We narrowed to 16,513 results for: IGH@

1. pRL_yEGFP_hc

   Plasmid

   #98746

   Purpose

   Reporter plasmid for the PhiReX system. Encodes the reporter gene yEGFP under the control of the CYC1 minimal promoter with upstream synTALE-DBS.

   Depositor

   Bernd Müller-Röber

   Article

   Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919

   Insert

   Yeast-enhanced green fluorescent protein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   synthetic

   Available since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-attBr-Cre-rc-attP

   Plasmid

   #51264

   Purpose

   Component of the Bxb1-rcCre binary site-specific recombinase system, reverse complementary Cre CDS flanked by Bxb1 attB/P recognition sites in head-to-head orientation

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   attBr-Cre-rc-attP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pOVC1_EcSSPB-nano_X

   Plasmid

   #131212

   Purpose

   Expresses EcSSPB-nano (Escherichia coli) followed by restriction sites

   Depositor

   Harald Janovjak

   Article

   Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

   Insert

   EcSSPB-nano
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mc2 mCherry

   Plasmid

   #206219

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYPQ-dpcoCas9-EV2.1

   Plasmid

   #158411

   Purpose

   CRISPR-dpcoCas9 fused with EDLL activator for transcriptional activation

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dpcoCas9-EDLL-T2A-MS2-VPR(VP64-p65-Rta)-NOS-ter
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MXS_CMV::CreERT2-bGHpA

   Plasmid

   #62443

   Purpose

   MXS_chaining vector with CMV::CreERT2-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   cassette containing the tamoxifen inducible Cre recombinase with CMV Promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Nano-lantern(Ca2+)_300/pcDNA3

   Plasmid

   #51983

   Purpose

   mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nM

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_CaM_E104Q-3GS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAG260

   Plasmid

   #33210

   Depositor

   David Bartel

   Article

   Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

   Insert

   SYNM (SYNM Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   SV40 early enhancer/promoter

   Available since

   Jan. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pJK03_Rho_basal_DsRed

   Plasmid

   #173490

   Purpose

   Vector backbone used to clone MPRA libraries

   Depositor

   Barak Cohen

   Article

   White et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

   Insert

   Bovine Rhodopsin promoter driving DsRed fluorescent protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Bovine Rhodopsin

   Available since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDGE336

    Plasmid

    #153245

    Purpose

    M4 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDGE341

    Plasmid

    #153250

    Purpose

    M7 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AB.pCCL.sin.cPPT.GFP.miR-185-5p.sensor.PGK.dNGFR.WPRE

    Plasmid

    #85930

    Purpose

    Sensor Plasmid

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-185-5p Sensor (MIR185 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mRuby2-MannII-N-10

    Plasmid

    #55903

    Purpose

    Localization: Mannosidase/Golgi, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    MannII
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    aa 1-112 of MannII

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. IDP466

    Plasmid

    #87008

    Purpose

    genomic Sindbis virus plasmid for expression of SYNseq components

    Depositor

    Anthony Zador

    Article

    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

    Inserts

    myc-3xFlag-CLIP-Nrx1B-1xλN(i)
    eGFP
    

    Use

    Synthetic Biology; Sindbis virus genomic plasmid

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 907H1

    Plasmid

    #160234

    Purpose

    FlightN-CymR-VP16-3X-SV40-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    p-cymene repressor
    Flightin promoter
    

    Use

    Tags

    VP16

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MXS_CMV::NeoR-bGHpA

    Plasmid

    #62437

    Purpose

    MXS_chaining vector with CMV::NeoR-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    resistance cassette against Neomycin with CMV Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pgRNA-guideless

    Plasmid

    #129464

    Purpose

    Derived from pSCB2-sgRNA. 20 nucleotide binding sequence of sgRNA removed by inverse PCR. Used as a negative control.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Guideless gRNA backbone
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 (SpeI)

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pASKt15C+SrtA5

    Plasmid

    #64985

    Purpose

    Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    His6 tag and S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, P94R, D160N, D165A, K19…

    Promoter

    tetracycline promoter/operator

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCLR2070

    Plasmid

    #28063

    Depositor

    Keith Joung

    Article

    Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

    Insert

    FokI endonuclease
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains N496D point mutation in FokI domain, as …

    Promoter

    Available since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCLR2068

    Plasmid

    #28062

    Depositor

    Keith Joung

    Article

    Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

    Insert

    FokI endonuclease
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains H537R point mutation in FokI domain, as …

    Promoter

    Available since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Dendra2-Keratin-17

    Plasmid

    #57726

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Keratin (KRT18 Human)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

22. pET-DUET-1-mRhubarb719-HO

    Plasmid

    #141200

    Purpose

    Expresses mRhubarb719 fluorescent protein and heme oxygenase

    Depositor

    Elad Firnberg

    Article

    Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

    Inserts

    mRhubarb719
    heme oxygenase
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

    Promoter

    T7

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mc2 neo

    Plasmid

    #206214

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Salsa-BC82-P1G10

    Plasmid

    #202923

    Purpose

    Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC82

    Depositor

    Noah Palm

    Article

    Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

    Insert

    EGFP/BC82
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet Response Element (TRE)

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGEX6p1 AO7RE

    Plasmid

    #137843

    Purpose

    GST tagged AO7 encoding aa 126-258 for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEX-4T-1-p19-T7-PLK1

    Plasmid

    #46312

    Depositor

    Judy Lieberman

    Article

    Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

    Inserts

    p19
    PLK1 DNA-1
    linker
    PLK1 DNA-2
    

    Use

    Pro-sirna

    Tags

    6XHis and GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

27. Salsa-BC2-P1A2

    Plasmid

    #202843

    Purpose

    Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC2

    Depositor

    Noah Palm

    Article

    Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

    Insert

    EGFP/BC2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet Response Element (TRE)

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDS272

    Plasmid

    #34975

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2-GFP-LOVpep (MID2 Budding Yeast, A. sativa)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T406A, T407A

    Promoter

    TEF

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEMHE-mClover3

    Plasmid

    #171497

    Purpose

    T7 promotor drives in vitro transcription of mClover3 mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    mClover3
    

    Use

    Pgehme

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMX-PGC1

    Plasmid

    #6

    Depositor

    Bruce Spiegelman

    Article

    Puigserver et al Cell 1998 Mar 20;92(6):829-39.

    Insert

    PGC-1 alpha (Ppargc1a Mouse)
    

    Use

    Tags

    Gal4

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2005

    Availability

    Academic Institutions and Nonprofits only

31. FLAG TBC1D13

    Plasmid

    #87953

    Purpose

    Expresses flag tagged mouse TBC1D13

    Depositor

    David James

    Article

    Davey et al Traffic. 2012 Oct;13(10):1429-41. doi: 1

    Insert

    TBC1D13 (Tbc1d13 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pDGE508

    Plasmid

    #153280

    Purpose

    End-linker for cloning 2 sgRNA arrays into any recipient vector

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Insert

    linker
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. IDP425

    Plasmid

    #87022

    Purpose

    genomic Sindbis virus plasmid for use in SYNseq PLA screen

    Depositor

    Anthony Zador

    Article

    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

    Inserts

    SNAP-Nlg1AB-1xλN(i)
    mCherry
    

    Use

    Synthetic Biology; Sindbis virus genomic plasmid

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. mNG-mTq2

    Plasmid

    #124217

    Purpose

    Cytoplasmic mNeongreen mTurquoise2 tandem, ~60 % energy transfer. pNM043 - pSAV057-mNG-mTq2

    Depositor

    Tanneke den Blaauwen

    Article

    Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

    Insert

    mNG-mTq2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Nano-lantern(Ca2+)_600/pcDNA3

    Plasmid

    #51982

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=600nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-2G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

36. pHH0103_UEVLD_UEV

    Plasmid

    #109902

    Purpose

    Protein expression and purification of UEVLD_UEV

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    UEVLD_UEV (UEVLD Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHW-CIBN-MP-HRW-CRY2-VHH(GFP)

    Plasmid

    #126024

    Purpose

    express RFP-tagged LARIAT for optogenetic clustering

    Depositor

    Eurico Morais-de-Sá

    Article

    Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

    Insert

    CIBN-MP; CRY2 PHR-VHH(GFP)
    

    Use

    Tags

    RFP

    Expression

    Insect

    Mutation

    WT

    Promoter

    Hsp70

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. jk107

    Plasmid

    #87007

    Purpose

    plasmid for cell culture expression of SYNseq components

    Depositor

    Anthony Zador

    Article

    Peikon et al Nucleic Acids Res. 2017 Jul 7;45(12):e11

    Inserts

    SNAP-Nlg1AB-1xλN(i)
    mCherry
    

    Use

    Synthetic Biology; Sindbis virus genomic plasmid

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Salsa-BC6-P1A6

    Plasmid

    #202847

    Purpose

    Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC6

    Depositor

    Noah Palm

    Article

    Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

    Insert

    EGFP/BC6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet Response Element (TRE)

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAGM7547

    Plasmid

    #169091

    Purpose

    MoClo level 1 35S promoter-Db5GT-35S terminator transcription unit

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

    Insert

    35S promoter-Db5GT-35S terminator
    

    Use

    Moclo cloning vector

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAGM7523

    Plasmid

    #169090

    Purpose

    MoClo level 1 35S promoter-BvDODA1-Ocs terminator transcription unit

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

    Insert

    35S promoter-BvDODA1-Ocs terminator
    

    Use

    Moclo cloning vector

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pAGM7535

    Plasmid

    #169089

    Purpose

    MoClo level 1 35S promoter-BvCYP76AD1-Nos terminator transcription unit

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

    Insert

    35S promoter-BvCYP76AD1-Nos terminator
    

    Use

    Moclo cloning vector

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. miniSOG-Lifeact-7

    Plasmid

    #54823

    Purpose

    Localization: Actin, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Type

    Empty backbone

    Use

    Tags

    Lifeact and miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

44. pNS3

    Plasmid

    #131770

    Purpose

    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites

    Depositor

    Jared Toettcher

    Article

    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

    Insert

    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1
    

    Use

    Integration into δ-sites

    Tags

    Expression

    Yeast

    Mutation

    E490G to make Cry2 into Cry2Olig

    Promoter

    PADH1

    Available since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. Clover-Lysosomes-20

    Plasmid

    #56528

    Purpose

    Localization: Lysosome Membrane, Excitation: 505, Emission: 515

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    LAMP1
    

    Use

    Tags

    Clover

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

46. pNS1

    Plasmid

    #131769

    Purpose

    PADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sites

    Depositor

    Jared Toettcher

    Article

    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

    Insert

    FUSN_Fusion Red_Cry2WT
    

    Use

    Integration into δ-sites

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PADH1

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pHH0103_SIAH2_Sina

    Plasmid

    #109813

    Purpose

    Protein expression and purification of SIAH2_Sina

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    SIAH2_Sina (SIAH2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. TAL2274

    Plasmid

    #36711

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    MDM2-TALEN_Left (Mdm2 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

49. pCellFree_G08

    Plasmid

    #67142

    Purpose

    Gateway destination vector for cell-free expression with C-term mCherry myc-tag tag

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

    Type

    Empty backbone

    Use

    Cell-free expression

    Tags

    His-MYC and mCherry

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. AB.pCCL.sin.cPPT.GFP.miR-21-3p.sensor.PGK.dNGFR.WPRE

    Plasmid

    #85857

    Purpose

    Sensor Plasmid

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-21-3p Sensor (MIR21 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge