167,895 results

1. mscv2.2-6mycFlaA

   Plasmid

   #60203

   Purpose

   6xMYC-tagged full-length FlaA from Legionella pneumophila, expressed under the constitutive, moderate-level retroviral LTR promoter for mammalian cell expression; IRES-GFP reporter downstream of FlaA

   Depositor

   Russell Vance

   Article

   Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

   Insert

   FlaA
   

   Use

   Retroviral

   Tags

   6xMYC

   Expression

   Mammalian

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Anep8_Cas9

   Plasmid

   #117169

   Purpose

   Expressed Cas9 in Aspergillus with LIC tags for easy gRNA cloning

   Depositor

   Adrian Tsang

   Article

   Song et al PLoS One. 2018 Aug 24;13(8):e0202868. do

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology; Filamentous fungi e…

   Promoter

   Pki

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LTN1_pCSdest

   Plasmid

   #53855

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   LTN1 (LTN1 Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   Sept. 9, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pJ204-loxP-EF1a-tagBFP-HygR-lox2272-BGHpA-siteT9-HDR

   Plasmid

   #154827

   Purpose

   Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site T9)

   Depositor

   Lise Marie Grav

   Article

   Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

   Inserts

   TagBFP
   HygR
   ZsGreen1-DR
   

   Use

   CRISPR and Cre/Lox

   Tags

   PEST

   Expression

   Mammalian

   Promoter

   CMV, EF1a, and SV40 early promoter

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Inzolia library in pRDA_550

   Pooled Library

   #209552

   Purpose

   The human, genome-wide, CRISPR knockout enAsCas12a gRNA library targets single genes and paralog pairs, triples, and quads with arrays of 4 gRNAs.

   Depositor

   John Doench

   Article

   Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

   Use

   CRISPR and Lentiviral

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

6. GST-HA-GFP11-N1

   Plasmid

   #91953

   Purpose

   Expresses GST-HA-GFP11 in mammalian cells

   Depositor

   Rong Li

   Article

   Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

   Insert

   GST-HA-GFP11
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCggRO-reporter

   Plasmid

   #124582

   Purpose

   Reporter plasmid with Constitutively expressed mCherry and CggR-regulated YFP (eCitrine)

   Depositor

   Matthias Heinemann

   Article

   Monteiro et al Mol Syst Biol. 2019 Dec;15(12):e9071. do

   Insert

   CggR promoter element
   

   Expression

   Yeast

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GAL2-Tango

   Plasmid

   #66289

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GAL2 (GALR2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pDEST-EGFP-TARG1

   Plasmid

   #172586

   Purpose

   For transient expression of N-terminal GFP-tagged TARG1

   Depositor

   Karla Feijs

   Article

   Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

   Insert

   TARG1 (OARD1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-HAT1_AG

    Plasmid

    #217113

    Purpose

    Mammalian expression vector for expression of HAT1_AG transposase (CMV promoter)

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    HAT1_AG transposase
    

    Expression

    Mammalian

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLVX-EF1alpha-2xGFP:NES-IRES-2xRFP:NLS

    Plasmid

    #71396

    Purpose

    2Gi2R assay

    Depositor

    Fred Gage

    Article

    Mertens et al Cell Stem Cell. 2015 Oct 6. pii: S1934-5

    Inserts

    2xGFP:NES
    2xRFP:NLS
    

    Use

    Lentiviral

    Tags

    IRES

    Expression

    Mammalian

    Promoter

    EF1alpha and IRES

    Available Since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRK5-p18-FLAG

    Plasmid

    #42331

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

    Insert

    p18 (LAMTOR1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV, SP6

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-EF1a-fDIO-Cre (AAV9)

    Viral Prep

    #121675-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Alexander Nectow, Esteban Engel

    Article

    Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

    Promoter

    EF1a

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

14. pcsII-pb5-flag

    Plasmid

    #86769

    Purpose

    C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

    Depositor

    Do-Hyung Kim

    Article

    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

    Insert

    proteasome beta subunit 5 (PSMB5 Human)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Anti-Neurexin-1-Beta [N170A/1.1R]

    Plasmid

    #114530

    Purpose

    Mammalian Expression Plasmid of anti-Neurexin-1-Beta (Human). Derived from hybridoma N170A/1.1.

