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HDM_RSV_Long_G_31AACTdel

Plasmid

#237350

Purpose

Expression plasmid for RSV Long G

Depositor

Jesse Bloom

Article

Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

Insert

RSV Long G from Genbank Accesion AY911262.1

Expression

Mammalian

Mutation

31 amino acid cytoplasmic tail deletion

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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HDM_RSV_Long_F

Plasmid

#237349

Purpose

Expression plasmid for RSV Long F

Depositor

Jesse Bloom

Article

Simonich et al J Virol. 2025 Jul 3:e0053125. doi: 10.11

Insert

RSV Long F from Genbank Accesion AY911262.1

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pQE30-roGFP1-iR

Plasmid

#83314

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

Insert

roGFP1-iR

Tags

6xHIS

Expression

Bacterial

Mutation

ro1(C48S/S147C/Q204C) plus X insertion, iR betwe…

Promoter

T5

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
pmCherry-T2A-moxMaple3

Plasmid

#120875

Purpose

Mammalian expression of mCherry and moxMaple3

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

Inserts

mCherry
moxMaple3

Expression

Mammalian

Mutation

from mMaple3, moxMaple3 was engineered by replaci…

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 alpha-actinin 1

Plasmid

#11908

Depositor

Carol Otey

Article

Edlund et al Cell Motil Cytoskeleton. 2001 Mar . 48(3

Insert

alpha-actinin 1 (ACTN1 Human)

Tags

eGFP

Expression

Mammalian

Available Since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

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pCDH-puro-CMV-GC3AI

Plasmid

#78910

Purpose

Indicator of caspase-3-like protease activity

Depositor

Binghui Li

Article

Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

Insert

GC3AI

Use

Lentiviral

Tags

HA and Myc

Mutation

S320A, deletion of AA1-200

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pC1-HyPer-Red-C199S

Plasmid

#48252

Purpose

Hydrogen peroxide insensitive version of HyPerRed to be used as a pH control in HyPer experiments.

Depositor

Vsevolod Belousov

Article

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

HYPER-RED-C199S

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pDAS12395_PEAR-GFP_2.0-preedited

Plasmid

#177185

Purpose

PEAR-GFP3 control plasmid with split EGFP and intact 5' splice site

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pNCS-mClover3

Plasmid

#74236

Purpose

Constitutive expression of mClover3 in bacteria

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Has Service

Cloning Grade DNA

Insert

mClover3

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-mCherry-CAAX

Plasmid

#129285

Purpose

Lentiviral plasmid for expression of fluorescent reporter mCherry targeted to the plasma membrane via CAAX polybasic sequence

Depositor

Carlos Carmona-Fontaine

Article

Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

Insert

Fluorescent reporter mCherry fused to CAAX polybasic sequence

Use

Lentiviral

Tags

CAAX

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRU324

Plasmid

#167691

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH_SBP-mCherry-GPI

Plasmid

#65299

Purpose

synchronize trafficking of mCherry-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Use

Lentiviral

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-Ecadherin

Plasmid

#65286

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-PKCA WT

Plasmid

#195202

Purpose

Mammalian expression vector for GFP tagged PKCA.

Depositor

David Bryant, Peter Parker

Article

Cameron et al Nat Struct Mol Biol. 2009 Jun;16(6):624-

Insert

PRKCA (PRKCA Human)

Expression

Mammalian

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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ZJOM31

Plasmid

#133645

Purpose

Integration of TGL3-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast lipid droplet.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-TGL3-GFP (TGL3 Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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ZJOM90

Plasmid

#133647

Purpose

Integration of SS-mCherry-HDEL in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast endoplasmic reticulum (ER).

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

pTPI1-SS-mCherry-HDEL

Tags

mCherry

Expression

Yeast

Available Since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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pHybrid-Electra2

Plasmid

#179475

Purpose

Expresses Electra2 in bacterial cells and Electra2 and mScarlet in mammalian cells

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Inserts

Electra2
mScarlet-I

Expression

Bacterial and Mammalian

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMP31-1

Plasmid

#45905

Depositor

Harmit Malik

Article

Patel et al PLoS Biol. 2012;10(3):e1001282. doi: 10.

Insert

mitochondrial antiviral signaling protein (MAVS Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pET21d-myc-BirA

Plasmid

#109424

Purpose

Bacterial expression of biotin ligase (BirA) with an N-terminal myc epitope

Depositor

Martin Spiess

Article

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

BirA (birA )

Tags

myc

Expression

Bacterial

Promoter

T7

Available Since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-puro-CMV-CC3AI

Plasmid

#78909

Purpose

Indicator of caspase-3-like protease activity

Depositor

Binghui Li

Article

Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

Insert

CC3AI

Use

Lentiviral

Tags

HA and Myc

Mutation

S320A, deletion of AA1-200

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pmScarlet3-H_I3

Plasmid

#239343

Purpose

Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3-H in mammalian cells. Assembles into nanocages tagged with 60 FPs.

