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Showing: 361 - 380 of 67199 results

  3. pcDNA-dCas9-FLp300

    Plasmid
    #61356
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Human, Synthetic, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available since
    April 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    61356-plasmid-map-sequence-id-498727

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  6. pCX-OKS-2A

    Plasmid
    #19771
    Purpose
    Non-integrating (episomal) expression of mouse Oct3/4, Klf4 and Sox2
    Depositor
    Shinya Yamanaka
    Publication
    Okita et al Science. 2008 Nov 7. 322(5903):949-53.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Oct3/4-2A-Klf4-2A-Sox2 (Sox2, Klf4, Pou5f1 Mouse)
    Expression
    Mammalian
    Mutation
    Three genes are connected with the foot-and-mouth…
    Available since
    Jan. 5, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    19771-plasmid-map-sequence-id-354143

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  8. pCMV-GCaMP5G

    Plasmid
    #31788
    Purpose
    a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.
    Depositor
    Douglas Kim, Loren Looger, GENIE Project
    Publication
    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GCaMP5G
    Tags
    6xHis, T7 epitope, and Xpress tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 22, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    31788-plasmid-map-sequence-id-228108

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  9. pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (AAV9)

    Viral prep
    #100854-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (#100854). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven GECO1a calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Douglas Kim, GENIE Project
    Publication
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    Syn
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  10. Inducible Caspex expression

    Plasmid
    #97421
    Purpose
    For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics
    Depositor
    Steven Carr, Samuel Myers
    Publication
    Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Caspex
    T2A-Gfp
    Use
    CRISPR
    Tags
    3xFLAG and V5
    Expression
    Mammalian
    Mutation
    dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …
    Promoter
    TRE3G
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    97421-plasmid-map-sequence-id-466895

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  14. lenti sgRNA(MS2)_puro backbone

    Plasmid
    #73795
    Purpose
    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Publication
    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73795-plasmid-map-sequence-id-181202

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Showing: 361 - 380 of 67199 results