We narrowed to 22,109 results for: ESC

1. pSC101-GFPmut2-mScarlet-I TGA

   Plasmid

   #208189

   Purpose

   nonsense mistranslation, TGA stop

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Insertion 6TGA*(stop) 7AGT(Ser)

   Promoter

   Ptet+dnaK P1

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSC101-GFPmut2-mScarlet-I TGATC

   Plasmid

   #208190

   Purpose

   nonsense mistranslation, TGA stop variant 2

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Insertion 6TGA*(stop) 7TCT(Ser)

   Promoter

   Ptet+dnaK P1

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSC101-GFPmut2-mScarlet-I T7

   Plasmid

   #208191

   Purpose

   frameshift mistranslation, T7

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Frameshift insertion 6TTTTTTT

   Promoter

   Ptet+dnaK P1

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSC101-GFPmut2-mScarlet-I A5

   Plasmid

   #208192

   Purpose

   frameshift mistranslation, A5

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Frameshift insertion 6AAAAA

   Promoter

   Ptet+dnaK P1

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSC101-GFPmut2-mScarlet-I A4

   Plasmid

   #208193

   Purpose

   frameshift mistranslation, A4

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Frameshift insertion 6AAAA

   Promoter

   Ptet+dnaK P1

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSC101-GFPmut2-mScarlet-I A6T

   Plasmid

   #208194

   Purpose

   frameshift mistranslation, A6T

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Frameshift insertion 6AAAAAAT

   Promoter

   Ptet+dnaK P1

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSC101-GFPmut2-mScarlet-I C3T

   Plasmid

   #208195

   Purpose

   frameshift mistranslation, C3T

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Frameshift insertion 6CCCT

   Promoter

   Ptet+dnaK P1

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSC101-GFPmut2-mScarlet-I 6Trp7Ser

   Plasmid

   #208186

   Purpose

   mistranslation GFP and mScarlet-I positive control with Trp insertion

   Depositor

   Tanel Tenson

   Article

   Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

   Inserts

   GFPmut2
   mScarlet-I
   

   Use

   Reporter

   Tags

   Expression

   Bacterial

   Mutation

   Insertion 6Trp7Ser

   Promoter

   Ptet+dnaK P1

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMH062

   Plasmid

   #225537

   Purpose

   Expresses IS621 in insect cells

   Depositor

   Hiroshi Nishimasu

   Article

   Hiraizumi et al Nature. 2024 Jun;630(8018):994-1002. doi

   Insert

   IS621
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKDR-TetOn

    Plasmid

    #183577

    Purpose

    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated Tet-On promoter)

    Depositor

    Erik Dent

    Article

    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

    Insert

    Unattenuated
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-On

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAJM.715

    Plasmid

    #108516

    Purpose

    PTac-YFP Reporter. IPTG sensor for use in Marionette strain.

    Depositor

    Christopher Voigt

    Article

    Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

    Insert

    PTac-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mScarlet-I-UtrCH

    Plasmid

    #98823

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    UtrCH
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pNB768

    Plasmid

    #60139

    Purpose

    Green click beetle luciferase from Promega driven by MET17 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    CBG99
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    MET17pr

    Available since

    Nov. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSF11-wmiRFP713-T2A-HO1

    Plasmid

    #197246

    Purpose

    Expresses the protein of wmiRFP713-T2A-HO1 in neurons of C. elegans

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    wmiRFP713-T2A-HO1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAGD236

    Plasmid

    #219497

    Purpose

    pCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoter

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    sfGFP, Eco1 msr, Eco1 RT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-CAG-miRFP680

    Plasmid

    #197203

    Purpose

    Expresses the protein of miRFP680 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    miRFP680
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMDICI

    Plasmid

    #51652

    Purpose

    E coli genetic modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    CmR
    

    Use

    Genetic modification

    Tags

    Expression

    Mutation

    Promoter

    unknown

    Available since

    July 7, 2014

    Availability

    Academic Institutions and Nonprofits only

18. mNeonGreen-UtrCH

    Plasmid

    #98879

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    UtrCH
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    *(see below)

    Promoter

    CMV

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. U_(+36GFP)-BirA

    Plasmid

    #71753

    Purpose

    U+36GFP-BirA for expression in E.Coli

    Depositor

    David Liu

    Article

    Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

    Insert

    U_(pos36GFP)_BirA_6XHis_LPETG
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PEP109E

    Plasmid

    #97395

    Purpose

    binary construct of cytosolic mCherry with sfGFP11 tag for plant expression

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

    Insert

    mCherry-sfGFP11
    

    Use

    Tags

    cytosol

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGilesSHdI_psmyc_His6_triple red

    Plasmid

    #170047

    Purpose

    Mono-Responsive Reporter Gene Constructs for triple red

    Depositor

    Clifton Barry III

    Article

    Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

    Inserts

    mScarlet
    COmScarlet
    mRuby3
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFA6a-link-yomTagBFP-SpHis5

    Plasmid

    #44838

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mTagBFP with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomTagBFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKI1

    Plasmid

    #199118

    Purpose

    Expresses N-terminally Hisx6-tagged, SNAP-tagged full-length RapA in E. coli cells, suitable for fluorescent labelling

    Depositor

    Jeff Gelles

    Article

    Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12

    Insert

    H6-SNAP-RapA
    

    Use

    Tags

    6xHis and SNAP-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLV-EgI-ZeoR

    Plasmid

    #207178

    Purpose

    Gateway compatible Lentiviral Expression Plasmid EF1a-IRES-ZeoR

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAV-shRNA-Tet2

    Plasmid

    #85743

    Purpose

    shRNA against mouse Tet2

    Depositor

    Hongjun Song

    Article

    Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

    Insert

    shRNA Tet2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    Available since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. VAMP2-SNAP

    Plasmid

    #105288

    Purpose

    VAMP2 tagged with SNAP

    Depositor

    Justin Taraska

    Article

    Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

    Insert

    VAMP2-SNAP (Vamp2 Rat)
    

    Use

    Tags

    SNAP tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBABE-puro-NLS-LSSmKate2

    Plasmid

    #34586

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    NLS-LSS-mKate2
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

    Promoter

    Available since

    Feb. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBAD_IPF2.0

    Plasmid

    #163124

    Purpose

    A plasmid with IFP2.0 coding sequence coded into it. Expresses IFP2.0 with N-term His-tag.

    Depositor

    Julie Biteen

    Article

    Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

    Insert

    IFP2.0
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHPdesteGFP

    Plasmid

    #24566

    Depositor

    Ingrid Lohmann

    Article

    Boy et al Genesis. 2010 Apr 22. [Epub ahead of pri

    Insert

    
    

    Use

    Reporter construct

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pKDR-CBh

    Plasmid

    #183557

    Purpose

    One in a series of plasmids to titrate levels of expressed protein to levels of endogenous protein (non-attenuated CBh promoter)

    Depositor

    Erik Dent

    Article

    Millette et al Front Genome Ed. 2022 Mar 28;4:803375. d

    Insert

    Unattenuated
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    June 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Ii-Str_puromycin

    Plasmid

    #65309

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Invariant chain (p33)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Streptavidin in its lumenal part

    Promoter

    CMV

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA5/FRT/TO_CalR_HaloTag7_KDEL

    Plasmid

    #175530

    Purpose

    Expression of HaloTag7 in the endoplasmid reticulum of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    CalR-HaloTag7-KDEL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAG308-His-redFAST

    Plasmid

    #160350

    Purpose

    Expresses His-tagged redFAST in bacteria

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

    Insert

    His-redFAST
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMVMagFRET-1-NLS

    Plasmid

    #50748

    Purpose

    Expresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.

    Depositor

    Maarten Merkx

    Article

    Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

    Insert

    gene encoding for MagFRET-1 with NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pPAiRFP1-N1

    Plasmid

    #44272

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Nat Commun. 2013 Jul 10;4:2153. doi: 10.

    Insert

    PAiRFP1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G127D/S141R/M163L/Q168L/A203V/G218S/R220P/V244F/A…

    Promoter

    CMV

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAG261-His-greenFAST

    Plasmid

    #160349

    Purpose

    Expresses His-tagged greenFAST in bacteria

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

    Insert

    His-greenFAST
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRK5-SNAP-CLIP-GB1a

    Plasmid

    #125709

    Purpose

    Eukaryotic expression of a fusion protein encoding SNAP, CLIP and the GB1a subunit of the metabotropic GABA receptor

    Depositor

    Kai Johnsson

    Article

    Masharina et al J Am Chem Soc. 2012 Nov 21;134(46):19026

    Insert

    SNAP-CLIP-GB1a
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMito.iGABASnFR.F102Y.Y137L

    Plasmid

    #112177

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.F102Y.Y137L
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F102Y.Y137L

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHPdestmCherry

    Plasmid

    #24567

    Depositor

    Ingrid Lohmann

    Article

    Boy et al Genesis. 2010 Apr 22. [Epub ahead of pri

    Insert

    
    

    Use

    Reporter construct

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRF0G-Tyr

    Plasmid

    #113644

    Purpose

    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    MjYRS and tRNA gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge