We narrowed to 16,910 results for: igh

1. pCAG-b3STOPb3-ZsGreen

   Plasmid

   #51266

   Purpose

   B3-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by B3-recognition sites followed by ZsGreen CDS

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   b3STOPb3-ZsGreen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. env8 reverse J1/13

   Plasmid

   #99831

   Purpose

   GFPuv reporter that places expression of a fluorescent protein reporter (GFPuv) under control of the env8HyCbl riboswitch.

   Depositor

   Robert Batey

   Article

   Polaski et al Cell Rep. 2016 May 3;15(5):1100-10. doi:

   Insert

   env8 reverse J1/13
   

   Use

   Tags

   GFPuv

   Expression

   Bacterial

   Mutation

   J1/13 sequence is reversed

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKIR1.0

   Plasmid

   #86409

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Expression

   Mutation

   Promoter

   AtRPS5A

   Available since

   Feb. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. FRP792_insul-(lexA-box)2-PminCYC1-Citrine-TCYC1

   Plasmid

   #58433

   Purpose

   Induces Citrine expression from a promoter containing 2 lexA boxes in yeast cells

   Depositor

   Joerg Stelling

   Article

   Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

   Insert

   CitrineA206K
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   insul-(lexA-box)2-PminCYC1

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRSFDuet-1_mNeongreen

   Plasmid

   #182413

   Purpose

   Bacterial expression of mNeongreen

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   mNeongreen
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPAmCherry1-N1

   Plasmid

   #31928

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

   Insert

   PAmCherry1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

   Promoter

   cmv

   Available since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBR_attB(bxb)_ccdB_lox_v2

   Plasmid

   #198040

   Purpose

   Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. eNme2C-CBE6b

   Plasmid

   #215830

   Purpose

   Express CBE6b (with eNme2-C variant) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6b-eNme2C-2xUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNCS-mRuby3

   Plasmid

   #74234

   Purpose

   Constitutive expression of mRuby3 in bacteria

   Depositor

   Michael Lin

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Insert

   mRuby3
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSBtet-Hygro-GADD34_Δ1-240/P2A/K3L

    Plasmid

    #196136

    Purpose

    SB vector for the mammalian expression of GADD34-K3L

    Depositor

    Jamie Cate

    Article

    Aleksashin et al bioRxiv. 2023 Feb 10:2023.02.09.527910.

    Insert

    GADD34 Δ1-240 - P2A - K3L (PPP1R15A Human, vaccinia virus)
    

    Use

    Tags

    P2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1+-mutTornado-split-nLuc

    Plasmid

    #212707

    Purpose

    Tornado split nLuc with a mutated 3' ribozyme so that circularization cannot occur.

    Depositor

    Samie Jaffrey

    Article

    Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

    Insert

    mutTornado-CVB3-split-nLuc
    

    Use

    Tags

    Expression

    Mutation

    Deleted the partial 3'ribozyme sequence in T…

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDest-635

    Plasmid

    #159582

    Purpose

    Gateway Destination vector for protein expression in baculovirus

    Depositor

    Dominic Esposito

    Article

    Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. paavCAG-Jx-rev-post-mGRASP-2A-dTomato

    Plasmid

    #34913

    Purpose

    JxON-post

    Depositor

    Jinhyun Kim

    Article

    Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

    Insert

    postsynaptic mGRASP
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Nov. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. human syndecan 2 wt

    Plasmid

    #64970

    Purpose

    Constitutive expression in mammalian cells

    Depositor

    Enric Espel

    Article

    Rovira-Clave et al Cell Signal. 2014 Jul;26(7):1489-99. doi

    Insert

    syndecan 2 (SDC2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

15. IFI16-FL-(1-729)

    Plasmid

    #35051

    Depositor

    Cheryl Arrowsmith

    Article

    Liao et al Structure. 2011 Mar 9;19(3):418-29.

    Insert

    IFI16 (IFI16 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 6, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pEGFP-SF2

    Plasmid

    #17990

    Depositor

    Tom Misteli

    Article

    Phair et al Nature. 2000 Apr 6. 404(6778):604-9.

    Insert

    SF2 (SRSF1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains SF2 (aa1-210)

    Promoter

    Available since

    May 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mTagBFP2-SiT-N-15

    Plasmid

    #55325

    Purpose

    Localization: Golgi TGN, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    SiT (ST6GAL1 Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    aa 1-45 of SiT

    Promoter

    CMV

    Available since

    Oct. 24, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

18. Rab5a-pmCherryC1

    Plasmid

    #27679

    Depositor

    Christien Merrifield

    Article

    Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

    Insert

    Rab5a (Rab5a Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYPQ142-ZmUbi-tRNA

    Plasmid

    #158578

    Purpose

    Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 and pYPQ132-tRNA2.0; assembly of 2 gRNAs

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin 1

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWRS_1001

    Plasmid

    #192205

    Purpose

    lentiviral expression of SpCas9 and two pol III promoters for dual expression of sgRNAs

