We narrowed to 7,460 results for: alp;

1. pFastBac1-OSF-TRIM5alpha(chimpanzee)

   Plasmid

   #79032

   Purpose

   Baculoviral entry vector to produce Bac-to-bac baculoviruses

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   OSF-TRIM5alpha(chimpanzee)
   

   Use

   Tags

   OneSTrEP-FLAG

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGV7 (CK2alpha' K69M, CK2beta)

   Plasmid

   #27095

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Inserts

   CK2beta (CSNK2B Human)
   CK2alpha' K69M (CSNK2A2 Human)
   

   Use

   Tetracycline-regulated expression

   Tags

   HA tag on CK2alpha' and Myc on CK2beta

   Expression

   Mammalian

   Mutation

   K69M on CK2alpha'

   Promoter

   bidirectional tet-responsive promoter

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTub-alpha1-Lifeact-GFP

   Plasmid

   #175437

   Purpose

   F-actin structures labelling

   Depositor

   Sebastián Dupraz, Frank Bradke

   Article

   Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

   Insert

   Lifeact-EGFP
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   pTub-alpha1

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSCALPS-Flag-Mili-EGFP

   Plasmid

   #212576

   Purpose

   Lentiviral expression of Flag-tagged mouse Mili in mammalian cells

   Depositor

   Phillip Zamore

   Article

   Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

   Insert

   Mili (Piwil2 Mouse)
   

   Use

   Lentiviral

   Tags

   5x Flag and SNAP tag

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ERalpha (Y537S mutant)-pcw107-V5

   Plasmid

   #64634

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   ESR1 (transcript variant 1) (ESR1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Y537S

   Promoter

   PGK

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(+)

   Plasmid

   #184334

   Purpose

   Expression of mouse Nrx3-alpha lacking insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

   Depositor

   Peter Scheiffele

   Article

   Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

   Insert

   Nrxn3-alpha (Nrxn3 Mouse)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPHR-mCherry-mHP1alpha-I163A

   Plasmid

   #181889

   Purpose

   Soluble light-dependent effector protein (HP1alpha with I163A mutation) for optodroplet formation or recruitment to CIBN localizers,

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   PHR-mCherry-mHP1alpha-I163A (Cbx5 Mouse, Mustard Weed)
   

   Use

   Tags

   PHR-mCherry

   Expression

   Mammalian

   Mutation

   I163A

   Promoter

   CMV

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPHR-mCherry-FUSN-mHP1alpha

   Plasmid

   #181892

   Purpose

   Soluble light-dependent effector protein (HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN) ) for optodroplet formation or recruitment to CIBN localizers

   Depositor

   Karsten Rippe

   Article

   Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

   Insert

   PHR-mCherry-FUSN-mHP1alpha (Cbx5 Mouse, Mustard Weed)
   

   Use

   Tags

   PHR-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CK2alpha V66A/I174A-HA (pJD23)

   Plasmid

   #37362

   Depositor

   David Litchfield

   Article

   Duncan et al Mol Cell Proteomics. 2008 Jun;7(6):1077-

   Insert

   CK2α V66A/I174A (CSNK2A1 Human)
   

   Use

   Tags

   HA X3

   Expression

   Mammalian

   Mutation

   V66A, I174A

   Promoter

   CMV

   Available since

   July 17, 2012

   Availability

   Academic Institutions and Nonprofits only

10. Integrin alpha5(1-623)-AHCys

    Plasmid

    #27301

    Depositor

    Timothy Springer

    Article

    Takagi et al EMBO J. 2003 Sep 15. 22(18):4607-15.

