We narrowed to 31,602 results for: REP

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7h sgRNA for 4-μHOM reporter

Plasmid

#113625

Purpose

sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter at the edge of the microhomology.

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7h sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
betaTrCP mVenus reporter

Plasmid

#192458

Purpose

Fluorescent reporter for beta-TrCP activity in human cells

Depositor

Steven Cappell

Article

Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

Insert

CDC25B (CDC25B Human)

Use

Lentiviral

Tags

SV40 NLS and mVenus

Expression

Mammalian

Mutation

aa198-338

Promoter

EF1alpha

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pXT131_TwinStrep-SUMO-ShTniQ

Plasmid

#135527

Purpose

Protein expression construct for ShTniQ

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTniQ

Tags

TwinStrep-SUMO

Expression

Bacterial

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-Npl4-Strep-HA

Plasmid

#113495

Purpose

Expression of human Npl4 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

Npl4 (NPLOC4 Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pXT129_TwinStrep-SUMO-ShTnsB

Plasmid

#135525

Purpose

Protein expression construct for ShTnsB

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTnsB

Tags

TwinStrep-SUMO

Expression

Bacterial

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-Ufd1-Strep-HA

Plasmid

#113474

Purpose

Expression of human Ufd1 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

Ufd1 (UFD1 Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pIgK-2.1-StreptagII

Plasmid

#225830

Purpose

Mouse IgK chain specific for chicken gamma globulin (clone 2.1). Used to produce StreptagII-tagged monoclonal antibodies for use as tag-specific ELISA standards.

Depositor

Gabriel Victora

Article

Schiepers et al Nature. 2023 Mar;615(7952):482-489. doi:

Insert

Mouse anti-CGG IgK chain, Streptag-II-tagged

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pFsynW SYT1 reporter

Plasmid

#197275

Purpose

Encodes for viral expression of wild type Synaptotagmin 1 (SYT1) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYT1 and HaloTag (Syt1 Rat)

Use

Lentiviral

Tags

FLAG

Mutation

N/A

Promoter

hSyn (human synapsin I promoter)

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pTLR repair vector

Plasmid

#64322

Purpose

Vector for repair of the traffic light reporter (TLR), providing the venus coding region

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Insert

Venus

Use

Artificial targeting vector

Mutation

see publication for details

Promoter

none

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF8-2xCSTREP

Plasmid

#136172

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF8

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF8

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K8.1-2xCSTREP

Plasmid

#136157

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K8.1

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K8.1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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FL MALT1 WT in pET29b-Strep

Plasmid

#48968

Purpose

Expresses Full length wild type MALT1 in e.coli

Depositor

Guy Salvesen

Article

Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

Insert

MALT1 isoform A (MALT1 Human)

Tags

His and Strep

Expression

Bacterial

Promoter

T7

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only
pPJ23116-MS2-rep

Plasmid

#184842

Purpose

Expression of the rep protein of phage MS2

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Replicase gene MS2 phage

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23116

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-StrepHA-SUMO2

Plasmid

#66867

Purpose

Mammalian expression of SUMO2 with a strep and an HA tag

Depositor

Ying Liu

Article

Bursomanno et al J Proteome Res. 2015 Apr 3;14(4):1687-99

Insert

SUMO2 (SUMO2 Human)

Tags

HA and Strep

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pmiR-report-MYB-WT

Plasmid

#141114

Purpose

Reporter plasmid of WT MYB in mammalian cells.

Depositor

Jianjun Chen

Article

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

CMYB (MYB Human)

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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gRNA-eGFP-Reporter

Plasmid

#60719

Purpose

Expresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

gRNA (5'-AAAGGTCGAGAAACTGCAAA-3')

Use

CRISPR

Expression

Mammalian

Promoter

U6 and mouse U6

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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mCh-KIF1A*-strep

Plasmid

#120165

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF1A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 1A (Kif1a Mouse)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF1A: 1-405 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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PME-1 pcDNA-StrepIII

Plasmid

#119292

Purpose

Mammalian overexpression of StrepII-tagged full length PPME1

Depositor

Jukka Westermarck

Article

Pokharel et al Mol Cell Proteomics. 2015 Dec;14(12):327

Insert

PPME1 (PPME1 Human)

Expression

Mammalian

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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Drp1-EGFP-StrepII

Plasmid

#174429

Purpose

Expresses human Drp1 Isoform 3 with EGFP at C-terminus in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Tags

EGFP-StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pnCS_SEPT7_SEPT9_i5-TEV-Strep

Plasmid

#174502

Purpose

bacterial co-expression of human SEPT7 and of human SEPT9_i5

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN9 (SEPTIN9 Human)

Tags

TEV-Strep

Expression

Bacterial

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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