    Depositor

    William Sellers

    Article

    Li et al Nat Commun. 2022 May 5;13(1):2469. doi:

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. miniSOG-N1

    Plasmid

    #54824

    Purpose

    Localization: N1 Cloning Vector, Excitation: 448 / 473, Emission: 500 / 528

    Depositor

    Michael Davidson

    Article

    Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

    Type

    Empty backbone

    Use

    Tags

    miniSOG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pLN-AIDA-Tyr1

    Plasmid

    #192831

    Purpose

    IPTG inducible AIDA-Tyrosinase expression

    Depositor

    André Studart

    Article

    Binelli et al Adv Mater. 2022 Nov 29:e2207483. doi: 10

    Insert

    AIDA-Tyr1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pL-lac

    Available since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET24b-Amg2

    Plasmid

    #199434

    Purpose

    expresses Amg2 in bacterial cells

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    Amg2
    

    Use

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    L159K mutation

    Promoter

    T7

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pDNR-mCherry-T2A-3xPuroR-Frame 0

    Plasmid

    #206991

    Purpose

    Donor plasmid for Frame 0 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

    Depositor

    Alejandro Chavez

    Article

    Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Bxb1_tdTomato

    Plasmid

    #198041

    Purpose

    Bxb1-specific donor plasmid for integrating a transcription unit for constitutive expression of tdTomato reporter.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

    Insert

    tdTomato
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pERB215

    Plasmid

    #61499

    Purpose

    Const. Halotag-GFP-Nuf2 + mCherry-DHFR

    Depositor

    Michael Lampson, David Chenoweth

    Article

    Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

    Inserts

    HaloTag-GFP-Nuf2
    mCherry-DHFR
    

    Use

    Tags

    HaloTag and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1-Tat101(AD8)-Flag

    Plasmid

    #115777

    Purpose

    pCMV-Tat101AD8-Flag allows the expression of Tat101(AD8) protein with a C-terminal flag-tag.

    Depositor

    Damian Purcell

    Article

    Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

    Insert

    HIV-1 Tat protein (AD8)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. paavCAG-pre-mGRASP-mCerulean-2A-nls-mCherry

    Plasmid

    #34911

    Depositor

    Jinhyun Kim

    Article

    Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

    Insert

    presynaptic mGRASP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    Nov. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMK393 (mAID-mCherry-BSD)

    Plasmid

    #121194

    Purpose

    C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    mAID-mCherry-BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. His SUMO TEV SPOP 1-374

    Plasmid

    #197015

    Purpose

    E. coli expression vector

    Depositor

    Tanja Mittag

    Article

    Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

    Insert

    SPOP (Spop Mouse)
    

    Use

    Tags

    6His-SUMO-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMUT2-pFF-41.5-mCherry

    Plasmid

    #192863

    Purpose

    pMUT2 based vector for sensing oxygen in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    Oxygen inducible promoter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28b-irp3 WT

    Plasmid

    #87106

    Purpose

    expresses Irp3 WT, the thiazolinyl imine reductase from Yersinia enterocolitica

    Depositor

    Audrey Lamb

    Article

    Meneely et al Biochemistry. 2012 Nov 6;51(44):9002-13.

    Insert

    irp3 WT
    

    Use

    Tags

    his-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCHW110033

    Plasmid

    #222441

    Purpose

    Level 0, CDS module (AATG-GCTT) containing Cre recombinase.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    Cre recombinase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMDISI

    Plasmid

    #51653

    Purpose

    E coli genetic modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    spcR
    

    Use

    Genetic modification

    Tags

    Expression

    Mutation

    Promoter

    unknown

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET21a - NR

    Plasmid

    #89625

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi nitrate reductase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Nitrate reductase
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pPAmCherry1-C1

    Plasmid

    #31929

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E26V/A58T/K69N/L84F/N99K/S148L/I165V/Q167P/L169V/…

    Promoter

    CMV

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCHW110035

    Plasmid

    #222444

    Purpose

    Level 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry-stop.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    LoxP-mCherry-stop
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    no

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBbAk-w4-loxP-TT-loxP-mCherry-PCB

    Plasmid

    #228469

    Purpose

    Fluorescent reporter for Cre recombinase activity and the PCB production gene. Cre removes a transcription terminator, leading to mCherry production

    Depositor

    Mary Dunlop

    Article

    Jafarbeglou et al ACS Synth Biol. 2024 Nov 19. doi: 10.102

    Inserts

    w4-loxP-TT-loxP-mCherry
    Ho1
    PcyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23106, J23108, and T7A1

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1-Cry2FL-VP64

    Plasmid

    #60554

    Purpose

    Expresses Cry2FL-VP64 in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    Cry2FL-VP64
    

    Use

    Tags

    FLAG, HA tag, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. mNeonGreen-mRuby2-FRET-10

    Plasmid

    #58179

    Purpose

    Localization: Cytosolic FRET, Excitation: , Emission:

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2013 May;10(5):407-9. doi:

    Type

    Empty backbone

    Use

    Tags

    mNeonGreen and mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge