Skip to main content
Addgene
Showing: 7921 - 7960 of 161241 results

  3. pCrysβ:ECFP-LexOP:mCherry-KrasG12V

    Plasmid
    #89888
    Purpose
    Human KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.
    Depositor
    Tatjana Sauka-Spengler
    Article
    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d
    Insert
    mCherry-KRasG12V
    Use
    Unspecified
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    89888-plasmid-map-sequence-id-181093

    Enlarge

  4. TFORF1522

    Plasmid
    #143288
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NR2E3 (NR2E3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143288-plasmid-map-sequence-id-396141

    Enlarge

  11. pSCM001

    Plasmid
    #124605
    Purpose
    Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH
    Depositor
    David Summers
    Article
    Zarkan et al Sci Rep. 2019 Mar 7;9(1):3868. doi: 10.1
    Insert
    mCherry-pHluorin
    Use
    Tags
    mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…
    Expression
    Bacterial
    Mutation
    Promoter
    pDAB
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    124605-plasmid-map-sequence-id-242761

    Enlarge

  12. pBMN HA

    Plasmid
    #188645
    Purpose
    This is the Lazarou lab's standard retrovirus vector backbone for expression of HA-tag fusions onto the N-terminus of cloned genes
    Depositor
    Michael Lazarou
    Article
    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    188645-plasmid-map-sequence-id-370206

    Enlarge

  15. Fragment1 with TLS2

    Plasmid
    #196983
    Purpose
    Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.
    Depositor
    Friedrich Kragler
    Article
    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    Intermediate fragment for assembly of gRNA-TLS2 construct
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196983-plasmid-map-sequence-id-390457

    Enlarge

  20. SETDB1

    Plasmid
    #25240
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SETDB1, SETDB1_16 (SETDB1 Human)
    Use
    Tags
    6xHis-TEV and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-EGFP

    Plasmid
    #118156
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-EGFP under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-EGFP
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a core and U6
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    118156-plasmid-map-sequence-id-239644

    Enlarge

  23. pBS513 EF1alpha-cre

    Plasmid
    #11918
    Purpose
    Expresses Cre under the control of the elongation factor-1 alpha (EF1alpha) promoter; includes upstream Kozak modification to increase expression.
    Depositor
    Brian Sauer
    Article
    Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.
    Insert
    cre (cre bacteriophage P1)
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1alpha
    Available since
    March 16, 2007
    Availability
    Academic Institutions and Nonprofits only
    11918-plasmid-map-sequence-id-163454

    Enlarge

  25. pAAV-CaMKIIa-EGFP (AAV8)

    Viral Prep
    #50469-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-CaMKIIa-EGFP (#50469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-EGFP plasmid DNA. CamKIIa-driven EGFP expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    CaMKII
    Tags
    EGFP
    Available since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only

  27. pLpA-DN.JNK2

    Plasmid
    #51943
    Purpose
    Adenoviral vector for dominant negative JNK2 expression
    Depositor
    Jeffery Molkentin
    Article
    Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.
    Insert
    DN JNK2 (Mapk9 Mouse)
    Use
    Adenoviral
    Tags
    HA
    Expression
    Mutation
    dominant negative: the dual phosphorylation moti…
    Promoter
    CMV
    Available since
    May 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  32. TFORF1108

    Plasmid
    #143254
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    CREBBP (CREBBP Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143254-plasmid-map-sequence-id-396151

    Enlarge

  33. pAAV_BiPVe3_dTomato_nlsdTomato (AAV PHP.eB)

    Viral Prep
    #213940-PHPeB
    Purpose
    Ready-to-use AAV PHP.eB particles produced from pAAV_BiPVe3_dTomato_nlsdTomato (#213940). In addition to the viral particles, you will also receive purified pAAV_BiPVe3_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the PV+ basket cell-targeting enhancer E3. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Gordon Fishell
    Article
    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1
    Promoter
    Tags
    dTomato
    Available since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only

  38. pAAV-hSyn-hChR2(H134R)-EYFP (AAV2)

    Viral Prep
    #26973-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-hSyn-hChR2(H134R)-EYFP (#26973). In addition to the viral particles, you will also receive purified pAAV-hSyn-hChR2(H134R)-EYFP plasmid DNA. hSyn-driven, humanized channelrhodopsin H134R mutant fused to EYFP for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    Syn
    Tags
    EYFP
    Available since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 7921 - 7960 of 161241 results