We narrowed to 18,818 results for: RAN-1

1. nSaCas9 D126 miniABEMax V82G

   Plasmid

   #135367

   Purpose

   Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (D126 )

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSaCas9 D126 miniABEMax V82G
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. nSaCas9 E739 miniABEMax V82G

   Plasmid

   #135368

   Purpose

   Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E739 )

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSaCas9 E739 miniABEMax V82G
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. nSaCas9 E743 miniABEMax V82G

   Plasmid

   #135369

   Purpose

   Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E743 )

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSaCas9 E743 miniABEMax V82G
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. nSaCas9 E745 miniABEMax V82G

   Plasmid

   #135370

   Purpose

   Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E745 )

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSaCas9 E745 miniABEMax V82G
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. nSaCas9 F733 miniABEMax V82G

   Plasmid

   #135371

   Purpose

   Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (F733 )

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSaCas9 F733 miniABEMax V82G
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. nSaCas9 N730 miniABEMax V82G

   Plasmid

   #135372

   Purpose

   Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N730 )

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSaCas9 N730 miniABEMax V82G
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. nSaCas9 E125 miniABEMax V82G

   Plasmid

   #135373

   Purpose

   Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E125 )

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSaCas9 E125 miniABEMax V82G
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. nSaCas9 E131 miniABEMax V82G

   Plasmid

   #135374

   Purpose

   Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E131 )

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSaCas9 E131 miniABEMax V82G
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. nSaCas9 L132 miniABEMax V82G

   Plasmid

   #135375

   Purpose

   Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (L132 )

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSaCas9 L132 miniABEMax V82G
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   (V82G)

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. nSaCas9 N130 miniABEMax V82G

    Plasmid

    #135376

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N130 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9-ABEMax(V82G) N130
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. nSaCas9 E735 miniABEMax V82G

    Plasmid

    #135377

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (E735 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 E735 miniABEMax V82G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. nSaCas9 G129 miniABEMax V82G

    Plasmid

    #135380

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (G129 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 G129 miniABEMax V82G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. nSaCas9 H119 miniABEMax V82G

    Plasmid

    #135381

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (H119 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9-ABEMax(V82G) H119
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. nSaCas9 M732 miniABEMax V82G

    Plasmid

    #135382

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M732 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 M732 miniABEMax V82G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. nSaCas9 M741 miniABEMax V82G

    Plasmid

    #135383

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (M741 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 M741 miniABEMax V82G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. nSaCas9 N120 miniABEMax V82G

    Plasmid

    #135384

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (N120 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 N120 miniABEMax V82G
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. S2 1058 ABEMax

    Plasmid

    #135356

    Purpose

    Expression of intradomain insertion of ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at position 1058 of nSpyCas9

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSpyCas9-ABEMax aa 1058
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. S2 1058 V82G ABEMax

    Plasmid

    #135361

    Purpose

    Expression of intradomain insertion of miniABEMax (evolved Tada monomer with V82G) at position 1058 of nSpyCas9

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    1058 nSpyCas9 V82G ABEMax
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. miniABEMax V82G nSaCas9

    Plasmid

    #135365

    Purpose

    Expression of SaCas9 nickase (D10A) with single evolved TadA monomer (ABEMax) with a V82G substitution

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    miniABEMax V82G nSaCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    (V82G)

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AIDx-ID-202

    Plasmid

    #135344

    Purpose

    Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 202 of nSpyCas9 used for base editing

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSpyCas9-hAIDx-ID-202
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pINS-Puro

    Plasmid

    #31389

    Depositor

    Yegor Vassetzky

    Article

    Dmitriev et al BMC Res Notes. 2008 Dec 23;1:135. doi: 1

    Insert

    pac
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. DMAP

    Plasmid

    #25162

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DMAP1_14 (DMAP1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

23. pCH1/RAD51o

    Plasmid

    #102562

    Purpose

    Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon

    Depositor

    Maria Spies

    Article

    Subramanyam et al Proc Natl Acad Sci U S A. 2016 Oct 11;11

    Inserts

    Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Synthetic, Human)
    E. coli groE operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli Expression

    Promoter

    T7

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. FLAG-LoxP-PURO-LoxP-SNAP-TERT

    Plasmid

    #71390

    Purpose

    Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection.

    Depositor

    Thomas Cech

    Article

    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:

    Insert

    TERT (TERT Human)
    

    Use

    Hr donor

    Tags

    FLAG-SNAP

    Expression

    Mutation

    Promoter

    Endogenous TERT promotor

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAV-hSyn-Ribo-jGCaMP8s

    Plasmid

    #167572

    Purpose

    DEPRECATED: Please see Addgene item #169247 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Has Service

    AAV1

    Insert

    Ribo-GCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AAV-hSyn-Ribo-jGCaMP8m

    Plasmid

    #167574

    Purpose

    DEPRECATED: Please see Addgene item #169248 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Has Service

    AAV1

    Insert

    Ribo-GCaMP8m
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV_iRFP_P2AT2A_mScarlet-I_Myl9

    Plasmid

    #172474

    Purpose

    Mammalian expression of myosin light chain (Myl9) tagged with mScarlet-I and cytoplasmic iRFP as a reference marker.

    Depositor

    Sean R Collins

    Article

    Hadjitheodorou et al Nat Commun. 2021 Nov 16;12(1):6619. doi:

    Inserts

    iRFP670
    mScarlet-I-Myl9 (MYL9 Human)
    

    Use

    Lentiviral

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha (with UCOE) and none (same transcript as…

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AAV-hSyn-Ribo-jGCaMP8f

    Plasmid

    #167573

    Purpose

    DEPRECATED: Please see Addgene item #169249 (coming soon) for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    Ribo-GCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-CBA-DIO-hM4Di-mCherry

    Plasmid

    #81008

    Purpose

    Cre-dependent expression of hM4Di-mCherry under CBA promoter

    Depositor

    Bernardo Sabatini

    Article

    Hou et al Cell. 2016 Sep 22;167(1):73-86.e12. doi:

    Insert

    hM4D(Gi)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    CBA

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MSCV-PIG-empty

    Plasmid

    #105594

    Purpose

    empty control vector with GFP marker and puro resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    no insert, empty control vector
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV-LTR

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJJiaDEST-APEX2

    Plasmid

    #149517

    Purpose

    N-termial APEX2 tag. Gateway destination vector for mammanlian expression

    Depositor

    Vojo Deretic

    Article

    Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

    Type

    Empty backbone

    Use

    Tags

    FLAG-APEX2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. GAL-Aur1-2GFP-Hygromycin

    Plasmid

    #184775

    Purpose

    Yeast expression of Aur1 under GAL promoter. Uses antibiotic resistance marker hphMX6.

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    proGAL-Aur1 (AUR1 Budding Yeast)
    

    Use

    Tags

    2EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDV17_pCMV-MCP-ADAR2dd(E488Q)-NES

    Plasmid

    #200406

    Purpose

    Expresses a compact, engineered version of ADAR2 constitutively in mammalian cells

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    MCP-ADAR2dd(E488Q)-NES
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    E488Q (hyperactive)

    Promoter

    CMV

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pLenti-STAT3-linker-TagGFP2

    Plasmid

    #121427

    Purpose

    WT STAT3 inserted using Infusion Cloning into MluI and EcoRI digested pLenti-Origene-Nrf21.

    Depositor

    Ovijit Chaudhuri, Stanley Qi

    Article

    Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

    Insert

    STAT3-linker-TagGFP2
    

    Use

    Tags

    TagGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. SaCas9_sgRNA_expression_in_pBluescript

    Plasmid

    #122090

    Purpose

    U6 driven SaCas9 sgRNA expression vector for cloning own guides

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SaCas9 tracr (NEWENTRY S. pyogenes, Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. NET47 pEGFP-N2 (593)

    Plasmid

    #61986

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET47 (TM7SF2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

37. BiP 2: C. elegans BiP (hsp-4)-GFP

    Plasmid

    #21896

    Depositor

    David Ron

    Article

    Calfon et al Nature. 2002 Jan 3. 415(6867):92-6.

    Insert

    BiP (hsp-4 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Fusion between the GFP and hsp-4 takes place at A…

    Promoter

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. DCIP

    Plasmid

    #193860

    Purpose

    Encodes SV40-mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    NLS-mCherry-GFP1-10
    

    Use

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCDH-MND-OsTIR1(F74G)-P2A-EGFP-AID2-EF1a-RFP

    Plasmid

    #232800

    Purpose

    Helper plasmid to trigger auxin-inducible-degradation of targeted proteins.

    Depositor

    Chunliang Li

    Article

    Hyle et al Genome Biol. 2023 Jan 26;24(1):14. doi:

    Insert

    OsTIR1 F74G EGFP-AID
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. QUAS-5-T7-TetO-GFP_ColE1

    Plasmid

    #171660

    Purpose

    QUAS five base pairs upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli according to the Q and Tet systems. Contains the ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    QUAS-5-T7-TetO-GFP ssra-T7 Stop
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAV U6+27

    Plasmid

    #25709

    Purpose

    for nuclear expression of small RNAs in mammalian cells

    Depositor

    David Engelke

    Article

    Good et al Gene Ther. 1997 Jan . 4(1):45-54.

    Insert

    U6 snRNP (LSM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pEGFP-mAV

    Plasmid

    #129472

    Purpose

    Mammalian expression of full-length mouse archvillin (Supervillin, isoform 2)

    Depositor

    Elizabeth Luna

    Article

    Oh et al J Cell Sci. 2003 Jun 1. 116(Pt 11):2261-

    Insert

    Supervillin, isoform 2 (Svil Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. peGFP(N1)-deltaCMV-rSyntaxin1a-meGFP

    Plasmid

    #34631

    Depositor

    Wolfhard Almers

    Article

    Barg et al Proc Natl Acad Sci U S A. 2010 Nov 30;10

    Insert

    rat Syntaxin1a (Stx1a Rat)
    

    Use

    Tags

    meGFP

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Feb. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. Human SERCA3 (pMT2)

    Plasmid

    #75189

    Purpose

    Mammalian expression of ATP2A3

    Depositor

    David MacLennan, Jonathan Lytton

    Article

    Poch et al Am J Physiol. 1998 Dec;275(6 Pt 1):C1449

    Insert

    ATP2A3 (ATP2A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    adenovirus major late promoter

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. HisTEV-cDAXX

    Plasmid

    #197012

    Purpose

    E. coli expression vector

    Depositor

    Tanja Mittag

    Article

    Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

    Insert

    DAXX
    

    Use

    Tags

    6His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCAG-Myc-Oct3/4-IP

    Plasmid

    #13460

    Depositor

    Shinya Yamanaka

    Article

    Maruyama et al J Biol Chem. 2005 Jul 1. 280(26):24371-9

    Insert

    Oct3/4 (Pou5f1 Mouse)
    

    Use

    Tags

    Myc (6x)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pT7-EGFP-C1-HsRRP12_K

    Plasmid

    #146766

    Purpose

    Mammalian Expression of HsRRP12

    Depositor

    Elisa Izaurralde

    Article

    Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

    Insert

    HsRRP12 (RRP12 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pAAV.TBG mCherry-LDTS-LIRmut

    Plasmid

    #189003

    Purpose

    lipophagy-inducing adaptor protein, AAV, negative control

    Depositor

    Atsushi Hoshino

    Article

    Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

    Insert

    LDTS-LIRmut, mCherry
    

    Use

    AAV

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCFJ1149 - intron 3 smu-2 PATCs

    Plasmid

    #161516

    Purpose

    PATC-rich intron number three from smu-2 to stimulate germline expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron 3 from smu-2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCMV MN (eGFP)

    Plasmid

    #89932

    Purpose

    Expresses the MN fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.

    Depositor

    Carl Novina

    Article

    Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

    Insert

    MN
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    M.SssI residues 2-272 fragment

    Promoter

    CMV

    Available since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge