We narrowed to 20,059 results for: ato
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Plasmid#65316Purposereceiving vector of terminators for the construction of standard terminator parts in YeastFab workDepositorInsertRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYZ_3WJrep_N10_trigger
Plasmid#132728PurposeT7 RNAP-driven expression of 3WJ repressor index 10 trigger RNADepositorInsert3WJ_N10_trigger
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYZ_3WJrep_N10_switch
Plasmid#132724PurposeT7 RNAP-driven expression of 3WJ repressor index 10 switch RNA with a GFP-ASV reporterDepositorInsert3WJ_N10_switch
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJH199_pE12_hyCal2-mCherry
Plasmid#160412PurposeGateway entry clone containing postsynaptic HyCal component (HyNaC2) for HySyn system fused to mCherryDepositorInsertpDONR221 HyCal2::mCherry
UseOtherTagsmCherryExpressionMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pALPS_3'LTR GFP@gag start SFFV rev
Plasmid#101339PurposeLTR drives GFP and internal SFFV promoter drives codon optimized revDepositorInsertGFP, rev
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAN-PBAD-sgRNA-scramble
Plasmid#62285Purposeexpression of scramble sgRNA from the arabinose-inducible promoterDepositorInsertscramble sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterpBADAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP Δgag/pol
Plasmid#101343PurposeDeletion from the start of gag until 229 bases before the cPPTDepositorInsertNL4-3
UseLentiviralTagsExpressionMutationdelta GagPolPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET5
Plasmid#107437PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorInsertMET5 (MET5 Budding Yeast)
UseTagsV5-HisExpressionYeastMutationV81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …PromoterGAL1Available sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_AKAR5Δ
Plasmid#119912PurposeMammalian expression of the AKAR5Δ sensor with N-terminal His tag for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInsertAKAR5Δ
UseTags6xHisExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKB237
Plasmid#170030PurposeEncodes LacI-controlled expression of surface-displayed CCL19 and PvirK-mNeonGreen reporterDepositorArticleInsertsUseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCLXSN(GFP)-myc-BioID-C
Plasmid#174737PurposeOverexpresionDepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A2 (2)
Plasmid#185852PurposeTesting the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
2674 pBS Bcl-2 P1+P2
Plasmid#8746DepositorInsertBcl-2 P1+P2 (BCL2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pHB4
Plasmid#53462PurposeThis plasmid contains an ADHpr driven chimeric protein with SNC1, N-terminal GFP and C-terminal SUC2. It contains flanking homology to SUC2, allowing optional integration.DepositorInsertyeGFP-Snc1-Suc2
UseTagsExpressionYeastMutationPromoterADHAvailable sinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-004
Plasmid#78691PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-003
Plasmid#78690PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
C514-E23: attB5r-RU5u-3xFLAG-nostop-attB1r
Plasmid#162958PurposeGateway attB5r/attB1r entry clone for generation of N-terminal 3xFLAG-tagged fusion proteinsDepositorInserttriple FLAG epitope tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
sspa-GCaMP6s
Plasmid#67559Purposeshort photoactivatable fluorescent calcium reporterDepositorInsertspa-GCaMP6s
UseTagsExpressionMammalianMutationK65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…PromoterAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXD70LacZ3-Pcsd-LacZ
Plasmid#191619PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), Pcsd promoter-lacZ, E. lenta constitutive promoterDepositorInsertlacZ
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT eGFP-RVFV NSs HhRz
Plasmid#91801PurposeExpresses a non-polyadenylated, translatable EGFP mRNA in DrosophilaDepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-013
Plasmid#78701PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
MTK1_027
Plasmid#123689PurposeEncodes ConL5 and human E4 insulator as a Type 1 part to be used in the MTK systemDepositorInsertConnector L5-E4 insulator
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK1_028
Plasmid#123690PurposeEncodes ConL6 and human F1 insulator as a Type 1 part to be used in the MTK systemDepositorInsertConnector L6-F1 insulator
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK1_029
Plasmid#123691PurposeEncodes ConL7 and human B2 insulator as a Type 1 part to be used in the MTK systemDepositorInsertConnector L7-B2 insulator
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ChrimsonR-LSSmOrange
Plasmid#149691PurposeFor packaging and expression of ChrimsonR-LSSmOrange in neurons via AAVDepositorInsertChrimsonR
UseAAVTagsLSSmOrangeExpressionMammalianMutationPromoterhSynAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM6-H9-mCherry
