagBFP-3xNLS

Tags

3xNLS

Expression

Mammalian

Promoter

CAG

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ABKAR(T/A)

Plasmid

#140052

Purpose

Negative control mutant for ABKAR biosensor.

Depositor

Jin Zhang

Article

Sample et al Mol Biol Cell. 2015 May 15;26(10):1935-4

Insert

ABKAR(T/A)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Available Since

Nov. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-Gateway-DSCP

Plasmid

#71506

Purpose

flySTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the DSCP core promoter

Depositor

Alexander Stark

Article

Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

Type

Empty backbone

Use

Flystarr-seq validation vector

Tags

luciferase

Expression

Insect

Available Since

Dec. 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgRNA(MS2)-puro-barcode

Plasmid

#89493

Purpose

Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

Depositor

Gary Hon

Article

Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

Insert

sgRNA (with MS2 loop)

Use

Lentiviral

Promoter

U6

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM60 mUlk1 (wt) barcoded

Plasmid

#48799

Purpose

expresses wt Ulk1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

ULK1 (Ulk1 Mouse)

Use

Lentiviral

Tags

FLAG and barcode: GGATCC

Expression

Mammalian

Promoter

CMV

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only
pBT44c

Plasmid

#138511

Purpose

P2

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

inteinC-dSpCas9-UGI

Use

Synthetic Biology

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
FRB-ECFP(W66A)-Giantin

Plasmid

#67903

Purpose

Expresses a Golgi located FRB domain in mammalian cells (the ECFP is not fluorescent)

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

FRB-ECFP(W66A)-Giantin

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-mCherry-2xEcMTS

Plasmid

#133624

Purpose

Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

Depositor

Petra Schwille

Article

Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

Insert

mCherry-2xEcMTS

Tags

His

Expression

Bacterial

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRN3P_T3_ABEmax_IVT

Plasmid

#171761

Purpose

Plasmid to be used as DNA template for in-vitro RNA transcription of the ABEmax base editor (A to G) by T3 RNA polymerase

Depositor

Timo Otonkoski

Article

Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

Insert

ABEmax

Use

Vector for in-vitro transcription

Promoter

T3 promoter

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pEJS2156_Nme2-ABE8e-nt

Plasmid

#201503

Purpose

Mammalian expression of N-terminally fused Nme2-ABE8e-nt

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-nt

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p-mCherry-N1-ChloC

Plasmid

#85461

Purpose

Historical plasmid. For inhibition of neuronal activity, #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)

Depositor

Peter Hegemann

Article

Wietek et al Science. 2014 Mar 27.

Insert

ChloC

Tags

mCherry

Expression

Mammalian

Mutation

E90R, T159C

Promoter

CMV (+enhancer)

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-E1.1-RFP

Plasmid

#22928

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

E1.1 binding site from Scardigli et al., 2003 with minimal CMV

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge

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