    Depositor

    James Trimmer

    Article

    Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

    Insert

    anti-Neurexin-1-Beta (Homo sapiens) recombinant mouse monoclonal antibody (NRXN1 Mouse)
    

    Expression

    Mammalian

    Promoter

    dual CMV

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. tdTomato-CD151-7

    Plasmid

    #58079

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Insert

    CD151 (CD151 Human)
    

    Tags

    tdTomato

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TJP1_PDZ_2

    Plasmid

    #103910

    Purpose

    Protein expression and purification of ZO1 PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    TJP1 (TJP1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL3-alphaMHC 3300bp

    Plasmid

    #51934

    Purpose

    luciferase reporter for rat alpha-MHC promoter

    Depositor

    Jeffery Molkentin

    Insert

    alpha MHC (Myh6 Rat)
    

    Use

    Luciferase

    Tags

    luciferase

    Mutation

    -2930 to +410 promoter region

    Promoter

    alpha MHC

    Available Since

    May 28, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pCMV-PARP1_D770A-mCherry

    Plasmid

    #192260

    Purpose

    Expresses PARP1 D770A mutant in mammalian cells. Tagged with mCherry.

    Depositor

    Michael Cohen

    Article

    Arnold et al Cell Chem Biol. 2022 Dec 15;29(12):1694-

    Insert

    PARP1_D770A (PARP1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D770A

    Promoter

    CMV

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-PARP1_R878A-mCherry

    Plasmid

    #192261

    Purpose

    Expresses PARP1 R878A mutant in mammalian cells. Tagged with mCherry.

    Depositor

    Michael Cohen

    Article

    Arnold et al Cell Chem Biol. 2022 Dec 15;29(12):1694-

    Insert

    PARP1_R878A (PARP1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    R878A

    Promoter

    CMV

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDONR223 LysoFlux (HaloTag7-mGFP-Galectin3-STOP)

    Plasmid

    #215003

    Purpose

    Shuttling vector for a Lysophagy reporter

    Depositor

    Wade Harper

    Insert

    LGALS3 (LGALS3 Human)
    

    Use

    Gateway

    Tags

    HaloTag(7)-mGFP

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. mOrange2-C1

    Plasmid

    #54650

    Purpose

    Localization: C1 Cloning Vector, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson, Roger Tsien

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Tags

    mOrange2

    Expression

    Mammalian

    Available Since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. lenti.Cas9.BFP.Blast

    Plasmid

    #196714

    Purpose

    WT SpCas9 with BSD-TagBFP for selection and sorting

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Promoter

    EF1a

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEX-GP-2-KDM4A double Tudor

    Plasmid

    #59699

    Purpose

    Contains histone modification interacting domain (KDM4A double Tudor)

    Depositor

    Albert Jeltsch

    Article

    Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

    Insert

    KDM4A double Tudor (KDM4A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Oct. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLEX305_3xFLAG_RELA1

    Plasmid

    #220324

    Purpose

    Expresses full length human RELA with an N-terminal 3xFLAG tag

    Depositor

    Jonathan Brown

    Insert

    RELA (RELA Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Promoter

    hPGK

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pUC18T-mini-Tn7T-Gm-dsRedExpress

    Plasmid

    #65032

    Purpose

    GmR mini-Tn7 vector, mobilizable, for RFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    dsRedExpress
    

    Expression

    Bacterial

    Available Since

    Aug. 14, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. TJP1_PDZ_1

    Plasmid

    #103909

    Purpose

    Protein expression and purification of ZO1 PDZ1 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    TJP1 (TJP1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pmCherry Paxillin

    Plasmid

    #50526

    Depositor

    Kenneth Yamada

    Insert

    PXN paxillin (PXN Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEGFP(C3)-Nup153

    Plasmid

    #64268

    Purpose

    GFP-tagged mammalian expression of Nup153

    Depositor

    Birthe Fahrenkrog

    Article

    Duheron et al Nucleus. 2014;5(6):601-12. doi: 10.4161/

    Insert

    NuP153 (NUP153 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. p5E-EF1a/b-actin

    Plasmid

    #82583

    Purpose

    5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expression

    Depositor

    Kryn Stankunas

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    EF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter
    

    Promoter

    None

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge