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Showing: 951 - 1000 of 1805 results

  17. LADL Bridge + Target Zfp462-Klf4SE

    Plasmid
    #127668
    Purpose
    Encodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promoters
    Depositor
    Jennifer Phillips-Cremins
    Article
    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s
    Insert
    CRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and hU6
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    127668-plasmid-map-sequence-id-247046

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  20. pMta2-EosHalo-TAFiP

    Plasmid
    #140959
    Purpose
    Targeting construct to make Mta2-Eos-Halo-Tev-Avi-3xFlag fusion
    Depositor
    Brian Hendrich
    Insert
    metastasis-associated gene family, member 2 (Mta2 Synthetic, Mouse)
    Use
    Mouse Targeting
    Tags
    3xFLAG, Avi, Eos, Halo, and TEV
    Expression
    Mutation
    Promoter
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    140959-plasmid-map-sequence-id-338415

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  24. pCDNA3-SREBP-1 S1P and S2P mutations (U124AFG200-5)

    Plasmid
    #210767
    Purpose
    Mammalian expression of SREBP-1 (isoform 1) with mutations in residues reported to be critical for S1P and S2P cleavage.
    Depositor
    Allan Weissman
    Article
    Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.
    Insert
    SREBP-1 (SREBF1 Human)
    Use
    Tags
    E-tag and T7x2
    Expression
    Mammalian
    Mutation
    DRSR 514-517 to AS; 557 R to A; multiple silent m…
    Promoter
    Available since
    April 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    210767-plasmid-map-sequence-id-421642

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  26. pcDNA3.1+FLAG-KrascomG12D

    Plasmid
    #206844
    Purpose
    To express mouse Kras encoded by common mouse codons (to increase expression) and with a G12D mutation with an N-terminal FLAG tag.
    Depositor
    Christopher Counter
    Article
    Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755
    Insert
    Kras (Kras Mouse)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Codons altered to most optimum based on mouse cod…
    Promoter
    Available since
    Oct. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    206844-plasmid-map-sequence-id-409574

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  27. cmamxA2-GFP

    Plasmid
    #107197
    Purpose
    Expresses human annexin A2-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deleted
    Depositor
    Volker Gerke, Ursula Rescher
    Article
    Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8
    Insert
    cmannexin A2 (ANXA2 Human)
    Use
    Tags
    Green Fluorescent Protein
    Expression
    Mammalian
    Mutation
    all type II Ca2+ binding sites deleted ( D161A, E…
    Promoter
    CMV
    Available since
    June 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    107197-plasmid-map-sequence-id-209669

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  28. pcDNA3.1+FLAG-KrascomQ61R

    Plasmid
    #206845
    Purpose
    To express mouse Kras encoded by common mouse codons (to increase expression) and with a Q61R mutation with an N-terminal FLAG tag.
    Depositor
    Christopher Counter
    Article
    Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755
    Insert
    Kras (Kras Mouse)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Codons altered to most optimum based on mouse cod…
    Promoter
    Available since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    206845-plasmid-map-sequence-id-441039

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  37. pAC-94N

    Plasmid
    #53281
    Purpose
    Contains crtI, crtY, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and produces beta-carotene in E. coli when complemented with a gene encoding geranylgeranyl diphosphate synthase
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E
    Insert
    crtY, crtI, crtB
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    endogenous promoters
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53281-plasmid-map-sequence-id-82992

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  44. pAC-85b

    Plasmid
    #53282
    Purpose
    Contains crtE, crtI, crtY genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and will produce beta-carotene in E. coli when complemented with a gene encoding phytoene synthase
    Depositor
    Francis X Cunningham Jr
    Article
    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E
    Insert
    crtE, crtY, crtI
    Use
    Low copy number bacterial cloning vector
    Tags
    Expression
    Mutation
    Promoter
    endogenous promoters
    Available since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53282-plasmid-map-sequence-id-82995

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  49. pMta2-EosHalo-TAFiP

    Plasmid
    #140959
    Purpose
    Targeting construct to make Mta2-Eos-Halo-Tev-Avi-3xFlag fusion
    Depositor
    Brian Hendrich
    Insert
    metastasis-associated gene family, member 2 (Mta2 Synthetic, Mouse)
    Use
    Mouse Targeting
    Tags
    3xFLAG, Avi, Eos, Halo, and TEV
    Expression
    Mutation
    Promoter
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    140959-plasmid-map-sequence-id-338415

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  50. pAAV2-CAGIG-WGA-mCherry

    Plasmid
    #224119
    Purpose
    To express the fusion protein composed of the Wheat germ agluttinin to the fluorescent protein mCherry. Labels the Golgi apparatus, ER and the secretory pathway. Secreted form, labels Lysosomes.
    Depositor
    Pantelis Tsoulfas
    Insert
    WGA-mCherry
    Use
    AAV
    Tags
    WGA and mCherry
    Expression
    Mammalian
    Mutation
    All mutations related to the WGA in this construc…
    Promoter
    CAGIG
    Available since
    Sept. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    224119-plasmid-map-sequence-id-446520

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Showing: 951 - 1000 of 1805 results