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pTU-A-013

Plasmid

#124415

Purpose

Slot A, empty (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot A + terminator + spacer

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pC-0_v3

Plasmid

#124423

Purpose

Full MCS (step 2)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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Mode #1 Library

Pooled Library

#111704

Purpose

Encodes GST-fused human phosphopeptides

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Expression

Bacterial

Species

Homo sapiens

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only
cmv-mCherry-8xPP7

Plasmid

#185795

Purpose

mCherry labeled with 8xPP7

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

cmv-mCherry-8xPP7

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pUCDMp10

Plasmid

#170461

Purpose

EarlyBac insect cell expression system

Depositor

Hiro Furukawa

Article

Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. do

Insert

New vector

Expression

Insect

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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cmv-mCherry-10xPP7

Plasmid

#185796

Purpose

mCherry labeled with 10xPP7

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

cmv-mCherry-10xPP7

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pQCH

Plasmid

#164160

Purpose

IncQ BHR plasmid

Depositor

Calvin Henard

Article

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pRAV24

Plasmid

#67716

Purpose

Parental plasmid for transcription of RNAs with a GlmS/M-domain 3'-tag for native purification

Depositor

Robert Batey, Jeffrey Kieft

Article

Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

Insert

Transcription cassette for 3'-tagged RNAs

Expression

Bacterial

Promoter

T7 RNA polymerase

Available Since

Sept. 8, 2015

Availability

Academic Institutions and Nonprofits only
pRAV23

Plasmid

#67715

Purpose

Parental plasmid for transcription of RNAs with a GlmS/MS2 3'-tag for native purification

Depositor

Robert Batey, Jeffrey Kieft

Article

Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju

Insert

Transcription cassette for 3'-tagged RNAs

Expression

Bacterial

Promoter

T7 RNA polymerase

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only
pJK131

Plasmid

#71548

Purpose

Produces Geobacillus stearothermophilus (Bacillus stearothermophilus) alanine racemase (alr)

Depositor

T. J. Kappock

Article

Francois et al Protein Expr Purif. 2007 Jan;51(1):39-48

Insert

Alanine racemase

Expression

Bacterial

Promoter

T7

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pC-0_v6

Plasmid

#124426

Purpose

Full MCS, lacks araC & P(BAD) (step 2)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTU-A-000

Plasmid

#124409

Purpose

Slot A, empty (step 1)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Insert

slot A + terminator + spacer

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pC-0_v4

Plasmid

#124424

Purpose

Full MCS, lacks araC & P(BAD) (step 2)

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al ACS Synth Biol. 2019 Jul 19;8(7):1691-16

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TarsierMER20MER39

Plasmid

#35261

Depositor

Gunter Wagner

Article

Emera et al Proc Natl Acad Sci U S A. 2012 Jul 10;10

Insert

MER20/MER39

Use

Luciferase

Available Since

March 20, 2012

Availability

Academic Institutions and Nonprofits only
orangutanMER20MER39

Plasmid

#35257

Depositor

Gunter Wagner

Article

Emera et al Proc Natl Acad Sci U S A. 2012 Jul 10;10

Insert

MER20/MER39

Use

Luciferase

Available Since

March 19, 2012

Availability

Academic Institutions and Nonprofits only
colobusMER20MER39

Plasmid

#35258

Depositor

Gunter Wagner

Article

Emera et al Proc Natl Acad Sci U S A. 2012 Jul 10;10

Insert

MER20/MER39

Use

Luciferase

Available Since

March 19, 2012

Availability

Academic Institutions and Nonprofits only
AtelesMER20MER39

Plasmid

#35259

Depositor

Gunter Wagner

Article

Emera et al Proc Natl Acad Sci U S A. 2012 Jul 10;10

Insert

MER20/MER39

Use

Luciferase

Available Since

March 19, 2012

Availability

Academic Institutions and Nonprofits only
CebusMER20MER39

Plasmid

#35260

Depositor

Gunter Wagner

Article

Emera et al Proc Natl Acad Sci U S A. 2012 Jul 10;10

Insert

MER20/MER39

Use

Luciferase

Available Since

March 19, 2012

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag RPS6KB1

Plasmid

#20624

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

RPS6KB1 (RPS6KB1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-FLEX-SomaGCaMP7

Plasmid

#182942

Purpose

Cre-dependent expression of cell body-targeted GCaMP7f, under CAG promoter. As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

Soma-GCaMP7

Use

AAV

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pT3-EF1A-MYC-IRES-luc

Plasmid

#129775

Purpose

Transposon based vector that expresses human MYC followed by IRES and firefly luciferase

Depositor

Amaia Lujambio

Article

Ruiz de Galarreta et al Cancer Discov. 2019 Jun 11. pii: 2159-82

Insert

human MYC (MYC Human)

Tags

luciferase

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pUCmini-iCAP-AAV9-X1.1

Plasmid

#196836

Purpose

non-standard AAV2 rep-AAV9-X1.1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

Insert

Synthetic construct isolate AAV9-X1.1 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe-Puro-BRAF-V600E

Plasmid

#15269

Purpose

Retroviral expression of BRAF V600E (the V600E mutation has been found in cancer patients, confers increased kinase activity, and is transforming)

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

BRAF (BRAF Human)

Use

Retroviral

Tags

His and Myc

Expression

Mammalian

Mutation

Valine 600 to Glutamate (mutation has been found …

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pCS2-Myc-TRIP12-CD

Plasmid

#241814

Purpose

Expresses Myc-tagged catalytic dead TRIP12 in mammalian cells.

Depositor

Ethan Lee

Article

Kassel et al Nat Commun. 2025 Jun 5;16(1):5248. doi:

Insert

TRIP12 (TRIP12 Human)

Tags

Myc

Expression

Mammalian

Mutation

catalytic dead (C2007A)

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Neo Myr Flag CKB

Plasmid

#20466

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CKB (CKB Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
piggyBac-rtTA (4th_Gen)-NGN2-2A-PURO-IRES-SNAP (VK_1018)

Plasmid

#209077

Purpose

Ngn2 mediated cortical neuron induction, dox induction is included

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Inserts

NGN2 (Neurog2 Mouse)
SNAPtag

Expression

Mammalian

Promoter

TRE4G

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag CMPK

Plasmid

#20537

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CMPK (CMPK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pET30-2-GPD1L

Plasmid

#83912

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

glycerol-3-phosphate dehydrogenase 1-like (GPD1L Human)

Tags

6x His tags

Expression

Bacterial

Promoter

T7

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET30-2-GAPDH

Plasmid

#83910

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

glyceraldehyde-3-phosphate dehydrogenase (GAPDH Human)

Tags

6x His tags

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pUCmini-iCAP-AAV1.X1

Plasmid

#209780

Purpose

non-standard AAV2 rep-AAV1-X1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Chen et al Nat Commun. 2023 Jun 8;14(1):3345. doi:

Insert

Synthetic construct isolate AAV1-X1 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Neo Myr Flag MAP2K5

Plasmid

#20514

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAP2K5 (MAP2K5 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-28a KGA-WT

Plasmid

#227846

Purpose

To express in E. coli KGA-WT

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

KGA isoform (GLS Human)

Tags

HIS

Expression

Bacterial

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-28a GAC-WT

Plasmid

#227845

Purpose

To express in E. coli GAC-WT

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

GAC isoform (GLS Human)

Tags

HIS

Expression

Bacterial

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRP-BRG1-HA-SmBiT

Plasmid

#241815

Purpose

Expresses HA-tagged and HiBiT-tagged BRG1 for use with the Promega HiBiT system

Depositor

Ethan Lee

Article

Kassel et al Nat Commun. 2025 Jun 5;16(1):5248. doi:

Insert

BRG1 (SMARCA4 Human)

Tags

HA and SmBiT

Expression

Mammalian

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Neo Myr Flag LIMK1

Plasmid

#20512

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

LIMK1 (LIMK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

aa1-273 only

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJM5-WT PHGDH

Plasmid

#83901

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

Phosphoglycerate dehydrogenase (PHGDH Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))

Plasmid

#105842

Purpose

Donor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichment

Depositor

Michael Ward

Article

Fernandopulle et al Curr Protoc Cell Biol. 2018 Jun;79(1):e5

Inserts

Neurogenin 2 (NEUROG2 Human)
ISL LIM Homeobox 1 (ISL1 Human)
LIM Homeobox 3 (LHX3 Human)
Optimized mApple
rtTA3G
SBP Delta-LNGFR

Use

CRISPR and TALEN

Promoter

CAG, EF-1alpha, and TRE3G

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA 3.3 C-FLAG PDZD8-WT

Plasmid

#227838

Purpose

Used for transfection of cells for expression of C-FLAG PDZD8-WT

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

PDZD8 (PDZD8 Human)

Tags

FLAG

Expression

Mammalian

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX4T-1 PDZD8-WT

Plasmid

#227849

Purpose

To express in E. coli PDZD8-WT

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

PDZD8 (PDZD8 Human)

Tags

GST

Expression

Bacterial

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
elavl3:SomaGCaMP7f

Plasmid

#158760

Purpose

Cell body-targeted GCaMP7f, under HuC promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.

Depositor

Edward Boyden

Article

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP7

Use

Synthetic Biology

Promoter

HuC promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpp1-is2

Plasmid

#58248

Purpose

Expression vector producing isoform 2 derived mouse osteopontin (EGFP tagged)

Depositor

Georgios Stathopoulos

Article

Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

Insert

Spp1-is2 (Spp1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Neo Myr Flag LCK

Plasmid

#20511

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

LCK (LCK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA 3.3 N-MYC EPHA2

Plasmid

#227879

Purpose

Used for transfection of cells for expression of C-MYC EPHA2

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

EPHA2 (EPHA2 Human)

Tags

MYC

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-TNT4-Zfr-P2A-HA-YAP5SA-3xMIR122

Plasmid

#223186

Purpose

Plasmid containing the Zfr/YAP5SA/3xMIR122 cassette. Translation of YAP5SA is controlled by splicing modulation of the Zfr specifically in cardiomyocytes by risdiplam in vivo.

Depositor

Yuxuan Guo

Article

Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

Inserts

YAP5SA (Yap1 Mouse)
mir122 (Mir122 Mouse)

Use

AAV

Tags

HA and engineered P2A

Mutation

S61A, S109A, S127A, S164A, S381A

Promoter

TNNT2

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pExp-His-zBasic-RBD

Plasmid

#194999

Purpose

Production of SARS-CoV2 receptor binding domain in E. coli

Depositor

Marko Hyvönen

Article

Gaynor et al Nat Commun. 2023 Jun 16;14(1):3583. doi:

Insert

RBD (S SARS-CoV-2)

Tags

His8-Zbasic

Expression

Bacterial

Promoter

T7lac

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpp1-is3

Plasmid

#58247

Purpose

Expression vector producing isoform 3 derived mouse osteopontin (EGFP tagged)

Depositor

Georgios Stathopoulos

Article

Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

Insert

Spp1 isoform 3 (Spp1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pWZL Neo Myr Flag BTK

Plasmid

#20432

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

BTK (BTK Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pET30-2-GPD1

Plasmid

#83911

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

glycerol-3-phosphate dehydrogenase 1 (GPD1 Human)

Tags

6x His tags

Expression

Bacterial

Promoter

T7

Available Since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
sgTP53_3

Plasmid

#78164

Purpose

sgTP53

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

TP53 (TP53 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag CAMK2B

Plasmid

#20439

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CAMK2B (CAMK2B Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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