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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 10401 - 10450 of 12660 results

  5. pLaNGo11-7

    Plasmid
    #126055
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x7 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    126055-plasmid-map-sequence-id-247152

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  20. pJC.15A.sgRNA.TA

    Plasmid
    #232483
    Purpose
    sgRNA cloning backbone for expression of sgRNA in C. difficile. Contains TA system to enable maintenence without antibiotic selection.
    Depositor
    James Collins
    Article
    Chua et al Microbiol Resour Announc. 2025 Apr 28:e0
    Inserts
    Toxin-antitoxin System from CD630
    medium-copy-number p15A origin of replication
    mCherry
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    P3 constitutive promoter and native promoter
    Available Since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    232483-plasmid-map-sequence-id-466415

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  27. pBAD-tdTEVDB_C-15A/G4C

    Plasmid
    #177205
    Purpose
    Permuted td intron with evolved initiation sequence for the expression of circular mRNA
    Depositor
    Stephen Fried
    Article
    Lee et al ACS Cent Sci. 2021 Oct 27;7(10):1736-175
    Insert
    Permuted GFP with evolved initiation sequence for circular mRNA
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Permuted sfGFP after residue 52 & Changed Cyt…
    Promoter
    araBAD
    Available Since
    Sept. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177205-plasmid-map-sequence-id-373215

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  28. pAC-DELTA

    Plasmid
    #53273
    Purpose
    Contains crtE, crtB, and crtI genes of Erwinia herbicola (Pantoea agglomerans) Eho10, together with the lcyE gene of Arabidopsis thaliana and thereby produces delta-carotene in E. coli
    Depositor
    Francis X Cunningham Jr
    Article
    Sun et al J Biol Chem. 1996 Oct 4;271(40):24349-52
    Insert
    lcyE (LUT2 Mustard Weed)
    Use
    Low copy number bacterial cloning vector
    Promoter
    Trc
    Available Since
    Jan. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    53273-plasmid-map-sequence-id-84075

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  39. pSLC-255

    Plasmid
    #124292
    Purpose
    Expression of vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.
    Depositor
    Swaine Chen
    Article
    Eshaghi et al Angew Chem Int Ed Engl. 2015 Nov 16;54(4
    Insert
    vsfGFP-9
    Expression
    Bacterial
    Available Since
    Sept. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    124292-plasmid-map-sequence-id-243084

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  40. pMpGWB114

    Plasmid
    #68568
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Tags
    Glucocorticoid receptor (GR)
    Expression
    Plant
    Promoter
    Cauliflower mosaic virus 35S promoter
    Available Since
    July 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    68568-plasmid-map-sequence-id-121730

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  48. pLC133

    Plasmid
    #198639
    Purpose
    Expression plasmid for dCas9 in E. coli used in Chip-seq experiments. dCas9 is tagged with a C-terminal 3x FLAG tag. A guide RNA can be cloned using BsaI restriction sites.
    Depositor
    David Bikard
    Article
    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.
    Insert
    dCas9
    Tags
    3x-FLAG
    Expression
    Bacterial
    Promoter
    pTet
    Available Since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198639-plasmid-map-sequence-id-390787

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Showing: 10401 - 10450 of 12660 results