We narrowed to 15,993 results for: NSI

1. pAAV-anti-VEGF(Brolucizumab) PAGER(Gq)

   Plasmid

   #230014

   Purpose

   Expresses anti-VEGF PAGER(Gq) in mammalian cell

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   MT1-Brolucizumab-TEVcs-ALFA-hM1D
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   W442A on hM1D

   Promoter

   CMV

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV-anti-TNFalpha(Ozoralizumab) PAGER(Gq)

   Plasmid

   #230015

   Purpose

   Expresses anti-TNFalpha PAGER(Gq) in mammalian cells

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   MT1-Ozoralizumab-TEVcs-ALFA-hM1D
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   W442A on hM1D

   Promoter

   CMV

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-anti-mCherry(LaM6) PAGER(Gi)

   Plasmid

   #230012

   Purpose

   Expresses anti-mCherry PAGER(Gi) in mammalian cells

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   MT1-LaM6-TEVcs-ALFA-hM4D
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-anti-mCherry(LaM6) PAGER(G12)

   Plasmid

   #230013

   Purpose

   Expresses anti-mCherry PAGER(G12) in mammalian cells

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   MT1-LaM6-TEVcs-ALFA-hM1Dx12
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV-anti-GFP/BFP(LaG2) PAGER(FL)

   Plasmid

   #230022

   Purpose

   Expresses anti-GFP/BFP PAGER(FL) in mammalian cells

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   pAAV-MT3-LaG2-TEVcs-ALFA-hM4DxcpGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-anti-GFP(LaG16) PAGER(Gq)

   Plasmid

   #230006

   Purpose

   Expresses anti-GFP PAGER(Gq) in mammalian cells

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   MT1-LaG16-TEVcs-ALFA-hM1D
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   W442A on hM1D

   Promoter

   CMV

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-anti-VEGF(Nb35) PAGER(FL)

   Plasmid

   #230025

   Purpose

   Expresses anti-VEGF PAGER(FL) in mammalian cells

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   pAAV-MT3-Nb35-TEVcs-ALFA-hM4DxcpGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-hSyn-anti-mCherry(LaM6) PAGER(Gq)

   Plasmid

   #230020

   Purpose

   AAV vector expressing anti-mCherry PAGER(Gq) in neurons

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   MT1-LaM6-TEVcs-ALFA-hM4D
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-UAS-anti-CD3/anti-CD19 BiTE

   Plasmid

   #230005

   Purpose

   Gal4-dependent expression of secreted BiTE

   Depositor

   Alice Ting

   Article

   Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

   Insert

   UAS-anti-CD3/anti-CD19 BiTE
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UAS

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-anti-GFP(LaG16) PAGER(Gs)

    Plasmid

    #230007

    Purpose

    Expresses anti-GFP PAGER(Gs) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    MT1-LaG16-TEVcs-ALFA-hM1Dxs
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-anti-mCherry(LaM6) PAGER(Gq)

    Plasmid

    #230010

    Purpose

    Expresses anti-mCherry PAGER(Gq) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    MT1-LaM6-TEVcs-ALFA-hM1D
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    W442A on hM1D

    Promoter

    CMV

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-anti-GFP(LaG16) PAGER(G12)

    Plasmid

    #230009

    Purpose

    Expresses anti-GFP PAGER(G12) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    MT1-LaG16-TEVcs-ALFA-hM1Dx12
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ibpAB-GFP

    Plasmid

    #109390

    Purpose

    GFP cassette under the control of the ibpAB sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pibpAB-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. htpG1-GFP

    Plasmid

    #109387

    Purpose

    GFP cassette under the control of the htpG1 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    phtpG1-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMZ-347

    Plasmid

    #195458

    Purpose

    Lentiviral Integratable, constitutive expression of synZiFTR: ZF10-p65

    Depositor

    Ahmad Khalil

    Article

    Li et al Science. 2022 Dec 16;378(6625):1227-1234

    Insert

    pSFFV - 3XFLAG - NLS - ZF1 - p65
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EK0285 SFFV-mCherry-SG(s70587)SG-EGFP (FLP-IN)

    Plasmid

    #191140

    Purpose

    Expression of Sensor 1 (for EK0208) in mammalian cells; mCherry marker, EGFP output.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s70587:EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. htpG2-GFP

    Plasmid

    #109388

    Purpose

    GFP cassette under the control of the htpG2 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    phtpG2-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLentiGuide_mCherry_sgEPAS1#1

    Plasmid

    #183122

    Purpose

    pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    sgEPAS1-1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMC-6-ZmBbm-P2A-ZmWus2-9

    Plasmid

    #197750

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    ZmBbm-P2A-ZmWus2 bicistronic CDS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5-mFzd3

    Plasmid

    #42255

    Depositor

    Chris Garcia, Jeremy Nathans

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 3 (Fzd3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pLentiCRISPR_Puro_sgAAVS1

    Plasmid

    #187818

    Purpose

    pLentiCRISPR that is selectable with puromycin with an sgRNA targeting AAVS1

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    sgAAVS1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKLBac-FluB-trimer

    Plasmid

    #229859

    Purpose

    Influenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)

    Depositor

    Stephen Cusack

    Article

    Reich et al Nature. 2014 Dec 18;516(7531):361-6. doi

    Inserts

    PA, Influenza B (B/Memphis/13/03)
    PB1, Influenza B (B/Memphis/13/03)
    PB2, Influenza B (B/Los Angeles/1/02)
    

    Use

    Tags

    2x-Streptag and 8xHis-tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pVC

    Plasmid

    #175063

    Purpose

    expresses mVenus and mCherry pair in mammalian cells

    Depositor

    Arnold Boersma

    Article

    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

    Insert

    mVenus + mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

    Promoter

    CMV Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pARF6-YFP

    Plasmid

    #11389

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    ADP Ribosylation Factor 6 (ARF6 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pWM_12x601_193 NRL + TetO

    Plasmid

    #157812

    Purpose

    EcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomes

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    12x601_tetO
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. INS-2A-luciferase-2A-tdT

    Plasmid

    #159348

    Purpose

    Human INS targeting vector for knockin of 2A separated luciferase and tdTomato. Targeted cells will be puromycin resistant.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

    Insert

    Luciferase-2A-tdTomato
    

    Use

    Ins locus knockin donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRK5_tAKARβ

    Plasmid

    #119914

    Purpose

    Mammalian expression of the tAKARβ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARβ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEM705 Gst (Ser)

    Plasmid

    #109354

    Purpose

    GsX chimera with Gαt C-terminus (Pertussis toxin insensitive mutant)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gst chimera
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Changed Cysteine at position 391 to Serine (Pertu…

    Promoter

    CAG

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJYS1Peftu

    Plasmid

    #85546

    Purpose

    Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Inserts

    Cpf1
    recT
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    etfu and unknown

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUPD2

    Plasmid

    #68161

    Purpose

    Universal domesticator plasmid for level 0 parts

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pcDNA3-NP-B/BRISBANE/60/2008

    Plasmid

    #187921

    Purpose

    Expresses nucleoprotein of Influenza B/Brisbane/60/2008 virus in mammalian cells

    Depositor

    Arindam Mondal

    Article

    Kedia et al Front Microbiol. 2022 May 25;13:868367.

    Insert

    Influenza B Nucleoprotein
    

    Use

    Tags

    UNTAGGED

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. GFP/KATNA1

    Plasmid

    #89316

    Purpose

    Expression of rat GFP/Katanin A subunit in mammalian cells

    Depositor

    Peter Baas

    Article

    Karabay et al J Neurosci. 2004 Jun 23;24(25):5778-88.

    Insert

    KATNA1 (Katna1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Shp2 biosensor

    Plasmid

    #134346

    Purpose

    FRET biosensor. To visualize Shp2 activity by FRET

    Depositor

    Yingxiao Wang

    Article

    Sun et al Nat Commun. 2017 Sep 7;8(1):477. doi: 10

    Insert

    Shp2 Biosensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Flag Snail 6SA

    Plasmid

    #16221

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

    Insert

    Snail (SNAI1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S96A, S100A, S107A, S111A, S115A, S119A

    Promoter

    Available since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSMART-SOS-GFPuv

    Plasmid

    #102283

    Purpose

    Fluorescent sensor of E. coli SOS response

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    cda-GFPuv
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cda SOS promoter

    Available since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAID-EF1a linearizing in pX330

    Plasmid

    #140610

    Purpose

    Crispr/Cas9 plasmid for linearization of pAID-EF1a

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    sgRNA for pAID-EF1a plasmid digestion
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJV53-Cas12a

    Plasmid

    #158706

    Purpose

    CRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetO

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pTE5400_pMBP_CDS dropout vector

    Plasmid

    #221189

    Purpose

    Expression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interest

    Depositor

    Tobias Erb

    Article

    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

    Type

    Empty backbone

    Use

    Tags

    10x His-tag + E. coli maltose binding protein (MB…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pPBbsr2-Lifeact-miRFP703

    Plasmid

    #178530

    Purpose

    Expresses Lifeact-miRFP703 in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

    Insert

    Lifeact-miRFP703
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLKO.1P shACO1

    Plasmid

    #169884

    Purpose

    Suppress ACO1

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    shRNA against ACO1
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge