Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

169,450 results

Showing: 7501 - 7550 of 169450 results

  4. Mm-PylRS-AF/Pyl-tRNACUA

    Plasmid
    #122650
    Purpose
    tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    PylRS-AF, Pyl-tRNACUA
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Tyrosine 306 changed to Alanine, Tyrosine 384 cha…
    Available Since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    122650-plasmid-map-sequence-id-437776

    Enlarge

  9. pCEP4-PDGFRA-reporter

    Plasmid
    #136456
    Purpose
    Mammalian fluorescent reporter plasmid for PDGFRA signaling.
    Depositor
    Erik Procko
    Article
    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.
    Inserts
    PDGFRalpha (PDGFRA Human)
    Array of serum response elements (SRE) to drive destabilized GFP expression from a minimal promoter.
    Tags
    Extracellular c-myc epitope tag, Signal peptide f…
    Expression
    Mammalian
    Promoter
    CMV and Minimal promoter with artificial SRE enha…
    Available Since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    136456-plasmid-map-sequence-id-263271

    Enlarge

  10. pHM106Env

    Plasmid
    #139024
    Purpose
    Express HIV Env which is isolated a from IRB approved HIV infected patient samples from Singapore and cloned into expression vector (pcDNA 3.1)
    Depositor
    Anjali Joshi
    Article
    Joshi et al Virology. 2017 Dec;512:222-233. doi: 10.
    Insert
    HIV-1 Envelope Glycoprotein (env HIV-1)
    Expression
    Mammalian
    Available Since
    April 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    139024-plasmid-map-sequence-id-288127

    Enlarge

  11. MoClo Plant Parts II and Infrastructure Kit

    Plasmid Kit
    #1000000135
    Purpose
    Compatible with the Modular Cloning (MoClo) system for hierarchical assembly of plant multigene constructs, with new phytobricks and vectors to connect Modular Cloning and Gateway Cloning platforms.
    Depositor
    Johannes Stuttmann
    Available Since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  14. pDest-Tol2 (Chien)-Tol2-Isl2b:GFP:polyA

    Plasmid
    #105648
    Purpose
    Expression of GFP in sensory neurons (retinal ganglion cells, trigeminal, Rohon Beard neurons and dorsal root ganglia) for up to 2 weeks
    Depositor
    Sandra Rieger
    Insert
    Tol2-Isl2b:GFP:polyA (isl2b Zebrafish)
    Expression
    Mammalian
    Available Since
    Feb. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    105648-plasmid-map-sequence-id-202869

    Enlarge

  16. pM-Cas9

    Plasmid
    #196325
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding SpCas9 in place of viral GP
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding SpCas9 in place of viral GP
    Use
    CRISPR
    Tags
    3×flag
    Expression
    Plant
    Mutation
    See depositor comments below
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available Since
    May 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    196325-plasmid-map-sequence-id-390015

    Enlarge

  23. pET23b-15F11-HA-mEGFP-6xHis

    Plasmid
    #129593
    Purpose
    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP and His-tag. This plasmid is for bacteria protein expression under T7 promoter and protein purification with HisTrap.
    Depositor
    Tim Stasevich
    Article
    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:
    Insert
    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    Tags
    6xHis-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 15, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    129593-plasmid-map-sequence-id-254271

    Enlarge

  25. pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2]

    Plasmid
    #84445
    Purpose
    AAV-mediated expression of Jaws-KGC-GFP-ER2 under the CAG promoter, in floxed/reversed (Cre-dependent) manner.
    Depositor
    Edward Boyden
    Article
    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi
    Has Service
    AAV Retrograde, AAV1, and AAV5
    Insert
    Jaws-KGC-GFP-ER2
    Use
    AAV
    Tags
    ER2, GFP, and KGC
    Expression
    Mammalian
    Mutation
    K200R W214F
    Promoter
    CAG
    Available Since
    April 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    84445-plasmid-map-sequence-id-177041

    Enlarge

  26. OmpF

    Plasmid
    #213135
    Purpose
    Expresses outer membrane protein F
    Depositor
    Sebastian Hiller
    Insert
    OmpF (23-362) (ompF E.coli (K12))
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    213135-plasmid-map-sequence-id-422154

    Enlarge

  28. pENN.AAV.EF1a.eGFP.WPRE.rBG (AAV8)

    Viral Prep
    #105547-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pENN.AAV.EF1a.eGFP.WPRE.rBG (#105547). In addition to the viral particles, you will also receive purified pENN.AAV.EF1a.eGFP.WPRE.rBG plasmid DNA. EF1a-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    James M. Wilson
    Promoter
    EF1a
    Tags
    EGFP
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only

  31. pSico

    Plasmid
    #11578
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes EGFP to be recombined out of the construct, activating shRNA expression.
    Depositor
    Tyler Jacks
    Article
    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Available Since
    July 19, 2006
    Availability
    Academic Institutions and Nonprofits only
    11578-plasmid-map-sequence-id-106042

    Enlarge

  32. pAAV-CAG-NIR-GECO2G (AAV1)

    Viral Prep
    #159605-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CAG-NIR-GECO2G (#159605). In addition to the viral particles, you will also receive purified pAAV-CAG-NIR-GECO2G plasmid DNA. CAG-driven expression of the near-infrared calcium indicator NIR-GECO2G. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Robert Campbell
    Article
    Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.
    Promoter
    CAG
    Available Since
    Dec. 18, 2024
    Availability
    Academic Institutions and Nonprofits only

  35. pAAV.CMV.LacZ.bGH

    Plasmid
    #105531
    Purpose
    AAV expression of LacZ from CMV promoter
    Depositor
    James M. Wilson
    Has Service
    AAV5 and AAV8
    Insert
    LacZ
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105531-plasmid-map-sequence-id-202365

    Enlarge

  39. pDRM210 LGP (Luciferase GFP Puro)

    Plasmid
    #174722
    Purpose
    Expresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance gene
    Depositor
    Wayne Tilley
    Article
    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413
    Inserts
    Firefly Luciferase
    eGFP
    pac
    Use
    Lentiviral and Luciferase
    Tags
    E2A linker and F2A linker
    Expression
    Mammalian
    Promoter
    MND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…
    Available Since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    174722-plasmid-map-sequence-id-348963

    Enlarge

  44. TFORF3317

    Plasmid
    #144793
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF75A (ZNF75A Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    144793-plasmid-map-sequence-id-353422

    Enlarge

  50. pSLPB2B-FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187951
    Purpose
    FKBP12 (F36V mutant) degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    FKBP12_F36V-SpdCas9-tagRFPt-P2A-tagBFP
    Use
    CRISPR and Synthetic Biology
    Tags
    GGS linker
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187951-plasmid-map-sequence-id-373504

    Enlarge

Showing: 7501 - 7550 of 169450 results