-
Plasmid#201150PurposeAAV virus production for Cre recombinase dependent expression of eGCaMP(2+)DepositorInserteGCaMP_2+
UseAAV, Cre/Lox, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV/EF1a-DIO-SCLM
Plasmid#216759PurposeAAV2 transfer vector for Cre recombinase-dependent expression of SuperClomeleon from the human elongation factor 1a promoterDepositorInsertSuperClomeleon
UseAAVTagsExpressionMammalianMutationS30R and 11 AA deletion in the Cerulean CFP moiet…PromoterEF1a (human elongation factor 1 alpha)Available sinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
LV_EF1a_KRAS(G12V)-P2A-Hygro_Barcode
Plasmid#170237PurposeBarcoded lentiviral vector to express KRAS (G12V) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorInsertKRAS (KRAS Human)
UseLentiviralTagsExpressionMutationG12VPromoterEF1aAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1a-jGCaMP8m-WPRE
Plasmid#176757PurposeAAV-mediated expression of GCaMP8m under the EF1a promoterDepositorHas ServiceAAV Retrograde, AAV1, and AAV9InsertjGCaMP8m
UseAAVTags6xHisExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-extGluc-NGFR
Plasmid#104832PurposeextGluc luciferase for invivo Imaging, NGFR for flourescent detection or separationDepositorInsertsextGLuc
NGFR
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1a-MegaGate-DD-Hygro
Plasmid#175267PurposeMegaGate destination with C-terminal DD tag and hygromycin resistanceDepositorTypeEmpty backboneUseTagsDestabilization DomainExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4
Plasmid#98975PurposeCas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 4 and Cas9(D10A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-flex-TVA
Plasmid#69618PurposeExpress recepter TVA for envA with AAVDepositorInsertAvian sarcoma and leukemia virus receptor 950 (CD320 Synthetic, quail)
UseAAV and Cre/LoxTagsFLAGExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1a-BCL2-hygro
Plasmid#222581PurposePiggyBac transposon plasmid for constitutive expression of BCL2DepositorInsertBCL2 (BCL2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-PGD2-1.1
Plasmid#214764PurposeAAV packaging plasmid for GRAB PGD2-1.1 fluorescent sensor under EF1alpha promoterDepositorInsertGRAB-PGD2-1.1 sensor
UseAAVTagsExpressionMutationPromoterEF-1-alpha promoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1A-DIO-mRuby2
Plasmid#126215PurposeLabeling of cre-expressing neurons (red fluorescence)DepositorInsertmRuby2
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1AAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-EF1a-mTagRFP-T-CAAX
Plasmid#167973PurposeAAVS1 targeting repair templateDepositorUseCRISPR; Donor templateTagsmTagRFP-T-CAAXExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
IF101: pMVP (L1-L4) EF1a promoter
Plasmid#121687PurposepMVP L1-L4 entry plasmid, contains EF1a promoter for 3-component MultiSite Gateway Pro assemblyDepositorInsertEF1a promoter
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
miniTol2 x EF1a_EKAREN5 + PGK-blast
Plasmid#167818PurposeOptimized EKAREV FRET biosensor sensor for ERKDepositorInsertEKAREN5
UseTagsnls localization motifExpressionMammalianMutationK424P, K426WPromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1
Plasmid#98972PurposeCas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 1 and Cas9(D10A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV x EF1a_EKAREN4-ires-puro
Plasmid#167828PurposeOptimized EKAREV FRET biosensor sensor for ERKDepositorInsertEKAREN4
UseLentiviralTagsnls localization motifExpressionMammalianMutationK424P, K426WPromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRRLNeo-pEF1a-p53ashL344P-mCerulean
Plasmid#69581PurposeExpresses p53-L344P mutant tagged with mCeruleanDepositorInsertp53-L344P (TP53 Human)
UseLentiviralTagsmCeruleanExpressionMammalianMutationp53 L344P mutation that prevents oliogomerization…PromoterEF1alphaAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-HTB
Plasmid#44187DepositorInsertHistone tagged GFP, T2A-TVA-E2A-B19G
UseAAV; Adeno-associated vectorTagsGFPExpressionMutationPromoterEF1aAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TALEN_HD (SPC right)
Plasmid#126695Purposeright TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start siteDepositorInserthuman SPC right
UseTALENTagsNLS, truncated N term and TAL, FokIExpressionMammalianMutationR451P in SPCPromoterEF1Available sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TALEN_NN (SPC left)
Plasmid#126694Purposeleft TALENS for introducing a double strand break into the genomic human SFTPC locus at the ATG start siteDepositorInserthuman SPC left
UseTALENTags0.5 repeat NN, TAL, FokI and NLS, truncated N ter…ExpressionMammalianMutationPromoterEF1Available sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2
Plasmid#98973PurposeCas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 2 and Cas9(D10A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-GFP ArgSen
Plasmid#184695PurposeLentiviral transfer plasmid for expression of GFP ArgSenDepositorInsertGFP ArgSen
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-CD19.8H.28TM.28.BB.3z
Plasmid#200676PurposeModular CD8hinge-CD28transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral ProductionDepositorUseLentiviralTagsMycTagExpressionMammalianMutationPromoterEF1a-shortAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a CHR2 mCherry WPRE
Plasmid#129432Purposeexpression of CHR2 mCherry from EF1a promoterDepositorInsertCHR2 mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lv EF1a MS2-Fkbp1x 2A Hygro
Plasmid#102806PurposeLentiviral plasmid from FIRE-Cas9 system to express MS2-Fkbp1x fusion proteinDepositorInsertMS2-Fkbp
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
miniTol2 x EF1a_EKAREN5(TA) + PGK-puro
Plasmid#167819Purposecontrol sensor for EKAREN5DepositorInsertEKAREN5(TA)
UseTagsnls localization motifExpressionMammalianMutationK424P, K426W, T420APromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV Ef1a-mRuby3-WPRE
Plasmid#135427PurposeCan be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.DepositorInsertmRuby3
UseAAVTagsExpressionMammalianMutationPromoterEf1aAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-GFP RanSen
Plasmid#184705PurposeLentiviral transfer plasmid for expression of GFP RanSenDepositorInsertGFP RanSen
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
miniTol2 x EF1a_EKAREN4(TA) + PGK-blast
Plasmid#167827PurposeControl sensor for EKAREN4DepositorInsertEKAREN4(TA)
UseTagsnls localization motifExpressionMammalianMutationK424P, K426W, T420APromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHL-EF1a-SphcCas9(D10A)-iP-A
Plasmid#60600PurposeExpresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.DepositorInsertsCRISPR Cas9 D10A
puromycin resistance gene
UseCRISPRTagsExpressionMammalianMutationChanged Asp 10 to Ala, Codon usage optimized for …PromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only