We narrowed to 964 results for: Streptococcus

1. pJWV25

   Plasmid

   #61045

   Purpose

   To construct N-terminal GFP fusions under control of a Zn2+ inducible promoter, integrates via double crossover at bgaA

   Depositor

   Jan-Willem Veening

   Article

   Eberhardt et al Mol Microbiol. 2009 Oct;74(2):395-408. d

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   GFP+

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. cr3Ec

   Plasmid

   #184860

   Purpose

   cr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and an E.coli-specific recombination system

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   Cas9, gam, bet, exo
   

   Use

   Tags

   Cas9 ssrA degradation tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRU53

   Plasmid

   #167679

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pspCas9-3'UTR-ST2-com-vector

   Plasmid

   #136269

   Purpose

   Expresses SpCas9 and sgRNA Scaffold

   Depositor

   Baisong Lu

   Article

   Lu et al CRISPR J. 2021 Mar 16. doi: 10.1089/cris

   Insert

   SpCas9 and sgRNA scaffold
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRU54

   Plasmid

   #167680

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-(BbsI)sgRNA_CAG-Cas9-bpA_EF1-TagRFP

   Plasmid

   #86987

   Purpose

   For cloning and expression of sgRNA together with expression of Cas9 and TagRFP

   Depositor

   Ralf Kuehn

   Article

   Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRU293

   Plasmid

   #167688

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   pEC1.2::Cas9i
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMM178

   Plasmid

   #61270

   Purpose

   Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.

   Depositor

   Timothy Lu

   Article

   Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

   Insert

   Cas9, tracrRNA, crRNA
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO, native, native (respectively)

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX165-Sniper-Cas9

   Plasmid

   #140560

   Purpose

   Expresses Sniper Cas9 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Mutation

   F539S M763I K890N

   Promoter

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX165-Cas9-HF1

    Plasmid

    #140565

    Purpose

    Expresses Cas9-HF1 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mutation

    N497A R661A Q695A Q926A

    Promoter

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-CibN-dCas9

    Plasmid

    #60551

    Purpose

    Expresses CibN-dCas9 in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    CibN-dCas9
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    inactivating Cas9 mutations D10A and H840A

    Promoter

    CMV

    Available since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRU323

    Plasmid

    #167690

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUBQ4-2::Cas9i
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAE03

    Plasmid

    #61044

    Purpose

    To construct C-terminal GFP fusions, single crossover integration vector

    Depositor

    Jan-Willem Veening

    Article

    Eberhardt et al Mol Microbiol. 2009 Oct;74(2):395-408. d

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    GFP+

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRU292

    Plasmid

    #167687

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUBQ4-2::Cas9i
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSL690

    Plasmid

    #47753

    Purpose

    Expresses dCas9-VP64 fusion

    Depositor

    Keith Joung

    Article

    Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Insert

    dCas9-VP64
    

    Use

    CRISPR

    Tags

    3x FLAG, NLS, and VP64

    Expression

    Mammalian

    Mutation

    D10A and H840A mutations in Cas9

    Promoter

    CMV

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT3TS-HiFi-nCas9n-A72

    Plasmid

    #125646

    Purpose

    micro-injection of poly-adenylated HiFi-nCas9n RNA

    Depositor

    Jason Berman

    Article

    Prykhozhij et al Methods Mol Biol. 2020;2115:385-405. doi

    Insert

    HiFi-nCas9n
    

    Use

    Rna in vitro transcription vector

    Tags

    Expression

    Mutation

    R691A from wild-type Cas9

    Promoter

    T3

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRU52

    Plasmid

    #167678

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUB4-2::spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRU51

    Plasmid

    #167677

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUB4-2::spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX165-xCas9

    Plasmid

    #140568

    Purpose

    Expresses xCas9 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mutation

    A262T R324L S409I E480K E543D M694I E1219V

    Promoter

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21b - S. thermophilus DltB

    Plasmid

    #132607

    Purpose

    expresses S. thermophilus DltB in E. coli

    Depositor

    Wenqing Xu

    Article

    Ma et al Nature. 2018 Oct;562(7726):286-290. doi:

    Insert

    Dltb
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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