We narrowed to 26,599 results for: vit;

1. pCMV-BE4-PpAPOBEC1 H122A

   Plasmid

   #138338

   Purpose

   Mammalian expression plasmid for BE4-PpABOBEC1H122A

   Depositor

   Nicole Gaudelli

   Article

   Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

   Insert

   BE4-PpAPOBEC1 H122A
   

   Use

   Synthetic Biology

   Tags

   BP-NLS

   Mutation

   Change of His122 to Ala.

   Promoter

   CMV

   Available Since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 V5 His-TOPO-GluCl optalpha-mYFP L9'F (Opt α-GluClv2.0)

   Plasmid

   #47387

   Purpose

   allow pharmacologically induced silencing of electrical activity in CNS neurons upon exposure to the anthelmintic drug ivermectin

   Depositor

   Henry Lester

   Article

   Frazier et al J Biol Chem. 2013 May 29.

   Insert

   glutamate-gated chloride Channels
   

   Expression

   Mammalian

   Mutation

   Leu9 prime mutated to F (Phenylalanine in M3-M4 l…

   Promoter

   CMV

   Available Since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-Basic-hCYP26A1P-E4-mCherry

   Plasmid

   #135478

   Purpose

   Human short form promoter of human gene in pGL3-Basic-mCherry plasmid vector (Namely, E4.2)

   Depositor

   Catharine Ross

   Article

   Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

   Insert

   mCherry Open reading Frame
   

   Use

   Unspecified

   Promoter

   Short from promoter of human CYP26A1 (312 bp)

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAFNF-ECFP-Actb

   Plasmid

   #169787

   Purpose

   An actin FRET sensor. Must be expressed with Venus-actin for FRET.

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   Actb (Actb Mouse)
   

   Tags

   ECFP

   Expression

   Mammalian

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRRL-5xTetO-pEF-H2B-Citrine-SV40

   Plasmid

   #186966

   Purpose

   Lentiviral vector encoding fluorescent reporter (5xTetO-pEF-H2B-Citrine-SV40) for expression in mammalian cells.

   Depositor

   Brian Liau

   Article

   Lue et al Nat Chem Biol. 2023 Feb;19(2):176-186. d

   Insert

   5xTetO-pEF-H2B-Citrine-SV40
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   5xTetO-pEF-1alpha

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pILGFP95

   Plasmid

   #83550

   Purpose

   Used to evaluate the expression output of PDA1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   PDA1 promoter-yEGFP
   

   Expression

   Yeast

   Promoter

   PDA1

   Available Since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-Lyn-μDpTT

   Plasmid

   #182321

   Purpose

   Plasma membrane-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   Lyn-μDronpa-TagRFP-T
   

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Mutation

   Dronpa contains H193T mutation.

   Promoter

   CMV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-CYP450-μDpTT

   Plasmid

   #182325

   Purpose

   Endoplasmic reticulum-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   DAKAP-μDronpa-TagRFP-T
   

   Tags

   N-terminal 27 amino acids from CYP450

   Expression

   Mammalian

   Mutation

   Dronpa contains H193T mutation.

   Promoter

   CMV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Lifeact-μDpTT

   Plasmid

   #182322

   Purpose

   Actin-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.

   Depositor

   Jin Zhang

   Article

   Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951

   Insert

   Lifeact-μDronpa-TagRFP-T
   

   Tags

   N-terminal 17 amino acids from budding yeast Abp1…

   Expression

   Mammalian

   Mutation

   Dronpa contains H193T mutation.

   Promoter

   CMV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. SG3ΔENV CA E28A,E29A

    Plasmid

    #145785

    Purpose

    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and E28A,E29A mutation in capsid (CA E28A,E29A).

    Depositor

    Wesley Sundquist

    Article

    Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

    Insert

    Capsid
    

    Use

    Lentiviral and Retroviral

    Expression

    Mammalian

    Mutation

    Changed Capsid residues Glutamic Acid residues 28…

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFH6

    Plasmid

    #86555

    Purpose

    Subcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiency

    Depositor

    Andreas Weber

    Article

    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

    Insert

    U6-26p::sgRNA scaffold
    

    Use

    CRISPR; Subcloning

    Promoter

    Arabidopsis U6-26 promoter

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFLAG-ING4

    Plasmid

    #13291

    Depositor

    Curtis Harris

    Article

    Shiseki et al Cancer Res. 2003 May 15. 63(10):2373-8.

