167,825 results

1. DP6

   Plasmid

   #140446

   Purpose

   Untargetted bacterial drift

   Depositor

   David Liu

   Article

   Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

   Insert

   dnaQ926 dam seqA emrR ugi CDA1 gIII
   

   Expression

   Bacterial

   Available Since

   April 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB_iASCL1_P2A_DLX2_T2A_mCherry_Puro

   Plasmid

   #168806

   Purpose

   PiggyBac transposon construct with doxycycline inducible ASCL1, DLX2 + mCherry expression

   Depositor

   Prashant Mali

   Article

   Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

   Insert

   ASCL1-P2A-DLX2-T2A-mCherry
   

   Use

   Synthetic Biology; Piggybac

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. EB3-GFP

   Plasmid

   #190164

   Purpose

   Expresses EB3-GFP in mammalian cells

   Depositor

   Anna Akhmanova

   Article

   Stepanova et al J Neurosci. 2003 Apr 1;23(7):2655-64.

   Insert

   EB3-GFP
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGH8 - pRAB-3::mCherry::unc-54utr

   Plasmid

   #19359

   Depositor

   Erik Jorgensen

   Article

   Christian et al

   Insert

   Prab-3::mCherry
   

   Tags

   mCherry

   Expression

   Worm

   Available Since

   Oct. 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCYC1m_yeGFP

   Plasmid

   #64389

   Purpose

   encodes yeast enhanced GFP under control of minimal pCYC1 promoter

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   yeast enhanced GFP
   

   Expression

   Yeast

   Promoter

   pCYC1

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adrb1

   Plasmid

   #184294

   Purpose

   A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adrb1

   Depositor

   Bernardo Sabatini

   Insert

   sgRNA-Adrb1
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-Tox-IRES-eGFP

   Plasmid

   #165427

   Purpose

   Expresses TOX in mammalian cells

   Depositor

   Anjana Rao, Patrick Hogan

   Article

   Seo et al Proc Natl Acad Sci U S A. 2019 Jun 18;11

   Insert

   TOX (Tox Mouse)
   

   Tags

   3xflag 5' of Tox

   Expression

   Mammalian

   Promoter

   5' LTR

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCFJ2060 - [1-2] ENTR - Tag - Snap (intron(1), syntrons(3), no_atg, no_stop)

   Plasmid

   #159873

   Purpose

   Three-fragment Gateway compatible protein tag for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Tag - Snap (intron(1), syntrons(3), no_atg, no_stop)
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ203 (pMDC32-Ubi1)

   Plasmid

   #86207

   Purpose

   Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromiycin resistance marker

   Depositor

   Yiping Qi

   Article

   Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

   Type

   Empty backbone

   Use

   Gateway compatible attr1-attr2 destination vector

   Expression

   Plant

   Promoter

   Maize ubiquitin 1 (ZmUbi1)

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV-VPR-S-L1-dCjCas9 MiniCAFE

    Plasmid

    #169910

    Purpose

    Expression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Insert

    MiniCAFE
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D8A, HNH-truncation (Δ495–609 aa) in CjCas9

    Promoter

    CMV

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCLX-PGK-hTERT

    Plasmid

    #114315

    Purpose

    Constitutive expression of the hTert protein

    Depositor

    Patrick Salmon

    Insert

    hTERT (TERT Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. MCP-mCherry

    Plasmid

    #207668

    Purpose

    To express fluorescently labeled MS2 coat binding protein (MCP)

    Depositor

    Christine Mayr

    Article

    Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

    Insert

    MCP-mCherry
    

    Expression

    Mammalian

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Flag-HA-USP16

    Plasmid

    #22595

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP16 (USP16 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Dec. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. Ubc.Luc.IRES.Puro

    Plasmid

    #33307

    Depositor

    Linzhao Cheng

    Article

    Jang et al Mol Imaging. 2011 Apr;10(2):111-22.

    Insert

    Firefly Luciferase
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    Ubc

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFUSEss-CHIg-mG3_M18

    Plasmid

    #82356

    Purpose

    Expression plasmid coding for heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

    Insert

    mouse immunoglobulin heavy chain IgG3 isotype (Ighg3 Mouse)
    

    Tags

    none

    Expression

    Mammalian

    Mutation

    none

    Promoter

    hEF1-HTLV prom

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. htrpC6-YFP

    Plasmid

    #21084

    Depositor

    Craig Montell

    Article

    Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

    Insert

    TrpC6 (TRPC6 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. p2lox-cAMPr

    Plasmid

    #99143

    Purpose

    Expresses cAMPr in mammalian cells

    Depositor

    Justin Blau

    Article

    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

    Insert

    cAMPr
    

    Use

    Cre/Lox

    Tags

    cpGFP

    Expression

    Mammalian

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. ORFtag_TetR-SD_Frame2

    Plasmid

    #220982

    Purpose

    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame2

    Depositor

    Alexander Stark

    Insert

    TetR repressor
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. ORFtag_TetR-SD_Frame3

    Plasmid

    #220983

    Purpose

    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame3

    Depositor

    Alexander Stark

    Insert

    TetR repressor
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti PGK FKBP12(F36V)-OGT (Neo)

    Plasmid

    #154294

    Purpose

    Lentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin resistant) off PGK promoter

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    2x HA and FKBP12(F36V)

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLox-LV-pCMV-FLPo-pGK-mCl-GP33/66

    Plasmid

    #216482

    Purpose

    Expresses a lox-p flanked FLPo and mClover3 fluorescent protein with LMCV GP33-43 and GP66-77 epitopes embedded in loop of mclover for initiating and removing immunogenic tumor cells in KPFrt mice

    Depositor

    Nikhil Joshi

    Article

    Cheung et al Cancer Cell (2025), doi: 10.1016/j.ccell

    Inserts

    FLPo Recombinase
    mClover3-GP33/66
    

    Use

    Lentiviral

    Promoter

    pCMV and pGK

    Available Since

    Oct. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. LEGO-pDHS-ITDE-AP1x6-DUSP5

    Plasmid

    #217419

    Purpose

    Luciferase expression vector with an oncogene-inducible enhancer, 6 AP-1 sites, the full length DUSP5 promoter, and a chromatin priming element. Alias: LEGO-DUSP5p-AP-1x6-ITDe

    Depositor

    Peter Cockerill

    Insert

    Luciferase, GFP
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    Human DUSP5 promoter

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. GFP-ING2(pHD)

    Plasmid

    #21589

    Depositor

    Junying Yuan

    Article

    Gozani et al Cell. 2003 Jul 11. 114(1):99-111.

    Insert

    GFP-ING2(pHD) (Ing2 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains Ing2 plant homeodomain (PHD) amino acids…

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

24. pMRX-IP-EGFP-mouse Plaat3 (WT)

    Plasmid

    #167987

    Purpose

    Expresses EGFP-Plaat3 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

    Insert

    Hrasls3 (Plaat3 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pTD-NTwinStrep_Km

    Plasmid

    #45938

    Purpose

    use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Tags

    6xHis and Twin-Strep

    Expression

    Bacterial

    Promoter

    Trc (lactose/IPTG inducible)

    Available Since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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26. pUC18T-mini-Tn7T-Tp-dsRedExpress

    Plasmid

    #65036

    Purpose

    TpR mini-Tn7 vector, mobilizable, for RFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    dsRedExpress
    

    Expression

    Bacterial

    Available Since

    Aug. 14, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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27. pHR'-NRSF-CITE-GFP

    Plasmid

    #21310

    Depositor

    Jay Nadeau

    Article

    Nadeau et al J Neurophysiol. 2002 Jul . 88(1):409-21.

    Insert

    neuron restrictive silencer factor (Rest Mouse)
    

    Use

    Lentiviral

    Tags

    GFP (bicistronic with CITE/IRES)

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Oct. 20, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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28. pEGPN-TMPRSS2

    Plasmid

    #187484

    Purpose

    Expression vector - TMPRSS2

    Depositor

    Lewis Cantley

    Article

    Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

    Insert

    TMPRSS2 (TMPRSS2 Human)
    

    Use

    Lentiviral

    Promoter

    EFS

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. enIscB-T5E

    Plasmid

    #205411

    Purpose

    Vector encoding human codon-optimized enhanced-activity OgeuIscB fused with T5E at C-terminal driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s

    Insert

    pCAG_Flag_SV40NLS_OgeuIscB*_XTEN_T5E_npNLS_pU6__RNA*_pCMV_mCherry
    

    Expression

    Mammalian

    Mutation

    E85R+H369R+S387R+S457R

    Promoter

    CAG, hU6, CMV

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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30. ADORA2A-Tango

    Plasmid

    #66210

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    ADORA2A (ADORA2A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

31. pAAV-CAG-FlpO (AAV Retrograde)

    Viral Prep

    #183412-AAVrg

    Purpose

    Ready-to-use AAV Retrograde particles produced from pAAV-CAG-FlpO (#183412). In addition to the viral particles, you will also receive purified pAAV-CAG-FlpO plasmid DNA. CAG-driven expression of the recombinase FlpO. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Janelia Viral Tools

    Promoter

    CAG

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

32. mCherry-Calreticulin-N-16

    Plasmid

    #55006

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Calreticulin (CALR Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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33. pAAV-CAG-dLight1.3b (AAV1)

    Viral Prep

    #125560-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-CAG-dLight1.3b (#125560). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.3b plasmid DNA. CAG-driven expression of dLight1.3b dopamine sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Promoter

    CAG

    Available Since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

34. pCMV-dCas13-M3M14nes

    Plasmid

    #155367

    Purpose

    Targeted m6A RNA methylation in mammalian cells

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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35. pCMV-EB3mCherry_cmv-LifeActGFP

    Plasmid

    #133927

    Purpose

    Live dual labeling of the actin network and polymerizing microtubules for dynamic analysis of the cytoskeleton

    Depositor

    Frank Bradke, Sebastián Dupraz

    Article

    Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

    Insert

    EB3-mCherry and Lifeact-GFP (MAPRE3 Human)
    

    Expression

    Mammalian

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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36. pORI28

    Plasmid

    #71595

    Purpose

    EC1000 containing pORI28. Used as the source of plasmid pORI28. With pTRK669, part of a system for chromosomal integration in gram positive bacteria.

    Depositor

    Jan Kok, Todd Klaenhammer

    Article

    Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

    Type

    Empty backbone

    Use

    Integration vector for bacteria

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLenti-TripleColour-StAR

    Plasmid

    #222695

    Purpose

    Competition assay

    Depositor

    Ulrich Elling

    Article

    Uijttewaal et al Nat Biotechnol. 2024 Dec 16. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP, miRFP703, dTomato

    Expression

    Mammalian

    Mutation

    PGK, TagBFP, dTomato without BsmBI cutsite

    Available Since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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38. pCAGGS-4EBP1 F113A

    Plasmid

    #81122

    Purpose

    expresses a constitutively active mutant of 4E-BP1 under the CAG promoter

    Depositor

    Angelique Bordey

    Article

    Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

    Insert

    4EBP1 F113A (Eif4ebp1 Mouse)
    

    Expression

    Mammalian

    Mutation

    F113A mutation

    Promoter

    CAG

    Available Since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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39. pmVenus(L68V)-mTurquoise2

    Plasmid

    #60493

    Purpose

    Produces a fusion between mTurquoise2 and the FRET acceptor mVenus(L68V). It can be used as a positive control for FRET from mTurquoise2 to mVenus(L68V)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    SYFP2-mTurquoise2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

    Plasmid

    #183409

    Purpose

    piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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