We narrowed to 18,161 results for: Met;

1. pHPHS800

   Plasmid

   #228240

   Purpose

   Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination

   Depositor

   Arvind Rasi Subramaniam

   Article

   Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.

   Insert

   Cas9
   

   Use

   Tags

   Hygromycin phosphotransferase

   Expression

   Mammalian

   Mutation

   Promoter

   none

   Available since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a-hfYFP-TEV-Bcx

   Plasmid

   #191195

   Purpose

   Fusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   hfYFP & Bcx fusion
   

   Use

   Tags

   6xHis and Bacillus circulans xylanase

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PTet-F30-Pepper aptamer-UTR1-mTurq2

   Plasmid

   #227645

   Purpose

   pTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.

   Depositor

   Richard Murray

   Article

   Kapasiawala et al bioRxiv 2023.03.22.533877

   Insert

   F30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pETM6-T7-VHH-GFPminimizer

   Plasmid

   #202488

   Purpose

   Expresses the nanobody GFP minimizer with T7 induction

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   nanobody GFP minimizer
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSMART-HCKan-yibDp-VHH72

   Plasmid

   #202467

   Purpose

   Expresses the nanobody VHH72 after phosphate depletion

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   nanobody VHH72
   

   Use

   Tags

   6X-His

   Expression

   Bacterial

   Mutation

   Promoter

   yibDp

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSMART-HCKan-yibDp-VHH-H6antivenin

   Plasmid

   #202470

   Purpose

   Expresses the nanobody H6 antivenin after phosphate depletion

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   nanobody H6 antivenin
   

   Use

   Tags

   6X-His

   Expression

   Bacterial

   Mutation

   Promoter

   yibDp

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSMART-HCKan-yibDp-VHH-1B5

   Plasmid

   #202472

   Purpose

   Expresses the nanobody 1B5 after phosphate depletion

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   nanobody 1B5
   

   Use

   Tags

   6X-His

   Expression

   Bacterial

   Mutation

   Promoter

   yibDp

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSMART-HCKan-yibDp-VHH-2E7

   Plasmid

   #202473

   Purpose

   Expresses the nanobody 2E7 after phosphate depletion

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   nanobody 2E7
   

   Use

   Tags

   6X-His

   Expression

   Bacterial

   Mutation

   Promoter

   yibDp

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSMART-HCKan-yibDp-VHH-3E3

   Plasmid

   #202474

   Purpose

   Expresses the nanobody 3E3 after phosphate depletion

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   nanobody 3E3
   

   Use

   Tags

   6X-His

   Expression

   Bacterial

   Mutation

   Promoter

   yibDp

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSMART-HCKan-yibDp-VHH-C9BoNT-A

    Plasmid

    #202475

    Purpose

    Expresses the nanobody C9 BoNT-A after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody C9 BoNT/A
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSMART-HCKan-yibDp-VHH-E7BoNT-A

    Plasmid

    #202476

    Purpose

    Expresses the nanobody E7 BoNT-A after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody E7 BoNT/A
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSMART-HCKan-yibDp-VHH-JM3

    Plasmid

    #202477

    Purpose

    Expresses the nanobody JM3 after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody JM3
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSMART-HCKan-yibDp-VHH-J3

    Plasmid

    #202478

    Purpose

    Expresses the nanobody J3 after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody J3
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSMART-HCKan-yibDp-VHH-m36.4

    Plasmid

    #202479

    Purpose

    Expresses the nanobody m36.4 after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody m36.4
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSMART-HCKan-yibDp-VHH-Re5D06

    Plasmid

    #202480

    Purpose

    Expresses the nanobody Re5D06 after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody Re5D06
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSMART-HCKan-yibDp-VHH-Re9F06

    Plasmid

    #202481

    Purpose

    Expresses the nanobody Re9F06 after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody Re9F06
    

    Use

    Tags

    6X-His

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCOLA-Gent-ppiB

    Plasmid

    #202482

    Purpose

    Expresses peptidyl-prolyl cis-trans isomerase (ppiB) after phosphae depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    peptidyl-prolyl cis-trans isomerase (ppiB)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    yibDp

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCASCADE-LCAmp-trxB

    Plasmid

    #202465

    Purpose

    Expresses a guide RNA to silence trxB after phosphate depletion

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    trxB guide RNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ugpB

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMV261-igr3WT

    Plasmid

    #225738

    Purpose

    To express wild type igr3 (Rv3542c-Rv3541c-Rv3540c) in Mycobacteria

    Depositor

    Nicole Sampson

    Article

    Panagoda et al ACS Chem Biol. 2024 Dec 20;19(12):2580-2

    Insert

    Myc-igr3WT
    

    Use

    Tags

    c-Myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-MCS-3AID-HA-2A-mTurquoiseBSD

    Plasmid

    #225704

    Purpose

    Empty backbone for lentiviral expression of AID degron tagged POI and mTurquoise fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Tags

    T2A-mTurquoise

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1A

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLenti-MCS-3AID-HA-2A-mCherryBSD