    Insert

    ING4 (ING4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pLKO.1-sh-mSlug-3

    Plasmid

    #40647

    Depositor

    Bob Weinberg

    Article

    Guo et al Cell. 2012 Mar 2;148(5):1015-28.

    Insert

    sh-mSlug-3 (Snai2 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pLEG(R1-R4) #BGV057

    Plasmid

    #48957

    Purpose

    Lentiviral Destination vector containing AttR1-AttR4 site. Use with 4-way LR reaction with cDNA in entry vector, marker in pBEG R2-L3 plasmids and shRNA in pBEG R3-L4 plasmid (module 2,3,and 4)

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

15. hLOXL2-S300P

    Plasmid

    #200061

    Purpose

    LOXL2 mutant

    Depositor

    Lakshmi Santhanam

    Article

    Wang et al Commun Biol. 2023 Apr 7;6(1):375. doi: 1

    Insert

    human lysyl oxidase like 2 (LOXL2 Human)
    

    Expression

    Mammalian

    Mutation

    S300P mutation

    Available Since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-FRB:Akt2

    Plasmid

    #72899

    Purpose

    Recruitable Akt2 (LocaTOR2)

    Depositor

    Ivan Yudushkin

    Article

    Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

    Insert

    Serine/threonine protein kinase Bβ (AKT2 Human)
    

    Use

    Lentiviral

    Tags

    FRB(T2098L)

    Expression

    Mammalian

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBEG R3-miRNA(ccdB)-L4 #BGV228

    Plasmid

    #48953

    Purpose

    To create an AttR3-AttL4 flanked Entry vector encoding a miR30-embedded shRNA to knockdown targeted gene expression. (This corresponds to a module 4 plasmid).

    Depositor

    David Dankort

    Article

    Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

    Type

    Empty backbone

    Use

    Gateway cloning vector

    Promoter

    na

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pET28a-SENP5 (catalytic domain)

    Plasmid

    #16358

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP5 catalytic domain (SENP5 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 567 to 755 catalytic domain of human S…

    Available Since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pCDH-CIBN-CAAX

    Plasmid

    #188989

    Purpose

    A lentiviral plasmid encoding plasma membrane-tagged CIBN

    Depositor

    Jeremy Baskin

    Article

    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

    Insert

    CIBN (CIB1 Mustard Weed)
    

    Tags

    CAAX

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-cofilin S3A

    Plasmid

    #163604

    Purpose

    Expresses constitutively active cofilin in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    cofilin1 (Cfl1 Mouse)
    

    Expression

    Mammalian

    Mutation

    S3A substitution to make cofilin constitutively a…

    Available Since

    Aug. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV-hSynapsin1-axon-GCaMP6m

    Plasmid

    #111261

    Purpose

    For enriched expression of GCaMP6m in axons

    Depositor

    Lin Tian

    Article

    Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

    Has Service

    AAV9

    Insert

    axon-GCaMP6m
    

    Use

    AAV

    Tags

    GAP43 palmitoylation domain

    Expression

    Mammalian

    Promoter

    hSynapsin1

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. SG3ΔENV CA Q63A,Q67A

    Plasmid

    #145784

    Purpose

    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and Q63A,Q67A mutation in capsid (CA Q63A,Q67A).

    Depositor

    Wesley Sundquist

    Article

    Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

    Insert

    Capsid
    

    Use

    Lentiviral and Retroviral

    Expression

    Mammalian

    Mutation

    Changed Capsid residues Glutamines 63 and 67 to a…

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GFP-Myo 1d

    Plasmid

    #134906

    Purpose

    Expression of rat Myo 1d (Myr 4) in mammalian cells.

    Depositor

    Martin Bähler

    Article

    Bahler et al J Cell Biol. 1994 Jul;126(2):375-89. doi

    Insert

    Myo 1d (rat) with 3' UTR (Myo1d Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV IE

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pssAAV_Seq17_CMV_bglobin_3NLS_Venus_WPRE_polyA

    Plasmid

    #220224

    Purpose

    AAV-STARR-seq validation control vector with Venus

    Depositor

    Simon Rumpel

    Article

    Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-FLAG-SpCas9-HF1

    Plasmid

    #126770

    Purpose

    Expression of increased fidelity SpCas9-HF1 in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    T7

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pT7-AsCas12a_crRNA-site4 (MSP3511)

    Plasmid

    #160139

    Purpose

    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #4

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    AsCas12a crRNA with spacer #4 (spacer=GGAATCCCTTCTGCAGCACCTGG)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Promoter

    T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p944-SWITCH-OVER insert: EF1a-Puro

    Plasmid

    #217891

    Purpose

    insert vector for Switch-OVER: Single loxP-STOP-EF1a-Puro-mU6

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Flag Nprl2 pLJM1

    Plasmid

    #46338

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Nprl2 (NPRL2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

29. pFUGW-ZipACR-EYFP

    Plasmid

    #88844

    Purpose

    Fast anion channelrhodopsin (current half-decay time 2-4 ms depending on voltage) for time-resolved inhibition of neuronal spiking

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    ZipACR-NotI-EYFP
    

    Use

    Lentiviral

    Tags

    EYFP

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA-Stra6L-6His

    Plasmid

    #174215

    Purpose

    Stra6L-6His (N-Terminal) fusion protein driven by an CMV promotor. May be used to study the murine Vitamin A receptor Stra6L.

    Depositor

    Adrian Press

    Article

    Stafast et al ACS Appl. Polym. Mater. 2022

    Insert

    Stra6L (Stra6l Mouse)
    

    Tags

    6-His

    Expression

    Mammalian

    Mutation

    non

    Promoter

    CMV

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBabe-PI(WT)-Src(YF)-mCherry

    Plasmid

    #87357

    Purpose

    PI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutation

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    Src isoform 2 (Src Mouse)
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Y535F

    Promoter

    CMV

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. eNme2-C TadCBEa

    Plasmid

    #193840

    Purpose

    Express TadCBEa (with eNme2-C variant) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    eNme2-C TadCBEa
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTRCHisB-TRF2∆M

    Plasmid

    #50489

    Purpose

    E. Coli N-term HexHis Tagged Protein Expression Vector

    Depositor

    Jack Griffith

    Article

    Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

    Insert

    TRF2 Delta Myb (TERF2 Human)
    

    Tags

    6x His, T7 tag, and TEV protease site

    Expression

    Bacterial

    Mutation

    Contains amino acids 3-437

    Promoter

    pTrc

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCDNAIIIB cdc42 WT

    Plasmid

    #61635

    Purpose

    mammalian expression of cdc42 wt

    Depositor

    JS Gutkind

    Article

    Coso et al Cell. 1995 Jun 30;81(7):1137-46.

    Insert

    cdc42 (CDC42 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

35. p2CT-His-MBP-Lbu_C2c2_R1079A

    Plasmid

    #83494

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (R1079A- pre-crRNA processing mutant)

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R1079A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R1079A

    Promoter

    T7

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV-BE4-RrA3F F130L

    Plasmid

    #138341

    Purpose

    Mammalian expression plasmid for BE4-RrA3F F130L

    Depositor

    Nicole Gaudelli

    Article

    Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

    Insert

    BE4-RrA3F F130L
    

    Use

    Synthetic Biology

    Tags

    BP-NLS

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCW-CCND1-HA-puro

    Plasmid

    #212670

    Purpose

    Express tagged cyclin D1

    Depositor

    Yumi Konagaya, Tobias Meyer

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    TRE

    Available Since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCAG-H2B-mRuby3-IRES-eGtACR1-ST

    Plasmid

    #108960

    Purpose

    Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    eGtACR1-ST
    

    Expression

    Mammalian

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFLAG-ING5

    Plasmid

    #13292

    Depositor

    Curtis Harris

    Article

    Shiseki et al Cancer Res. 2003 May 15. 63(10):2373-8.

    Insert

    ING5 (ING5 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

40. SNF-5 pXOOM

    Plasmid

    #48697

    Purpose

    Expressed SNF-5 in Xenopus oocytes or mammalian cells

    Depositor

    Dmitri Boudko

    Article

    Metzler et al J Exp Biol. 2013 Aug 1;216(Pt 15):2843-5

    Insert

    snf-5 (snf-5 Nematode)
    

    Use

    Xenopus oocyte expression

    Expression

    Mammalian

    Mutation

    K42E (when compared to NP_496735.1)

    Promoter

    T7

    Available Since

    Dec. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge