Skip to main content
Addgene
Showing: 6481 - 6510 of 8440 results

  2. Halo-SHLD2 HRD

    Plasmid
    #207078
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous SHLD2 locus.
    Depositor
    Jens Schmidt
    Article
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Insert
    HaloTag with internal PuroR cassette flanked by human SHLD2 locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    207078-plasmid-map-sequence-id-431782

    Enlarge

  8. SNAP-LC3 HRD

    Plasmid
    #207545
    Purpose
    Homologous recombination donor to integrate a SNAP-Tag at the endogenous LC3 locus in human cells
    Depositor
    Jens Schmidt
    Article
    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007
    Insert
    SNAPtag with internal PuroR cassette flanked by human LC3B locus sequences
    Use
    Tags
    SNAPtag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    207545-plasmid-map-sequence-id-430987

    Enlarge

  9. Halo-LC3B HRD

    Plasmid
    #207539
    Purpose
    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous LC3B locus in human cells
    Depositor
    Jens Schmidt
    Article
    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007
    Insert
    HaloTag with internal PuroR cassette flanked by human LC3B locus sequences
    Use
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous
    Available since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    207539-plasmid-map-sequence-id-429514

    Enlarge

  12. INT_Cargo_T7_gfp

    Plasmid
    #212136
    Purpose
    Cargo plasmid for integrating PT7 expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Article
    Fung et al Biomaterials. 2022 Aug;287:121661. doi:
    Insert
    green fluorescent protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212136-plasmid-map-sequence-id-420767

    Enlarge

  13. INT_Cargo_T7_CbFDH

    Plasmid
    #212140
    Purpose
    Cargo plasmid for integrating PT7 expression cassette of formate dehydrogenase in genome. Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Article
    Fung et al Biomaterials. 2022 Aug;287:121661. doi:
    Insert
    formate dehydrogenase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212140-plasmid-map-sequence-id-420766

    Enlarge

  26. NK809

    Plasmid
    #196406
    Purpose
    Strong expression of firefly and nanoluc luciferases codon-optimized for S. rosetta
    Depositor
    Nicole King
    Article
    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092
    Insert
    Sro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    Use
    Luciferase
    Tags
    Expression
    Mutation
    N/A (synthetic)
    Promoter
    Available since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    196406-plasmid-map-sequence-id-385017

    Enlarge

  27. NK810

    Plasmid
    #196407
    Purpose
    Nanoluc luciferase under S. rosetta SPAG6 promoter with internal actin-firefly control
    Depositor
    Nicole King
    Article
    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092
    Insert
    Sro5spag6-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    Use
    Luciferase
    Tags
    Expression
    Mutation
    N/A (synthetic)
    Promoter
    Available since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    196407-plasmid-map-sequence-id-385018

    Enlarge

  28. NK811

    Plasmid
    #196408
    Purpose
    Nanoluc luciferase under S. rosetta SPAG6 promoter with mutated RFX binding site with internal actin-firefly control
    Depositor
    Nicole King
    Article
    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092
    Insert
    Sro5spag6_mut-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    Use
    Luciferase
    Tags
    Expression
    Mutation
    N/A (synthetic)
    Promoter
    Available since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    196408-plasmid-map-sequence-id-384995

    Enlarge

  30. pL1P6 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198049
    Purpose
    MoClo Golden Gate Level 1 Position 6. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    John Innes Centre - Wheat Transformation and Genome Editing, Cristobal Uauy
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198049-plasmid-map-sequence-id-391867

    Enlarge

Showing: 6481 - 6510 of 8440 results