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pF
Plasmid#42519DepositorTypeEmpty backboneUseUnspecified; CloningTagsExpressionBacterialMutationPromoterT7, T3Available sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lenti sgRNA CFP
Plasmid#155281PurposeLentiviral sgRNA cloning with CFP markerDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWS931
Plasmid#123053PurposeTetR bacterial repressorDepositorInsertTetR-NLS [3a]
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
HR_G3BP1-V5-APEX2-GFP
Plasmid#105284Purposehomologous recombination donor top insert V5-APEX2-GFP into the C-terminus of the human G3BP1 locusDepositorInsertG3BP1 homology arms flanking V5-APEX2-GFP and EF1a-puro selection cassette (G3BP1 soybean, Human)
UseHuman homologous recombination donorTagsExpressionMutationPromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
PRKCE gRNA (BRDN0001148049)
Plasmid#76791Purpose3rd generation lentiviral gRNA plasmid targeting human PRKCEDepositorInsertPRKCE (PRKCE Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP
Plasmid#31484DepositorInsertEF1s HA NLS Sce(opt) T2A IFP1.4
UseLentiviralTagsHA and NLSExpressionMutation"Opt" indicates the I-SceI coding seque…PromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
SPHK1 gRNA (BRDN0001147454)
Plasmid#76813Purpose3rd generation lentiviral gRNA plasmid targeting human SPHK1DepositorInsertSPHK1 (SPHK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRFAM7A-YFP
Plasmid#62634Purposemammalian expression of human duplicated Alpha7 (CHRFAM7A) with YFP fused into M3-M4 loopDepositorInsertCHRFAM7A (CHRFAM7A Human)
UseTagsExpressionMammalianMutationYFP inserted in M3-M4 loopPromoterCMVAvailable sinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pB-CMPE-ePPEplus
Plasmid#205246PurposeFor plant prime editing in wheat plantsDepositorInsertCsy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRTagsExpressionPlantMutationDetailed in manuscriptPromoterAvailable sinceMay 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MCP-dNS-SRRM1-V5-mCherry
Plasmid#235095PurposeSRRM1 lacking Nuclear Speckle domain (dNS-SRRM1) mCherry fusion protein with MCP domainDepositorInsertpCAG-MCP-dNS-SRRM1-V5-mCherry (SRRM1 Human)
UseTagsExpressionMammalianMutationThe SRRM1 entry clone from DNASU was modified usi…PromoterCAGAvailable sinceMay 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
G3BP1-Grx1-roGFP2
Plasmid#236548PurposeExpressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in stress granules.DepositorInsertG3BP1-Grx1-roGFP2 (G3BP1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
NoLS-Grx1-roGFP2
Plasmid#236545PurposeExpressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the nucleoli.DepositorInsertNoLS-Grx1-roGFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
Grx1-roGFP2-Dcp1b
Plasmid#236547PurposeExpressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in processing bodies.DepositorInsertGrx1-roGFP2-Dcp1b (DCP1B Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
Grx1-roGFP2-Coilin
Plasmid#236546PurposeExpressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in Cajal bodies.DepositorInsertGrx1-roGFP2-Coilin (COIL Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
XZ091 (PB) CMV-sCCL20 (PuroR-BFP)
Plasmid#233434PurposePiggyBac donor plasmid, secreted CCL20DepositorInsertCCL20 (CCL20 Human)
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DjCas13d-HA-mCherry-pA
Plasmid#233030PurposeTo Express HA tagged DjCas13d-mCherry fusion protein from the mammalian EFS promoterDepositorInsertDjCas13d-mCherry
UseAAVTagsmCherryExpressionMutationPromoterAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXJ-Nanoluc (Nluc)
Plasmid#232656PurposeNanoluc luciferase expression driven CMV promoterDepositorInsertNanoluc
UseLuciferaseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_CD63-SBP-mCherry
Plasmid#222326PurposeSynchronize the trafficking of CD63 from the ER.DepositorInsertStreptavidin-KDEL and CD63 fused to SBP-mCherry (CD63 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
Anti-Gephyrin [L106/83-rat IgG2a] - Chimeric
Plasmid#227048PurposeMammalian expression plasmid of anti-Gephyrin (Homo sapiens) rat IgG2a R-mAb. Derived from hybridoma L106/83.DepositorInsertAnti-Gephyrin (Homo sapiens) recombinant mouse monoclonal antibody. (GPHN Chimera: M. musculus (mouse)/R. norvegicus (rat))
UseAffinity Reagent/ AntibodyTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7058
Plasmid#167227Purposeoverexpression of orthogonal T7 RNAPDepositorInsertJ23119-AKSIRV
UseSynthetic BiologyTags6X HisExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERH-ZNF281
Plasmid#222923PurposePiggyBac transposon plasmid for doxycycline inducible expression of ZNF281DepositorInsertZNF281 (ZNF281 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
SPLICSFAST-ER-PO-P2A
Plasmid#214894PurposeExpresses equimolar Peroxisome- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PO Contact sitesDepositorInsertSPLICSFAST-ER-PO-P2A
UseTagsnoneExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
GW1-rogRFP2
Plasmid#207876PurposeMammalian expression of the red fluorescent rogRFP2 redox sensorDepositorInsertrogRFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
HE_150
Bacterial Strain#201051PurposeDH10B-derived Escherichia coli isolate obtained from adaptive laboratory evolution to increasing incubation temperatures.DepositorBacterial ResistanceStreptomycinAvailable sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hEPR
Plasmid#199233PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertProepiregulin preproprotein (EREG Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas12a
Plasmid#186446PurposeEncodes Cas12a from F. novicida (type V-A)DepositorInsertCas12a
UseCRISPRTagsExpressionMutationPromoterT7Available sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pILGFP5EA4
Plasmid#185879PurposeA plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (4 copy)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMYT017
Plasmid#180670PurposeEncodes LacI as a Type 3 part to be used in the Dueber YTK and Ellis MYT systemsDepositorInsertLacI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-Mito-pHCountdown
Plasmid#182302Purposelentiviral plasmid encoding pH-Countdown targeted to mitochondria via fusion to the COX8A presequence with puromycin selectionDepositorInsertmito pH countdown
UseLentiviralTagsCOX8A mitochondria targeting sequenceExpressionMutationPromoterEF-1aAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only