    Insert

    integrin alpha 5 (ITGA5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    alpha 5(1-623)+GG+TEV+GGEN+AHCys

    Promoter

    Available since

    March 8, 2011

    Availability

    Academic Institutions and Nonprofits only

11. isoform PKC Alpha (JCE599)

    Plasmid

    #89779

    Purpose

    yeast expression of mouse PKC Alpha with yeast PKC1 promoter and ADH1 terminator

    Depositor

    Juan Carlos Igual

    Article

    Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

    Insert

    PKC Alpha (Prkca Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    yeast PKC1 promoter (-600 to -1)

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pALPS puro miR30-STAT1

    Plasmid

    #117160

    Purpose

    Target site ATATCCAGTTCCTTTAGGGCC

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    shRNA targeting miR30
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HA-CK2alpha’ V67A/I175A (pJD24)

    Plasmid

    #37363

    Depositor

    David Litchfield

    Article

    Duncan et al Mol Cell Proteomics. 2008 Jun;7(6):1077-

    Insert

    CK2α' V67A/I175A (CSNK2A2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    V67A, I175A

    Promoter

    CMV

    Available since

    July 17, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pecALPS gag-gfp Blasti

    Plasmid

    #115805

    Purpose

    Express gag-gfp fusion driven by EIF1a promoter

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    gag-gfp
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EIF1a

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX 4t1 RARalpha (1-187)

    Plasmid

    #35558

    Depositor

    Jonathan Kurie, Harish Srinivas

    Article

    Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

    Insert

    RARalpha (RARA Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 1-187

    Promoter

    Available since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-alphaCaMKII-E2-Crimson-3XNLS-pA

    Plasmid

    #183803

    Purpose

    AAV vector to express nuclear localized E2-Crimson from CaMKIIa promoter

    Depositor

    Jonathan Ploski

    Article

    de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

    Insert

    E2-Crimson-NLS
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LentiCRISPR v2-sgAMPK alpha1 clone2

    Plasmid

    #162120

    Purpose

    Lentiviral sgRNA plasmid targeting human AMPK alpha1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgAMPK alpha1 (PRKAA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5-FRT-TO-hPKAalpha-Flag

    Plasmid

    #185380

    Purpose

    For mammalian expression of human PKAalpha-FLAG

    Depositor

    Ayaz Najafov

    Insert

    PRKACA (PRKACA Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-SYN-integrin alpha9-V5

    Plasmid

    #203729

    Purpose

    AAV vector plasmid expressing human integrin subunit alpha 9 (ITGA9) fused to V5-tag under the human synapsin (SYN) promoter

    Depositor

    Bart Nieuwenhuis, Richard Eva

    Insert

    ITGA9 (ITGA9 Human)
    

    Use

    AAV

    Tags

    V5-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Human synapsin promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pscALPS-SIV-RCM vpx

    Plasmid

    #115812

    Purpose

    Encodes codon optimized 3xFLAG-SIVRCM Vpx (AF349680) and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVRCM Vpx (AF349680)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Gal4 PGC1 alpha 1-350

    Plasmid

    #8893

    Depositor

    Bruce Spiegelman

    Article

    Puigserver et al Science 1999 Nov 12;286(5443):1368-71.

    Insert

    PGC-1 alpha (Ppargc1a Mouse)
    

    Use

    Tags

    Gal4 DBD

    Expression

    Mammalian

    Mutation

    amino acids 1-350 of PGC-1 alpha

    Promoter

    Available since

    Aug. 19, 2005

    Availability

    Academic Institutions and Nonprofits only

22. XE101 alpha transducin CS2+

    Plasmid

    #16681

    Depositor

    Randall Moon

    Article

    Slusarski et al Nature. 1997 Nov 27;390(6658):410-3. doi

    Insert

    alpha-transducin (GNAT1 Human)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

23. pEGB3 alpha1 Pnos:PhiC31int:Tnos (GB1531)

    Plasmid

    #160616

    Purpose

    TU for the constitutive expression of Streptomyces phage PhiC31 integrase.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    PhiC31
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ptcALPS gag-gfp Blasti

    Plasmid

    #115806

    Purpose

    Express gag-gfp fusion driven by TK promoter

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    gag-gfp
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TK

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pScalps eSpCas9_Zsgreen gRNA scaffold

    Plasmid

    #188449

    Purpose

    Expression of eSpCas9 tagged with Zsgreen

    Depositor

    Ajit Chande

    Article

    Mishra et al Mol Ther Nucleic Acids. 2022 Mar 8;28:20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1-alpha

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBSSK p85 alpha (delta BH)

    Plasmid

    #13431

    Depositor

    Ronald Kahn

    Article

    Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

    Insert

    p85 alpha (delta BH) (PIK3R1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    BH domain replaced by flag tag.

    Promoter

    Available since

    Nov. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

27. pTub-alpha-EB3-NeonGreen

    Plasmid

    #175263

    Purpose

    labelling of microtubule plus end and F-actin structures

    Depositor

    Sebastián Dupraz, Frank Bradke

    Article

    Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

    Insert

    EB3 (MAPRE3 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    pTub-alpha1

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTub-alpha1-iLifeact-NeonGreen

    Plasmid

    #175258

    Purpose

    F-actin structures labelling

    Depositor

    Sebastián Dupraz, Frank Bradke

    Article

    Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

    Insert

    iLifeact
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    pTub-alpha1

    Available since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEGB2alpha2 35s:hCas9:tNos (GB0639)

    Plasmid

    #68222

    Purpose

    Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    hCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pNTPTP alpha (S180A/S204A)

    Plasmid

    #17700

    Depositor

    Xinmin Zheng

    Article

    Zheng et al J Biol Chem. 2002 Jun 14. 277(24):21922-

    Insert

    PTPalpha S180A S204A (PTPRA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S180A / S204A

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

31. XE242 Mouse CK2alpha CS2p+

    Plasmid

    #16730

    Depositor

    Randall Moon

    Article

    Waxman et al Development. 2004 Dec. 131(23):5909-21.

    Insert

    mouse Casein Kinase II alpha-pCS2P+ (Csnk2a1 Mouse)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

32. pAAV-EF1alpha-soCoChR-GFP

    Plasmid

    #107709

    Purpose

    A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. EF1alpha promoter

    Depositor

    Edward Boyden

    Article

    Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

    Insert

    soCoChR-GFP
    

    Use

    AAV

    Tags

    EGFP and KA2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV5 Flag p38 alpha

    Plasmid

    #20786

    Depositor

    Roger Davis

    Article

    Raingeaud et al J Biol Chem. 1995 Mar 31. 270(13):7420-6

    Insert

    pCMV5 Flag p38 alpha (Mapk14 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

34. pFastBac1-OSF-TRIM5alpha(rhesus)

    Plasmid

    #79033

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(rhesus)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pscALPS-SIV-WRC-vpr

    Plasmid

    #115839

    Purpose

    Encodes codon optimized 3xFLAG-SIVWRC Vpr (AM713177) and puromycin resistance protein

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVWRC Vpr (AM713177)
    

    Use

    Lentiviral

    Tags

    3x-FLAG

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pNL-EGFP/EF1alpha/WPREdU3

    Plasmid

    #18698

    Depositor

    Jakob Reiser

    Article

    Ricks et al Stem Cells Dev. 2008 Jun . 17(3):441-50.

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCRII-TOPO GFR alpha 1

    Plasmid

    #119898

    Purpose

    Mouse GFR alpha 1 In situ hybridization probe

    Depositor

    Wenqin Luo, David Ginty

    Article

    Luo et al Neuron. 2009 Dec 24;64(6):841-56. doi: 1

    Insert

    GDNF family receptor alpha-1 precursor (Gfra1 Mouse)
    

    Use

    In situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pPD1025 EF1alpha TetON3G in TU6

    Plasmid

    #139262

    Purpose

    Expresses TetON3G under hEF1alpha in TUPV6

    Depositor

    Joshua Leonard

    Insert

    TetON3G
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1alpha

    Available since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. LV_EF1a_ERalpha(Y537S)-P2A-Hygro_Barcode

    Plasmid

    #170225

    Purpose

    Barcoded lentiviral vector to express ERalpha (Y537S) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    ER (ESR1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Y537S

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGEM-rat FceRI alpha

    Plasmid

    #8373

    Depositor

    Jean Kinet

    Article

    Kinet et al Biochemistry 1987 Jul 28;26(15):4605-10.

    Insert

    rat Fc epsilon RI alpha cDNA (Fcer1a Rat)
    

    Use

    Beta-gal

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2005

    Availability

    Academic Institutions and Nonprofits only