167,785 results

1. pcDNA3.1 3xFlag IFIT1

   Plasmid

   #53554

   Purpose

   mammalian expression of IFIT1

   Depositor

   Kathleen Collins

   Article

   Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

   Insert

   IFIT1 (IFIT1 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. Shank2-HA /pCDNA3

   Plasmid

   #197334

   Purpose

   Plasmid expressing an antigen targeting Shank2

   Depositor

   Morgan Sheng

   Insert

   Shank2 (Shank2 Rat)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   June 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-N-Blast-mStayGold

   Plasmid

   #227251

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mStayGold cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. hCRISPRi_dual_1_2

   Pooled Library

   #187246

   Purpose

   Ultra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Species

   Synthetic

   Use

   Lentiviral

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

5. pGEX4T-E2-25K

   Plasmid

   #63572

   Purpose

   Expression of human Ubiquitin conjugating enzyme E2-25K

   Depositor

   Titia Sixma

   Article

   Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

   Insert

   UBE2-25K (UBA7 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Isoform 1 (200 resiudes)

   Promoter

   tac

   Available Since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

6. PX552

   Plasmid

   #60958

   Purpose

   pAAV-U6sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). AAV plasmid for sgRNA cloning. GFP-KASH fusion facilitates FACS sorting of cells and nuclei.

   Depositor

   Feng Zhang

   Article

   Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

   Inserts

   U6_(SpaI)_sgRNA
   Syn_EGFP-KASH
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Expression

   Mammalian

   Promoter

   Synapsin and U6

   Available Since

   Nov. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. 0_T7-mScarlet3

   Plasmid

   #225927

   Purpose

   In vitro synthesis of mRNA for fluorescent protein mScarlet3 (control)

   Depositor

   Michalis Averof

   Article

   Karapidaki et al bioRxiv 2024.11.12.623055

   Insert

   mScarlet3
   

   Use

   In vitro transcription

   Promoter

   T7

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3XMEF2-luc

   Plasmid

   #32967

   Purpose

   Reporter gene for MEF2 activity with 3 MEF2 sites upstream of a c-fos minimal promoter

   Depositor

   Ron Prywes

   Insert

   MEF2 binding sites (MEF2A Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Promoter

   MEF2

   Available Since

   Feb. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSBFP2-C1

   Plasmid

   #22880

   Depositor

   Dorus Gadella

   Article

   Kremers et al Biochemistry. 2007 Mar 27. 46(12):3775-8

   Insert

   SBFP2
   

   Expression

   Mammalian

   Available Since

   Feb. 24, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-Cas9-GFP

    Plasmid

    #86145

    Purpose

    Lentiviral vector expressing Cas9-GFP

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    Cas9-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. ATF4 1: Mouse ATF4 (CHOP11/cATF)-WT

    Plasmid

    #21845

    Depositor

    David Ron

    Insert

    ATF4 (Atf4 Mouse)
    

    Expression

    Mammalian

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-Popt

    Plasmid

    #231705

    Purpose

    Mammalian expression vector encoding a codon-optimized P gene from vesicular stomatitis virus, strain Indiana.

    Depositor

    Ron Geller

    Article

    Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

    Insert

    VSV P gene (codon-optimized )
    

    Use

    Vsv expression

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-Lopt

    Plasmid

    #231706

    Purpose

    Mammalian expression vector encoding a codon-optimized L gene from vesicular stomatitis virus, strain Indiana.

    Depositor

    Ron Geller

    Article

    Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

    Insert

    VSV L gene (codon-optimized )
    

    Use

    Vsv expression

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. piRFP670-N1

    Plasmid

    #45457

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    iRFP670
    

    Expression

    Mammalian

    Mutation

    T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

    Promoter

    CMV

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-CMV-MCS-Puro-EGFP

    Plasmid

    #219795

    Purpose

    Mammalian expression plasmids using piggyBac Transposon System

    Depositor

    Keiichi Nakayama

    Type

    Empty backbone

    Use

    Piggybac transposon

    Expression

    Mammalian

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-C3-Sec5

    Plasmid

    #53756

    Purpose

    pEGFP-C3 plasmid that expresses Sec5

    Depositor

    Channing Der

    Article

    Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

    Insert

    Sec5 (EXOC2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pCDNA3.1-H-Luc_cyto

    Plasmid

    #113929

    Purpose

    Plasmid encoding the cytosolic localization of the chimeric HaloTag7-NanoLucCP fusion H-Luc in mammalian cells for BRET

    Depositor

    Kai Johnsson

    Article

    Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

    Insert

    H-Luc
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYMA3_SidK_GFP

    Plasmid

    #184276

    Purpose

    Legionella pneumophila SidK (1-278) - GFP

    Depositor

    John Rubinstein

    Article

    Maxson et al J Cell Biol. 2022 Mar 7;221(3). pii: 212

    Insert

    SidK (1-278) (sidK Legionella pneumophila)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV hUbC-dCas9-MQ1(Q147L)-EGFP

    Plasmid

    #89793

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR and Lentiviral

    Tags

    3*FLAG and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Q147L, CAA-->CTA

    Promoter

    hUbC

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-CaMPARI

    Plasmid

    #60421

    Purpose

    Expresses CaMPARI in mammalian cells, a photoconvertible fluorescent protein-based calcium integrator

    Depositor

    Eric Schreiter, Loren Looger

    Article

    Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

    Insert

    CaMPARI
    

    Tags

    Nuclear Export Signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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21. pP3-GFP

    Plasmid

    #53436

    Purpose

    Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

    Depositor

    Cynthia Collins

    Article

    Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

    Insert

    P3 promoter
    

    Use

    Synthetic Biology

    Tags

    none

    Expression

    Bacterial

    Mutation

    none

    Promoter

    P3

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. pNO286-3

    Plasmid

    #107746

    Purpose

    Key genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)

    Depositor

    Jeffrey Tabor

    Article

    Ong et al Chembiochem. 2018 Feb 8. doi: 10.1002/cb

    Inserts

    CcaS
    Ho1
    PcyA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Deleted AA 223 and 512

    Promoter

    J23106 and J23108

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pEGFP-C3-Sec15

    Plasmid

    #53760

    Purpose

    pEGFP-C3 plasmid that expresses Sec15

    Depositor

    Channing Der

    Article

    Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

    Insert

    Sec15 (EXOC6 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #113341

    Purpose

    Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    VP64-dCas9-VP64-T2A-MS2-p65-HSF1
    

    Use

    Piggybac

    Expression

    Mammalian

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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25. MoChlo: Modular Cloning Chloroplast Toolbox

    Plasmid Kit

    #1000000156

    Purpose

    The MoChlo Toolbox consists of a library of standardized chloroplast-specific genetic modules for Golden-Gate cloning of synthetic operons into chloroplast transformation vectors.

    Depositor

    Scott Lenaghan

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

26. EGFP-RILP

    Plasmid

    #110498

    Purpose

    Fluorescent RILP

    Depositor

    Bernardo Mainou, Terence Dermody

    Article

    Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

    Insert

    RILP (RILP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pJFRC19-13XLexAop2-IVS-myr::GFP

    Plasmid

    #26224

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    myr::GFP
    

    Expression

    Insect

    Available Since

    Oct. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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28. pET28-AviTag-DogCatcher-MBP

    Plasmid

    #171928

    Purpose

    Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    AviTag-DogCatcher-MBP
    

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    DogCatcher added to N-terminus of MBP via a GSGES…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. AAV2-hSyn-G-Flamp1

    Plasmid

    #188569

    Purpose

    green biosensor for cAMP in neurons

    Depositor

    Jun Chu

    Article

    Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

    Insert

    G-Flamp1
    

    Use

    AAV

    Tags

    His tag

    Promoter

    hSyn

    Available Since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pEGFP-Q23

    Plasmid

    #40261

    Depositor

    David Rubinsztein

    Article

    Narain et al J Med Genet. 1999 Oct;36(10):739-46.

    Insert

    HTT partial exon 1 Q23 (HTT Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    expanded poly-Q

    Promoter

    CMV

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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31. pDEST_FLAG-HUWE1

    Plasmid

    #187120

    Purpose

    mammalian cell expression of FLAG-HUWE1

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

    Insert

    HUWE1 (HUWE1 Human)
    

    Tags

    1xFLAG

    Expression

    Mammalian

    Available Since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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32. pHR-SFFV-GFP1-10

    Plasmid

    #80409

    Purpose

    Expresses GFP1-10 in mammalian cells

    Depositor

    Bo Huang

    Article

    Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

    Insert

    GFP1-10
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Dec. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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33. pLEX305_3xFLAG_STAT1

    Plasmid

    #220323

    Purpose

    Expresses full length human STAT1 with an N-terminal 3xFLAG tag

    Depositor

    Jonathan Brown

    Insert

    STAT1 alpha (STAT1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Promoter

    PGK

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Braf

    Plasmid

    #40775

    Depositor

    Dustin Maly

    Article

    Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

    Insert

    Braf (BRAF Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Starting Met removed

    Promoter

    CMV

    Available Since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRDA_479

    Plasmid

    #179099

    Purpose

    BE Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    ABE8e (SpG)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRK5 beta4

    Plasmid

    #16037

    Depositor

    Filippo Giancotti

    Article

    Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

    Insert

    beta4 (ITGB4 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLX304-KDM6A

    Plasmid

    #203580

    Purpose

    Expresses wild-type KDM6A in mammalian cells.

    Depositor

    Abhishek Chakraborty, William Kaelin

    Article

    Chakraborty et al Science. 2019 Mar 15;363(6432):1217-1222

    Insert

    KDM6A (KDM6A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRK5_mCherry_P2AT2A_GFP-nb-2xKS

    Plasmid

    #238236

    Purpose

    For overexpression of mCherry_P2AT2A_GFP-nb-2xKS

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry_P2AT2A_GFP-nb-2xKS
    

    Expression

    Mammalian

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TFORF2554

    Plasmid

    #144129

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    AR (AR Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDONR223_CDKN1A_WT

    Plasmid

    #82201

    Purpose

    Gateway Donor vector containing CDKN1A , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    CDKN1A (CDKN1A Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge