We narrowed to 4,430 results for: Erf

1. pJBL-RNAINS4-A25C

   Plasmid

   #69461

   Purpose

   RNA-IN S4 A25C variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

   Depositor

   Julius Lucks

   Article

   Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

   Insert

   RNA-IN S4 with A25C mutation
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   A25C and mutations for S4 variant (from wt)

   Promoter

   J23119 and J23119 (sigma 70)

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJBL-RNAINS4-C24AA25C

   Plasmid

   #69463

   Purpose

   RNA-IN S4 C24AA25C variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

   Depositor

   Julius Lucks

   Article

   Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

   Insert

   RNA-IN S4 with C24A, A25C mutations
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   C24A, A25C, and mutations for S4 variant (from wt)

   Promoter

   J23119 and J23119 (sigma 70)

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-ybbR-HIS-Spec(x2)-DocI

   Plasmid

   #58713

   Purpose

   Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

   Depositor

   Hermann Gaub

   Article

   Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

   Insert

   Spec(x2)-DocI
   

   Use

   Tags

   HIS, HRV 3C, and ybbR

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX303-ZIM3-KRAB-dCas9

   Plasmid

   #154472

   Purpose

   Expresses ZIM3 KRAB domain fused to the N terminus of dCas9

   Depositor

   Mikko Taipale

   Article

   Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

   Insert

   ZIM3 (ZIM3 Synthetic)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Includes ZIM3 aa 1-100

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pdCRISPomyces-2BD

   Plasmid

   #234429

   Purpose

   Plasmid for Streptomyces containing gene of modified dCas9-BD protein and sequence of sgRNA cloning template

   Depositor

   Min-Kyu Oh

   Article

   Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:

   Insert

   Modified dCas9(D10A, H840A) with polyaspartate linking
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Linked polyaspartate using glycine-serine linker

   Promoter

   Available since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE TRE dCas9-KRAB

   Plasmid

   #50917

   Purpose

   Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression vector

   Depositor

   Rene Maehr, Scot Wolfe

   Article

   Kearns et al Development. 2014 Jan;141(1):219-23. doi

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and KRAB

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   TRE

   Available since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-hfYFP

   Plasmid

   #191201

   Purpose

   Constitutive CAG-driven expression of hfYFP in animals using AAV

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   Hyperfolder YFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

   Promoter

   CAG

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-TRE-dCas9-KRAB-MeCP2

   Plasmid

   #122267

   Purpose

   SP-dCas9-KRAB-MeCP2 with doxycycline-inducible expression

   Depositor

   Andrea Califano

   Article

   Tan et al bioRxiv 2021.06.28.449297

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   Tags

   dCas9-KRAB-MeCP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-dCas9-TurboID-blast

   Plasmid

   #222599

   Purpose

   Express fussed dCas9-TurboID for proximity labeling

   Depositor

   Yarui Diao

   Article

   Sun et al Nat Genet. 2023 Aug;55(8):1324-1335. doi

   Insert

   dCas9-TurboID
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF-1α

   Available since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE60 Q58L wgMDH

    Plasmid

    #204246

    Purpose

    Bacterial expression of Q58L wgMDH with IPTG induction - Subunit interface mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Q58L

    Promoter

    T5

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJAK184.tetR.PT5-3.dCas9

    Plasmid

    #232482

    Purpose

    optimized ahTet inducible dCas9 for insertion into C. difficile genome

    Depositor

    James Collins

    Article

    Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

    Insert

    Catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetR-PT5-3

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXPR_123

    Plasmid

    #96919

    Purpose

    for CRISPRi, lentiviral expression of dCas9-p300 2A BlastR.

    Depositor

    John Doench, David Root

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    p300

    Expression

    Mammalian

    Mutation

    dCas9

    Promoter

    EF1a

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. p-dCas9-SSAP-MS2-BB_BbsI

    Plasmid

    #183826

    Purpose

    pU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFP

    Depositor

    Le Cong

    Article

    Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

    Insert

    U6-guideRNA-dCas9-T2A-EBFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBH

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE EF1α dCas9-NED

    Plasmid

    #109369

    Purpose

    Lentiviral vector for expressing effector domains fused to dCas9.

    Depositor

    Antal Kiss

    Article

    Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9

    Plasmid

    #84245

    Purpose

    Expresses direct fusion KRAB-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    KRAB-tagBFP-Sp dCas9
    Puro
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), HA tag, KRAB, and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and PGK

    Available since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-tight-DDdCas9GFP-IRES-Neo

    Plasmid

    #102907

    Purpose

    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    TRE-tight

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKD237-3a-2

    Plasmid

    #90957

    Purpose

    Expresses ThsR and mCherry constitutively and sfGFP under the PphsA promoter

    Depositor

    Jeffrey Tabor

    Article

    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

    Inserts

    Thiosulfate response regulator
    Superfolder GFP
    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Bba_J23100, Bba_J23105, and PphsA

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJMP1356

    Plasmid

    #119276

    Purpose

    for cloning new sgRNAs

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpdCas9 KRAB

    Plasmid

    #188499

    Purpose

    Expresses FLAG tagged SpdCas9 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/H840A

    Promoter

    EF1a

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOSIP-KH-RBS2-dCas9

    Plasmid

    #182740

    Purpose

    Integrative plasmid at the HK022 site in E. coli carrying dCas9 under the control of a Ptet promoter

    Depositor

    David Bikard

    Article

    Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

    Insert

    dCas9
    

    Use

    CRISPR; Integrative vector

    Tags

    Expression

    Mutation

    Promoter

    Ptet

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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