Plasmid#66532PurposeExpresses mCherry under the control of Mutant T7 Promoter H9DepositorInsertmCherry
UseSynthetic BiologyTagsExpressionBacterialMutationCodon Optimized for E. coliPromoterMutant T7 Promoter - H9Available sinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYZ_3WJrep_N11_trigger
Plasmid#132733PurposeT7 RNAP-driven expression of 3WJ repressor index 11 trigger RNADepositorInsert3WJ_N11_trigger
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
C234-E08: attB2r-3xFLAG-attB3
Plasmid#162912PurposeGateway attB2r/attB3 entry clone for construction of C-terminal triple-FLAG epitope tagged fusion proteinsDepositorInserttriple FLAG epitope tag
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E44: attB5r-SNAP-nostop-attB1r
Plasmid#162962PurposeGateway attB5r/attB1r entry clone for generation of N-terminal SNAP-tagged fusion proteinsDepositorInsertSNAP tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5_tAKARÎł
Plasmid#119915PurposeMammalian expression of the tAKARÎł sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInserttAKARÎł
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAG-EGFP-Otx2shRNA2
Plasmid#73982PurposeOtx2 shRNA2 (based on mir155) was inserted into the 3'UTR of EGFP.DepositorInsertEGFP-Otx2-shRNA2
UseRNAiTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET-triple mutation-pa-GCaMP6f
Plasmid#67570Purposebacterial expression of a photoactivatable fluorescent calcium reporterDepositorInsertpa-GCaMP6f
UseTagsHIS-tagExpressionBacterialMutationV115H, L64F, T65SPromoterT7Available sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
C514-E67: attB5r-RU5u-Strep2x2-nostop-attB1r
Plasmid#162967PurposeGateway attB5r/attB1r entry clone for generation of N-terminal dual Strep-tagged fusion proteinsDepositorInsertDual Strep2 purification tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry-CrxshRNA
Plasmid#73987PurposeCrx shRNA (mir30 backbone) was inserted into the 3'UTR of mCherry.DepositorInsertshRNA that targets the mouse Crx gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAct:MS2-p65-HSF
Plasmid#78902PurposeExpresses MS2-p65-HSF under actin promoter for SAM CRISPRa in Drosophila cellsDepositorInsertMS2-p65-HSF
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3.2mar-GFP
Plasmid#63694Purpose3.2 kb marine armor plate enhancer driving GFP reporterDepositorInsert3.2 kb marine stickleback armor plate enhancer
UseTagsEGFPExpressionMammalianMutationPromoterhsp70Available sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pILGFP9E3
Plasmid#185842PurposeTesting the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG-EGFP-Otx2shRNA1
Plasmid#73981PurposeOtx2 shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.DepositorInsertshRNA that targets the mouse Otx2 gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-MS2tail-SAM
Plasmid#78905PurposeVector for expressing a single sgRNA with SAM MS2 tail, under U6:3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceAug. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
Human NCKX2-AS-long (pcDNA3.1+)
Plasmid#75201PurposeMammalian expression of SLC24A2DepositorInsertSLC24A2 (SLC24A2 Human)
UseTagsExpressionMammalianMutationalternatively spliced exon presentPromoterCMVAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pALPS_3'LTR GFP@gag start SFFV tat
Plasmid#101338PurposeLTR drives GFP and internal SFFV promoter drives codon optimized tatDepositorInsertGFP, tat
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pOME0005R
Plasmid#178374PurposeExpresses KSHV ORF K2DepositorInsertKSHV ORF K2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOME0006L
Plasmid#178376PurposeExpresses KSHV K2 ORF taggedDepositorInsertKSHV ORF K2
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA FLAG TORC1 (1-630)
Plasmid#22974DepositorInsertTORC1 (1-630) (CRTC1 Human)
UseTagsFLAGExpressionMammalianMutationAmino acids 1-630 rather than 1-634.PromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-005
Plasmid#78692PurposeA weak promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPB-DEST-R1R2-pNeo
Plasmid#141057PurposeMutisite Gateway mediated vector constructionDepositorInsertNeomycin resistance gene
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-STOP-shA1
Plasmid#14422DepositorInsertmodified U6 promoter (U6lox), U6 STOP cassette, shA1 gene
UseCre/Lox and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pES002 (pTet-qacR wt)
Plasmid#69031Purposewild-type qacR downstream of a Tet promoterDepositorInsertqacR (qacR Synthetic)
UseTagsHis6 tagExpressionBacterialMutationPromoterpTetAvailable sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZF-BCB-GCN
Plasmid#127476Purposecoding sequence for synthetic zinc finger ZF-BCB-GCNDepositorInsertZF-BCB synthetic transcription factor fused with GCN4 leucine zipper
UseCell-free transcription–translationTagsGCN4 leucine zipperExpressionMutationPromoterT7Available sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only