175,825 results

1. 8522-M31-663

   Plasmid

   #225661

   Purpose

   Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (red configuration)

   Depositor

   Dominic Esposito

   Article

   Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

   Insert

   dsCherry
   

   Use

   Lentiviral

   Promoter

   SORE6-mCMVp

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(-)-HA-SrtAstrep (pKS77)

   Plasmid

   #50030

   Purpose

   Expression of cytosolic S. pyogenes SrtA in mammalian cells

   Depositor

   Hidde Ploegh

   Article

   Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

   Insert

   SrtA(strep)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE

   Plasmid

   #125712

   Purpose

   Expresses an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

   Depositor

   Ofer Yizhar

   Article

   Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

   Has Service

   AAV Retrograde, AAV1, and AAV5

   Insert

   eOPN3
   

   Use

   AAV

   Tags

   Rho1D4 and mScarlet

   Expression

   Mammalian

   Mutation

   deleted amino acids 331-429

   Promoter

   short version of αCamKinase II promoter (0.4kb)

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-HA-TRAF3IP3 (mouse)

   Plasmid

   #71459

   Purpose

   Inducible expression of TRAF3IP3 in mammalian cells

   Depositor

   Shao-Cong Sun

   Article

   Zou et al J Exp Med. 2015 Jul 27;212(8):1323-36. d

   Insert

   TRAF3IP3 (Traf3ip3 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA5FRT/TO-SAKS1-Strep-HA

   Plasmid

   #113487

   Purpose

   Expression of human SAKS1 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   SAKS1 (UBXN1 Human)
   

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(30-120)

   Plasmid

   #191391

   Purpose

   Donor for targeted integration of hCdt1(30-120) probe to the hROSA26 safe harbor locus

   Depositor

   Yannick Doyon

   Insert

   hCdt1(30-120)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   mScarlet-I

   Expression

   Mammalian

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHS0806 His14-bdSUMO-AwaIscB

   Plasmid

   #176539

   Purpose

   Bacterial expression of His14-bdSUMO-AwaIscB

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   AwaIscB
   

   Use

   CRISPR

   Tags

   14xHis-bdSUMO tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3 Flag Fli1

   Plasmid

   #66976

   Purpose

   Expression plasmid for mouse Fli1; adds Flag tag to the N-terminus of protein

   Depositor

   Christopher Vakoc

   Article

   Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

   Insert

   Fli1 (Fli1 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

9. TRUPATH Triple GaoB

   Plasmid

   #196052

   Purpose

   Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphaoB-RLuc8
   GGamma8-GFP2 (GNG8 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-C1-ER alpha

    Plasmid

    #28230

    Purpose

    Mammalian expression of Estrogen Receptor alpha fused to EGFP

    Depositor

    Michael Mancini

    Article

    Stenoien et al Mol Endocrinol. 2000 Apr . 14(4):518-34.

    Insert

    Estrogen Receptor alpha (ESR1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. NEK7_HUMAN_D0

    Plasmid

    #79735

    Purpose

    This plasmid encodes the kinase domain of NEK7. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, John Chodera, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    NEK7 (NEK7 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

12. TFORF2043

    Plasmid

    #142316

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ARID3A (ARID3A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBMN-dGBP1-TagBFP

    Plasmid

    #80087

    Purpose

    MMLV retroviral vector for expression of dGBP1-TagBFP with t-hcRed as an infection/transfection marker

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Insert

    dGBP1-TagBFP
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7-EGFP-C1-HsNXF-1_K

    Plasmid

    #146740

    Purpose

    Mammalian Expression of HsNxf-1

    Depositor

    Elisa Izaurralde

    Article

    Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

    Insert

    HsNxf-1 (NXF1 Human)
    

    Expression

    Mammalian

    Mutation

    S144G relative to NM_006362.4

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYLCRISPR/Cas9Pubi-H

    Plasmid

    #66187

    Purpose

    expression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    maize ubiquitin promoter (Pubi)

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHD-DsRed

    Plasmid

    #51434

    Purpose

    Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed.

    Depositor

    Kate O'Connor-Giles

    Article

    Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

    Insert

    LoxP-3xP3-DsRed-LoxP
    

    Promoter

    3xP3

    Available Since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGM

    Plasmid

    #71250

    Purpose

    luciferase reporter with human GM-CSF promoter -627 to + 28

    Depositor

    Peter Cockerill

    Article

    Cockerill et al Proc Natl Acad Sci U S A. 1999 Dec 21;96

    Insert

    GM-CSF promoter -627 to +28 fragment (CSF2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    GM-CSF

    Available Since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJO672 (pRS415 Gal1-Ufd4-flag)

    Plasmid

    #159590

    Purpose

    expresses Ufd4-flag in yeast cells

    Depositor

    Alexander Varshavsky

    Insert

    Ufd4
    

    Expression

    Yeast

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 T21R-vhhGFP4-T2A-mCherry

    Plasmid

    #225587

    Purpose

    Mammalian expression vector for TRIM21 RING-vhhGFP4 anti-GFP degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

    Depositor

    William McEwan

    Article

    Benn et al Science. 2024 Aug 30;385(6712):1009-1016

    Insert

    T21R-vhhGFP4-T2A-mCherry
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBR HPV31 9E

    Plasmid

    #221009

    Purpose

    Human papillomavirus 31 isolate 9E, complete genome cloned in pBR322

    Depositor

    Alison McBride

    Insert

    Human papillomavirus type 31 genome 9E isolate
    

    Use

    Cloning vector

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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