    Plasmid

    #225700

    Purpose

    Empty backbone for lentiviral expression of AID degron tagged POI and mCherry fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Tags

    T2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    pEF1A

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLenti-rTetR(SE)-VP64-2A-mCherryBSD

    Plasmid

    #225695

    Purpose

    Lentiviral expression of rTetR(SE) fused to VP64 and mCherry fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    rTetR-VP64
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xFLAG and T2A-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLenti-rTetR(SE)-KRAB-2A-mTurquoise

    Plasmid

    #225690

    Purpose

    Lentiviral expression of rTetR(SE) fused to ZNF10 KRAB and mTurquoise-NLS

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    rTetR-ZNF10 KRAB
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xFLAG and T2A-mTurquoise

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGL4.23_Ptgs2

    Plasmid

    #209519

    Purpose

    Expresses luciferase under control of Ptgs2 promoter in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Khader et al PLoS One. 2024 Apr 18;19(4):e0297847. do

    Insert

    Ptgs2 (Ptgs2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAc5.1-PH-mCherry-MARF1 isoform D (HK56)

    Plasmid

    #206470

    Purpose

    MARF1 expression in Schneider cells for imaging

    Depositor

    Mandy Jeske

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Insert

    MARF1 (CG17018 Fly)
    

    Use

    Tags

    PH mcherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAF201

    Plasmid

    #222376

    Purpose

    Recombinant Tasmanian devil IgG antibody (1F8) that binds to Tasmanian devil PDL1

    Depositor

    Andrew S. Flies

    Article

    Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

    Insert

    IGHG
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAF200

    Plasmid

    #222375

    Purpose

    Recombinant Tasmanian devil IgG antibody (3D5) that binds to 6xHis at the C-terminus of proteins

    Depositor

    Andrew S. Flies

    Article

    Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

    Insert

    IGHG
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti6.3-Nm2Cas9-BlastR

    Plasmid

    #220980

    Purpose

    Expresses human codon-optimized Nm2Cas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    Nm2Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLenti6.3-CjCas9-BlastR

    Plasmid

    #220979

    Purpose

    Expresses human codon-optimized CjCas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    CjCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLenti6.3-St1Cas9-BlastR

    Plasmid

    #220976

    Purpose

    Expresses human codon-optimized St1Cas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    St1Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLenti6.3-SlugCas9-HF-BlastR

    Plasmid

    #220973

    Purpose

    Expresses human codon-optimized SlugCas9-HF

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    SlugCas9-HF
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLenti6.3-SauriCas9-KKH-BlastR

    Plasmid

    #220972

    Purpose

    Expresses human codon-optimized SauriCas9-KKH

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    SauriCas9-KKH
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLenti6.3-Sa-SlugCas9-BlastR

    Plasmid

    #220968

    Purpose

    Expresses human codon-optimized Sa-SlugCas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    Sa-SlugCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAc5.1-PH-mCherry-Roquin isoform A (HK63)

    Plasmid

    #206469

    Purpose

    Roquin expression in Schneider cells for imaging

    Depositor

    Mandy Jeske

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Insert

    Roquin (roq Fly)
    

    Use

    Tags

    PH mcherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAc5.1-mEGFP-CAF1 isoform A (HK50)

    Plasmid

    #206437

    Purpose

    CAF1 expression in Schneider cells for imaging

    Depositor

    Mandy Jeske

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Insert

    CAF1 (Pop2 Fly)
    

    Use

    Tags

    mEGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAc5.1-mEGFP-CAF40 isoform A (HK51)

    Plasmid

    #206438

    Purpose

    CAf40 expression in Schneider cells for imaging

    Depositor

    Mandy Jeske

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Insert

    CAf40 (Rcd-1 Fly)
    

    Use

    Tags

    mEGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAc5.1-mEGFP-human FRB (HK168)

    Plasmid

    #206443

    Purpose

    FRB expression in Schneider cells for imaging

    Depositor

    Mandy Jeske

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Insert

    FRB (MTOR Human)
    

    Use

    Tags

    mEGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAc5.1-λN-HA-CAF1 isoform A (HK101)

    Plasmid

    #206448

    Purpose

    CAF1 expression in Schneider cells for imaging

    Depositor

    Mandy Jeske

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Insert

    CAF1 (Pop2 Fly)
    

    Use

    Tags

    lambda N HA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAc5.1-PH-mCherry-CAF40 isoform A (HK97)

    Plasmid

    #206455

    Purpose

    CAF40 expression in Schneider cells for imaging

    Depositor

    Mandy Jeske

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Insert

    CAF40 (Rcd-1 Fly)
    

    Use

    Tags

    PH mcherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRetroX-TRE3G-Tir

    Plasmid

    #221326

    Purpose

    Dox-inducible expression of Tir in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    Tir
    

    Use

    Retroviral

    Tags

    HN-FLAG

    Expression

    Mutation

    Promoter

    TRE3GV

    Available since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge