166,818 results

1. HA-ERalpha

   Plasmid

   #215093

   Purpose

   Expresses HA-ERalpha in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   ESR1 (ESR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. XPO6-EGFP

   Plasmid

   #210371

   Purpose

   Expresses XPO6 fused with EGFP in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   XPO6 (XPO6 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. dCas9-10xSunTag-P2A-tagBFP

   Plasmid

   #174141

   Purpose

   Expresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.

   Depositor

   Albert Jeltsch

   Article

   Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

   Insert

   dCas9, 10xSunTag, tagBFP
   

   Use

   Tags

   HA and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. CIp10 - γmGFP (γmGFP based)

   Plasmid

   #163119

   Purpose

   Expresses in Candida albicans

   Depositor

   Patrick Van Dijck

   Article

   Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

   Insert

   gamma mGFP
   

   Use

   Candida albicans expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-DsRed2

   Plasmid

   #15777

   Depositor

   Edward Callaway

   Article

   Wickersham et al Neuron. 2007 Mar 1. 53(5):639-47.

   Insert

   DsRed2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA6-N-3XFLAG-Ctnnb1

   Plasmid

   #123586

   Depositor

   Bruce Beutler

   Article

   Choi et al Science. 2019 May 10;364(6440). pii: 364

   Insert

   catenin (cadherin associated protein), beta 1 (Ctnnb1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. 2C-EGFP

   Plasmid

   #69071

   Purpose

   Expresses EGFP under MERVL_LTR

   Depositor

   Maria-Elena Torres-Padilla

   Article

   Ishiuchi et al Nat Struct Mol Biol. 2015 Aug 3. doi: 10

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMSCVpuro-PCAF

   Plasmid

   #63705

   Purpose

   retroviral expression of human PCAF in mammalian cells

   Depositor

   Kai Ge

   Article

   Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

   Insert

   PCAF (KAT2B Human)
   

   Use

   Retroviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   E452G

   Promoter

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-EF1A-CD19.8h.8TM.28.3z

   Plasmid

   #200670

   Purpose

   Modular CD8hinge-CD8transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Michele Bernasconi

   Article

   Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

   Insert

   FMC63-CD8HD-CD8TM-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247 Human)
   

   Use

   Lentiviral

   Tags

   MycTag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-short

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Str-KDEL_neomycin

    Plasmid

    #65306

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
    

    Use

    Tags

    KDEL motif and Signal Sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-TO-dCas9-GFP

    Plasmid

    #64106

    Purpose

    Expression of Sp dCas9-GFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. MCP/PCP RNA Lantern

    Plasmid

    #226095

    Purpose

    RNA tracking

    Depositor

    Jennifer Prescher

    Article

    Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

    Insert

    NLS-HA-MS2-SmBiT-IRES-PP7-LgBiT-FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-TRE-DIO-hM3Dq-mCherry

    Plasmid

    #166599

    Purpose

    Encodes Cre-dependent hM3Dq-mCherry under control of the TRE

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    hM3Dq-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    tetracycline response element

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid

    #73796

    Purpose

    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA

    Depositor

    Ronald Davis

    Article

    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1

    Inserts

    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    

    Use

    CRISPR

    Tags

    Mxi1 and NLS

    Expression

    Yeast

    Mutation

    D10A, H840A

    Promoter

    pGPM1, pRPR1(TetO), and pTef1

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHS2 (hANGPTL4-V5)

    Plasmid

    #102446

    Purpose

    Expresses V5-tagged human ANGPTL4 in mammalian cells

    Depositor

    Brandon Davies

    Article

    Chi et al J Biol Chem. 2015 May 8;290(19):11865-77

    Insert

    ANGPTL4 (ANGPTL4 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMV306DIhsp+LuxG13

    Plasmid

    #49999

    Purpose

    Integrase removed to increase stability of the plasmid in mycobacteria

    Depositor

    Brian Robertson

    Article

    Andreu et al J Antimicrob Chemother. 2013 Sep;68(9):2

    Insert

    Bacterial luciferase operon + G13 promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    It contains a gram-positive enhanced translation …

    Promoter

    G13 from M. marinum G13 promoter driving luxC

    Available Since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. DDX5 WT V5

    Plasmid

    #199576

    Purpose

    Expresses WT DDX5 cloned into pLenti puro

    Depositor

    Michael Kastan

    Article

    McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKSI-1

    Plasmid

    #51725

    Purpose

    cloning backbone

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-Flag-RNF168

    Plasmid

    #133976

    Purpose

    mammalian expression vector of Flag tagged wild type RNF168

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 4, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pcDNA3-myc3-NEDD8

    Plasmid

    #19943

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2000 Nov . 20(21):8185-97

    Insert

    NEDD 8 (Neural precursor cell Expressed, Developmentally Down-regulated 8 (NEDD8 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

21. piggyBAC-U6-GFP-WPRE

    Plasmid

    #200030

    Purpose

    piggyBAC vector backbone with GFP for cloning DNA Ticker Tapes and U6 for pegRNAs

    Depositor

    Jay Shendure

    Type

    Empty backbone

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCCLc-MND-A0201-Mart1-SABR

    Plasmid

    #119052

    Purpose

    3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the Mart1 epitope "ELAGIGILTV" cloned via BsmBI

    Depositor

    David Baltimore

    Article

    Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

    Insert

    A0201-Mart1-SABR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MND

    Available Since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pHM129

    Plasmid

    #225534

    Purpose

    Encodes destablized mCherry under AR8 promoter, and constitutive BFP

    Depositor

    Jared Toettcher

    Article

    McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

    Inserts

    mCherry
    BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pYZ463

    Plasmid

    #187971

    Purpose

    TEF1p-Cas9-CYC1t and SNR52p-Not1-SUP4t

    Depositor

    Jef Boeke

    Article

    Zhao et al Nucleic Acids Res. 2023 Jun 16:gkad491.

    Insert

    Cas9 (codon optimized for human)
    

    Use

    Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-hSYN1-fDIO-tTA

    Plasmid

    #166597

    Purpose

    Encodes Flp-dependent tTA

    Depositor

    Martin Myers

    Article

    Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

    Insert

    tTA
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human synapsin 1

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. GFP-TREX1(D18N)

    Plasmid

    #27220

    Depositor

    Judy Lieberman

    Article

    Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

    Insert

    TREX1 (TREX1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    D18N sequence 52-GAC changed to AAC, resulting i…

    Promoter

    Available Since

    Jan. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HA WDR24 pRK5

    Plasmid

    #46335

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    WDR24 (WDR24 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEGB 35S:Renilla:Tnos (GB0109)

    Plasmid

    #68215

    Purpose

    Transcriptional unit for Renilla Luciferase Protein plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Renilla
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTXB1-CfaC-Gyra

    Plasmid

    #122950

    Purpose

    Bacterial expression of C-intein

    Depositor

    Thomas Muir

    Article

    Stevens et al J Am Chem Soc. 2016 Feb 24;138(7):2162-5

    Insert

    CfaC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Lenti-Hb9-RFP

    Plasmid

    #37081

    Depositor

    Fred Gage

    Article

    Marchetto et al Cell Stem Cell. 2008 Dec 4;3(6):649-57.

    Insert

    Hb9 RFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Hb9

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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