Depositor

Torsten Wittmann

Article

Viola et al J Cell Sci. 2025 May 15;138(10):jcs26385

Insert

I3-01

Tags

mScarlet3-H (a.k.a. mYongHong)

Expression

Mammalian

Mutation

K129A

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaBC (pCLK1210)

Plasmid

#50448

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylase

Depositor

Tadhg Begley, Erick Strauss

Article

Strauss et al J Biol Chem. 2001 Apr 27;276(17):13513-6

Insert

PPCS PPCDC

Tags

6XHIS tag

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2014

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-Giantin

Plasmid

#129630

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

mTurquoise2-Giantin

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pKL003

Plasmid

#98919

Purpose

Constitutively expresses the voltage indicator PROPS under the Sigma 70 promoter in E. coli.

Depositor

Joel Kralj

Article

Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

Insert

PROPS

Tags

His

Expression

Bacterial

Promoter

BBa_J23118 - Strong Constitutive

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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plenti.CamKII.(cyto).iATPSnFR2.A95K.S29W.miRFP670nano3

Plasmid

#209728

Purpose

Expresses cytosolic high affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(cyto).iATPSnFR2.A95K.S29W.miRFP670nano3

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pR-mAmetrine

Plasmid

#228598

Purpose

Mammalian expression of mAmetrine, for MuSIC barcode construction

Depositor

Marc Birtwistle

Article

Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

Insert

mAmetrine

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-E2-Crimson

Plasmid

#111252

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with E2-Crimson

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

E2-Crimson

Expression

Bacterial

Promoter

Pc

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pETM6-HpaBC

Plasmid

#71260

Purpose

Expresses E. coli HpaBC in monocistronic form from ePathBrick vector, pETM6

Depositor

Mattheos Koffas

Article

Jones et al Biotechnol Prog. 2015 Oct 21. doi: 10.10

Inserts

HpaB
HpaC

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

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SepOTSλ

Plasmid

#68292

Purpose

Phosphoserine orthogonal translation system (preferred variant)

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EFSep21, 4xtRNA-Sep-A37

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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mScarlet-I-PXN

Plasmid

#112956

Purpose

In vivo visualization of focal adhesions (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

PXN

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_flGalT-SBP-mCherry

Plasmid

#65273

Purpose

synchronize trafficking of GalT from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

galactosidase

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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EBFP2-Mito-7

Plasmid

#55248

Purpose

Localization: Mitochondria, Excitation: 383, Emission: 448

Depositor

Michael Davidson

Article

Shaner et al J Cell Sci. 2007 Dec 15;120(Pt 24):4247-

Insert

Mito (COX8A Human)

Tags

EBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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3xmNeonGreen_UtrCH

Plasmid

#129607

Purpose

In vivo visualization of F-actin (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Utrophin

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CMVie-NcanGFP

Plasmid

#235983

Purpose

Expresses the hyaluronan-binding domain of Neurocan fused to eGFP under the CMV promoter. It contains a sequence for secretory pathway, so the probe is exported to the extracellular space

Depositor

Federico Soria

Article

Fernández-Ballester et al bioRxiv 2025.01.31.635882; doi: 10.1101/

Insert

Neurocan-GFP

Use

AAV

Tags

GFP

Promoter

CMVie

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-H13LTat-CD8a/d2eGFP-IRES-Nef

Plasmid

#126552

Purpose

Replication incompetent HIV with H13L Tat and d2eGFP/CD8a reporter

Depositor

Jonathan Karn

Article

Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

Insert

HIV-1 vector pNL4-3

Use

Lentiviral

Tags

CD8a and GFP

Mutation

Delta gag/pol

Promoter

HIV LTR

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCytERM-mTagBFP2

Plasmid

#184940

Purpose

ER Visualization (OSER assay) Expresses mTagBFP2 in fusion with N-terminal part of p450

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

CytERM-mTagBFP2

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miniGFP1-P2A-FusionRed

Plasmid

#188732

Purpose

Express miniGFP1 and FusionRed in mammalian cells

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP1

Use

AAV

Tags

FusionRed

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCalN-ddGFP-A

Plasmid

#40290

Depositor

Robert Campbell

Article

Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

Insert

Calnexin (CANX Human)

Tags

ddGFP-A

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.iGABASnFR

Plasmid

#112172

Purpose

GABA Sensor

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

Insert

iGABASnFR

Use

AAV

Promoter

GFAP

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pBad-mCherry-XL (R125K)

Plasmid

#188054

Purpose

Expresses His-tagged, Arabinose-inducible mCherry-XL (R125K) in bacteria

Depositor

Ralph Jimenez

Article

Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

Insert

mCherry-XL (R125K)

Tags

6xHis

Expression

Bacterial

Mutation

differs from mCherry by R125K, W143S, I161V, Q163…

Promoter

araBAD

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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