167,825 results

1. pQE-60 roGFP2-Orp1-His

   Plasmid

   #64976

   Purpose

   Bacterial expression of His-tagged roGFP2-Orp1

   Depositor

   Tobias Dick

   Article

   Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Tags

   His and roGFP2

   Expression

   Bacterial

   Available Since

   June 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti-SpCas9 neo

   Plasmid

   #104996

   Purpose

   This lentiviral construct delivers hSpCas9 and G418 resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-CamKIIa-jGCaMP8m-WPRE (AAV Retrograde)

   Viral Prep

   #176751-AAVrg

   Purpose

   Ready-to-use AAV Retrograde particles produced from AAV-CamKIIa-jGCaMP8m-WPRE (#176751). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8m-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8m. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Loren Looger

   Promoter

   CamKIIalpha

   Available Since

   July 21, 2023

   Availability

   Academic Institutions and Nonprofits only

4. pLenti6.3/EV

   Plasmid

   #120848

   Purpose

   Control vector, 20nt random sequence between gateway recombination sites

   Depositor

   Jan Rehwinkel

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-32a(+)-NRG1B1

   Plasmid

   #120287

   Purpose

   Produce human recombinant NRG1 in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   NRG1-beta 1
   

   Tags

   6x His and S-Tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 promoter

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJR89

   Plasmid

   #140096

   Purpose

   sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.

   Depositor

   Jonathan Weissman

   Article

   Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

   Insert

   sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
   

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFH2.2_mCherry-MS2x12

   Plasmid

   #205521

   Purpose

   Express mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   mCherry
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJMP1356

   Plasmid

   #119276

   Purpose

   for cloning new sgRNAs

   Depositor

   Jason Peters, Oren Rosenberg, Carol Gross

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSEED

   Plasmid

   #219658

   Purpose

   Permits GoldenGate assembly of new SEED cassettes via Golden Gate assembly (BsaI)

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   Empty SEED Cassette (3xP3-dsRed)
   

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBK2044-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2

    Plasmid

    #223164

    Purpose

    2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCLX-PGK-hTERT

    Plasmid

    #114315

    Purpose

    Constitutive expression of the hTert protein

    Depositor

    Patrick Salmon

    Insert

    hTERT (TERT Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAG-dLight1.3b (AAV1)

    Viral Prep

    #125560-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-CAG-dLight1.3b (#125560). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.3b plasmid DNA. CAG-driven expression of dLight1.3b dopamine sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Promoter

    CAG

    Available Since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

13. pSNAPf-N1

    Plasmid

    #58187

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    SNAP tag

    Expression

    Mammalian

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCAP03-acc(3)IV

    Plasmid

    #69862

    Purpose

    pCAP03-acc(3)IV is a upgraded version of the pCAP01(Plasmid #59981)

    Depositor

    Bradley Moore

    Article

    Tang et al ACS Chem Biol. 2015 Oct 21.

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Nov. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEF1-Pls3-sfGFP

    Plasmid

    #205750

    Purpose

    Expresses sfGFP under IPTG inducible promoter Pls3 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Medium/High strength.

    Depositor

    Jerome Bonnet

    Article

    Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95

    Type

    Empty backbone

    Use

    Shuttle vector gram+ gram-

    Expression

    Bacterial

    Promoter

    Pls3 (Phyperspank mutant, IPTG inducible)

    Available Since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-HA-hM4D(Gi)-IRES-mCitrine

    Plasmid

    #50464

    Purpose

    Gi-coupled hM4D-IRES-mCitrine under the control of human synapsin promote

    Depositor

    Bryan Roth

    Insert

    hM4D(Gi)-IRES-mCitrine (CHRM4 Human)
    

    Use

    AAV

    Tags

    HA

    Promoter

    human Synapsin 1

    Available Since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-CMV-GFP

    Plasmid

    #67634

    Purpose

    AAV vector expressing GFP

    Depositor

    Connie Cepko

    Article

    Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

    Insert

    GFP
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLEX307-APN-blast

    Plasmid

    #158455

    Purpose

    Constitutive lentiviral expression of human APN.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    APN (ANPEP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. qTAG-N-Puro-mScarlet

    Plasmid

    #207689

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mScarlet cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #209730

    Purpose

    Expresses presynaptic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (synapto).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CamKII

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAKgfp1

    Plasmid

    #14076

    Depositor

    Attila Karsi

    Article

    Karsi et al Plasmid. 2007 May;57(3):286-95. doi: 10.

    Insert

    Green fluorescent protein
    

    Expression

    Bacterial

    Available Since

    March 12, 2007

    Availability

    Academic Institutions and Nonprofits only

22. pLVX-mNINJ1-GFP

    Plasmid

    #208775

    Purpose

    Inducibly expresses mouse NINJ1 tagged with GFP

    Depositor

    Petr Broz

    Article

    Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

    Insert

    mNINJ1 (Ninj1 Mouse)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. LentiGuide-Neo

    Plasmid

    #139449

    Purpose

    Lentiviral vector with gRNA scaffold and neomycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    no sgRNA inserted; resistance gene: neoR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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24. EB3-GFP

    Plasmid

    #190164

    Purpose

    Expresses EB3-GFP in mammalian cells

    Depositor

    Anna Akhmanova

    Article

    Stepanova et al J Neurosci. 2003 Apr 1;23(7):2655-64.

    Insert

    EB3-GFP
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pDONR223_STAT3_WT

    Plasmid

    #82235

    Purpose

    Gateway Donor vector containing STAT3, part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, David Root, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    STAT3 (STAT3 Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLG014 (RADAR)

    Plasmid

    #157892

    Purpose

    Expresses the RADAR defense system

    Depositor

    Feng Zhang

    Article

    Gao et al Science. 2020 Aug 28;369(6507):1077-1084

    Insert

    RADAR
    

    Expression

    Bacterial

    Available Since

    Jan. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pZR071_SFFV-dCas9-mCherry-KRAB

    Plasmid

    #180264

    Purpose

    Lentiviral expression vector for dCas9-KRAB in mammalian cells

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    KRAB and mCherry

    Expression

    Mammalian

    Mutation

    Nuclease dead Cas9

    Promoter

    SFFV

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCDNA3-HA-HA-humanCMYC

    Plasmid

    #74164

    Purpose

    Mammalian expression of human cMyc

    Depositor

    Martine Roussel

    Article

    Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

    Insert

    cMyc (MYC Human)
    

    Tags

    2x HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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29. TRE reporter backbone

    Plasmid

    #158678

    Purpose

    This plasmid serves as the backbone for the TRE reporters described in our paper. It's a promoterless-mStrawberry-pGK-BSD backbone to which we insert a pathway specific promoter before the mStrawberry

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    mStrawberry

    Expression

    Mammalian

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc)

    Plasmid

    #140659

    Purpose

    OsTIR1(F74G)-P2A-mAID-EGFP-Nluc

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    OsTIR1(F74G) mAID-EGFP-Nluc TOL2
    

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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31. pMX229

    Plasmid

    #23002

    Depositor

    Linda Wordeman

    Article

    Stumpff et al Dev Cell. 2008 Feb . 14(2):252-62.

    Insert

    Kif18A (KIF18A Human)
    

    Tags

    6His and EGFP

    Expression

    Mammalian

    Available Since

    Feb. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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32. GFP-PH-FAPP1

    Plasmid

    #161986

    Purpose

    Expresses GFP-tagged PH-FAPP1. PI(4)P biosensor

    Depositor

    Tamas Balla, David Bryant

    Article

    Balla et al Mol Biol Cell. 2005 Mar;16(3):1282-95. d

    Insert

    PH-FAPP1 (PLEKHA3 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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33. pET_RTX_(exo-)

    Plasmid

    #102786

    Purpose

    Expresses the Reverse Transcriptase gene (3'-5' exonuclease deficient mutant)

    Depositor

    Andrew Ellington

    Article

    Ellefson et al Science. 2016 Jun 24;352(6293):1590-3. d

    Insert

    reverse transcription xenopolymerase with 3'-5' deficient exonuclease
    

    Expression

    Bacterial

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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34. pCS2-Jmjd3-F

    Plasmid

    #17440

    Purpose

    Expression of Jmjd3 in mammalian cells

    Depositor

    Kai Ge

    Article

    Hong et al Proc Natl Acad Sci U S A. 2007 Nov 20. 1

    Insert

    JMJD3 (Kdm6b Mouse)
    

    Tags

    6xMyc and Flag

    Expression

    Mammalian

    Available Since

    Feb. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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35. PB_iASCL1_P2A_DLX2_T2A_mCherry_Puro

    Plasmid

    #168806

    Purpose

    PiggyBac transposon construct with doxycycline inducible ASCL1, DLX2 + mCherry expression

    Depositor

    Prashant Mali

    Article

    Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

    Insert

    ASCL1-P2A-DLX2-T2A-mCherry
    

    Use

    Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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36. pCMV-dCas13-inactive M3nls

    Plasmid

    #157854

    Purpose

    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-inactive dCas13-inactive M3nls (METTL3 Cas13b is from Prevotella sp. P5-125; METTL3 is from H. sapiens)
    

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A; METTL3 D395A

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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37. beta-catenin (S33A, S37A, T41A, S45A)-pcw107-V5

    Plasmid

    #64613

    Purpose

    when used to produce lentivirus, express physiological levels of insert

    Depositor

    David Sabatini, Kris Wood

    Article

    Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

    Insert

    Ctnnb1 (Ctnnb1 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    S33A, S37A, T41A, S45A

    Promoter

    PGK

    Available Since

    May 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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38. pRK5-myc-Cdc42-Q61L

    Plasmid

    #12974

    Depositor

    Gary Bokoch

    Insert

    Cdc42 constitutively active (CDC42 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Q61L Constitutively active

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. LRP6-pCS2

    Plasmid

    #27242

    Depositor

    Xi He

    Article

    Tamai et al Nature. 2000 Sep 28. 407(6803):530-5.

    Insert

    low density lipoprotein receptor-related protein 6 (LRP6 Human)
    

    Use

    Xenopus injection

    Expression

    Mammalian

    Available Since

    Feb. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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40. Flag-Gsdmd-NT

    Plasmid

    #80951

    Purpose

    Expresses Gsdmd-NT in mammalian cells

    Depositor

    Judy Lieberman

    Article

    Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

    Insert

    Gsdmd-NT (Gsdmd Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge